Ekspresi IL – 1β pada Proses Kesembuhan Fraktur Os Femur Tikus Putih (Rattus norvegicus) Ovariektomi yang Diterapi dengan Ekstrak Cikal Tulang (Cissus quadrangularis)

https://doi.org/10.22146/jsv.29296

Ira Sari Yudaniayanti(1*), Bambang Sektiari Lukiswanto(2), M. Zainal Arifin(3)

(1) Fakultas Kedokteran Hewan, Universitas Airlangga
(2) Fakultas Kedokteran Hewan, Universitas Airlangga
(3) Fakultas Kedokteran Hewan, Universitas Airlangga
(*) Corresponding Author

Abstract


This study aims to determine the potential of the Cissus quadrangularis extract to decreased the expression of IL-1 β so that it can to inhibit bone resorption and osteoporosis fracture healing is not delayed union. Forty rats adapted for 1 week, and then divided into four groups with 10 replications. P0 (sham-operated + Osteotomy + CMC Na); P1 (ovariectomy + Osteotomy + CMC Na); P2 (ovariectomy + Osteotomy + Raloxifene 5.4 mg /kg); P3 (ovariectomy + Osteotomy + CQ 750 mg / kg). Osteoporosis induced is made by bilateral ovariectomy in rats, then to make sure that osteoporosis is already happened, 8 weeks post-ovariectomy performed radiology examinations on the femur, then the osteotomy action. IL1-β expression observations performed in at the 2nd week and 6th week after osteotomy through the immunohistochemistry examination. The results of immunohistochemistry examination in the 2nd week showed an increased expression of IL1-β in all groups of rat that did ovariectomy (P1, P2 and P3) were significantly different (p <0.05) with a rat group that didn’t ovariectomy, as well P2 dan P3 were significantly different with P1. The Results of immunohistochemistry examination in the 6th week showed the same
pattern with the 2nd week and there is increased IL1-β expression in all groups of rat compared with the results of the examination in the 2nd week. Based on these results it can be concluded that Cissus quadrangularis extract and
Raloxifen can decreased the expression of IL-1 β, and the results that don’t significantly different, so that these both materials can to inhibit bone resorption and osteoporosis fracture healing don’t get delayed union.


Keywords


Cissus quadrangularis Extract; IL – 1 β; osteoporosis; ovariectomy; healing fracture of femur

Full Text:

PDF


References

Ardo K, Sunariani J. 2003. The Role of Interleukin-

β, Interleukin-6, Tumour Necrosis Factor-α

and Interferon γ in Bone Remodelling Process

during Orthodontic Treatment. University of

Airlangga;316-20

Bell, NH. (2003). RANK ligand and the regulation of

skletal remodeling. J Clin Invest; (111):1120-22.

Cao Y, Mori S, Mashiba T, Westmore MS, Ma L,

Sato M. (2002). Raloxifene, estrogen, and

alendronate affect the processes of fracture

repair differently in ovariectomized rats. J

Bone Miner Res 2002;17-12:2237–46.

Claes, L., Recknagel, S., Ignatius, A. (2012). Fracture

healing under healthy and inflammatoryconditions. Nat Rev Rheumatol ;8:133–43.

Deka, D.K., Lahon, L.C., Saikia, J., Mukit, A.

(1994). Effect of Cissus quadrangularis in

accelerating healing process of experimentally

fractured radius - ulna of dog: A preliminary

study. Ind J Pharmacol 26:44-8.

Dimitriou R, Tsiridis E, and Giannoudis P V. 2005.

Current concepts of molecular aspects of bone

healing, Injury (vol. 36), No. 12, pp. 1392-404.

URL:http://www.ncbi.nlm.nih.gov/pubmed/

Flecknell, P. (2009). Laboratory Animal Anaesthesia.

Academic Press is an imprint of Elsevier Edition

rd. Gerstenfeld LC, Edgar CM, Kakar S, et al, 2007.

Osteogenic Growth Factors and Cytokines and

Their Role in Bone Repair. In (Bronner F, Farach-

Carson MC, Mikos AG, eds). Engineering of

Functional Skeletal Tissues. Springer-Verlag.

London. 17-45.

Ghozali, I. (2006). Statitik Non-Parametrik Teori dan

Aplikasinya dengan Program SPSS. Badan

Penerbit Universitas Diponegoro. Semarang.

Jones, D.H., Kong, Y.Y., Penninger, J.M. (2002).

Role of RANKL and RANK in bone loss and

arthritis. Ann Rheum Dis;2:1132-9.

Kalu, D.N. (1991) The ovariectomized rat model of

postmenopausal bone loss. Bone Miner.;15(3):

-91.

Kanakamani, J., Tandon, N. (2008). Newer therapies in

osteoporosis. Indian Journal of Rheumatology.

Review Article.Vol.3, No 4; pp. 148–157

Kaveh, K., Ibrahim, R., Abu Bakar, M.Z., and

Ibrahim, T.A. (2010). Osteoporosis Induction

in Animal Model. Am. Jour. of Animal and

Veterinary Sciences 5 (2): 139-145

Ke-Rong Dai and Yong-Qiang Hao. 2005. Quality

of Healing Compared Between Osteoporotic

Fracture and Normal Traumatic Fracture.

Review. Department of Orthopaedics, Ninth

People’s Hospital, Shanghai Jiao Tong

University, school of Medicine, 639 Zhizaoju

Road, Shanghai, China

Killic, T.O. and Sagiroglu C. (2013). Anabolic Agents

as New Treatment Strategy in Osteoporosis.

