Suplementasi Calcitriol Menurunkan Risiko Osteoporosis Tikus Ovariektomi

https://doi.org/10.22146/jsv.17887

Hartiningsih .(1*), Devita Anggraeni(2)

(1) Departemen Ilmu Bedah dan Radiologi, Fakultas Kedokteran Hewan, UGM
(2) Departemen Ilmu Bedah dan Radiologi, Fakultas Kedokteran Hewan, UGM
(*) Corresponding Author

Abstract


Suplemen calcitriol memicu pembentukan tulang, namun juga meningkatkan resorpsi tulang. Penelitian ini dilakukan untuk mengkaji efektivitas calcitriol dalam menurunkan risiko osteoporosis tikus ovariektomi. Lima belas tikus Wistar betina umur delapan minggu, dibagi tiga kelompok (kontrol normal KN, kontrol ovariektomi KOV, dan ovariektomi+ calcitriol OVD ) dan diberi perlakuan selama delapan minggu. Pada akhir perlakuan, 100 dilakukan pengambilan darah melalui pleksus retroorbitalis untuk pemeriksaan estradiol, selanjutnya semua tikus dietanasi menggunakan campuran ketamin10% dengan xylaxine 2%. Femur kiri diambil untuk pemeriksaan histopatologis. Hasil analisis menunjukkan tikus KOV mempunyai konsentrasi estradiol yang secara signifikan lebih rendah dibandingkan tikus KN, dan tikus OVD mempunyai konsentrasi estradiol tidak 100 berbeda signifikan dibanding tikus KOV. Gambaran histopatologis epifisis tulang femur distalis tikus OVD 100
terlihat mempunyai lebih sedikit jaringan adiposit dan lebih banyak spikulum trabekula dibanding tikus KOV. Dari hasil penelitian disimpulkan bahwa suplementasi calcitriol 100ng/hari selama delapan minggu pada tikus ovariektomi menurunkan risiko osteoporosis.

Keywords


tulang, Calcitriol, osteoporosis, ovariektomi

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DOI: https://doi.org/10.22146/jsv.17887

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