Karakterisasi Antibodi Poliklonal terhadap Aflatoksin M1

https://doi.org/10.22146/jsv.22807

Angriani Fusvita(1*), Romsyah Maryam(2), Eko Sugeng Pribadi(3)

(1) Departemen Ilmu Penyakit Hewan dan Kesehatan Masyarakat Veteriner, Institut Pertanian Bogor
(2) Balai Besar Veteriner Bogor
(3) Departemen Ilmu Penyakit Hewan dan Kesehatan Masyarakat Veteriner, Institut Pertanian Bogor
(*) Corresponding Author

Abstract


The aim of the research was characterized the polyclonal antibody at AFM for diagnostic reagens on track 1 and measure the levels of AFM in milk. The series of experiments are given a polyclonal antibody testing 1 dimetilaminobenzidin (DAB) by dot blot immunoassay (DBIA) so it will look brown color display on the positive control sample; purification and estimation antibody of AFM concentration with ammonium sulfate; 1 dialysis; fractionation using HiTrap Protein A HP column, measured using a spectrophotometer at a wavelength of 280 nm; and characterized of IgG using SDS-PAGE. DBIA test results showed a typical reaction between
antigen AFM1-BSA with AFM1-BSA antibody to rabbit serum in the form of brown dots after addition of DAB substrate. The results of spectrophotometric against rabbit serum fractionation showed the type of IgG heavy chain.


Keywords


Aflatoxin M; polyclonal antibody; IgG

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DOI: https://doi.org/10.22146/jsv.22807

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