Pengaruh Infusa Biji Buah Pinang (Areca catechu) Terhadap Tingkat Kematian dan Morfometri Cacing Dewasa Ascaridia galli Secara In Vitro

https://doi.org/10.22146/jsv.43751

Wida Wahidah Mubarokah(1*), Kurniasih Kurniasih(2), Wisnu Nurcahyo(3), Joko Prastowo(4)

(1) Politeknik Pembangunan Pertanian Yogyakarta Magelang
(2) Departemen Pathologi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Yogyakarta
(3) Departemen Parasitologi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Yogyakarta
(4) Departemen Parasitologi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author

Abstract


The study evaluates in vitro effects of Areca catechu crude aqueous extract  as anthelmintics on Ascaridia galli adult mortality rate and morphometry. Naturally infected chickens were collected from slaughtered chickens at local slaughterhouses in Yogyakarta. Chicken’s intestines were carefully examined and transported to the Parasitological Laboratory of Gadjah Mada University, Yogyakarta. Ascaridia galli were collected from intestines (including duodenum, jejunum, and ileum) and put into a petri dish containing 0.62% saline water. There were 60 A. galli in each concentration of Areca catechu crude aqueous extract. Investigation on mortality rate of adult worms at various concentration and observation as well as differences in A. galli morphometry and mortality rate were analyzed using analysis Anova. The figures of the parasites or the parts of the parasites were captured using camera Lucida and then they were measured using both micrometer and curvimeter. The morphology of the parasites was identified to find out their morphometric characteristics.The results of morphometric observation of the A. galli showed that there was a significant difference in the body width and the vulva length of the females and in the body width, the esophageal length and the esophageal width of the males.This research disclosed that Areca catechu crude aqueous extract significantly affected the mortality rate of adult A.galli at various concentration. This research observed that the best concentration to kill the adult worms was 25% Areca catechu crude aqueous extract.


Keywords


Areca catechu; Ascaridia galli; chicken; crude aqueous extract; morphometry

Full Text:

PDF


References

Alvarez, L.I., Mottier, M.L. and Lanusse, C.E. 2007. Drug transfer into target helminthes parasites. Trends Parasitol., 23: 97-104.

 

Amudhan, M.S., Begum, V.H. and Hebbar, K.B. 2012. A review on phytochemical and pharmacological potential of Areca catechu  L. seed. Int. J. Pharm. Sci. Res., 3: 4151-4157.

 

Arisandi Y. 2008. Khasiat Tanaman Obat. Jakarta: Pustaka Buku Merah

 

Bahuaud, D., Martinez-Ortiz de Montellano, C., Chauveau, S., Prevot, F., Torres-Acosta, F., Fouraste, I., Hoste, H. 2006. Effects of Four Tanniferous Plant Extracts on the in Vitro Exsheathment of Third-Stage Larvae of Parasitic Nematodes. Parasitology. 132:545-54.

 

Dalimartha, S. 2009. Atlas Tumbuhan Obat Indonesia. Jakarta. Penebar Swadaya.

Hassanain, M.A., Rahman, E.H.A. and Khalil, F.A.M. 2009. New scanning electron microscopy look of Ascaridia galli (Schrank 1788) adult worm and its biological control. Res. J. Parasitol., 4: 1-11.

Kuchai, J.A., Ahmad, F., Chishti, M.Z., Tak, H., Ahmad, J.A.S. and Razool, M. 2012. A study on morphol­ogy and morphometry of Haemonchus contortus. Pak. J. Zool., 44(6): 1737-1741.

 

Lalchhandama, K., Roy, B. and Dutta, B.K. 2009. Anthelmintic activity of Acacia oxyphylla stem bark against Ascaridia galli. Pharm. Biol., 47(7): 578-583.

 

Lorent, J.H., Quetin-Leclercq, J. and Mingeot-Leclercq, M.P. 2014. The amphiphilic nature of saponins and their effects on artificial and biological membranes and potential consequences for red blood and cancer cells. Org. Biomol. Chem., 12: 8803-8822.

 

Martinez-Ortiz-de-Montellano, C., Arroyo-Lopez, C., Fourquaux, I., Torres-Acosta, J.F.J., Sandoval-Castro, C.A. and Hoste, H. 2013. Scanning electron microscopy of Haemonchus contortus exposed to tannin-rich plants under in vivo and in vitro conditions. Exp. Parasitol., 133(3): 281-286.

