Evaluasi Tepung Daun Kelor (Moringa oleifera) yang dihidrolisis Cairan Rumen Domba sebagai Bahan Baku Pakan Ikan Lele (Clarias sp.)

https://doi.org/10.22146/jfs.57468

Achmad Noerkhaerin Putra(1*), Irhas Malik Maula(2), Aryati Aryati(3), Mas Bayu Syamsunarno(4), Mustahal Mustahal(5)

(1) Department of Fisheries, University of Sultan Ageng Tirtayasa, Serang, Banten, Indonesia
(2) Department of Fisheries, University of Sultan Ageng Tirtayasa, Serang, Banten, Indonesia
(3) Department of Fisheries, University of Sultan Ageng Tirtayasa, Serang, Banten, Indonesia
(4) Department of Fisheries, University of Sultan Ageng Tirtayasa, Serang, Banten, Indonesia
(5) Department of Fisheries, University of Sultan Ageng Tirtayasa, Serang, Banten, Indonesia
(*) Corresponding Author

Abstract


Serat kasar tinggi yang terkandung dalam tepung daun kelor (TDK) menjadi penghambat penggunaan TDK sebagai bahan baku pakan ikan lele. Penelitian ini bertujuan untuk mengevaluasi penggunaan TDK yang dihidrolisis dengan cairan rumen domba untuk pakan ikan lele. Uji hidrolisis pada TDK dengan menggunakan 4 dosis cairan rumen yaitu 0, 100, 125, 175 mL/kg dan lama inkubasi selama 0, 12, 24 jam, dilakukan untuk memperoleh dosis dan waktu inkubasi terbaik untuk menurunkan nilai serat kasar dalam TDK. Penelitian terdiri dari tiga pelakuan dan tiga kali ulangan, yaitu pakan referensi/acuan, pakan uji TDK tanpa hidrolis cairan rumen, dan pakan uji TDK dengan hidrolisis cairan rumen. Ikan lele dengan bobot sebesar 5,05±0,005 g dipelihara selama 45 hari dengan padat tebar 15 ekor/wadah. Hasil penelitian menunjukkan bahwa dosis rumen 175 mL/kg dengan lama inkubasi 12 jam menghasilkan nilai serat kasar terkecil (4,33%). Pakan uji TDK dengan hidrolisis cairan rumen menghasilkan nilai kecernaan bahan baku tiga kali lipat lebih besar (50,19%) dibandingkan dengan pakan uji tanpa hidrolisis (16,90%). TDK yang dihidrolisis cairan rumen domba dapat digunakan sebagai bahan baku pakan ikan lele karena meningkatkan kecernaan nutrien, rasio konversi pakan dan pertumbuhan yang lebih tinggi dibandingkan dengan pakan uji TDK tanpa hidrolisis pada pemeliharaan ikan lele.

Keywords


Cairan rumen domba; hidrolisis enzim; ikan lele; tepung daun kelor

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References

Ahmed, M., H. Liang, H. Kasiya, K. Ji, X. Ge, M. Ren, B. Liu, X.  Zhu & J. Sun. 2019. Complete replacement of fish meal by plant protein ingredients with dietary essential amino acids supplementation for juvenile blunt snout bream (Megalobrama amblycephala). Aquaculture Nutrition 25 (1): 205-214

Altan, O. & A.Y. Korkut. 2011. Apperent digestibility pf plant protein based diets by European sea bass Dicentrarchus labrax L., 1758. Turkish Journal Fisheries Aquatic Science 11: 87-92

AOAC (Association of Official Analytical Chemists). 1995. Official Methods of Analysis. 16th ed Volume II: Maryland

Argawal. 2003. Role of protein dynamics in reaction rate enhancement by enzymes. American Chemical, 127(43): 48-56

Bhatnagar, A. & P. Devi. 2013. Water quality guidelines for the management of pond fish culture. International Journal of Environmental Science 3: 1980-2009

Bonvini, E., A. Bonaldo, L. Parma, L. Mandrioli, S. Sirri, M. Grandi, R. Fontanillas, C. Viroli & P.P. Gatta. 2018. Feeding European sea bass with increasing dietary fibre levels: impact on growth, blood biochemistry, gut histology, gut evacuation. Aquaculture 494: 1-9

Budiansyah, A., Resmib, K.G. Wiryawanc, M.T. Soehartonod, Y. Widyastutie & N. Ramlic. 2010. Isolasi dan karakterisasi enzim karbohidrase cairan rumen sapi asal rumah potong hewan. Media Peternakan 33 (1): 36-43

Djissou, A.S.M., C.E. Tossavi, I.N. Odjo, S. Khosio & E.D. Fiogbe. 2020. Use of Moringa oleifera leaves and maggots as protein sources in complete replacement for fish meal I nile tilapia (Oreochromis niloticus) diets. Turkish Journal of Fisheries and Aquatic Sciences 20 (2): 177-183

Duncan, D.B. 1955. Multiple range and multiple ‘F’ tests. Biometrics 11:1-42.

