Potensi Ekstrak Atuna racemosa sebagai Anti - Methicillin Resistant Staphylococcus aureus (MRSA)

https://doi.org/10.22146/jsv.34700

Siti Isrina Oktavia Salasia(1*), Novra Arya Sandi(2), Fajar Budi Lestari(3), Verda Farida(4), Nurbani Aziz(5)

(1) Departemen Patologi Klinik, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Jl. Fauna 2, Karangmalang, Yogyakarta
(2) Departemen Patologi Klinik, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Jl. Fauna 2, Karangmalang, Yogyakarta
(3) Departemen Teknologi Hayati dan Veteriner, Program Studi Diploma Kesehatan Hewan, Jl. Yacaranda, Sekip unit II, Yogyakarta
(4) Fakultas Farmasi, Universitas Ahmad Dahlan, Yogyakarta
(5) Departemen Patologi Klinik, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Jl. Fauna 2, Karangmalang, Yogyakarta
(*) Corresponding Author

Abstract


Staphylococcus aureus is one of the major causative agents of mastitis in animals and a variety of human diseases such as septicemia, endocarditis, arthritis dan osteomyelitis. Infection of Methicillin-resistant S. aureus (MRSA) has been widely reported and these strains are usually resistant to multiple antibiotics. The purpose of this study was to evaluate the potential of Atuna racemosa, as an alternative herbal medicine against MRSA infection. The MRSA strains were isolated from human and confirmed based on their resistant to various antibiotics and analyzing of the mecA gene by polymerase chain reaction (PCR). Atuna racemosa originated from Ambon, Maluku, Indonesia, were extracted using 70% ethanol. The activities of the Atuna racemosa extract against MRSA were performed by diffusion disc agar and dilussion agar tests. The results showed that Atuna racemosa extract has the barrier effect of MRSA growth at a concentration of 5% in the diffusion test and at a concentration of 7% in the dilution test. Atuna racemosa could be used as an alternative new drugs with dose of 0.07 g/ml (7%) against MRSA which is multi-resistant to many antibiotics.

 


Keywords


Atuna racemosa; MRSA; multi-resistant; antibacterial; multi-resisten; antibakterial

Full Text:

PDF


References

Akineden, Ö., Annemüller, C., Hassan, A.A., Lämmler, C., Wolter, W., and Zschöck, M. (2001). Toxin genes and other characteristics of Staphylococcus aureus isolates from milk of cows with mastitis. Clin. Diagn. Lab. Immunol. 8: 959-964.

Bernal, P., Zloh, M., and Taylor, P.W. (2009). Disruptioun of D-alanyl esterification of Staphylococcus aureus cell wall teichoic acid by the β-lactam resistance modifier (-) epicatechin gallate. J. Antimicrob. Chemo. 63: 1156-1162.

Buenz, E. J., Bauer, B.A., Schnepple, D.J., Roedler, D.L.W., Vandell, A.G., and Howe, C.L. (2007). Randomized phase I study of Atuna racemosa: A potential new anti-MRSA natural product extract. J. Ethnopharmacol. 114: 371–376.

Caturla, N., Vera-Samper, E., Villalain, J., Mateo, C.R., and Micol, V. (2003). The relationships between the antioxidant and the antibacterial properties of galloylated catechins and the structure of phospholipid model membranes. Free Radical Biol. Med. 34 (6): 648-662.

Chen, C.J. and Huang, Y.C. (2014). New epidemiology of Staphylococcus aureus infection in Asia. Clin. Microbiol. Infect. 20 (7): 605-623.

Dinges, M. M., Orwin, P. M., Schlievert, P. M. (2000). Enterotoxin of Staphylococcus aureus. Clin. Microbiol. Rev. 13:16-34.

Gordon, R.J. and Lowy, F.D. 2008. Pathogenesis of methicillin-resistant Staphylococcus aureus Infection. Clin. Infect. Dis. (46):350-359.

Hayakawa, Y., Hashimoto, N., Imaizumi, K., Khaido, T. and Takeuchi, S. (2001). Genetic analysis of exfoliative A producing Staphylococcus aureus isolated from mastitis cows milk. Vet. Microbiol. 78: 39-48.

Ikigai, H., Nakae, T., Hara, Y., and Shimamura, T. (1993). Bactericidal cathechins damage the lipid bilayer. Biochimica et Biophysica Acta e Biomembranes. 1147: 132-136.

Jang, S.S., Biberstein E.L. and Hirsh D.C. (1978). A diagnostic manual of veterinary clinical bacteriology and micology. UNESCO/CIDA Regional Training Course in Veterinary Diagnostic Microbiology, Peradeniya, 67 – 69.

Khanna, T., Friendship, R., Dewey, C., and Weese, J.S. (2008). Methicillin resistant Staphylococcus aureus colonization in pigs and pig farmers. Vet. Microbiol.. 128: 298.

Laplana, L. M., Cepero, MPG., Ruiz, J., Zolezzi, P.C., Calvo, M.C.R., Erazo, M.C., and Gomez-Luiz, R. (2007). Molecular typing of Staphylococcus aureus clinical isolates by pulsed-field gel electrophoresis, staphylococcal cassette chromosome mec type determination and dissemination on antibiotic resistance genes. Int. J. Antimicrob. Agents. 30: 505 -509.

