Fakultas Kedokteran Gigi Universitas Gadjah Mada Jl Denta no 1 Sekip Utara Yogyakarta, 55281 Telp 085643727254; Fax 0274-515307 http:jurnal.ugm.ac.id/jkg; e-mail: jkg@ugm.ac.id.
Fakultas Kedokteran Gigi, Universitas Gadjah Mada
Perbandingan Daya Antibakteri Disinfektan Instrumen Preparasi Saluran Akar Natrium Hipoklorit 5,25%, Glutaraldehid 2%, dan Disinfektan Berbahan Dasar Glutaraldehid terhadap Bacillus Subtilis
The aim of this study was to determine the differences between NaOCl concentration of 2.5% and 5.25% as irrigation material against push out bond strength of gutta percha root canal filler and epoxy resin along with resilon and methacrylate resin. The subjects of this study were 28 mandibular premolars. Root canals were prepared using rotary files up to #40/0.04. Subjects were divided into four groups of 7 teeth. IA group was irrigated using 2,5% NaOCl, followed by 17% EDTA and then obturated using gutta percha/ epoxy resin. IB group was irrigated using 2.5% NaOCl, followed by 17% EDTA and then filled with Resilon/methacrylate resin, IIA group was irrigated using 5.25% NaOCl, followed by 17% EDTA and then obturated by using gutta percha/epoxy resin, Group IIB was irrigated using 5.25% NaOCl, 17% EDTA and then obturated by using Resilon/methacrylate resin. The orifice filled with glass ionomer cement and stored in an incubator at a temperature of 370 C for 7 days. Specimens were then horizontally sectioned at apical third of 2 mm thickness and push out bond strength test were done with the Universal Testing machine. The data were analyzed with two way ANOVA and LSD test (á = 0.05%). adherence failure of root canal filling material observed with stereo microscope at 40x magnification. The mean push out bond strength of the group irrigated with 2.5% NaOCl and filled with gutta percha and epoxy resin was higher than the other groups. Two-way ANOVA test proved thatthere were significant difference of push out bond strength between groups and LSD test showed that a concentration of 2.5% NaOCl irrigation with gutta percha filler and epoxy resin sealer has the greatest mean significant difference from the other groups. It was concluded that push out bond strength with 2.5% NaOCl irrigation and gutta percha filling material and epoxy resin sealer was higher than the other groups.
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Pengaruh Jenis Surface Treatment dan Resin Komposit terhadap Kekuatan Tarik Reparasi Porselen
Composite resin is used to repair fractured porcelain because the esthetic reason and time saving. High tensile strength is usedto minimize the failure of porcelain reparation, so surface treatment that can increase tensile strength. The purpose of this study is to determine the influence of surface treatment (H3PO4 37%, HFA 9%, Sanblasting Al2O3) and the type of composites resin (Fowable and Packable) for porcelain reparation tensile strength. This study used 24 porcelain cylindrical samples with 5 mm in diameter and 3 mm in height. Samples were divided into 6 groups with 4 samples in each group. The first and second groups were etched with phosporic acid. The third and fourth groups were etched with hydrofluoric acid. The fifth and sixth groups were sanblasted with 50ìm Al2O3 for 5 seconds. The first, third and fifth groups were given a flowable composite resin and the second, fourth, and sixth group were applied a packable composite resin, and then the samples were tested with a tensile strength Universal Testing Machine. Data were analyzed by two way ANOVA and Least Significant Difference (LSD). The results show that there are significant difference between the surface treatment and the type of resin composite to tensile strength of porcelain reparation (p<0.05), but no significantly difference in the interaction between the surface treatment and the type of resin composite to tensile strength of porcelain reparation (p>0.05). The conclusion of this study issurface treatment using sandblast on porcelain reparation produce higher tensile strenght than using HFA 9% and H3PO4 37%, and packable composite resin produce higher tensile strenght than flowable composite resinon porcelain reparation.
Pengaruh Konsentrasi Natrium Hipoklorit sebagai Bahan Irigasi dan Jenis Bahan Bonding terhadap Kebocoran Mikro Resin Komposit Bulkfill Viskositas Rendah pada Dentin Kamar Pulpa
Instrumentation and sterilisation in root canal treatment procedure needs sodium hypochlorite as irrigation solution. Sodium hypochlorite is commonly used in the range of 2,5-5,25% concentration. Irrigation using sodium hypochlorite changes the structure of pulp chamber dentin. Final restoration can be established with bulkfill composite resin. Adhesion between composite resin and tooth structure is achieved by using either total-etch or self-etch adhesives and measured by microleakage rate. The aim of this research is to study influence of sodium hypochlorite concentration as irrigation material and adhesive systems to low-viscosity bulkfill composite resin microleakage on pulp chamber dentin. Twenty-four premolars were divided into three groups (control, NaOCl 2,5% and EDTA 17%, NaOCl 5,25% and EDTA 17%). Each group was divided into two subgroups (total-etch and bonding self-etch). Cavities on all specimens were restored with lowviscosity bulkfill composite resin. Specimens were centrifugated and soaked in methylene blue 2% then sectioned longitudinally. Methylene blue penetration was measured by stereomicroscope. Data was statistically analyzed with two-way ANOVA followed by LSD. Results showed that higher microleakage found in 5,25% concentration and total-etch group. Based on ANOVA test, sodium hypochlorite concentration and adhesive systems significantly influence microleakage. It can be concluded that 5,25% concentration sodium hypochlorite and total-etch adhesive resulted in higher microleakage of low-viscosity bulkfill composite resin on pulp chamber dentin.
