Bacterial Detection Causing Subclinical Mastitis on Etawah Crossbreed Goat in Kokap, Kulon Progo, Yogyakarta Province

Clara Ajeng Artdita(1*), Morsid Andityas(2), Nur Ika Prihanani(3), Yohanes Wawan Budiyanto(4)

(1) Veterinary Paramedic Study Program Vocational College Universitas Gadjah Mada
(2) Veterinary Paramedic Study Program Vocational College Universitas Gadjah Mada
(3) Veterinary Paramedic Study Program Vocational College Universitas Gadjah Mada
(4) Veterinary Paramedic Study Program Vocational College Universitas Gadjah Mada
(*) Corresponding Author


Resident in Kokap, Kulon Progo, Yogyakarta Province has another income sector, that is dairy goat (Etawah Crossbreed) farming and they have been joining into farm group, which is called Kelompok Ternak. There are many bacteria that causing dairy goat intra mamary infection, thus the milk production will be contaminated. Two hundred and four (204) milk samples from 103 lactation goat in four farm groups in Kokap, Kulon Progo, Yogyakarta is used in this study. Out of these, 21 samples (10,29%) were detected strong positive (++ and +++) for subclinical mastitis by using California Mastitis Test (CMT). Among this positive result, the next step was bacteria detection. This tests were held in Preclinic Laboratory Animal Health Study Program, Vocational School, UGM. The samples were inoculated in blood agar, then colony was identify by Gram staining. The next steph is biochemical tests using McConkey Agar (MCA) and eosin methylene blue (EMB) as a selective media for Gram negative bacteria,  manitol salt agar (MSA) as selective media for Staphylococcus, sugar fermented broth (glucose, lactose, mannitol, maltose, and sacharose), Kigler iron agar (KIA), lysine iron agar (LIA), motility indole ornithin (MIO), and Simmon’s citrate. The result was Staphylococcus aureus (80%), Staphylococcus epidermidis (20%), Escherichia coli (10%), and Klebsiella pneumoniae (10%).



