Immunodiagnosis Infeksi Aeromonas hydrophila pada Ikan

https://doi.org/10.22146/jsv.38858

Yuli Purwandari Kristianingrum(1*), Sitarina Widyarini(2), Kurniasih Kurniasih(3), Bambang Sutrisno(4), Charles Rangga Tabbu(5), Sugiyono Sugiyono(6)

(1) Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Yogyakarta
(2) Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Yogyakarta
(3) Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Yogyakarta
(4) Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Yogyakarta
(5) Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Yogyakarta
(6) Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author

Abstract


Aeromonas hydrophila causes a disease that often infects fish and is known as Motile Aeromonas Septicaemia (MAS), Hemorrhagi Septisemia, Ulcer disease or Red-Sore disease. The   aims of this study were to develop polyclonal antibody of  Aeromonas hydrophila in the rabbits to   confirm the diagnosis of Aeromonas hydrophila  in the fish by immunohistochemistry staining method. Preparation of polyclonal antibodies was performed on the rabbits used to Aeromonas hydrophila bacteria that have been tested biochemically by intravenous and intraperitoneal injection. Doses of Aeromonas hydrophila  bacteria were 109 CPU/ml  of 0.5 ml at first injection, 1 ml at second injection, 2 ml at thirth injection and 3 ml at fourth injection. Blood serum collection was performed at week 5 after injection from  an  ear and intracardial vein. The result of antibody titer was 28 = 1024 which measured by   tube test. Furthermore, polyclonal   antibody was used to immunohistochemistry  staining with 400x dilution. The results of the staining showed that an immunopositive reaction in the liver, skin,lien,  gill, kidney, and heart of fish to Aeromonas hydrophila antibody. The research conclution was polyclonal antibody from rabbit can be used to accurately confirm the diagnosis of Aeromonas hydrophila  based on antigen and antibody reaction.

 


Keywords


Aeromonas hydrophila; immunohistochemistry; antigen; antibody

Full Text:

PDF


References

Austin, B., Austin, D. A. (2007) Bacterial Fish Pathogens : Diseases of Farmed and Wild Fish. Fourth Edition. Springer Inc., New York. : 83-84.

 

Bootland, L.M., dan Leong, J.A. (1992) Staphylococcal Coaglutination, a Rapid Method of Identifying Infectious Hematopoetic Necrosis Virus. Appl. and Env. Microbiol. : 6-13

 

Buller, N. B. (2014) Bacteria and Fungi from Fish and Other Aquatic Animals : A Practical Identification Manual. Second Edition. CAB International Publishing, Oxfordshire. : 23, 151-152, 458

 

Cartwright, G.A., Chen, D., Hanna, P.J., Gudkovs, N., Tajima, K. (1994) Immunodiagnosis of virulent strains of Aeromonas hydrophila associated with epizootic ulcerative syndrome (EUS) using monoclonal antibody. J. Fish Disease. 17: 123-133

 

Coscelli, G.A., Bermudez, R., Sancho Silva, A.R., Ruiz de Ocenda, M.V., Quiroga, M.I. 2014. Granulomatous dermatitis in turbot (Schopthalmus maximus L.) associated with natural Aeromonas salmonicida subps.salmonicida infection. Aquaculture 428: 111-116

 

Coscelli, G.A., Bermudez, R., Losada, A.P., Failde, L.D., Santos, Y., Quiroga, M.I. (2014) Acut Aeromonas salmonicida infection (Schopthalmus maximus L.). Histopathological and immunohistochemical studies. Aquaculture 430: 79-85

 

Deen, A. E. N. E., Dorgham, S. M., Hassan, A. H. M., Hakim, A. S. (2014) Studies on Aeromonas hydrophila in Cultured Oreochromis niloticus at Kafr El Sheikh Governorate, Egypt with Reference to Histopathological Alterations in Some Vital Organs. World Journal of Fish and Marine Sciences 6(3): 233-240.

 

Dosim., Hardi, E. H., Agustina. (2006) Efek Penginjeksian Produk Intraseluler (ICP) dan Ekstraseluler (ECP) Bakteri Pseudomoas sp. terhadap Gambaran Darah Ikan Nila (Oreochromis niloticus). Jurnal Ilmu Perikanan Tropis, 19(1): 1-7.

 

FAO (Food and Agriculture Organization of the United Nations). (2009)  FAO          Yearbook         :          Fishery            and      AquacultureStatistics. http://www.fao.org/fishery/ (Diakses pada 7 Januari 2017).

 

Farto, R., Milton, D.I..,Bermudez, M., Nieto, T. (2011) Colonization of  turbot tissues by virulent and avirulent Aeromonas salmonicida subsp. salmonicida leucocyte strains during infection. Dis.Aquat.Org. 95:  167-173

 

Irianto, A. (2006) Patologi Ikan Teleostei. Gadjah Mada University Press, Yogyakarta: 144-147.