Pengaruh Kombinasi Larutan Irigasi terhadap Kebocoran Apikal pada Obturasi Saluran Akar menggunakan Siler Resin Epoksi dan Mineral Trioxide Aggregate
The root canal treatment failure resulting from obturation stage wereunhermetic and cause apical leakage. The process of root canal treatment, the use of irrigation solution of sodium hypochlorite (NaOCl) or combined with ethylene diaminetetraacetic acid (EDTA), or plus chlorhexidine (CHX) as a root canal disinfection can cause changes in the condition of dentin and affects the attachment material sealer. The aim of this study is to determine the effect of NaOCl irrigation solution;the combination ofNaOCl and EDTA irrigation;the combination ofNaOCl, EDTA and CHX against apical leakage in the apical root canal obturation using material sealer of epoxy resin and MTA with a color penetration test method. The subjects are 24 premolars mandibular single root, decoronated and leaving a cut length of 14 mm from the apical teeth. All teeth are treated root canal preparation, with crown-down technique using ProTaper rotary files up to file F3. Subjects were divided into 3 treatment groups irrigation Group I: NaOCl irrigation, Group II: irrigation of NaOCl+EDTA, Group III: irigationof NaOCl+EDTA+CHX. Each group of irrigation divided into 2 treatment groupsobturation with Group A: obturation with epoxy resin sealer and Group B: MTA sealerobturation. The whole subject of research were incubated for 24 hours, and thereafter be stained with methylene blue solution. The whole subject of research is cut parallel to the longitudinal axis and observed under a stereo microscope with a magnification of 50x. The deepest penetration of the dye solution coming from the apical foramen are observed. Two way ANOVA and Post Hoc LSD are used to processing data in this study. Two way ANOVA showed that the combination of irrigation solution affect the leakage in the apical root canal obturation using epoxy resin sealer and MTA. LSD test results showed that there were differences between all groups combined irrigation solution. The conclusion of this study are: there is an effect of combinated irrigation solution in the apical leakage on obturation withsealer material of epoxy resin and mineral trioxide aggregate. There is an interaction of a combination of irrigation solution to the apical leakage using sealer material.
Ekspresi Syndecan-1, Matriks Metalloproteinase-2 dan IL-1α pada Ameloblastoma Multikistik (Penelitian Restrospektif Analitik di RSUP Dr. Sardjito Tahun 2010-2014)
Back ground : Characteristic of multycystic ameloblastoma is high recurency and high incidens. The aim of this study was to provide explanation of invasive ability, metastatic ability, growth progresivity, bone ostelysis, and recurrence of multicystic ameloblastoma in molecular level. Metodology : Paraffin block from Anatomical Pathology Installation of RSUP Dr. Sardjito 2010 – 2014, consisting of 14 follicular, 13 plexiform, 7 acantomathous, 2 granular, and 1 desmoplastic stained in HE and IHC Syndecan-1, MMP-2, and IL-1α were included in this study. The presence, localization, distribution pattern, and colour intensity of immunoreactive cells were observed using light microscope. The expression of Syndecan-1, MMP-2, and IL-1α were analyzed using Kruskall Wallis test, comparison using Mann-Whitney test, and correlation using Spearman rho test with significance P<0,05. Result : shows there are significant difference in immunoscoring expression of Syndecan-1 in epithel (P=0,031), MMP-2 (P=0,04), and IL-1α (P=0,049) in many types of Multicystic ameloblastoma. The results show corellation between MMP-2 and Syndecan-1 stroma expression (P=0,002) in acanthomatous type, IL-1α and Syndecan-1 epithel expression (P=0,00) in foliculair and acanthomatous type, also IL-1α and Syndecan-1stroma (P=0,00) in foliculair and plexiform type. Its show that Syndecan-1, MMP-2, and IL-1α synergicaly interracted to form an invasive and aggresive ameloblastoma. Conclution : Foliculair type, followed by acanthomatous type, shows more progressive in growth, invasive ability, metastatic ability, and recurrence compared with plexiform type.
Pengaruh Jenis Surface Treatment dan Semen Resin terhadap Kekuatan Geser Perlekatan Permukaan Zirconia dengan Semen Gigi Tiruan Cekat
Zirconia is used as indirect restorative dental material for fixed denture attached in tooth structure using resin cement. Zirconiasurface can attach well at resin cement if the surface treatment is done. The aim of this study is to evaluate the effect of the types of surface treatment and resin cement on shear bond strength of zirconia treatment with cement of fixed denture. It was used 24 samples of cylinder-shaped zirconia of 5 mm in diameter and 3 mm in height were divided into 3 groups of surface treatment: sandblast, MDP, and combination of sandblast and MDP. Each group was given self-adhesive and adhesive resin cements and was tested for shear strength by using a Universal Testing Machine. The data were analyzed by the two-way Anova and the Least Significant Difference (LSD) test. Results of the study show that there is significant difference between the types of surface treatmentand the types of resin cement in the shear strength of zirconia surface (p<0.05). There is no significant difference between interaction of the surface treatmentdan the types of resin cement in the shear strength of zirconia surface (p>0.05). The conclusion of this study are 1) the surface treatment by a combination of sandblastand MDP can increase the shear strength of zirconia surface attachment, and 2) the shear strength of attachment for adhesive resin cements was higher than self-adhesive resin cements.
Perbedaan Daya Antibakteri Siler Saluran Akar Berbahan Dasar Seng Oksid Eugenol, Resin Epoksi dan Mineral Trioxide Aggregate terhadap Enterococcus faecalls
Obturation is the last step in root canal treatment to prevent recolonization of bacteria remaining in the root canal and prevent the invasion of bacteria from the outside of the root canal, by closing the entire access that connects between periodontal and root canal. Selection of root canal sealer can support to the successfull of root canal treatment. Enterococcus faecalis is a bacteria that causing the failure of root canal treatment, because these bacteria can survive in the root canal that has been obturated. This study aims to determine the antibacterial activity of root canal sealer against E. faecalis. This study investigated the antibacterial activity of root canal sealer by using diffusion technique. E. faecalis with a density of 9 x 108 CFU/ml were inoculated in Mueller Hinton agar. Three wells made on agar medium with a diameter of 6 mm and a depth of 4mm at the same length in a petri dish measuring 10 mm x 100 mm. Each well at the bottom of the dish was marked by a marker to indicate a type of sealer inserted into the wells. Code A was a group of zinc oxide eugenol-based sealer, code B was a group of epoxy resin-based sealer and code C was a group of mineral trioxide aggregate-based sealer. Approximately 0.1 ml of each sealer was taken, included into the well by using plastic instrument and split injection of 1 ml. The plates were re-incubated at 37°C for 24 hours, then zones of inhibition were measured. The data obtained were subsequently recorded and analyzed by using the one-way ANOVA and the Least Significant Difference (LSD) test. ANOVA results indicate there are differences antibacterial activity by sealer based of zinc oxide eugenol, epoxy resin and mineral trioxide aggregate against E. faecalis (p <0.05). In the LSD test (p <0.05), the mean difference between the groups shows that sealer based of zinc oxide eugenol has an antibacterial activity better than epoxy resin and mineral trioxide aggregate against E. faecalis.