Le loir, Y., Baron, F and Gautier, M. (2003). Staphylococcus and food poisoning. Genet. Mol. Res. 2 (1): 63-76.

Lee, J.C., Xu, S.L., Albus, A., and Livolsi, P.J. (1994). Genetic analysis of type 5 capsular polysaccharide expression by Staphylococcus aureus. J. Bacteriol. 27, 4883-4889.

Marrack, P. and Kappler, J. (1990). The staphylococcal enterotoxin and their relatives. Science. 248: 705-711.

Mendel, F. (2007) Overview of antibacterial, antitoxin, antiviral and antifungal activities of tea flavonoids and teas. Mol. Nutr. Food Res. 16:225-229.

Na’was, T., Hawwari, A., Hendrix, E., Hebden, J., Edelman, R., Martin, M., Campbell, W., Naso, R., Schwalbe, R., and Fattom, A.I. (1998). Phenotypic and genotypic characterization of nosocomial Staphylococcus aureus isolates from trauma patients. J. Clin. Microbiol. 36 (2): 414-420.

Nakayama, M., Shigemune, N., Tsugukuni, T., Jun, H., Matsushita, T., Mekada, Y., Kurahachi, M., Miyamoto, T. (2012). Mechanism of the combined anti-bacterial effect of green tea extract and NaCl againts Staphylococcus aureus and Escherichia coli O157:H7. Food Control. 25: 225-232.

Noreen,Yl., Serrano, G., Perera, P,. and Bohlin, L. (1998). Flavan-3-ols isolated from some medicinal plants inhibiting COX-1 and COX-2 catalysed prostaglandin biosynthesis. Planta Med. 64: 520-524.

Omoe, K., Ishikawa, M., Shimoda, Y., Hu, D. –L., and Ueda, Shinagawa, K. (2002). Detection of seg, seh, and sei genes in isolates and determination of the enterotoxin productivities of S. aureus isolates harbouring seg, seh, and sei genes. J. Clin. Microbiol. 40: 857-862.

Prance, GT. (2004). The uses of Atuna racemosa Raf. (Chrysobalanaceae) in Samoa. Economic Botany. 58(3): 470 – 475.

Rahmatan, H,. Iswadi, Hafizha, M. (2014). Daya hambat ekstrak rimpang kencur (Kaempferia galanga L.) Terhadap Pertumbuhan Bakteri Escherichia coli. Prosiding Seminar Nasional Biologi: 40-46.

Salasia, S.I.O., Sandi, N.A., Widianingrum, D.C., Windria, S. and Farida, V. (2016). Detection of mecA gene of Staphylococcus aureus isolated from dairy cows and human in Yogyakarta, Indonesia. 8th International Seminar of Indonesian Society for Microbiology (ISISM). Jakarta, August 11th – 12th , 2016.

Salasia, S. I. O., Khusnan., Lämmler, C., Zshöck, M. (2004). Comparative studies on pheno- and genotypic properties of Staphylococcus aureus isolated from bovine subclinical mastitis in Central Java, Indonesia and Hesse, Germany. J. Vet. Re.s Sci. 5(2): 103- 109.

Suryawiria (1978). Mikroba Lingkungan. Edisi ke 2. ITB: Bandung.

Takeuchi, S. Maeda, T., Hashimoto, N., Imaizumi, K., Kaidoh, T. and Hayakawa, Y. (2001). Variation of the agr locus in Staphylococcus aureus isolates from cows with Mastitis. Vet. Microbiol. 79 (3) : 267-274

Todar, K. (2002) Staphylococcus. Bacteriology at UW-Bacteriology 330. Home Page. 1–7.

Toshkova, K. Annemūller, C. and Lämmler, C. 2001. The significance of nasal carriage of Staphylococcus aureus as risk factor for human skin infections. FEMS Microbiol. Lett. 202: 17-24.

Wishart, K., Goldsmith, C.E., Loughrey, A., McClurg, B. and Moore, J.E. (2008). Methicillin-resistant Staphylococcus aureus: A microbiological update. Biomed. Scientist, Jano 08: 39 – 41.

Woods, G.I., and Washington, J.A. (1995). Manual of clinical microbiology, 6th ed, ASM Pess, Washingtong, D. C. Pp. 327-341.



DOI: https://doi.org/10.22146/jsv.34700

Article Metrics

Abstract views : 536 | views : 302

Refbacks

  • There are currently no refbacks.




Copyright (c) 2018 Jurnal Sain Veteriner

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Sain Veteriner Indexed by

  CrossrefROADCOREDimensions  JournalTOCs   


Copyright of JSV (Jurnal Sain Veteriner) ISSN 0126-0421 (print), ISSN 2407-3733 (online).

Fakultas Kedokteran Hewan, Universitas Gadjah Mada

Jl. Fauna No.2, Karangmalang, Yogyakarta

Phone: 0274-560862

Fax: 0274-560861

Email: jsv_fkh@ugm.ac.id

Jurnal Sain Veteriner is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 

free
web stats View My Stats