* Corresponding Author
Exploration of Nematophagous Fungi from Coffee Rhizosphere Soil and their Potential as Biological Control Agents against Root-lesion and Root-knot Nematodes
https://doi.org/10.22146/jpti.102469 Siwi Indarti(1*), Rinda Fajrin Aldina(2), Donny Widianto(3), Irfan Dwidya Prijambada(4), Rina Maharani(5), Irianti Kurniasari(6), Dirk De Waele(7)
(1) Department Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Jln. Flora, Bulaksumur, Depok, Sleman, Yogyakarta 55281 Indonesia
(2) Department Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Jln. Flora, Bulaksumur, Depok, Sleman, Yogyakarta 55281 Indonesia
(3) Department of Microbiology, Faculty of Agriculture, Universitas Gadjah Mada, Jln. Flora, Bulaksumur, Depok, Sleman, Yogyakarta 55281 Indonesia
(4) Department of Microbiology, Faculty of Agriculture, Universitas Gadjah Mada, Jln. Flora, Bulaksumur, Depok, Sleman, Yogyakarta 55281 Indonesia
(5) Department Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Jln. Flora, Bulaksumur, Depok, Sleman, Yogyakarta 55281 Indonesia
(6) Agricultural Development Polytechnic of Malang, Jln. Dr. Cipto 144 A Bedali, Lawang, Malang, East Java, 65200 Indonesia
(7) Unit for Environmental Sciences and Management, North-West University, Private Bag X6001, Potchefstroom, 2520 South Africa; Laboratory of Tropical Crop Improvement, Department of Biosystems, Faculty of Bioscience Engineering, University of Leuven, Willem de Croylaan 42, Heverlee, 3001, Belgium
(*) Corresponding Author
Abstract
Root-lesion and root-knot nematodes are amongst the most important pathogens of coffee and can cause substantial yield losses and quality reductions. Environmental and health concerns concerning the use of chemical pesticides have increased the need for alternative management strategies against plant-parasitic nematodes. The aim of our study was to isolate and identify nematophagous fungi from nematode-infested coffee production areas and evaluate their potential as biocontrol agents. Our study was carried out in two stages: 1) fungi isolation and evaluation of their ability to affect eggs or vermiform developmental stages of root-lesion and root-knot nematodes; 2) identification of fungal isolates to species level. Eleven fungal isolates were able to affect either the nematode eggs or the vermiform developmental stages. The ability of these fungi to produce extracellular enzymes were also evaluated. This study highlights Indonesian nematode infected coffee rhizosphere soils as a rich source of nematophagous fungi, with eleven isolates showing promises to be used for integrated pest management strategies. Future work should assess field efficacy under local conditions while monitoring impacts on soil food webs and non-target organisms.
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DOI: https://doi.org/10.22146/jpti.102469
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