The Detection of Encoding Gene of Toxic Shock Syndrome Toxin-1 S. aureus Isolate from the Milk of Cows and Goats by Polymerase Chain Reaction

https://doi.org/10.22146/jsv.3804

Rony M Kunda(1*), A.E.T.H. Wahyuni(2), Rini Widayanti(3)

(1) Alumnus Pascasarjana Sain Veteriner FKH UGM
(2) Bagian Mikrobiologi Fakultas Kedokteran Hewan UGM
(3) Bagian Biokimia Fakultas Kedokteran Hewan UGM
(*) Corresponding Author

Abstract


Abstract

 

Staphylococcus aureus is the main bacterium found in cow's and goat's milk. The bacteria can produce toxin called toxic shock syndrome toxin-1 (TSST-1) that can infect humans and animals causing several serious diseases. The objective of this study was to detect the existence of encoding gene of TSST-1 S. aureus isolate from cow's and goat's milk. The research is initiated by re-identification stage of S. aureus conventionally continued by identification based on the molecular method of polymerase chain reaction (PCR). A number of 10 S. aureus isolates from cow's and goat's milk cultured in aerobics continued by Gram stain, catalase, coagulase, MSA, VJA and VP tests. The identification of S. aureus based on the molecular approach conducted by 16S rRNA gene amplification continued with amplification of TSST-1 encoding gene as the target gene. The PCR product of TSST-1 encoding gene then sequenced to ensure whether the DNA fragment amplified is the TSST-1 encoding gene or not. The result of the research indicates that re-identification of S. aureus conventionally generating positive reaction of S. aureus species. Molecular identification of 16S rRNA gene amplification gives a good result by producing DNA fragment of 745 bp size and meets the target gene. The detection result of encoding gene TSST-1 gives negative result marked by DNA fragment which the size does not match the target gene. The allignment result of sequence isolate SA.1 indicates that the sequence is not tst gene but the gene which coded glutamate sinthetase belongs to S. aureus, whereas sequence of isolate KI.8 is 50S rRNA gene belongs to

S. saprophyticus.


Keywords


Staphylococcus aureus, TSST-1, polymerase chain reaction, cow's and goat's milk, 16S rRNA

Full Text:

PDF



DOI: https://doi.org/10.22146/jsv.3804

Article Metrics

Abstract views : 325 | views : 1075

Refbacks

  • There are currently no refbacks.




Copyright (c) 2014 Jurnal Sain Veteriner

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Sain Veteriner Indexed by

  CrossrefROADCOREDimensions  JournalTOCs   


Copyright of JSV (Jurnal Sain Veteriner) ISSN 0126-0421 (print), ISSN 2407-3733 (online).

Fakultas Kedokteran Hewan, Universitas Gadjah Mada

Jl. Fauna No.2, Karangmalang, Yogyakarta

Phone: 0274-560862

Fax: 0274-560861

Email: jsv_fkh@ugm.ac.id

Jurnal Sain Veteriner is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 

free
web stats View My Stats