Kerusakan Usus pada Mencit (Mus musculus) yang Diinokulasi Larva 3 (L3) Anisakis spp.

https://doi.org/10.22146/jsv.29292

Febrina Dian Permatasari(1*), Poedji Hastutiek(2), Lucia Tri Suwanti(3)

(1) Fakultas Kedokteran Hewan, Universitas Airlangga
(2) Fakultas Kedokteran Hewan, Universitas Airlangga
(3) Fakultas Kedokteran Hewan, Universitas Airlangga
(*) Corresponding Author

Abstract


This study aims to be show damage in the intestine of mice caused by inoculation of the larvae of Anisakis spp. alive and who has died because of a warming 750C for 7 minutes. Eighteen male mice (Mus musculus) were divided into three groups: control group, a group of mice were inoculated the larvae of Anisakis spp. alive and
groups of mice were inoculated the larvae of Anisakis spp. who have died. 48 hours post inoculation, the mice’s intestines necropsy performed later performed HE staining to identify and scoring intestinal histopathology. The results showed inoculation of the larvae of Anisakis spp. either alive or dead induce histological changes in the intestine in the form of infiltration of inflammatory cells, epithelial changes and structural changes in the intestinal mucosa


Keywords


Third stage larvae Anisakis spp., Intestinal damage; histopathology; Mus musculus

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References

Asami K and Inoshita Y (1967) Experimental

anisakiasis in guinea pigs: Factors influencing

infection of larvae in the host. Japanese J of

Parasitol 16: 415–422.

Audicana, T.M and Kennedy, M.W. 2008. Anisakis

simplex: from obscure infectious worm

toinducer of immune hypersensitivity. Clin

Microbiol Rev. 21(2):360-379.

Buzzell, G.R and Sommerville, R.I (1985) The structure

of the oesophagus in the third-stage infective

larva of Anisakis sp. (Nematoda: Anisakidae).

Trans Am Microsc Soc. 104: 86-94.

Caballero, M.L and Moneo, I (2004)Several allergens

from Anisakis simplex are highly resistant to heat

and pepsin treatments. Parasitol Res. 15:1-7.

Caballero, M.L. Moneo, I. Gomez-Aguado, F.

Corcuera, M.T. Casado, I and Rodriguez- Perez,

R (2008) Isolation of Ani s 5, an excretorysecretory

and highly heat-resistant allergen

useful for the diagnosis of Anisakis larvae

sensitization. Parasitol Res. 103(5):1231-1233.

Daschner, A. Cuéllar, C and Rodero, M.,2012. The

Anisakis allergy debate: does an evolutionar62

Febriana Dian Permatasari et al.

yapproach help? Trends Parasitol.28:9–15.

http://dx.doi.org/10.1016/j.pt.2011.10.001.

Diakses pada tanggal 7 Agustus 2016.

Erben, U. Loddenkemper, C. Doerfel, K.

Spieckermann, S. Haller, D. Heimesaat, M.M.

Zeitz, M. Siegmund, B and Kühl, A.A (2014)

A guide to histomorphological evaluation of

intestinal inflammation in mouse models. Int J

Clin Exp Pathol. 7(8):4557-4576.

Fukuda T, Tongu Y and Ishii A (1990) Ultrastructural

changes in the oesophagus, intestine,

and excretory organ of larval Anisakis

(Ascaridoidea: Nematoda) after incubation in

arti®cial gastric juice. Jpn J Parasitol 39: 1-6.

Gomez-Aguado, F. Picazo, A. Caballero, M.L.

Moneo, I. Asturias, J.A and Corcuera, M.T

(2003) Ultrastructural localization of Ani s 1, a

major allergen from the fish parasite Anisakis

simplex. Parasitol Res. 89:379–380.

Hariyadi, A.S (2006) Pemetaan infestasi cacing parasitik

dan risiko zoonosis pada ikan laut di perairan

Indonesia Bagian Selatan. Bogor. IPB.

Iglesias, L. Valero, A. Banitez, R. and Adroher, F.J

(2001) In vitro cultivation of anisakissimplex:

pepsin increases survival and moulting from

fourth larval to adult stage. Parasitol. 123:285-

Lindqvist, A. Ikezawa, Z. Tanaka, A and Yman,

L (1993) Seafood specific IgE in atopic

dermatitis. Ann Allergy. 70:58.



DOI: https://doi.org/10.22146/jsv.29292

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