Pengaruh Suhu dan Penambahan Surfaktan pada Daya Antibakteri Sodium Hipoklorit terhadap Enterococcus faecalis
Antibacterial activity of NaOCl can improved by heated or by added surfactant, but heating is still debated as damaging its chemical structure. Enterococcus faecalis is bacteria which frequently found in root canal treatment failure. The aims of this study was to evaluate the influence of temperature and surfactant to antibacterial activity of NaOCl against E. faecalis.The study divided to four groups: NaOCl 5.25% at 280C and 450C, NaOCl 5.25% with added surfactants at 280C, and 450C. Nine petri dish containing MHA, each contain four wells that represent four research groups. The entire petri dish inserted into the anaerobic jar, incubated for 24 hours at 370C, and measured the inhibitory zone . Two way Anova show the influence of temperature and surfactant to the antibacterial activity of NaOCl against E. faecalis. Antibacterial activity of NaOCl 5.25% at 450C with added surfactant was greater than other groups. Antibacterial activity of NaOCl 5.25% with added surfactant is greater than 5.25% NaOCl without added surfactant at the same temperature. Antibacterial activity of NaOCl 5.25% at 450C was greater than NaOCl 5.25% at 280C, both in the group without added surfactant and with added surfactant.
Pengaruh Kandungan Surfactant Non-Ionik dalam NaOCl pada Irigasi dengan Teknik Manual, Sonik dan Endovac terhadap Kebersihan Sepertiga Apikal Permukaan Saluran Akar
Cleaning the root canal is done through root canal preparation using a mechanical instrument with irrigation using irrigation solution. Irrigation is an essential part of the root canal treatment, especially because of the difficulty of the mechanical preparation to reach the entire root canal walls. Irrigation solution most widely used is NaOCl. The addition of surface active agent (surfactant) in NaOCl solution can improve the effectiveness of irrigation. Irrigation techniques used also determine the effectiveness of the irrigation solution. The purpose of this study was to determine the effect of the combination of the addition of non-ionic surfactant in NaOCl with manual, sonic and EndoVac irrigation techniques on apical third of the root canal surface cleanliness. This study used 30 mandibular premolars, which were divided into 3 groups: I irrigated using manual techniques, II irrigated using sonic techniques, III irrigated using EndoVac. Each group was divided into two small groups, namely group IA, IIA, and IIIA irrigated with NaOCl, whereas the IB group, IIB and IIIB irrigated with NaOCl containing non-ionic surfactant. In the last irrigation after root canal preparation was completed, each of the teeth was irrigated with irrigation techniques and solutions in accordance with the group later split into two with the buccal-lingual direction. Apical third of the root canal cleanliness of debris and smear layer was observed by Scanning Electron Microscope. Data were analyzed using Kruskal-Wallis test followed by Mann-Whitney test. The results showed there was influence between the content of non-ionic surfactant in NaOCl and irrigation techniques to apical third of the root canal surface cleanliness of debris and smear layer. The content of non-ionic surfactant in NaOCl improved irrigation effectiveness in cleaning the apical third of the root canal surface from debris and smear layer.The sonic and EndoVac irrigation techniques were more effective than manual irrigation techniques, but use of NaOCl containing non-ionic surfactant would increase the effectiveness of the manual irrigation techniques as effective as sonic and EndoVac irrigation techniques.
Perbedaan Kebocoran Mikro Resin Komposit Bulk-Fill Viskositas Rendah dengan Teknik Penyinaran Ramped, Pulsed-Delay dan Konvensional pada Kavitas Kelas I
Low viscosity bulk fill composite resin is a restoration material with polymerization modulator on the monomer which serves to educe polymerisation shrinkage. Aim of this study was to determine the difference of low viscosity bulk fill composite resin microleakage using ramped, pulsed-delay and conventional photoactivation technique on cavity preparation class I. Twenty seven premolars were inserted by low viscosity bulk fill composite resin and lght-cured in accordance with one of the photoactivation techniques: ramped, pulsed-delay and conventional. Objects were soaked in 2% blue methylen solution for 24h, vertical divided and observed using stereo microscop at 50x magnification. The degree of microleakage was determined as the penetration depth of 2% blue methylen solution from coronal prior to apical in mm. Microleakage values weew analyzed using ANOVA (one-way analysis) and compared using post hoc test at 5% significance. According to the data, there was statistical difference between pulsed-delay and conventional (p<0,05), pulsed-delay and ramped (p<0,05), there was no statistical difference between ramped and conventional (p>0,05). Based on the result of this study, it can be concluded that the lowest value of microleakage was found for pulsed-delay from those of ramped and conventional.
Pengaruh Teknik Penyinaran ramped, Pulse-Delayed, dan Konvensional terhadap Kekerasan Mikro Resin Komposit Nanofil
Nanofill composite resin is dental composite that can be used for anterior or posterior restoration. Because it has good esthetic and good mechanical properties. The purpose of this study was to evaluate the influence of ramped, pulse-delayed, and conventional curing technique on microhardness of nanofill composite resin. Thirty nanofill composite resin mould were cured with ramped, pulse-delayed, and conventional technique. The objek were stored at incubator with 37° degree for 24 hours. Objects were indented with pyramid 136° angle for 15 seconds with 200 grams load. The value of microhardness were calculated by Vickers microhardness tester (Buehler, Jerman). Data were analyzed by one way ANOVA and compared using post hoc test at 5% significance. According the data, there was statistical difference between conventional and ramped (p<0,05), conventional and pulsedepayed (p<0,05), there was no statistical difference between ramped and pulse-delayed (p>0,05). Based on the result of this study, it can be concluded that the higher value of microhardness was found from covetional technique.
Penggunaan Suplemen Glukosamin-Kondroitin Sulfat peroral pada Pasien Osteoarthritis Pasca Scaling dan Root Planing (Kajian pada Gingival Index, Bleeding on Probing dan Pocket Depth)
Osteoarthritis and chronic periodontitis was an inflammatory disease. Osteoarthritis was occure in chronic, progressive and with pain. The chronic periodontitis was an inflammation of the tissues supporting the teeth resulting in a loss of adhesion of gingival and periodontal ligament damage. Supplements glucosamine-chondroitin sulfate on osteoarthritis could reduce pain and inhibit joint damage. The function of glucosamine-chondroitin sulfate on chronic periodontitis may modulate the host inflammatory response, regulate gene expression and decreased inflammatory mediators. This study aimed to determine the effect of supplement use glucosamine-chondroitin sulfate by oral in patients with osteoarthritis after scaling and root planing (SRP) with parameters of gingival index (GI), bleeding on probing (BOP) and pocket depth (PD). Subject study of 20 patients with osteoarthritis who experienced chronic periodontitis, divided into two groups, namely; SRP plus supplements glucosamine-chondroitin sulfate and SRP alone. Data retrieval GI, BOP and PD performed on days 0, 21st and 30th. Data were analyzed using t-test, Mann-Whitney and chi square test. Results of t-test and Mann-Whitney test showed a decrease in GI and PD in both groups, there were significant differences between the treatment group and the control group (p <0.05), whereas the chi square test against the decline of BOP, the result was not a significant difference between the treatment group and the control group. The conclusion of this study was the used of supplements glucosamine-chondroitin sulfate by oral in osteoarthritis patients who experience chronic periodontitis after SRP could decreas GI and PD, but does not apply to decreased BOP.
