Virulence Characteristic of Avian Pathogenic Escherichia coli (APEC) Isolates

Sruti Listra Adrenalin(1*), Lynda Nugrahaning Imanjati(2), Ima Fauziah(3), Vinsa Cantya Prakasita(4), Sitarina Widyarini(5), Agnesia Endang Trihastuti Wahyuni(6)

(1) Brawijaya University
(2) Gadjah Mada University
(3) Gadjah Mada University
(4) Duta Wacana Christian University
(5) Gadjah Mada University
(6) Gadjah Mada University
(*) Corresponding Author


Avian pathogenic Escherichia coli (APEC) is a cause of colibacillosis in poultry, one of the respiratory disease that causes serious problems in the poultry industry. The APEC can cause high mortality and culling, decreased production, and high costs of treatment. Manifestations of colibacillosis are airsacculitis, perihepatitis, and pericarditis. The APEC serotypes that are widely identified in the field are O1K1, O2K1, and O78K80. Embryo lethality assay (ELA) is a method for determine the virulence of APEC serotypes. The aim of this study was to determine the virulence characteristic of APEC isolates. Five APEC serotypes O1K1, O2K1, O78K80, O157H7, and unknown serotype were used for ELA method by inoculated E. coli into chorioallantoic of specific pathogen free 12-days old embryos. Each group of 10 embryos, inoculated E. coli dose of 100-500 CFU/ 0,1 ml. Candling was carried out for 6 days (18-days old embryo) to determined the mortality and pathological lesions. The percentage of embryo mortality post-inoculated with APEC O1K1, O2K1, unknown serotypes were 100% (10/10), O78K80 serotype was 90% (9/10), and O157H7 serotype was 70% (70%). Lesions of all embryos were cranial and extremity hemorrhage. In this study, E. coli isolates had high virulence.



avian pathogenic E. coli (APEC); E. coli serotypes; virulence; embryo lethality assay.

