Collection and Preservation of the Sperm Garangan ((Herpestes javanicus)

https://doi.org/10.22146/jsv.2619

Alvien Nuraini(1*), Ni Luh Putu Rischa Phadmacanty(2), Yulianto Yulianto(3)

(1) Jurusan Biologi Universitas Negeri, Jember
(2) Pusat Penelitian Biologi LIPI, Cibinong, Bogor
(3) Pusat Penelitian Biologi LIPI, Cibinong, Bogor
(*) Corresponding Author

Abstract


Cryopreservation technique can be used to preserve animal cell, plant, or other genetic materials  including  frozen semen. Herpestes javanicus is one of wild animals with a large number but no data about the sperm quality and technique how to preserve it. This research is proposed to know sperm quality of H. javanicus and the technique to preserve it. In this research, the three different methods were used to collect sperms. Those methods were  electroejaculator, flushing  and maceration/cutting, respectively. The sperm was then analyzed for quality, such as:  volume, pH, viscosity, colour, smell, motility, concentrasion, viability, morphology and morphometry. Then the sperms were  preserved in tris citrate glucose egg yolk extender by different concentrations. The result showed that the best method for collecting sperms was  by maceration/cuting method, and the best extender for sperms was low concentration of tris cytrate glucose egg yolk.

 Keywords: cryopreservation, Herpentes javanicus, sperm quality, preserve method, extender

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DOI: https://doi.org/10.22146/jsv.2619

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