Kajian Kliniko-patologik dan Antimikroba Ekstrak Biji Alpukat (Persea Americana Mill)
Christin Marganingsih Santosa(1*), Imron Rosyadi(2), Dinar Arifianto(3), Salasia Isrina Oktavia Siti(4)
(1) Departemen Patologi Klinik Fakultas Kedokteran Hewan Universitas Gadjah Mada, Yogyakarta
(2) Departemen Patologi Klinik Fakultas Kedokteran Hewan Universitas Gadjah Mada, Yogyakarta
(3) Departemen Patologi Klinik Fakultas Kedokteran Hewan Universitas Gadjah Mada, Yogyakarta
(4) Departemen Patologi Klinik Fakultas Kedokteran Hewan Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author
Abstract
The phytochemical components of avocado seeds (Persea americana Mill) consist of alkaloids, flavonoids, tannins and saponins, so that they have the potential as antibacterials. The aim of this study was to determine the ability of Persea americana Mill extract to inhibit the growth of Staphylococcus aureus through inhibition zone observation and the ability to accelerate wound healing infected by S. aureus through observation of wound closure and cytopathology. Persea americana Mill were extracted using maceration method with 70% ethanol. The activities of this extract were tested by diffusion disc agar method. Biomedical tests were carried out on anaestetic Wistar rats whose were S. aureus infected wounds and treated with Persea Americana Mill extract. Based on the results of the antimicrobial test, avocado seed extract has a inhibitory effect on the growth of S. aureus at a concentration of 6.25% and best at a concentration of 100% compared to the control. Tests in experimental rats showed the effect of wound healing on the 2rd day after giving the extract Persea americana Mill ointment, faster than the control, which was need 4 days of wound closure. Cytopathology results showed that leukocyte activity was more prominent in rats treated with avocado seed extract compared to control rats. Avocado seeds as byproducts have the potential to be new herbal medicines that can be used as antimicrobials of S. aureus which have been confirmed to be resistant to various antibiotics.
Keywords
Full Text:
PDFReferences
Balouiri, M., Sadiki, M., Ibn souda, S.K. 2017. Methods for in vitro evaluating antimicrobial activity: A review. J of Pharmaceutical Analysis 6: 71 – 79.
Carpenter, 2013. Exotic Animal Formulary. 4th edition. Elsevier Inc. Missouri.
Chia, T.W.R., & Dykes, G.A., 2010. Antimicrobial Activity of Crude Epicarp and Seed Extracts from Mature Avocado Fruit (Persea americana) of Three Cultivars. Pharmaceutical Biology, 48(7): 753-756.
Hando, E.K. 2017. Efek Rebusan Daun Alpukat (Persea Americana Mill.) Terhadap Kemampuan Adhesi Bakteri Streptococcus sanguinis ATCC10556 in-Vitro. Skripsi. Fakultas Kedokteran Gigi. Universitas Gadjah Mada, Yogyakarta. Indonesia.
Leite, J.J.G., Brito, E.H.S., Cordeiro, R.A., Brilhante, R.S.N., Sidrim, J.J.C., Bertini, L.M., Morais, S.M.D., Rocha, M.F.G. 2009. Chemical Composition, Toxicity and Larvacidal and Antifungal Activities of Persea americana (Avocado) Seed Extract. Revista da Sociedadae Brasileira de Medicina Tropical. 2(42): 110-113
Marlinda, M., Sangia, M.S., Wuntua, A.D. 2012. Analisis Senyawa Metabolit Sekunder dan Uji Toksisitas Ekstrak Etanol Biji Buah Alpukat (Persea americana Mill.). diakses melalui
http://ejournal.unsrat.ac.id/index.php/jmuo , 5 Maret 2018.
Salasia, S. I. O. and Khusnan (2011). Characteristics of Staphylococcus aureus isolates in Indonesia. The International Conference on Natural Sciences (ICONS), Humbold Kollege. Malang, July 9-11
Samsiati, E.H. 2016. Penentuan Aktivitas dan Identifikasi Senyawa Aktif Antioksidan Dalam Biji Buah Alpukat. Tesis Post graduate. Fakultas Farmasi. Universitas Gadjah Mada, Yogyakarta. Indonesia.
Valgas, C., Souza, S.M.d., Smânia, E.F., & Smânia Jr, A. 2007. Screening methods to determine antibacterial activity of natural products. Brazilian Journal of Microbiology, 38(2), 369-380.
Widiyastuti, Y. 2017. Kajian Senyawa Berpotensi Antikanker Biji Alpukat (Persea Americana Mill.): Isolasi Senyawa Sitotoksik dan Mekanisme Kerja Terhadap Sel Kanker MCF-7. Disertasi. Fakultas Farmasi. Universitas Gadjah Mada, Yogyakarta. Indonesia.
DOI: https://doi.org/10.22146/jsv.40445
Article Metrics
Abstract views : 2512 | views : 3522Refbacks
- There are currently no refbacks.
Copyright (c) 2019 Jurnal Sain Veteriner
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Sain Veteriner Indexed by
Copyright of JSV (Jurnal Sain Veteriner) ISSN 0126-0421 (print), ISSN 2407-3733 (online).
Fakultas Kedokteran Hewan, Universitas Gadjah Mada
Jl. Fauna No.2, Karangmalang, Yogyakarta
Phone: 0274-560862
Fax: 0274-560861
Email: jsv_fkh@ugm.ac.id
HP. 0895363078367
Jurnal Sain Veteriner is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
View My Stats