Cover Image

Genetic Variation in Selected Individuals Based on Number of Capsule in M5 Sesame Mutan Lines Detected by RAPD

https://doi.org/10.22146/ipas.32699

Masruru Kholida(1*), Taryono Taryono(2), Rani Agustina Wulandari(3)

(1) Faculty of Agriculture Universitas Gadjah Mada, Yogyakarta
(2) Faculty of Agriculture Universitas Gadjah Mada, Yogyakarta
(3) Faculty of Agriculture Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author

Abstract


Sesame (Sesamum indicum L.) is major oilseed crops with advantages in health and food industry. Due to self pollinated crop, breeding program in sesame utilized gamma rays irradiation to increase genetic variation. The research material consisted of 164 genotypes from 22 selected individual mutant line based on the number of capsules in M5 generation. This study is to detect genetic variation in selected individuals based on number of capsules in M5 generation of sesame mutant line using RAPD markers. The analysis consists of percentage of polymorphic loci, analysis of molecular variance and visualized in cluster and co-ordinate analysis. Fifteen primers RAPD were able to amplified 237 loci. Each genotype in populations had the similarity coefficient of 0.29 – 0.85. Variance within selected individual line (66%) was higher than variance among selected mutant lines (34%). Variance in each selected individual line contributed to its high value. Line 34 showed the lowest polymorphism (23.21%) and line 19 depicted the highest polymorphism (61.60%).  


Keywords


Molecular marker; Polymorphism; Mutation

Full Text:

PDF


References

Abdellatef, E., Sirelkhatem, R., Mohamed Ahmed, M. M., Radwan, K. H. & Khalafalla, M. M. 2008. Study of genetic diversity in Sudanese sesame (Sesamum indicum L.) germplasm using random amplified polymorphic DNA (RAPD) markers. African Journal of Biotechnology 7(24) : 4423-4427

Acquaah, G. 2012. Principles of Plant Genetics and Breeding, 2nd ed. United Kingdom : Wiley-Blackwell Publishing. 740 p.

Aristya, V. E., Taryono, & Wulandari, R. A. 2017. Evaluation of Genetic Parameters in M4 and M5 Generations of Sesame Mutant Lines. SABRAO Journal, 49 (2) : 201 - 210

Bedigian, D. 2010. Cultivated Sesame and Wild Relatives in the Genus Sesamum L. In: Bedigian, D. (Ed.). Sesame The genus Sesamum. Boca Ratoon : CRC Press. 33-77.

Bhat, K. V., Babrekar, P. P., & Lakhanpaul, S. 1999. Study of Genetic Diversity in Indian and Exotic Sesame (Sesamum Indicum L.) Germplasm using Random Amplified Polymorphic (RAPD) markers. Euphytica 110: 21-33

Charlesworth, D. 2003. Effects of inbreeding on the genetic diversity of populations. Phil. Trans. R. Soc. Lond. B. 358:1051-1070.

De Vicente, M. C., Lopez, C. & Fulton, T. 2004. Genetic Diversity Analysis with Molecular Marker Data : Learning Module. International Plant Genetic Resources Institute (IPGRI), Rome, Italy.

Doyle, J.J. & Doyle, J.L. 1990. Isolation of Plant DNA from Fresh Tissue. Focus 12 (1): 13-15

Ercan, A.G., Taskin, M., & Turgut, K. 2004. Analysis of Genetic Diversity in Turkish Sesame (Sesamum indicum L.) Populations using RAPD Markers. Genetic resources & Crop Evolution. 51: 599-607

Forster, B. P. & Shu, Q. Y. 2012. Plant mutagenesis in crop improvement: Basic terms and applications. In: Shu, Q. Y., Forster, B. P. & Nakagawa, H. (Eds.). Plant Mutation Breeding and Biotechnology. CAB International & FAO. 9-20.

Gaafar, R.M., Hamouda, M. & Badr, A. 2016. Seed coat color, weight and eye pattern inheritance in gamma-rays induced cowpea M2-mutant line. Journal of Genetic Engineering and Biotechnology 14 (1) : 61-68

Indriatama, W. M., Trikoesoemaningtyas, Aisyah, S. I. & Human, S. 2016. Pendugaan ragam genetik dan heritabilitas karakter agronomi gandum (Triticum aestivum L.) hasil berbagai perlakuan teknik iradiasi sinar gamma. Jurnal Ilmiah Aplikasi Isotop dan Radiasi 12(2) : 79-88

Kasno, A. & Trustinah. 1998. Pembentukan Varietas Kacang Tunggak. Monograf Balitkabi 3 : 20-58.

Laskar, R. A. & Khan, S. 2017. Assessment on induced genetic variability and divergence in the mutagenized lentil populations of microsperma and macrosperma cultivars developed using physical and chemical mutagenesis. PLoS ONE 12 (9) : 1-18

Laurentin, H. 2009. Data analysis for molecular characterization of plant genetic resources. Genet. Res. Crop Evol. 56: 277-292.

Mangoendidjojo, W. 2003. Dasar-Dasar Pemuliaan Tanaman. Kanisius. Yogyakarta. 182 p.

Poerba, Y. S. & Yuzammi. 2008. Pendugaann keragaman genetik Amorphophallus titanium Becc. Berdasarkan marka Random Amplified Polymorphic DNA. Biodiversitas 9(2): 103-107

Salazar, B., Laurentin, H., Davila, M., & Castillo, M. A. 2006. Reliability of the RAPD technique for germplasm analysis of sesame (Sesamum indicum L.) from Venezuela. Interciencia 31 (6): 456 – 460.

Taryono, Cahyaningrum, P., & Human, S. 2011. The Detection of mutational changes in Sorghum using RAPD. Indonesian Journal of Biotech. 16 (1): 66-70.

Weiss, E. A. 1971. Castor, Sesame, and Safflower. London : Leonard Hill. 311-519

Zainudin, A., Maftuchah, & Fitriani, H. 2014. Analysis of genetic diversity on mutants Jatropha curcas using RAPD. Energy Procedia 47: 1-6



DOI: https://doi.org/10.22146/ipas.32699

Article Metrics

Abstract views : 692 | views : 545

Refbacks

  • There are currently no refbacks.





Ilmu Pertanian (Agricultural Science) ISSN 0126-4214 (print), ISSN 2527-7162 (online) is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Creative Commons License

 

web
analytics View My Stats