 

Max, R.A., Dawson, J.M., Wakelin, D., Buttery, P.J., Kimambo, A.E., Kassuku, A.A. dan Mtenga, L.A. 2002. Effect of Condensed Tanin Extracts on Gastrointestinal Nematodes of Small Ruminants. In: Proceedings of the Second Dfid Livestock Production Programme Link Project (R7798) Workshop for Smallstock Holders. Sokoine University of Agriculture, Morogoro, Tanzania

 

Nyambuya, T., Mautsa, R. and Mukanganyama, S. 2017. Alkaloid extracts from Combretum zeyheri inhibit the growth of Mycobacterium smegmatis. BMC Compl. Altern. Med., 17(124): 1-11.

 

Oliveira-Menezes, A., Lins, R., Norões, J., Dreyer, G. and Lanfredi, R.M. 2007. Comparative analysis of a chemotherapy effecton the cuticular surface of Wuchereria bancrofti adult worms in vivo. Parasitol. Res., 101: 1311-1317.

 

Ramadan, H. H. and Znada, N. Y. A. 1992. Morphologi and life history of Ascaridia galli in the domestic fowl that are raised in Jeddah. J.K.A.U.Sci., 4: 87- 99.

 

Robinson, M.W., McFerran, N., Trudgent, A., Hoey, L. and Fairweather, I. 2004. A possible model of benzimidazole binding to ß-tubulin disclosed by invoking an inter-domain movement. J. Mol. Graph. Model., 23: 275-284.

 

Roy, B., Dasgupta, S., Manivel, V., Parameswaran, P.S. and Giri, B.R. 2012. Surface topographical and ultrastructural alterations of Raillietina echinobothrida and Ascaridia galli induced by a compound isolated from Acacia oxyphylla. Vet. Parasitol., 185: 322-326.

 

Sambodo, P., Prastowo, J., Kurniasih, K. and Indarjulianto, S. 2018. In vitro potential anthelmintic activity of Biophytum petersianum on Haemonchus contortus. Vet. World., 11(1): 1-4.

 

Sandika, B., Raharjo & Ducha, N. 2012. Pengaruh Pemberian Air Rebusan Akar Delima (Punica granatum L) Terhadap Mortalitas Ascaris suum. Goesze. Secara In Vitro. Lentera Bio, 1:81-86.

Shahidi, F and M. Naczk. 1995. Food Phenolics. Technomic Inc, Basel. p.481-482

Tiwow, D., Bodhi W. & Kojong, N.S. 2013. Uji Efek Antelmintik Ekstrak Etanol Biji Pinang (Areca catechu) Terhadap Ascaris lumbricoides dan Ascaridia galli Secara In Vitro. Pharmacon, 76-80.

Waller, P.J. 1999. International Approaches to the Concept of Integrated Control of Nematode Parasites of Livestock. Int. J. Parasitol. 29,155–164.

 

Widiarso, B.P., Kurniasih, K., Prastowo, J., Nurcahyo, W. 2018. Morphology and morphometry of Haemonchus contortus exposed to Gigantochloa apus crude aqueous extract. Vet. World., 11(7): 921-925.

 

Zalizar L, Rahayu I.D. 2001. Pengaruh penggunaan larutan bawang putih terhadap penampilan produksi ayam lurik penderita parasit cacing. Jurnal Agritek, 9:2.

 

Zalizar L, Satrija F, Tiuria R, Astuti DA. 2006. Dampak infeksi Ascaridia galli terhadap gambaran histopatologi dan luas permukaan vili usus serta penurunan bobot hidup starter. Jurnal Ilmu Ternak dan Veteriner 11(3): 215-222.

 

Zalizar L, Fadjar. S, Risa. T, Dewi AA. 2007. Respon ayam yang mempunyai pengalaman infeksi ascaridia galli terhadap infeksi ulang dan implikasinya terhadap produktivitas dan kualitas telur. Animal Production. Jurnal Produksi Ternak 9(2): 92-98.

 

Zhong, R.Z., Sun, H.X., Liu, H.W. and Zhou, D.W. 2014. Effects of tannin acid on Haemonchus contortus larvae viability and immune responses of sheep white blood cells in vitro. Parasite Immunol., 36: 100-106.



DOI: https://doi.org/10.22146/jsv.43751

Article Metrics

Abstract views : 207 | views : 190

Refbacks

  • There are currently no refbacks.




Copyright (c) 2019 Jurnal Sain Veteriner

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Sain Veteriner Indexed by

    CrossrefROADCOREDimensions  JournalTOCs   


Copyright of JSV (Jurnal Sain Veteriner) ISSN 0126-0421 (print), ISSN 2407-3733 (online).

Fakultas Kedokteran Hewan, Universitas Gadjah Mada

Jl. Fauna No.2, Karangmalang, Yogyakarta

Phone: 0274-560862

Fax: 0274-560861

Email: jsv_fkh@ugm.ac.id

Jurnal Sain Veteriner is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 

free
web stats View My Stats