Pelacakan eskpresi protein pada testis mencit (Mus musculus) setelah paparan esktrak etanol daun mimba (Azadirachta indica)

https://doi.org/10.22146/jsv.43027

Agung Janika Sitasiwi(1*), Sri Isdadiyanto(2), Siti Muflichatun Mardiati(3)

(1) Departemen Biologi Kampus UNDIP Tembalang UNDIP Semarang
(2) Departemen Biologi Kampus UNDIP Tembalang UNDIP Semarang
(3) Departemen Biologi Kampus UNDIP Tembalang UNDIP Semarang
(*) Corresponding Author

Abstract


Abstract

 

            Azadirachta indica (Neem) has been shown to affect the fertility of mice by interfering with the synthesis of testosterone in mice. The aim of this study was to detect the testes protein expression of mice after exposure to the ethanolic Neem leaf extract. The laboratory animals of this study were 20 male Swiss Webster mice with three months in age and body weight ranging from 27.5 grams. The mice were divided into two treatment groups, namely K (control group, exposed with distilled water) and P (treatment group, exposed to etahnolic Neem leaf  extract with 14 mg/animal /day). The treated were given for 21 days and the testicular protein was carried out on the 22nd day. The variables observed were testes weight, concentration and expression of proteins isolated from the testes. The protein concentration is determined by a spectrophotometer at a wavelength of 450nm. The protein expression was observed and determined based on the results of protein electrophoresis (SDS-PAGE). The results showed that protein expression in the treatment group has a lower concentration compared to the control group. Those results  was confirmed by thinner bands in SDS-PAGE result. Those proteins thought to be a fertility determinant in mammals.

 

Keywords : anti-fertility; Neem; protein expression

 

Abstrak

 

Azadirachta indica (Mimba) telah terbukti mempengaruhi fertilitas mencit dengan cara mengganggu sintesis hormon testoteron pada mencit. Penelitian ini bertujuan untuk melacak ekspresi protein pada testis mencit setelah paparan ekstrak etanol daun Mimba. Hewan uji penelitian ini adalah 20 ekor mencit Swiss Webster jantan dengan umur tiga bulan dan bobot badan berkisar 27.5 gram. Hewan uji dibagi menjadi 2 kelompok perlakuan, yaitu K (kelompok kontrol, dipapar akuades) dan  P (kelompok perlakuan, dipapar dengan ekstrak etanol daun Mimba dengan dosis 14 mg/ekor/hari). Pemberian bahan uji dilakukan  secara oral selama 21 hari. Variabel yang diamati adalah bobot testes, konsentrasi serta eskpresi protein yang diisolasi dari testis. Isolasi protein testis dilakukan pada hari ke-22. Konsentrasi protein ditentukan dengan spectrofotometer pada panjang gelombang 450nm. Ekspresi protein diamati dan ditentukan berdasar hasil elektroforesa protein. Hasil penelitian menunjukkan bahwa ekspresi protein pada kelompok perlakuan menunjukkan konsentrasi yang lebih rendah dengan pita yang lebih tipis jika dibandingkan dengan kelompok kontrol. Kesimpulan penelitian ini adalah paparan ekstrak etanol daun Nimba menyebabkan gangguan ekspresi protein yang diduga berperan dalam menentukan fertilitas mamalia.

 

Kata kunci : Mimba; anti-fertilitas; eskpresi protein;

 

 

 


Keywords


anti-fertility; Neem; protein expression

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References

Asif, M. (2013). A review on Spermicidal activities of Azadirachta indica. Journal of Pharmacognosy and Phytochemistry. 1(5): 61-80.

Auta, T. dan Hasan, A.T. (2016). Reproductive toxicity of aqueous wood-ash extract of Azadirachta indica (neem) on male albino mice. Asian Pacific Journal of Reproduction x(x):1- 5.

Bansal, P., Bansal, R., Gupta, V. (2010). Antifertility effects of Azadirachta indica (Neem) - A Review. Annals of Biological Research. 1(2): 108-113.

Brenner, L.Z., Roselli, C.E., Recabarren, S.E., Gonzalez Deniselle, M.C. and Lara, H.E. (201). Developmental and Functional Effects of Steroid Hormones on the Neuroendocrine Axis and Spinal Cord. J Neuroendocrinol. 28(7).

