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Deteksi Keragaman Virus Tungro dari Beberapa Daerah Endemis di Indonesia dengan Teknik PCR-RFLP


 
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1. Title Title of document Deteksi Keragaman Virus Tungro dari Beberapa Daerah Endemis di Indonesia dengan Teknik PCR-RFLP
 
2. Creator Author's name, affiliation, country R. Heru Praptana; Sekolah Pascasarjana Universitas Gadjah Mada; Indonesia
 
2. Creator Author's name, affiliation, country Y. B. Sumardiyono; Fakultas Pertanian Universitas Gadjah Mada; Indonesia
 
2. Creator Author's name, affiliation, country Sedyo Hartono; Fakultas Pertanian Universitas Gadjah Mada; Indonesia
 
2. Creator Author's name, affiliation, country I Nyoman Widiarta; Pusat Penelitian dan Pengembangan Tanaman Pangan; Indonesia
 
2. Creator Author's name, affiliation, country Muhammad Muhsin; Balai Besar Biogen; Indonesia
 
3. Subject Discipline(s)
 
3. Subject Keyword(s) endemic areas and molecular variability in RTV; RTBV; RTSV; tungro; tungro disease
 
4. Description Abstract

Tungro is one of rice disease caused by two different viruses (rice tungro virus=RTV) i.e. Rice tungro bacilliform virus (RTBV) and Rice tungro spherical virus (RTSV) that are transmitted only by green leafhopper. Tungro had become a serious problem in several rice productions centre in Indonesia. Various components of management effort have been applied but they were inefficient in preventing the tungro disease development. Resistance variety is the most efficient component to tungro disease management. Complexity interactions of tungro disease components are mayor constraint in tungro disease management. Detection of molecular variability in rice tungro virus from several endemic areas in Indonesia were conducted by using PCR-RFLP technique. Existence of RTBV and RTSV in the infected plants collected from several endemic areas were successfully detected by PCR. The RFLP analysis with restriction enzymes BstYI and HindIII showed that there were significant difference among the RTSV originated from Java, Bali and Sulawesi.

 
5. Publisher Organizing agency, location Universitas Gadjah Mada
 
6. Contributor Sponsor(s)
 
7. Date (YYYY-MM-DD) 2009-07-28
 
8. Type Status & genre article
 
8. Type Type
 
9. Format File format PDF
 
10. Identifier Uniform Resource Identifier https://jurnal.ugm.ac.id/jpti/article/view/11763
 
10. Identifier Digital Object Identifier (DOI) https://doi.org/10.22146/jpti.11763
 
11. Source Title; vol., no. (year) Jurnal Perlindungan Tanaman Indonesia; Vol 15, No 1 (2009)
 
12. Language English=en en
 
14. Coverage Geo-spatial location, chronological period, research sample (gender, age, etc.)
 
15. Rights Copyright and permissions Copyright (c) 2009 Jurnal Perlindungan Tanaman Indonesia
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