Pengaruh Aplikasi Topikal Propolis 10% terhadap Penyembuhan Luka Pasca Pencabutan Gigi Desidui Persistensi (Kajian pada Anak Usia 6-10 Tahun)
Tooth extraction leafe wound for several days. This healing process of wound through inflammatory, proliferative and maturation phases. Propolis contains of flavonoids and phenols (Caffeic acid phenethyl ester) which contain antioxidant, antibacterial and antiinflammatory agent that may accelerate the wound healing process. The aim of the research was to know the effect of apply topically of 10% propolis on the wound healing of persistent deciduous extraction. The quasi-experimental research was conducted on the 24 post-extraction wound of persistent deciduous where the 16 cases were treat with application topically of 10% propolis twice a day at day and night time for three days post extraction, and the rest as control with no application. Healing of the wounds were clinically observed by measuring the wound width with digital photo traced then analized with Google SkecthUp software; the color gradation of wound were compared with the normal tissue around; and the pain simptom based on the subject statement. The data were taken at the first, third, fifth, seventh and tenth days post tooth extraction. The data were statisticaly analized descriptively and Mann Whitney at 5% signifficant level. There was a signifficant difference (p <0.05) of the wound width reduction between the treatment group and the control at the third to tenth days post tooth extraction; while the color gradation treatmnet group relative faster than the control and with no pain symtom. It was concluded that apply topically of 10% propolis can accelerate the wound healing post extraction of persistent deciduous tooth.
Perbedaan Teknik Irigasi Saluran Akar menggunakan File Niti Rotary, Canal Brush dan Aktivasi Sonik terhadap Residu Kalsium Hidroksida pada Sepertiga Apikal Dinding Saluran Akar
Calcium hydroxide is intracanal medicament most widely used in multi-visit root canal treatment. Calcium hydroxide in root canal must be removed prior to obturation, because residual calcium hydroxide on root canal may lead to apical leakage. This can cause unhermetic root canal obturation. The aim of this study is to determine differences in root canal irrigation techniques using rotary NiTi file, canal brush and sonic activation of the residual calcium hydroxide in the apical third root canal wall. Twenty seven mandibular first premolars were used in this study. The crowns of the teeth were removed at the apical part of cemento enamel junction (CEJ ) 14 mm from apical. The canals were prepared with a crown down technique with ProTaper rotary files up to file F3. All subjects were applied with pasta calcium hydroxide + glycerin, then the specimens were randomly devided into 3 groups. Group 1 used rotary NiTi files, group 2 used canal brush and group 3 used sonic activation. Each specimen wassagittal devided, observed using stereo microscop at 30x magnification. The degree of calcium hydroxideresidue were calculated using image raster 3 software. Data were analyzed using one-way ANOVA and compared using post hoc test at 5% significance. According to the data, there was statistical difference between NiTi rotary file and canal brush (p<0,05), NiTi rotary file and sonic activation (p<0,05), there was no stastitical difference between canal brush and sonic activation (p>0,05). Based on the result of this study, it can be concluded that canal brush and sonic activation has the same ability which is better than NiTi rotary file in cleaning the residual calcium hydroxide in the apical third root canal wall.
Perbandingan Kebocoran Mikro antara Tumpatan Sementara Berbasis Resin, Kalsium Sulfat dan Seng Oksida Eugenol
Temprorary filling use in dentistry for short-term use over time during treatment, for example in root canal treatment. Temporary filling proper use of the success of root canal treatment. The purpose of this study was to determine differences in microleakage temporary filling based resin, calcium sulfate and zinc oxide eugenol. In this experiment, use 14 premolar. Teeth were divided into 3 groups based temporary filling. Group I is the tooth of filled with temporary filling based resin, the second group of filled teeth with temporary filling calsium sulfate-based and group III of filled teeth with temporary filling zink oxide eugenol-based. Leak test using methylene blue solution 2%. Observations microleakage done using a stereo microscope 30 and 100x magnifications. Leaks in this study sample revealed the presencce of methylene blue penetration among temporary filling material and cavity walls. The resulting data is ordinal data and analyzed using Kruskal Wallis test followed by Mann Whitney U test. Kruskal Wallis test result indicate that there are significant differences in microleakage between the three temporary filling while obtained p=0.000 (p<0,05). Mann Whitney U test result there differences in microleakage between the three temporary filling, filing-based resin while the lowest microleakage, then zinc oxide eugenol-based temporary micro highest leakage.
Perbedaan Kekerasan Mikro Giomer dan Kompomer setelah Prosedur In Office Bleaching menggunakan Bahan Karbamid Peroksida 45%
Teeth whitening techniques using carbamide peroxide has been approved in several major countries like USA, Canada, and Europe because it is safer, cheaper, and also effective for vital tooth whitening procedure than hydrogen peroxide. Giomer and Compomer are restorative material that combines the advantageous properties of composite resin with glass ionomer cement. The purpose of this study was to determine differences of giomer and compomer microhardness after the proceure in office bleaching using carbamid peroxide 45%. The study was conducted on 20 specimens giomer and 20 specimens compomer that have been applied using carbamid peroxide 45%. The specimens were stored in artificial saliva at a temperature 37°C for 7 days before microhardness was tested using the Vickers Hardness Tester (Buehler, Germany). Microhardness value were analyze by independence sample t test at confidence level 95% (a = 0,05). Independence sample t test results showed that there were significant differences in microhardness giomer and compomer after using bleaching agent carbamide peroxide 45% (p<0,05). The conclusion of this study is that there are differences in surface micro hardness giomer and compomer after treatment with carbamide peroxide 45%. Giomer microhardness is higher than compomer after treatment with bleaching agents carbamide peroxide 45%.