Bacterial detection; Etawah crossbreed goat; Kokap area; Subclinical Mastitis

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Bannerman, D.D., Paape, M.J., Hare, W.R., dan Sohn EJ. (2003). Increased levels of LPS-binding protein in bovine blood and milk following bacterial lipopolysaccharide challenge. J. Dairy Sci. 86,:3128-3137. Ceballos, L.S., Morales, E.R., Adarve, G.D.L.T., Castro, J.D., Martinez, L.P., dan Sampelayo, M.R.S. (2009). Composition of goat and cow milk produced under similar conditions and analyzed by identical methodology. J Food Comp Analysis. 22(4) : 322-329. Contreras, A., Luengo, C., Sánchez, A. dan Corrales, J.C. (2003). The role of intramammary pathogens in dairy goats. Livestock Production Science. 79:273–283. Contreras, A., Sierra, D., Sánchez, A., Corrales, J.C., Marco, J.C., Paape, M.J. dan Gonzalo, C. (2007). Mastitis in small ruminants. Small Ruminant Research. 68:145–15. Deinhofer, M. dan Pernthaner, A. (1995). Staphylococcus spp. as mastitis-related pathogens in goat milk. Vet. Microbiol. 43:161–166. Dinas Pertanian DIY. (2018). Laporan Kinerja Instansi Pemerintah tahun 2017. P: 38. Forbes, B.A., Shamn, D.F., dan Weisffeld, A.S. (2002). Bailey and Scott’s Diagnostic Microbiology. Twelveth edition. Pp: 222-224, 226, 232-246. USA, Mosby. Grohn, Y.T., Wilson, D.J., Gonzalez, R.N., Hertl, J.A., Schulte, H., Bennet, G., and Schukken, Y.H. (2004). Effect of pathogen-specific clinical mastitis on milk yield in dairy cows. J Dairy Sci. 87: 3358–3374. Guha, A., Guha, R., and Gera, S. (2012). Comparison of somatic cell count, California mastitis test, chloride test and rennet coagulation time with bacterial culture examination to detect subclinical mastitis in riverine buffalo (Babalus bubalis). African Jjournal of Agricultureal Research. 7(41):5578-5584. Hall, S.M., and Rycroft, A.N. (2007). Causative organisms and somatic cell counts in subclinical intramammary infections in milking goats in the UK. Vet Rec.160 (1) : 19-22. Leitner, G., Merin, U., Silanikove, N., Ezra, E., Chaffer, M., Gollop, N., Winkler, M., Glickman, A., dan Saran, A. (2004). Effect of subclinical intramammaryinfection on somatic cell counts, NAGase activity and gross composition of goats milk. J. Dairy Res. 71:311-315. Luengo, C., Sanchez, A., Corrales, J.C., Fernadez, C., dan Contreras, A. (2004). Influence of intramammary infection and non-infection factors on somatic cell counts in dairy goats. J. Dairy Res. 71:169-174. Marogna, G., Pilo, C., Vidili, A., Tola, S., Schianchi, G., dan Leori, S.G. (2012). Comparison of clinical findings, microbiological result, and farming parameters in goat herds affected by recurrent infectious mastitis. Small Rumin Res. 102:74-83 McDougall, S., Pankey, W., Delaney, C., Barlow, J., Murdough, P.A., and Scruton, D. (2002). Prevalence and incidence of subclinical mastitis in goats and dairy ewes in Vermont USA. Small Rumin Res. 46(2-3) : 115-121. Mørk, T., Kvitle, B., Mathisen, T., dan Jørgensen, H. J. (2010). Bacteriological and molecular investigations of Staphylococcus aureus in dairy goats. Veterinary microbiology, 141(1), 134-141. Moeljanto, R.D. dan Wiryanta, B.T.W. (2002). Efficacy and Benefits of Goat Milk: the Best Milk of Ruminant. Agro Media Pustaka: Jakarta. Moroni, P., Pisoni, G., Vimercati, C., Rinaldi, M., Castiglioni, B., Cremonesi, P., dan Boettcher, P. (2005). Characterization of Staphylococcus aureus Isolated from Chronically Infected Dairy Goats. Journal of dairy science, 88(10), 3500-3509. Munoz, M.A., Bennett, G.J., Ahlström, C., Griffiths, H.M., Schukken, Y.H., dan Zadoks, R.N. (2008). Cleanliness scores as indicator of Klebsiella exposure in dairy cows. J Dairy Sci. 91(10):3908 –16. National Mastitis Council (US). (1999). Laboratory Handbook on Bovine Mastitis. WD Hoard & Son. p:222. Paape, M.J, dan Capuco, A.V. (1997). Cellular Defense Mechanisms in the Udder and Lactation of Goats. J Anim Sci. 75(2) : 556-565. Persson, Y., dan Olofsson, I. (2011). Direct and indirect measurement of somatic cell count as indicator of intramammary infection in dairy goats. Acta Vet Scand. 53(15) : 1-5. Quinn, P.J., Carter, M.E., Markey, B., dan Carter, G.R. (2004). Clinical Veterinary Microbiology. Pp: 209-221. USA, Mosby. Seegers, H., Fourichon, C., dan Beaudeau, F. (2003). Production effects related to mastitis and mastitis economics in dairy cattle herds. J. Vet. Res. 34(5):475-491. Sirois, M. (2015). Laboratory Procedures for Veterinary Technicians. Sixth edition. Pp:2587-261. Missouri, Elsevier Mosby. Subronto. (2007). Ilmu Penyakit Ternak II (Mamalia). Yogyakarta: Gadjah Mada University Press. Pp: 320-332. Todar, K. (2008). Staphylococcus aureus and Staphylococcal Disease; Todar's Online Textbook of Bacteriology. www.textbook of Wooding, F.B.P., Peaker, M. dan Linzell, JL. (1970). Theories of milk secretion: Evidence from the electron microscopic examination of milk. Nature (Lond.) 226:762. Yangilar, F. (2013). As a potentially functional food : goat’s milk and products. Journal of food and nutrition research. 1(4):68-81. Zadoks, R.N., Middleton, J.R., McDougall, S., Katholm, J., dan Schukken, Y.H. (2011). Molecular epidemiology of mastitis pathogens of dairy cattle and comparative relevance to humans. J Mammary Gland Biol Neoplasia. 16: 357–372. Zhao, Y., Liu, H., Zhao, X., Gao, Y., Zhang, M., dan Chen, D. (2015). Prevalence and pathogens of subclinical mastitis in dairy goats in China. Trop Anim Health Prod. 47:429–435.


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