Intech Open Science. hlm. 241-257.

Kyllönen, L., D’Este, M., Alini, M., Eglin, D.

(2015). Local drug delivery for enhancing

fracture healing in osteoporotic bone. Acta

Biomaterialia 11 412–434

Malutan, A.M., Dan, M., Nicolae, C., Carmen, M.

(2014). Proinflammatory and anti-inflammatory

cytokine changes related to menopause. Prz

Menopauzalny; 13(3): 162-168

Mountziaris, P.M, and Mikos, A.G. (2008).

Modulation of the inflammatory response for

enhanced bone tissue regeneration. Tissue

Engineering Part B: Reviews; 14, 179-86

Muthusami, S., Ramachandran, I., Krishnamoorthy, S.,

Govindan, R., Narasimhan, S. (2011). Cissus

quadrangularis augments IGF system components

in human osteoblast like SaOS-2 cells. Growth

Hormone & IGF Research 21: 343–348

Nazrun, A.S., Norazlina, Norliza M., and Nirwana

S.I. (2012). The Anti-Inflammatory Role of

Vitamin E in Prevention of Osteoporosis.

Advances in Pharmacological Sciences.

Volume 2012,Article ID 142702, 7 pages

Nikolaou, V.S., Efstathopoulos, N., Kontakis, G.,

Kanakaris, N.K., Giannoudis, P.V. (2009). The

influence of osteoporosis in femoral fracture

healing time. Injury ;40:663–8.

Parisuthiman, D., Singhatanadgit, W., Dechatiwongse, T.,

Koontongkaew, S. (2009). Cissus quadrangularis

extract enhances biomineralization through

up-regulation of MAPK-dependent alkaline

phosphatase activity in osteoblasts. In Vitro Cell.

Dev.Biol.-Animal;45: 194-200.

Potu, B.K., Bhat, K.M.R, Rao, M.S., Nampurath,

G.K., Chamallamudi, M.R., Nayak, S.R.,

Muttigi, M.S. (2009). Petroleum ether extract

of cissus quadrangularis (linn.) enhances

bone marrow mesenchymal stem cell

proliferation and facilitates osteoblastogenesis.

Clinics.;64(10):993-998.

Rosen dan Clifford.( 2005). Postmenopausal

Osteoporosis. N Engl J. Med.;353: 595-603

Sabri, M., Nurhidayat, Sigit, K., Priosoeryanto, B.P.,

Manalu, W. (2009). Analysis of phytochemical

and mineral content of Sipatah-patah Plant

(Cissus quandrangularis) from Aceh as

osteoporosis premedication. J Rona Lingkungan

: 109-117.

Schmidt-Bleek K, Schell H, Kolar P, Pfaff M, Perka

C, Buttgereit F, Duda G and Lienau J. 2009.

Cellular composition of the initial fracture

hematoma compared to a muscle hematoma: A

study in sheep, J Orthop Res (vol. 27), No. 9, pp.

-51. URL: http://www.ncbi.nlm.nih.gov/

entrez/query.fcgi?cmd=Retrieve&db=PubMed

&dopt=Citation&list_uids=19382195 Sfeir C,

Ho L, Doll BA, et al (2005). Fracture Repair.

In (Lieberman JR, Friedlander GE, eds). Bone

Regeneration and Repair, Biology and Clinical

Application Humana Press, Totowa. New Jersey.

-44.

Shirwaikar, A., Khan, S., Malini, S. (2003).

Antiosteoporotic effect of ethanol extract of

Cissus quadrangularis Linn. on ovariectomized

rat. J Ethnopharmacol 89: 245-250

Waters, K.M., Rickard, D.J., Gebhart, J.B. (1999).

Potential roles of estrogen reseptor-α and -β in the

regulation of human oteoblast functions and gene

expression. The menopause at the millenium. The

Proceding of the 9th International Menopause

Society World Congress on Menopause. October

-21; Yokohama, Japan.

Xu, S.W., Yu, R., Zhao, G.F., Wang, J.W. (2003). Early

period of fracture healing in ovariectomized

rats. Chin J Traumatol. 6:160-6.

Yamaguchi, M. (2002). Isoflavone and bone

metabolism: its cellular mechanism prevention

role in bone loss. J. Health Sci. 48(3):209-220.

Zaid, S.S.M., Sulaiman, S.A., Othman, N.H.,

Soelaiman, I.-N., Shuid, A.N., Mohamad, N.

and Muhamad, N. (2012). Protective Effects

of Tualang Honey on Bone Structure in

Experimental Postmenopausal Rats. J. Clinics,

vol. 67, no. 7, pp. 779-784.



DOI: https://doi.org/10.22146/jsv.29296

Article Metrics

Abstract views : 263 | views : 401

Refbacks

  • There are currently no refbacks.




Copyright (c) 2017 Jurnal Sain Veteriner

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Sain Veteriner Indexed by:


Copyright of JSV (Jurnal Sain Veteriner) ISSN 0126-0421 (print), ISSN 2407-3733 (online).

Fakultas Kedokteran Hewan, Universitas Gadjah Mada

Jl. Fauna No.2, Karangmalang, Yogyakarta

Phone: 0274-560862

Fax: 0274-560861

Email: jsv_fkh@ugm.ac.id

Jurnal Sain Veteriner is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 

free
web stats View My Stats