Pengaruh Hidrogen Peroksida 40% sebagai Bahan Bleaching terhadap Kekuatan Geser Pelekatan Braket Logam dengan Resin-Modified Glass Ionomer (Penelitian Eksperimental Laboratoris)
Some patients who are interested in orthodontic treatment might have already had their teeth bleached. There have been controversial reports regarding the interaction between bleaching agents and shear bond strength of brackets. The aim of this study was to determine the effect of 40% hydrogen peroxide as a bleaching agent and the time interval after bleaching to shear bond strength of the metal bracket bonded with a resin-modified glass ionomer, and bond failure site. Twenty-eight maxillary first premolar teeth were randomly divided into four groups (control, immediately, 2 weeks, 4 weeks after bleaching with 40% hydrogen peroxide). Six teeth from each group were test for shear bond strength and a tooth of each group were analysed by SEM. For all groups, the brackets were bonded with an RMGI. Samples were tested in shear mode on a UTM after bonding Edgewise brackets. The ARI scores were used to assess bond failure site. Statistical analysis was performed using one-way Anova and the Kruskal-Wallis. The results showed that the shear strength significantly different between groups, except between groups 2 weeks and 4 weeks after bleaching (p <0,05). The conclusion of this study was the application of hydrogen peroxide 40% decrease shear bond strength of the metal bracket. A delay of bonding for 2 weeks and 4 weeks after bleaching would increase shear bond strength of the metal bracket. Most bonding failure occurred at the enamel-adhesive interface.
Pengaruh Jenis Fiber dan Surface Treatment Ethyl Acetate terhadap Kekuatan Fleksural dan Impak pada Reparasi Plat Gigi Tiruan Resik Aklirik
Repaired acrylic denture should have adequate flexural and impact strength. Fiber and surface treatment ethyl acetate can be used to increase the strength of reparation. The aim of this study was to evaluate the effect of fiber material and ethyl acetate as surface treatment to flexural and impact strength of acrylic denture reparation. This study used 25 samples of acrylic resin plate wtih size 65x10x2.5 mm and 30x5x1 mm for cavity to measure flexural strength and other 25 samples of acrylic resin plate with size 55x10x10 mm and 26x5x4 mm for cavity to measure impact strength. Each measurement divided into five groups, with five samples for each group. This groups consist of control group (without fiber and ethyl acetate), group with ramie addition, group with ramie addition and ethyl acetate as surface treatment, group with polyethylene fiber addition, group with polyethylene fiber addition and ethyl acetate as surface treatment. Flexural strength measured with universal testing machine and impact strength measured with Charpy. The result analized with MANOVA followed by Least Significant Difference (LSD) with 95% significancy. The result showed that control group has the lowest flexural strength 71.11±3.41 MPa and impact strength 0.14±0.08 kJ/m2. Group with polyethylene fiber addition and ethyl acetate as surface treatment has the highest flexural strength 130.37±18.68 MPa and impact strength 1.15±0.09 kJ/m2. MANOVA showed there is significant difference of flexural and impact strength beacuse of fiber addition (p<0,05), no significant difference because of surface treatment ethyl acetate (p>0,05), and interaction between fiber and surface treatment doesn’t show any significant difference (p>0,05). The conclusion of this study is fiber material increased the flexural and impact strength of acrylic denture reparation but surface treatment didn’t increase the flexural and impact strength.
Perbedaan Konsentrasi dan Waktu Aplikasi EDTA sebagai Bahan Irigasi Saluran akar terhadap Pelekatan Push-Out Bahan Pengisi Saluran Akar
The effectiveness of EDTA as root canal irrigant might depend on concentration and application time. The purpose of this study was to determine the differences of EDTA in terms of concentrations (15% and 17%) and application times (1 minute and 3 minutes) on push-out bond strength of root canal filling using gutta percha and epoxy resin based sealer against root canal wall. This study used 28 mandibula premolars. The root canals were prepared using rotary files up to file #30/0,09. These specimen were divided randomly into 4 treatments group of 7 teeth each. Group IA was irrigated with 15% EDTA for 1 minute. Group IB was irrigated with 15% EDTA for 3 minutes. Group II A was irrigated with 17% EDTA for 1 minute and Group II B was irrigated with 17% EDTA for 3 minutes. Obturation was carried out using gutta percha and epoxy resin based sealer, and then stored in an incubator for 7 days. Specimens were then horizontally sectioned in apical third with 2 mm thickness. Root sections were tested with push-out technique, and observed under a stereo microscope (magnification of 40X) to determine the failure type. Data were analyzed with two-way ANOVA at 95% confidence level (α=0,05). A two-way ANOVA test revealed that there were no differences in push-out bond strength between concentrations (p>0.05), while a signifficant difference occurred between the application times (p<0.05). Moreover, no interaction occurred between concentration and application time on push-out bond strength. The failure type was observed predominantly as cohesive and the least for adhesive. Conclussion: Push out bond strength of root canal filling using EDTA irrigant solution with concentration of 15% was not different with 17%, whereas 3 minutes application time produced higher push-out bond strength than 1 minute.
Pengaruh Perawatan Ortodonsi Cekat Tahap Alignment pada Maloklusi Angle Klas I Berjejal Ringan terhadap Volume Vacum Oris dan Frekuensi Pengucapan Fonem /t/, /d/, /s/, /i/, /u/ dalam Morfem
Crowding affects aesthetics, performance, function of mastication, swallowing and speech. Treatment with fixed orthodontic appliances can correct crowding, changing the size of the dental arch and malocclusion and malrelation. Dental arch, alveolar arch and basal arch, high palate to the base of mouth is part of the cavum oris dimension. The purpose of this study was to determine the influence of fixed orthodontic treatment on alignment phase in Angle class I malocclusion with mild crowding case to cavum oris volume and speech frequency of phonemes /t/, /d/, /s/, /i/, /u/ in morpheme. Quasi-experimental research with one group pretest and post-test design, was performed in 10 children aged 12-13 years. Measurements of oral cavity and speech frequency of phonemes /t/, /d/, /s/, /i/, /u/ in morpheme were taken before and after the alignment. Data were analyzed by Paired t-test. The results showed there were significant difference cavum oris volume and speech frequency of phonemes /t/, /d/, /s/, /i/, /u/ between before and after alignment of fixed orthodontic treatment, on Angle Class I malocclusion with mild crowding case (p <0.05). Alignment phase increasing volume of cavum oris and decreasing speech frequency of phonemes /t/, /d/, /s/, /i/, /u/ in morphem.