 

Iskandar, R., Elrifadah. 2015. Pertumbuhan dan Efisiensi Pakan Ikan Nila (Oreochromis niloticus) yang Diberi Pakan Buatan Berbasis Kiambang. Jurnal Medika Veterinaria 40(1). 18-24.

Korbsrisate, S., Dumnin, S., Chawengkirttikul, R., Gherunpong, V., Eampokalap, B., Gongviseisoog, C., Janyapoon, K., Lertpocasombat, K., and Shimada, T. (2002)  Distribution of Aeromonas hydrophila serogroups in different clinical samples and the development of polyclonal antibodies for rapid identification of the genus Aeromonas by direct agglutination. Microbial Immunol. 46: 875-879

 

Lamers C H J, De Hass M J M. (1985)  Antigen localization in the lymphoid organs of carp (Cyprinus carpio) Cell Tissue Res.  242:491–498

 

Merino, S., Rubires, X., Aguilar, A., Tomas,, J.M. (1997)  The role of flagella and motility in the adherence and invasion to fish cell lines by Aeromonas hydrophila serogroup O:34 strains. FEMS Microbiol. Lett. 151: 213-217

 

Moral C.H.,  Castillo E.F., Fierro P.L., Cortés A.V., Castillo J.A., Soriano, A.C.,  Salazar, M.S., Peralta, B.R., and  Carrasco, G.N. (1998) Molecular Characterization of the Aeromonas hydrophila aroA Gene and Potential Use of an Auxotrophic aroA Mutant as a Live Attenuated Vaccine. Infect immun 66(5): 1813-1821

 

Oakey, H.J., Gibson, L.F. and George, A.M. 1999. DNA probe specific for Aeromonas hydropilla (HG1). J Appl. Microbiol., 86: 187-193

 

Rey,  A.,  Verjan,  N.,  Ferguson,  H.  W.,  Iregui,  C.  (2009)  Pathogenesis  of Aeromonas hydrophila Strain KJ99 Infection and its Extracellular Products in Two Species of Fish. The Veterinary Records 164(1): 493-499.

 

Sumithra, T.G., Chaturvedi, V.K., Grupta, P.K., Siju, S.J., Susan, C., Bincy, J.,Laxmi, U.,Sunita, S.C., Rai, A.K. (2014) Development of Simple Method for Rapid Identification of Organism causing Anthrax by Coagglutination Test. Biologicals 42: 316-321

 

Swan, L and White, M.R.  (1989) A Guide to Approved Chemicals in Fish Production and Fishery Resource Management: Diagnosis and Tretment of Aeromonas hydrophila infection of Fish. Aqua Culture Extension. University of Arkansas Cooperative Extension Service.

Woo, P. T. K. (2006) Fish Diseases and Disorders : Protozoan and Metazoan. Volume 1. CABI Publishing, Canada : 163-165, 321-322.

 

Woo, P. T. K., Bruno, D. W. (2011) Fish Diseases and Disorders : Viral Bacterial and Fungal Infection. Volume 3. CABI Publishing, Canada: 424-455.

 

Yardimci,B., Aydin, Y. (2011) Pathological Findings of Experimental Aeromonas hydrophila  Infection  in  Nile  Tilapia  (Oreochromis  niloticus).  Ankara Univ Vet Fak Derg 58(1): 47-54.

 

Yoshimizu, M. dan Kimura, T. (1985) A Coagglutinationtest with antibody sensitized Staphylococci for rapid and simple diagnosis of Bacterial and Viral Diseases of Fish. Fish Pathology, 20 : 243-261

 

Zhang, Y.L.,Ong, C.T., and Leung, K.Y. (2000) Molecular analysis of genetic differences between virulent and avirulent strains of Aeromonas hydropilla isolated from disease fish. Microbiol, 146: 999-1009

 



DOI: https://doi.org/10.22146/jsv.38858

Article Metrics

Abstract views : 593 | views : 486

Refbacks

  • There are currently no refbacks.




Copyright (c) 2018 Jurnal Sain Veteriner

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Sain Veteriner Indexed by

  CrossrefROADCOREDimensions  JournalTOCs   


Copyright of JSV (Jurnal Sain Veteriner) ISSN 0126-0421 (print), ISSN 2407-3733 (online).

Fakultas Kedokteran Hewan, Universitas Gadjah Mada

Jl. Fauna No.2, Karangmalang, Yogyakarta

Phone: 0274-560862

Fax: 0274-560861

Email: jsv_fkh@ugm.ac.id

Jurnal Sain Veteriner is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 

free
web stats View My Stats