Distribusi neuron serotonergik pada hipokampus Lasiwen (Myotis sp) sebagai satwa liar yang berpotensi menjadi reservoir virus rabies


Tri Wahyu Pangestiningsih(1*), Ariana Ariana(2), Irma Padeta(3), Arvendi Rahma Jadi(4), Woro Danur Wendo(5)

(1) Universitas Gadjah Mada
(2) Universitas Gadjah Mada
(3) Universitas Gadjah Mada
(4) Universitas Gadjah Mada
(5) Universitas Gadjah Mada
(*) Corresponding Author


Myotis sp is an Indonesian microchiropteran, insectivore bat that potential to be a reservoir for rabies virus. Rabies is fatal viral encephalitis and  bat could  act as wildlife potential reservoirs for the rabies virus and can transmit the disease to humans as the zoonotic disease. Hippocampus  is one of diagnostic tissue for rabies disease and serotonergic neuron could involved in pathogenesis of rabies disease.  The aim of the study is to investigate the distribution of serotonergic neurons in  Myotis sp hippocampus. Five Myotis sp. were captured from wild population in Central Java, and were humanly anesthetized using ketamine and xylazin. The animals were perfused intracardially using NaCl 0.9% as the pre-rinse followed by 10% formaldehyde to fix it. The cerebrums were  collected and processing the  for paraffin embedding. Cerebrums were sectioning in saggital sections,  12 µm thickness serially with 120 µm intervals.    The tissues  were staining immunohistochemistry using antibody to serotonin (1/300; Bioss, Cat. No: bs-1126R) 2 night incubation in 4 oC temperature. The solution for blocking background, secondary antibody, avidin-biotin-peroxidae complex and chromogen using  kit Starr Trek Universal HRP Detection System (Biocare Medical, Cat No: STUHRP700) and were analyzed descriptivelly. The results show that serotonergic neuros were distributed in the all area of  the of Myotis sp hippocampus. In  dentate gyrus neuron serotonergic (Sert-IR)s are round in shape and  mostly distributed in the middle layer, few in the  superficially also deeper layers. In the hippocampus , the Sert-IR neurons are pyramidal in shape and distributed in the CA1, Ca2 and CA3 areas. In subiculum, the Sert-IR neurons  are pyramidal in shape, more wider distributed than in the CA1 with no differences between outer layer and deeper layer.  The conclutions of this research are the serotonergic neurons are distributed in the all area of hippocampus


hippocampus; Myotis sp; serotonergic neurons.

Full Text:



Altringham, J.D. 1996.Bats.Biology and Behavior.Oxford University press. New York

Banyard, A.C., Hayman, Johnson, N., McElhinney, L., dan Ffook, A.R. 2011. Research Advances in Rabies. Chapter 12- Bat and Lyysaviruses. Advances in Virus Research. 79: 239-289.

Calvey, T., Patzke, N., Kaswera- Kyamakya, C., Gilissen, E., Bertelsen. M.F., Pettigrew, J.D., and Manger , P.R. 2015. Organization of cholinergic, catecholaminergic, seroto nergic and orexinergic nuclei in three strepsirrhine primates: Galago demidoff, Perodicticus potto and Lemur catta. J. Chem. Neuroanat. 70, 42–57

Corbet, G. B., dan J. E. Hill. 1992. Mammals of the Indomalayan region: a systematic review. Oxford University Press, New York.

Johnson, N., Vos, A., Freuling, C., Tordo, N.,Fooks, A.R., dan Mu¨ller, T. 2010. Review: Human rabies due to lyssavirus infection of bat origin.Vet Microbiol 142, 151–159

Kruger, J-L., Dell, L-A.Bhagwandin,A., Jillani, N.E., Pettigrew, J.D., dan Manger, P.R. 2010. Nuclear organization of cholinergic, putative catecholaminergic and serotonergic systems in the brains of five microchiropteran species. J Chem Neuroanatomy. 40, 210-222

Maseko, B.C., Bourne, J.A., and Manger, P.R., 2007. Distribution and morphology of cholinergic, putative catecholaminergic and serotonergic neurons in the brain of the Egyptian Rousette flying fox,Rousettus aegyptiacus. J. Chem. Neuroanat. 34, 108 –127.

Maseko, B.C., dan Manger, P.R., 2007. Distribution and morphology of cholinergic,

catecholaminergic and serotonergic neurons in the brain of Schreiber’s longfingered bat, Miniopterus schreibersii. J. Chem. Neuro- anat. 34, 80–94.

Nouvo,G.J., DeFaria,D.L., Ghanona,J.G., dan Zhang,Y.2005. Molecular Detection of Rabies Encephalitis and Correlation with Cytokine Expression. Modern Pathology 18,62-67.

Pangestiningsih, T.W., Ariana, Wendo, W.D. Dan Wijayanto, H. 2017. Histological Features Of Hippocampal Microchiropteran Bat (Myotis Sp.) As A Potential Wildlife Animal Reservoir For Rabies Virus. Seminar internasional di 6th Congress of Asian Association of Veterinary Anatomists. Kuching, The Sarawak, Malaysia. October 15-16, 2017

Patzke, N., Bertelsen, M.F. , Fuxe, K. , dan Manger , P.R. 2014. Nuclear organization of cholinergic, catecholaminergic, serotonergic and orexinergic systems in the brain of the Tasmanian devil (Sarcophilus harrisii). J. Chem. Neuroanat. 61-62, 94–106

Pieters, R.P., Gravett, N., Fuxe, K., and Manger, P.R., 2010. Nuclear organization of

cholinergic, putative catecholaminergic and serotonergic nuclei in the brain

of the eastern rock elephant shrew, Elephantulus myurus. J. Chem. Neuroanat.

, 175–18

Pillaya, S., Bhagwandina, A., , Bertelsenb, M.F., Patzkea, N., Englerc, G., Engelc, A.K, and Manger, P.R. 2017. Regional distribution of cholinergic, catecholamine- ergic, serotonergic and orexinergic neurons in the brain of two carnivore species: The feliform banded mongoose (Mungos mungo) and the caniform domestic ferret (Mustela putorius furo). J. Chem. Neuroanat 82,12-28

Imleda, Y.M. dan Sudewi, A.A. 2015. Patogenesis Rabies- Aspek Neurotransmiter. CDK-225. 42 (2): 87-91

Wicaksono, B.S. 2018. Kemenkes: Banyak anak meninggal karena rabies. Kompas.com. 24-08-2018. https://sains.kompas.com. [Akses 23 Okoteber 2018]

DOI: https://doi.org/10.22146/jsv.42914

Article Metrics

Abstract views : 2566 | views : 2922


  • There are currently no refbacks.

Copyright (c) 2019 Jurnal Sain Veteriner

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Sain Veteriner Indexed by


Copyright of JSV (Jurnal Sain Veteriner) ISSN 0126-0421 (print), ISSN 2407-3733 (online).

Fakultas Kedokteran Hewan, Universitas Gadjah Mada

Jl. Fauna No.2, Karangmalang, Yogyakarta

Phone: 0274-560862

Fax: 0274-560861

Email: jsv_fkh@ugm.ac.id

HP. 0895363078367

Jurnal Sain Veteriner is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.


web stats View My Stats