Pengaruh Recombinant Human Erythropoietin terhadap Jumlah Osteoblas, Osteoklas dan kadar Hemoglobin pada Penyembuhan Fraktur Tulang Mandibula Studi Eksperimental pada Fraktur Mandibula Tikus Sprague-Dawley
Recombinant human erythropoietin (rHuEPO) is usually used as treatment choice for anemia due to end stage renal disease, but RHuEPO has reseptor in other tissues, so it has non erythroid effect. Its benefits in bone healing is still debated. This study aims to determine the effect of rHuEPO on the number of osteoblasts, osteoclasts and hemoglobin level in mandible fracture healing model.A controlled incomplete fracture mandibular symphysis was created surgically in twenty subjects of Sprague-Dawley rats. Ten subjects treatment group receive subcutaneous injection of 40 IU rHuEPO alpha for seven days, while ten subjects control got NaCl 0.9%. Hemoglobin of five subjects treatment group and five control were examined, then decapitated for HE histological evaluation on 7th and 14th day respectively. ANOVA and Paired T-test for hemoglobin data analysis based on study day, while the T-Independent test for number osteoblasts and osteoclast data based on study group.There were a significant difference on number of osteoblasts and osteoclasts 7th day compared to 14th day (p<0.05). Based on group, it revealed a significant difference in the number of osteoblasts (p=0.003), osteoclasts (p = 0.011) on 7th day, and osteoclasts number (p = 0.001) 14th day. Hemoglobin on 7th day had a significant difference compared to day 0 (p=0,001a and p=0,000b). Administration of rHuEPO enhance the number of osteoblasts and osteoclasts as well as hemoglobin on 7th day. On 14th day, only osteoclast number’s had significant difference and hemoglobin returned to baseline.
Regenerasi Nervus Mentalis akibat Cedera Penjepitan setelah Aplikasi Kombinasi Platelet Rich Plasma yang Diaktivasi Spons Kolagen dan Cytidine 5'-Diphosphocholine
Background. Regeneration of peripheral nerves after injury could be stimulated by adding growth factors from Platelet Rich Plasma (PRP) which activated with collagen sponge. Cytidine 5’-diphosphocholine (citicolin) is a neuroprotective agent which have antioxidant properties so that could overcome the PRP weakness that still contained neutrophils which could produce free radicals. The aim of this study is to determine the benefits of PRP were activated with collagen sponge combined with the provision of cytidine 5’-diphosphocholine towards the regeneration of mental nerve after crush injury. Methods. Thirty-six Wistar rats were divided into Sham operation and group with mental nerve injury (n=6). The injury group consisted of saline group, local application PRP which activated with collagen sponge, citicoline intraperitoneally 50 mg/100 gr weight, local application collagen sponge, and local application of PRP combined with citicoline intraperitoneally 50 mg/100 gr weight. Histological examination of nerve regeneration performed 4 weeks after mental nerve crush injury with histomorfometri parameters were the number of nerve fibers, nerve fiber diameter, axon diameter, and myelin thickness. The result data were analized by multivariate anova followed by post hoc test HSD. Result. Application combination of platelet rich plasma which activated with collagen sponge and cytidine 5-diphosphocolin (citicolin) significantly different than the salin group in increasing the number of nerve fibers and thick myelin (p<0,05) and same with Sham group (p> 0.05) in the measurement of the amount of fibers, nerve fiber diameter, the diameter of the axon and myelin thickness. The mean number of fibers, fiber diameter, the diameter of the axon and myelin thickness in the PRP group-citicolin higher than the PRP group, citicolin group, collagen sponge group and salin group. Conclusion. Application of a combination of platelet rich plasma activated collagen sponge and cytidine 5-diphosphocolin could improve nerve regeneration after injury.
PERBEDAAN PERUBAHAN WARNA PADA BAHAN RESTORASI GIOMER DAN KOMPOMER PASCA APLIKASI BAHAN BLEACHING BERBAHAN DASAR HIDROGEN PEROKSIDA 40% SEBAGAI BAHAN IN OFFICE BLEACHING
Color changes that occur in dental restorations after bleaching reduces the aesthetic aspects of the restoration that requires the replacement of the new restoration. Such procedures require more reduction of dental hard tissue that is not in line with the principle of minimum intervention. Therefore, it is necessary to look for restorative materials that are more resistant to bleaching materials. The purpose of this study was to determine the difference of color change in giomer and compomer after in office bleaching using hydrogen peroxide 40% based material. Sixteen specimens giomer and sixteen specimens compomer disc-shaped 20 mm diameter and 2 mm thick immersed in artificial saliva and kept in an incubator for 24 hours. All specimens were then measured using the initial color by chromameter with CIE L*a*b* system. All the specimens are then applied to materials made from hydrogen peroxide 40%. Specimens then soaked in artificial saliva for 24 hours. All specimens color were measured again and color differences between before and after application of bleaching materials have been calculated. The data were then analyzed the data using unpaired t test. The results showed a mean change of color (ΔE) at giomer higher than the compomer. Unpaired t test results showed significant difference between the two groups (α < 0.05). The conclusion from this study is the bleaching material made from hydrogen peroxide 40% cause color changes in giomer higher than compomer.
PERBANDINGAN DAYA ANTIBAKTERI DISINFEKTAN INSTRUMEN PREPARASI SALURAN AKAR NATRIUM HIPOKLORIT 5,25%, GLUTARALDEHID 2%, DAN DISINFEKTAN BERBAHAN DASAR GLUTARALDEHID TERHADAP Bacillus subtilis
Aseptic is a principle in dentistry that must be executed in daily practice by cross-infection control such as disinfection of endodontic instruments with high-level disinfectant (HLD). Some types of recognized high-level disinfectants are glutaraldehyde, glutaraldehyde-based disinfectant and sodium hypochlorite. The aim of this study is to compare the antibacterial potential of 5.25% sodium hypochlorite, 2% glutaraldehyde, and glutaraldehyde-based disinfectant on B. subtilis. Agar diffusion method was used in this study with 9 plate of Mueller-Hinton agar which was inoculated with B. subtilis culture McFarland scale 3. Three wells were made in each plate and each of the well was filled with a high-level disinfectant, which were 5.25% sodium hypochlorite, 2% glutaraldehyde, and glutaraldehyde-based disinfectant, then incubated and observed and measured its zone of inhibition. Data were analyzed with one-way ANOVA and post-hoc LSD test. One-way ANOVA result showed that 5,25% sodium hypochlorite, 2% glutaraldehyde, and glutaraldehyde-based disinfectant were influential as root canal preparation instruments disinfectants against B. subtilis. LSD test result showed that there were significant differences between 5.25% sodium hypochlorite and 2% glutaraldehyde, 5.25% sodium hypochlorite with glutaraldehydebased disinfectant; between glutaraldehyde 2% with glutaraldehyde-based disinfectant. The study concluded that 2% glutaraldehyde had the strongest antibacterial potential against B. subtilis, followed by glutaraldehyde-based disinfectant, and the weakest is 5.25% sodium hypochlorite.
