Karakterisasi dan Identifikasi Bakteri Enterobacteriaceae pada Telur Penyu Lekang (Lepidochelys olivacea) asal Lhok Pante Tibang, Banda Aceh

https://doi.org/10.22146/jsv.48514

Risa Nursanty *, Widya Sari, Safranita Risa Nursanty *, Widya Sari, Safranita(1*), Widya Sari(2), Safranita Safranita(3)

(1) Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Syiah Kuala
(2) Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Syiah Kuala
(3) Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Syiah Kuala
(*) Corresponding Author

Abstract


The population of olive ridley turtle (Lepidochelys olivacea) are currently thretened with extinction, among the contributing factors is the failure of turtle eggs to hatch. The condition can be caused by the presence of bacterial contamination, especially group Enterobacteriaceae. The purpose of this research was to isolate the group of Enterobacteriaceae bacteria from olive ridley turtle. The sample used were shells from olive ridley turtle eggs comes from natural captivity in Lhok Pante Tibang Beach, Banda Aceh. The research parameters included the number of isolates, macroscopic morphology (shape, edge, elevation, and color of isolates), cell morphology (Gram staining and cell shape), and biochemical tests. Isolation method using spread plate and quadrant method. The isolation results obtained five isolates which had varying macroscopic morphology. The five isolates were Gram negative and formed such as trunk. Identification results found that there were three genus from Enterobacteriaceae videlicet two isolates were Enterobacter, two isolates as Shigella and the other were Klebsiella.  

 


Keywords


Isolate, Enterobacteriaceae; Lepidochelys olivacea shells; Lhok Pante Tibang

Full Text:

PDF


References

Adnyana, I. B. W., dan Creausa, H. (2009). Panduan Melakukan Pemantauan Populasi Penyu di Pantai Peneluran di Indonesia. WWF, Indonesia dan Universitas Udayana.

Borrow, G. I., and Filtham, R. K. A. (2003). Cowan and Steel's Manual For Identification of Medical Bacteria. Cambrige University Press, USA.

Brooks, G. F., Butel, J. S., and Morse, S. A. (2008). Medical Microbiology. Mc Graw Hill, New York.

Cox, N. A., Berray, M. C., and Cason, J. A. (2000). Salmonella penetration of egg shells and proliferation in broiler hatching eggs A Review. Poultry Science. 79: 1571-1574.

Craven, J. E., Awong-Taylor, J., Griffiths, L. Bass, C., and Muscarella, M. (2007). Identification of bacteria isolates from unhatched loggerhead (Caretta caretta) sea turtles eggs in Georgia. Marine Turtle Newsletter. 115:7-9.

Dermawan, A., Nuitja, N. S., Soedharma, D., Halim, M. H., Kusrini, M. D., Lubis, S. B., Alhanif, R., Khazali, M., Murdiah, M., Wahjuhardini, P. L., Setiabudiningsih dan Mashar, A. (2009). Pedoman Teknis Pengelolaan Konservasi Penyu. Direktorat Konservasi dan Taman Nasional Laut, Direktorat Jenderal Kelautan, Pesisir dan Pulau-Pulau Kecil, Departemen Kelautan dan Perikanan RI, Indonesia.

Hatta, M. (2016). Pengaruh Karakteristik Pantai dan Naungan Sarang Terhadap Penetasan Telur Penyu Lekang (Lepidochelys olivacea) di Pantai Syiah Kuala, Banda Aceh dan Leupung, Aceh Besar. Skripsi. Fakultas Kelautan dan Perikanan, Universitas Syiah Kuala.

Hidayat, O., Fuji, A. F., dan Nasril, N. (2014). Isolasi dan Karakterisasi Bakteri pada Pasir Sarang dan Cangkang Telur Penyu lekang (Lepidochelys olivaceae L.) yang Menetas dan Gagal Menetas. Jurnal Biologi Universitas Andalas. 3 (2): 154-161.

