Efektivitas Larutan Desinfektan Dalam Menginaktivasi Virus Avian Influenza pada Bulu Unggas


Bagus Nanang Luwito(1*), I Wayan Teguh Wibawan(2), Retno Damayanti Soejoedono(3)

(1) Balai Karantina Pertanian Kelas I Balikpapan
(2) Fakultas Kedokteran Hewan Institut Pertanian Bogor
(3) Fakultas Kedokteran Hewan Institut Pertanian Bogor
(*) Corresponding Author


Avian Influenza (AI) virus  is pathogenic agent that can spread from one area to another area through the transportation of infected animals or their products such as feathers. This research was aimed to inactivated AI virus with 775 ppm sodium hypochlorite and 0.1% chloroxylenol to examine the difference of treatment by time (day) for inactivation AI virus on the feathers. AI virus isolate A/chicken/sidrap/ 07160336‐2/2016 used in this research was obtained from Balai Besar Veteriner Maros. The treatment of disinfectants were performed on the first day (the day of disinfectan solutions were prepared), the third day and the seventh day by soaked the feathers in disinfectant solution for 10 minutes. The effectiveness of disinfectans were evaluated by inactivation index. The result show that the average of inactivation index of 775 ppm of sodium hypochlorit was 4.17 for the first day, 5.17 for the third day, and 4.20 for the seventh day, while the average of inactivation index of 0.1% chloroxylenol was 5.50 for the first day, 6.43 for the third day, and 5.77 for the seventh day. Our result indicated that the sodium hypochlorit and chloroxylenol were effective for inactivation of AI virus. The 0.1% of chloroxylenol was more effective for inactivation AI virus than 775 ppm of sodium hypochlorit, whilst the most effective duration for the treatment is the three days.


Avian influenza virus; Chloroxylenol; Inactivation index; Sodium hypochlorit

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Alphin RL, Johnson KJ, Ladman BS, Benson ER. 2009. Inactivation of avian influenza virus using four common chemicals and one detergents. Poultry Sci. 88: 1181‐1185.

Birnbaum NG, O’brien B. 2008. Methods for inactivation of avian influenza virus in the environment. Di dalam: Swayne DE, editor. Avian Influenza. Blackwell.Iowa. USA: 391‐405.

Gamal AM, Samaha HA, Elagrab HM, Ismael IE, Nasr SAE, Moubarak ST, Hussein HA, Zahran ZM. 2014. Inactivation of avian influenza virus using commercial chemical disinfectans in small scale poultry production. Alexandria J Vet Sci. 41: 102‐108

Lombardi ME, Ladman BS, Alphin RL, Benson ER. 2008. Inactivation of avian influenza virus using common detergents and chemicals. Avian Dis. 52: 118‐123.

[OIE] Office International des Epizooties. 2015. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. Chapter 2.3.4. Avian Influenza. http://www.oie.int /international-standard-setting/terrestrial-manual/access-online/. diakses pada tanggal 28 April 2016

Pratiwi ST. 2008. Mikrobiologi Farmasi. Erlangga.Jakarta. Indonesia: 135‐147.

Reed LJ, Muench H. 1938. A simple method of estimating fifty percent end points. Am. J. Hyg. 27: 493‐497.

Siswandono, Soekardjo B. 2000. Kimia Medisinal. Volume ke‐2. Airlangga University Press. Surabaya. Indonesia: 7‐82.

Slomka MJ, Coward VJ, Banks J, Londt BZ, Brown IH, Voermans J, Koch G, Handberg KJ, Jorgensen PH, Cherbonel-Pansart M et al. 2007. Identification of Sensitive and Specific Avian Influenza Polymerase Chain Reaction Methods Through Blind Ring Trials Organized in the European Union. Avian Dis. 51: 227‐234.

Suarez DL, Spackman E, Senne DA, Bulaga L, Welsch AC, Froberg K. 2003. The effect of various disinfectants on the detection of avian influenza virus by real time RT-PCR. Avian Dis. 47: 1091‐1095.

Tong S, Li Y, Rivailler P, Conrardy C, Castillo DAA, Chen LM, Recuenco S, Ellison JA, Davis CT et al. 2012. A distinct lineage of influenza A virus from bats. Proc. Natl. Acad. Sci. U. S. A. 109: 4269‐4274.

Van den Berg T. 2009. The role of the legal and illegal trade of live birds and avian products in the spread of avian influenza. Rev. Sci. Tech. 28: 93‐111.

Wanaratana S, Tantilertcharoen R, Sasipreeyajan J, Pakpinyo S. 2010. The inactivation of avian influenza virus subtype H5N1 isolated from chickens in Thailand by chemical and physical treatments. J.Vetmic.140: 43-48.

[WHO] World Health Organization. 2007. Recommendations and laboratory procedures for detection of avianinfluenza A(H5N1) virus in specimens from suspected human Cases. http://www.who.int/influenza/resources/documents/Rec AIlabtes tsAug07.pdf. diakses pada tanggal 6 Juni 2017.

Yamamoto Y, Nakamura K, Yamada M, Mase M. 2010. Persistence of avian influenza virus (H5N1) in feathers detached from bodies of infected domestic ducks. Appl Environ Microbiol. 76: 5496‐5499.

DOI: https://doi.org/10.22146/jsv.26934

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