PERBEDAAN DAYA ANTIBAKTERI MEDIKAMEN SALURAN AKAR BERBASIS SENG OKSIDA KOMBINASI KLINDAMISIN HIDROKLORIDA 5% DAN KALSIUM HIDROKSIDA TERHADAP BAKTERI Enterococcus faecalis (Penelitian Eksperimental Laboratoris)
Root canal medicaments have an important role to eliminate the remaining microorganisms in the root canal. Standard medicament that have long been used in dentistry is calcium hydroxide, but the material is resistant to Enterococcus faecalis. At this time, developed new medicament of zinc oxide base combined with clindamycin hydrochloride 5%. The purpose of this study was to determine differences in antibacterial effect of root canal medicament zinc oxide-based combination with clindamycin hydrochloride 5% and calcium hydroxide against Enterococcus faecalis. Nine pieces of petri dish containing media Mueller Hinton Agar (MHA) smeared with suspension of Enterococcus faecalis and made 3 holes with a diameter of 6 mm. Each hole were filled with 0.1 ml of zinc oxide combination with clindamycin hydrochloride 5%, calcium hydroxide combination with iodoform, and calcium hydroxide combination with glycerin using a tuberculin syringe, then incubated for 24 hours at 37°C. Formed diameter of inhibition zone was measured using a caliper. Tabulation of the data were analyzed using one way ANOVA. ANOVA results showed the influences of the three types of root canal medicaments against Enterococcus faecalis (p:0.000*). LSD test results showed a significant difference in the antibacterial activity between the treatment groups. From the results of this study it was concluded that zinc oxide combined with clindamycin hydrochloride 5% indicates the most effective antibacterial activity against Enterococcus faecalis.
PENGARUH METAL PRIMER DAN JENIS SEMEN RESIN TERHADAP KEKUATAN GESER PERLEKATAN LOGAM NIKEL-KROMIUM COPING GIGI TIRUAN CEKAT
Resin cement is often used for cementing restoration because oral fluid insolubility and increase marginal adaptation. High shear strength in resin cement is required to minimalize restoration cementation failure. Therefore, material that could increase shear strength is required. The aim of this study was to investigate the effect of metal primer and resin cement to shear strength of Ni-Cr alloy in restoration metal coping. This study used 24 cylindrical sample of Ni-Cr alloy with 5 mm diameter and 3 mm height. Sample divided into 4 group, each group consisted of 6 samples. Each sample were sandblasted with Al2O3 50µm in 10 seconds. Two groups were made without application of metal pimer and next two groups were made with application of metal primer. Self-adhesive and adhesive resin cement were used in each group. Shear strength of samples then were tested using Universal Testing Machine (Pearson Panke Equipment Ltd., London). The data were analyzed with two-way ANOVA and continued by LeastSignificantDifference (LSD) test. The results showed that : there was a significant influence between metal primer application and combination of metal primer application and resin cement in shear strength to Ni-Cr alloy (p<0.05), but there was no significant influence of resin cement in shear strength to Ni-Cr alloy (p>0.05). The conclusions of this study were: 1) Metal primer is increasing shear strength of adhesive and self-adhesive resin cement to Ni-Cr alloy. 2) Adhesive resin cement has higher shear strength to Ni-Cr alloy than self-adhesive resin cement.
PERBEDAAN BAHAN IRIGASI AKHIR SALURAN AKAR TERHADAP KEKUATAN PELEKATAN PUSH-OUT BAHAN PENGISI SALURAN AKAR BERBAHAN DASAR RESIN PADA DINDING SALURAN AKAR
Final irrigation is an important factor for adhesion between resin based sealer and root root canal wall. The aim of this study was to evaluate the differences of final irrigant solutions namely 17% EDTA, and 17% EDTA followed by 2% chlorhexidine on push-out bond strength of epoxy sealer and gutta-percha as well as methacrylate sealer and resilon against root canal wall. Twenty eight extracted human mandibular premolars were used in this study. The crowns were cut on cervical region to obtain root length of 14 mm, then the root canals were prepared using crown down technique to apical size # 40/0,04. These specimens were randomly divided into four groups of 7 each teeth. Group IA and IB were irrigated with 17% EDTA, group IIA and IIB were irrigated with 17% EDTA followed by 2% chlorhexidine. The root canals were obturated with epoxy sealer and guttapercha in group IA and IIA. In group I B and II B were obturated with methacrylate sealer and resilon, and then were incubated for 7 days. The specimens were horizontally sectioned into 2mm thick slice, followed by push out test using universal testing machine. All specimens were then observed under a stereo microscope (magnification of 40X) to determine the adherence failure between root canal fillings and dentinal walls. Data were analyzed with a two-way ANOVA at 95% confidence level (α=0,05). A two way ANOVA test showed that push-out bond strength between final irrigant solution of 17% EDTA, and 17% EDTA followed by 2% chlorhexidine were not different (p>0.05). Epoxy sealer and guttapercha produced significantly higher push-out bond strength than methacrylate sealer and resilon (p<0.05), however no interaction occurred between final irrigants and root canal fillings (p>0.05). The failure patterns were dominantly cohesive at the epoxy sealer and gutta percha, whereas adhesive failure at the methacrylate sealer and resilon. It can be concluded that push-out bond strength using final irrigant of 17% EDTA were not different with 17% EDTA followed by 2% chlorhexidine, while epoxy sealer and guttapercha had higher push-out bond strength than methacrylate sealer and resilon.
PENGARUH MODIFIKASI LOKASI PENANAMAN MINI DENTAL IMPLANT TERHADAP RETENSI OVERDENTURE RAHANG BAWAH
Overdenture is removable denture that covered occlusal part of dental implant or natural teeth that have been restored. In patients with alveolar bone resorption, mini dental implant is an option as a support of overdenture. The aim of this study is to determine the difference between the retention force overdenture on mini dental implant position parallel and diagonal. This study was done on the mandibula edentulous model by implanted mini dental implants as a support of overdenture. They used stud mini dental implant (1.8 x 15 mm IMTEC 3M), mini dental implant placed on edentulous models and metal housing on mandibula complete denture. They were implanted in parallel and diagonal position. Then overdenture placed on a tensile test equipment universal testing machine. Each position was given treatment vertical tensile test 16 times. The Student T-test as statistical method was used to analyse with 95% confidence level (α = 0.05). The result showed a significant difference between the position of mini dental implants are implanted parallel and diagonal (p <0.05). The conclusion of this study is that the diagonal position is more retentive than the parallel site.