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Abbot, S.L., O’Connor, J., Robin, T., Zimmer, B.L., Janda, J.M. (2003). Biochemical Properties of a Newly Described Escherichia Species, Escherichia albertii. Journal of Clinical Microbiology 41: 4852-4854. Ahmed, H.A., Ali A.H.M., Baky M.H.A. (2015). Efficacy of a Live Escherichia coli Vaccine for Protection of Turkeys Against Homologous and Heterologous Field Strains Infection. Benha Veterinary Medical Journal 29(2): 11-16. Andersson, C., Gripenland, J., Johansson, J. (2015). Using the Chicken Embryo to Assess Virulence of Listeria monocytogenes and to Model Other Microbial Infections. Nature Protocols 10(8): 1155-1164. Chansiripornchai, V.R.N. (2009). The Efficacy of Escherichia coli AroA-Live Vaccine in Broilers Against Avian E. coli Serotype O78 Infection. Thai J. Vet. Med 39(4): 337-342. Chansiripornchai, N., Mooljuntee, S., Boonkhum, P. (2011). Antimicrobial Sensitivity of Avian Pathogenic Escherichia coli (APEC) Isolated from Chickens During 2007-2010. Thai J Vet 41(4): 519-522. Cortes, P., Blanc, V., Mora, A., Dahbi, G., Blanco, J.E., Blanco, M., Lopez, C., Andreu, A., Navarro, F., Alonso, M.P., Bou, G., Blanco, J., Llagostera, M. (2010). Isolation and Characterization of Potentially Pathogenic Antimicrobial-Resistant Escherichia coli Strains from Chicken and Pig Farms in Spain. Appl Environ Microbiol 76: 2799-2805. Dho-Moulin, M., Fairbrother, J.M. (1999). Avian Pathogenic Escherichia coli (APEC). Veterinary Research 30: 299-316. Dutta P., Borah, M.K., Sarmah, R., Gangil, R. (2013). Isolation, Histophatology, and Antibiogram of Escherichia coli from Pigeons (Columba livia). Vet World 6(2): 91-94. El-Sawah, A., Dahshan, A.H.M., El-Nahass, S., El-Mawgoud, A.I.A. (2018). Pathogenicity of Escherichia coli O157 in Commercial Broiler Chickens. Beni-Suef University Journal of Basic and Applied Sciences xxx(xxx): xxx-xxx. Ghunaim, H., Abu-Madi, M.A., Kariyawasam, S. (2014). Advances in Vaccination Against Avian Pathogenic Escherichia coli Respiratory Disease: Potentials and Limitations. Veterinary Microbiology 172: 13-22. Gregersen, R.H., Christensen, H., Ewers, C., Bisgaard, M. (2010). Impact of Escherichia coli Vaccine on Parent Stock Mortality, First Week Mortality of Broilers and Population Diversity of E. coli in Vaccinated Flocks. Avian Pathology 39(4): 287-295. Gibbs, P.S., Maurer, J.J., Nolan, L.K., Wooley, R.E. (2003). Prediction of Chicken Embryo Lethality with the Avian Escherichia coli Traits Complement Resistance, Colicin V Production, and Presence of the Increased Serum Survival Gene Cluster (iss). Avian Disease 47: 370-379. Gordon, R.F., Jordan, F.T.W. (1982). Poultry Disease. 2nd Edition. The English Language Book Society and Bailliere Tindall. London. pp 31-37. Gross, W.B. (1991). Colibasillosis. In: Diseases of poultry 9th Edition. Iowa State University Press, Ames, Iowa 138-144. Kemmett, K., Humphrey, T., Rushton, S., Close, A., Wigley, P., Williams, N.J. (2013). A Longitudinal Study Simultaneously Exploring thr Carriage of APEC Virulence Associated Genes and The Molecular Epidemiology of Faecal and Systemic E. coli in Commercial Broiler Chicken. Plos One 8: e67749. La Ragione, R.M.,Woodward, M.J., Kumar, M., Rodenberg, J., Fan, H., Wales, A.D., Karaca, K. (2013). Efficacy of a Live Attenuated Escherichia coli O78 and K80 Vaccine in Chickens and Turkeys. Avian Dis 57: 273-279. Nabbut, N., Khatib, I. (1978). Virulence of Escherichia coli Strains for Chicken Embryos. Avian Dis 22: 10-15. Nolan, L.K., Wooley, R.E., Brown, J., Spears, K.R., Dickerson, H.W., Dekich, M. (1992). Comparison of a Complement Resistance Test, a Chicken Embryo Lethality Test, and the Chicken Lethality Test for Determining Virulence of Avian Escherichia coli. Avian Disease 36: 395-397. Nataro, J.P., Bopp, C.A., Fields, P.I., Kaper, J.B., Strockbine, N.A. (2011). Escherichia, Shigella, and Salmonella. In: Manual of Clinical Microbiology, 10th ed Vol 1. Versalovic, J., Carroll, K.C., Funk, G., Jorgensen, J.H., Landry, M.L., Warnock, D.W. eds ASM Press, Washingon D.C. 603-626. Oh, J.Y., Kang, M.S., Yoon, H., Choi, H.W., An, B.K., Shin, E.G., Kim, Y.J., Kim, M.J., Kwon, J.H., Kwon, Y.K. (2012). The Embryo Lethality of Escherichia coli Isolates and Its Relationship to the Presence of Virulence-Associated Genes. Poultry Science 91: 370-375. Ozaki, H., Murase, T. (2009). Multiple Routes of Entry for Escherichia coli Causing Colibacillosis in Commercial Layer Chickens. J. Vet. Med. Sci 71(12): 1685-1689. Powell, C., Finkelstein, R. (1966). Virulence of Escherichia coli Strains for Chick Embryos. J. Bacterial 91: 1410-1417. Roseliza, R., Khairani-Bejo, S., Zunita, Z., Ramlan, M., Khoo, E., Rosnah, Y. (2016). Antibiotic Resistence of Escherichia coli Isolated From Chicken in Malaysia. Malaysian Journal of Veterinary Research 7(1): 65-76. Swayne, D.E., Glisson, J.R., Larry, R., McDougald, Nolan, L.K., David, L., Suarez, Nair, V. (2013). Disease of Poultry 13th Edition. Wiley Blackwell Publication. Wahyuwardani, S., Noor, S.M., Poeloengan, M., Andriani, Aryanti, T. (2014). Kasus Kolibasilosis pada Peternakan Ayam Pedaging di Yogyakarta dan Bogor. Seminar Nasional Teknologi Peternakan Dan Veteriner. Wibowo, M.H., Amanu, S. (2009). Efektivitas Pengobatan Preparat Kombinasi Amoksisilin dan Kolistin Sulfat pada Kasus Infeksi Buatan Escherichia coli Patogen pada Ayam Broiler. J. Sain Vet 27(1): 1-9. Wooley, R.E., Gibbs, P.S., Brown, T.P., Maurer, J.J. (2000). Chicken Embryo Lethality Assay for Determining the Virulence of Avian Escherichia coli Isolates. Avian Disease 44: 318-324.


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