Jawetz, E., Melnick, J. L., and Adelberg, E. A. (2004). Mikrobiologi Kedokteran. Terjemahan dari th Medical Micrbiology, 23 ed oleh Mudihardi, E., Kuntaman, Wasito, E. B., Mertaniasih, N. M., Harsono, S., dan Alimsardjono, L. Buku Kedokteran EGC, Jakarta.

Keen, E. L. (2012). Microorganism from sand, cloacal fluid and eggs of Lepidochelys olivacea and standard testing of cloacal fluid antiicrobial properties. Thesis. Purdue University. USA. Karakterisasi dan Identifikasi Bakteri Enterobacteriaceae pada Telur Penyu Lekang (Lepidochelys olivacea) ...

Morais, P. Benevides de., Oliveira, K. Wessel de., Malvasio, A., Ataide, A. Gomes de., and Pimenta, R. S. (2010). Enterobacteriaceae Associated With Eggs of Podocnemis expansa and Podocnemis unifilis (Testudines: Chelonia) In Nonpolluted Sites of National Park of Araguaia Plains, Brazil. Journal of Zoo and Wildlife Medicine. 41 (4): 656–66.

Phillott, A. D., and Parmenter, C. J. (2001). Influence of Diminished Respiratory Surface Area on Survival of Sea Turtle Embryos. Journal Xperimental Zoology. 289: 317–321.

Prescott, L. M., Harley, J. P., and Klein, D. A. (2002). Microbiology Fifth Edition. The McGraw-Hill Companies, New York. Rofiah, A., Retno, H., dan Edy, W. (2012). Pengaruh Naungan Sarang Terhadap Persentase Penetasan Telur Penyu lekang (Lepidochelys olivacea) di Pantai Samas Bantul, Yogyakarta. Journal of Marine Research. 1(2): 103-108.

Rudiana, E., Dwi, H. I., dan Nirwani, S. (2004) .Tingkat Keberhasilan Penetasan dan Masa Inkubasi Telur Penyu Hijau (Chelonia mydas

L) pada Perbedaan Waktu Pemindahan. Jurnal Ilmu Kelautan UNDIP. 9 (4): 202 – 205.

Sari, W dan Rahmad. (2014). Pemanfaatan Penyu oleh Masyarakat Pesisir di Kota Banda Aceh dan Kabupaten Aceh Besar. Prosiding. Seminar Nasional Biotik 2014. UIN Ar Raniry. Banda Aceh. 17-18 Maret 2014.

Satriadi, A., 2003). Identifikasi Esti, R., dan Nurul, A. ( Penyu dan Studi Karakteristik Fisik Habitat Penelurannya di Pantai Samas, Kabupaten Bantul, Yogyakarta. Jurnal Ilmu Kelautan UNDIP. 8 (2): 69 – 75.

Sunatmo, T. I. (2009). Mikrobiologi Esensial. Ardy Agency, Jakarta. Syaiful, N. B., Jabang, N., dan Indra, J. Z. (2013). Penetasan Telur Penyu lekang (Lepidochelys olivacea Eschscholtz, 1829) pada Lokasi Berbeda di Kawasan Konservasi Penyu Kota Pariaman. Jurnal Biologi Universitas Andalas. 2 (3): 175-180.



DOI: https://doi.org/10.22146/jsv.48514

Article Metrics

Abstract views : 5590 | views : 66867

Refbacks

  • There are currently no refbacks.




Copyright (c) 2019 Jurnal Sain Veteriner

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Sain Veteriner Indexed by

    CrossrefROADCOREProduct DetailsDESKRIPSI GAMBAR


Copyright of JSV (Jurnal Sain Veteriner) ISSN 0126-0421 (print), ISSN 2407-3733 (online).

Fakultas Kedokteran Hewan, Universitas Gadjah Mada

Jl. Fauna No.2, Karangmalang, Yogyakarta

Phone: 0274-560862

Fax: 0274-560861

Email: jsv_fkh@ugm.ac.id

HP. 0895363078367

Jurnal Sain Veteriner is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 

free
web stats View My Stats