PENGARUH KOMBINASI LARUTAN IRIGASI SALURAN AKAR DAN JENIS BAHAN BONDING TERHADAP KEBOCORAN MIKRO RESTORASI RESIN KOMPOSIT BULKFILL VISKOSITAS RENDAH PADA DENTIN KAMAR PULPA
Sodium hypochlorite, EDTA and chlorhexidine digluconate are commonly used as root canal irrigants. In irrigation procedure, the irrigant would interact with pulp chamber dentin. Final restoration using bulkfill composite resin will adhere to pulp chamber dentin. Adhesion between composite resin and tooth structure needs bonding agents, either total-etch or self-etch. One of restoration failure is microleakage. The aim of this research was to investigate the influence of root canal irrigants and type of adhesive systems to microleakage of low viscosity bulkfill composite resin on pulp chamber dentin. Thirty-two premolars were divided into four groups (irrigated with NaOCl 2,5%, NaOCl 2,5% and EDTA 17%, NaOCl 2.5%,EDTA 17%, and chlorhexidine 2%, control). Each group was divided into two subgroups (using total-etch and self-etch).Cavitieswere restored with low viscosity bulkfill composite resin, centrifugated, and immersed in 2% methylene blue solution then sectioned longitudinally. Methylene blue penetration was measured with stereomicroscope. Data were statistically analyzed with two-way ANOVA followed by LSD. The results showed that the highest microleakage means was found in NaOCl, EDTA, and chlorhexidine group using total-etch. Based on ANOVA test, combination of root canal irrigants and type of adhesives influence microleakage significantly, however there were no interaction between these variables. It could be concluded that NaOCl-EDTA resulted lower microleakage of low viscosity bulkfill composite resin compared to NaOCl and NaOCl-EDTA-Chlorhexidine.
PENGARUH DESAIN PREPARASI FINISHING LINE DAN SEMEN RESIN TERHADAP KEBOCORAN MIKRO COPING LOGAM GIGI TIRUAN CEKAT
Finishing line chamfer and shoulder is one the marginal adaptation, takes the most important things from the restoration based of porcelain fused to metal.The purpose of this study was evaluate both chamfer, shoulder finishing line and cement resin on microleakage of porcelain fused to metal material fixed bridge. Resin cement is often used as cementing material because it has a bond with the structure ot tooth. Adhesive and sel-adhesive resin cement being in this experiment. This experiment uses twenty- four samples, extracted upper maxilary, which has beeb prapared full-coverage crown beeb diveded into 2 groups chamfer and shoulder. This sample has been prapared into chamfer (I) and shoulder (II), which first chamfer with adhesif (Ia), chamfer self-adhesive (Ib), shoulder adhesive (IIa), shoulder self-adhesive (IIb).These samples have been thermocycled for 25 times then soaked in 2% methylene blue. The data on penetration score using methylene blue in the axial wall of the tooth with 0-3 scale, analyzed by using Kruskal Wallis continued with Mann Whitney method. The result of this study shows significant difference between chamfer and shouder finishing line (p<0.05). There is no significant difference between adhesive and self-adhesive resin cement (p>0.05). Chamfer finishing line with adhesive cementation has the least microleakage. The conclusin : 1) chamfer has less microleakage than shoulder, 2) resin cement has no effect to microleakage.
PERBEDAAN KEBERSIHAN SEPERTIGA APIKAL DINDING SALURAN AKAR DARI RESIDU KALSIUM HIDROKSIDA PASCA PEMBERSIHAN DENGAN TEKNIK IRIGASI MANUAL, SONIK DAN ENDOVAC
Calcium hydroxide is commonly used intracanal medicaments. Calcium hydroxide residue found on the walls of the root canal will have a negative effect on the quality of obturation resulting in leakage and failure of root canal treatment, thus it must be cleaned. This study was conducted to determine differences in the cleanliness of the apical third of the root canal wall of calcium hydroxide residue after irrigation with manual irrigation techniques, sonic and EndoVac. This study uses 27 roots of premolar tooth with a length of 14 mm from the apex. All samples were prepared by the crowndown technique, using calcium hydroxide dressing by mixing with glycerin, then closed using zinc phosphate cement. The samples were stored into the bottle at 37 0 C in incubator for 7 days. The samples were divided into three groups: manual irrigation techniques, sonic and EndoVac. Teeth were split longitudinally. Samples were calculated using version 3 IMAGE RASTER program to determine the surface area of calcium hydroxide residual on the apical third in size ìm² and analyzed using one way ANOVA. The results showed the differences of the three irrigation techniques to residual of calcium hydroxide in the apical third (p <0.05). Residual values of calcium hydroxide lowest in group II (sonic irrigation technique), followed by group III (EndoVac irrigation technique) and the last group I (manual irrigation technique). This study shows the manual irrigation techniques have the lowest ability to clean the calcium hydroxide in the apical third of the root canal walls than sonic and EndoVac, but EndoVac and sonic have the same ability.
EKSPRESI CD10 DAN AKT PADA AMELOBLASTOMA TIPE PLEKSIFORM, FOLIKULER DAN AKANTOMATOSA
Ameloblastoma is a common odontogenic tumor located in jaws, benign but agresif and locally invasive which has high recurrent rate.. There are many histolopathological variant of ameloblastoma. Plexiform, follicular and acanthomatous are the most common type of ameloblastoma according to histopathologycal variant. The aim of study is to make an observation of the differetiation and correlation about CD10 and AKT expression in three different histopathological type of ameloblastoma and to evaluate aggresiveness of them. All the subject were derrived from laboratorium of pathology Dr. Sardjito Hospital, in form of parafin block of ameloblastoma of Prof. Dr. Sardjito Hospital, Yogyakarta, January 2010 until August 2015. There are 23 inclusion samples with three main histopathological be the subjects, each subjects stained by HE, CD10 and AKT, and the immunoscore taken to compare the immunoexpression and correlation between CD10 and AKT in each group. The result of Kruskal-Wallis test reveals that there is a significant difference of CD10 and AKT immunoscore between group of plexiform, follicular and acanthomatous. Mann Whitney test reveals that plexiform CD10 immunoscore higher than follicular, but there is no significant difference CD10 immunoscore between follicular and acanthomatous, and between plexiform and acanthomatous. Mann Whitney test reveals that follicular AKT immunoscore higher than plexiform, and acanthomatous AKT immunoscore higher than plexiform but there is no significant difference AKT immunoscore between follicular and acanthomatous. Rank-Spearman test reveals that there is correlation between CD10 and AKT in each ameloblastoma type. Follicular has stronger coefficient correlation than plexiform, otherwise in acanthomatous type there were a correlation of CD10 and AKT but not significant. Based on statistical analysis all the type of ameloblastoma show aggresivity, but follicullar type more aggresive character than plexiform and acanthomatous