Creasy, D., Bube, A., de Rijk, E., Kandori, H., Kuwahara, M., Masson, R., Nolte, T., Reams, R., Regan, K., Rehm, S., Rogerson, P. and Whitney, K. (2012). Proliferative and Nonproliferative Lesions of the Rat and Mouse Male Reproductive System. Toxicologic Pathology, 40: 40S-121S.

Dabhadkar, D.K., Thakare, V.G., Zade, V.Z., Charjan, A.P., Dhore, M.M., and Deosthale, S.M. (2015). Review on some ethnobotanical plants having antifertility activity in female albino rats. Int. Res. J. of Science and Engineering. 3(2): 43-46.

Djureinovic, D., Fagerberg, L., Hallstrom, B., Danielsson, A., Lindskog, C., Uhlen, M. and Ponten, F. (2014). The human testis-specific proteome define by transcriptomics and antibody-based profiling. Hum.Reprod. 20(6):476-88.

Francis, G., Kerem, Z., Makkar, H.P.S., Becker, K. (2002). The Biological action of saponins in animal system: review. British Journal Of Nutrition. 88:587-605.

Jan, S.Z., G. Hamer, G., Repping, S., de Rooij, D.G., van Pelt, A.M.M., and Vormer, T.L. (2012). Review. Molecular control of rodent spermatogenesis. Biochemica et Biophysica Acta. 1822: 1838-1850.

Kupradinun, P., Tepsuwan, A., Tanthasri, N., Meesiripan, N., Tunsakul, S., Tompat, W., Jarratwisautpom, Y., and Kusamran, W.R. (2012). Toxicity Testing of Flowers of Neem Tree (Azadirachta indica A. Juss). Thai J. Vet. Med. 40(1): 47-55.

Lee, B. S. Jin, Choi, H., Kwon, J.T., Kim, J., Jeong, J., Kwon, Y. and Cho, C. (2013). Expression and function of the testis-predominan protein LYAR in mice. Mol.Cells. 35(1): 54-60.

Monteiro, J.C., da Matta, S.L.P., Predes, F.S., de Paula, T.A.R. (2012). Testicular Morphology of Adult Wistar Rats Treated with Rudgea viburnoides (Cham.) Benth. Leaf Infusion. Braz. Arch. Biol. Technol. 55(1): 101-105.

Pang, A.L.Y. and Rennert, O.M. (2018). Protein acetylation and spermatogenesis. Reprod. Syst. Sex. Disord. Suppl 1:5_.

Pankaj, S., Lokeswar, T., Mukesh, B. and Vishnu, B. (2011). Review on Neem (Azadirachta indica): Thousand problem one solution. IRJP. 2(12): 97-102.

Sharma, R.K., Goyal, A.K. and Bhat, R.A. (2013). Antifertility of Plants Extracts on Female Reproduction: A Review. IJBS. 3(3): 493-514.

Sitasiwi, A.J., Artama, W.T., Budiyanto, A., dan Dharmana, E. (2016). Pelacakan Protein Wnt4 pada Uterus Mencit Swiss Webster. Jurnal Veteriner UNUD. Vol.17(1): 22-29.

Sitasiwi, A.J., Isdadiyanto, S., Mardiati, S.M. (2017). The Estradiol 17-β Concentration in Mice after Treated with Ethanolic Leaf Extract of Azadirachta indica (Neem). AIP Conference Proceeding. ISBN: 978-0-7354-1516-4.

Suryawanshi, J. A. S. (2011). Neem-natural contraceptive for male and female–an overview. IJBB. 1(2): 1-6.

Tirado, O.M., Martinez, E.D., Rodriguez, O.C., Danielsen, M., Selva, D.M., Reventos, J., Munell, F., Suarez-Quian, C.A. (2003). Methoxyacetic Acid Disregulation of Androgen Receptor and Androgen-Binding Protein Expression in Adult Rat Testis. Biology of Reproduction, Volume 68. Issue 4: 1437–1446.

Umadevi, K., Sampath Khumar, P.K., Bhownik, D., and Duraivel, S. (2013). Medicinal Plants with Potential Antifertility Activities. Journal of Medicinal Plants Studies. 1(1): 26-33.



DOI: https://doi.org/10.22146/jsv.43027

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