Fitriliyani, I. 2010. Evaluasi nilai nutrisi tepung daun lamtoro gung Leucaena luecophala terhidrolisis dengan ekstrak enzim cairan rumen domba Ovis aries terhadap kinerja pertumbuhan ikan nila Oreochromis niloticus. Jurnal Akuakultur Indonesia 9: 30-37.

Food and Agriculture Organization (FAO). 2016. The state of world fisheries and aquaculture 2016. Contributing to food security and nutrition for all. Rome. 200 pp.

Gatlin, D.M., F.T. Barrows, P. Brown, K. Dabrowski, T.G. Gaylord, R.W. Hardy, W. Herman, G. HU, S. Krogdah, R Nelson, K. Overturf, M. Rust, W. Sealey, D. Skonberg, J. Edward, Souza, D. Stone, R. Wilson & E. Wurtele. 2007. Expanding the utilization of sustainable plant products in aquafeeds: A review. Aquaculture Research 38: 551-579.

Gerile, S. & J. Pirhonen. 2017. Replacement of fish meal with corn gluten meal in feeds for juvenile rainbow trout (Oncorhynchus mykiss). Aquaculture 479: 616-618.

Hansen, A.C. & G.I. Hemre. 2013. Effect of replacing fish meal and oil with plant resources in on-growing diets for Atlantic cod (Gadus morhua L.) Aquaculture Nutrition 19: 641-650.

Hertramp, J.W. & F. Piedad-Pascual. 2000. Handbook on ingredients for aquaculture feed. Kluwer Academic Publisher. Dordrecht, Netherland. pp. 567.  

Huang, F., Wang, L., Zhang, C-X. & Song, K. 2017. Replacement of fishmeal with soybean meal and mineral supplements in diets of Litopenaeus vannamei reared in low-salinity water. Aquaculture 473: 172-180.

Huisman, E.A. 1987. Principles of fish production. Department of Fish Culture and Fisheries, Waganingen Agriculture University, Waganingan, Netherland. 170p.

Jusadi, D., J. Ekasari & A. Kurniansyah. 2013. Peningkatan kualitas kulit buah kakao menggunakan cairan rumen domba untuk pakan ikan nila. Jurnal Akuakultur Indonesia 12 (1): 40-47.

Kamra, D.N. 2005. Special section microbial diversity: rumen microbial ecosystem. Current Science 89 (10):124-135.

Kementrian Kelautan dan Perikanan Republik Indonesia (KKP). 2020. Kelautan dan Perikanan dalam angka. Dikutip dari: https://kkp.go.id/setjen/satudata/page/1453-kelautan-dan-perikanan-dalam-angka.

Kurniasih, T., I. Fitriliyani, I. Melati & Z.I. Azwar. 2012. Pemberian ekstrak enzim kasar dari cairan rumen domba pada tepung bungkil kedelai lokal dan pengaruhnya terhadap pertumbuhan ikan nila. Jurnal Riset Akuakultur 7 (2): 247-256.

Mandalla, N., N.W. Agbo & K. Jauncey. 2013. Evaluation of aqueous extracted moringa Leaf meal as a protein source for nile tilapia juveniles. Tanzania Journal of Agricultural Sciences. 12: 53-64.

Mansour, A.T., L. Miao, C. Espinosa, J.M. Garcia-Beltran, D.C.C. Francisco & M.A. Esteban. 2018. Effect of dietary inclusion of Moringa oleifera leaves on growth and some systemic and mucosal immune parameters of seabream. Fish Physiology and Biochemistry 44: 1233-1240.

Murni & Darmawati. 2016. Optimize the use of liquid rumen in fermentation process on increased the nutrients waste vegetables for tilapia’s feed. International Journal of Oceans and Oceanography 10: 19-28.

Nandakumar, S., K. Ambasankar, S.S.R. Ali, J. Syamadaya & K. Vasagam. 2017. Replacement of Fish Meal with Corn Gluten Meal in Feeds for Asian Seabass (Lates calcarifer). Aquaculture International 25 (1): 1495-1505.

Nates, S.F. 2016. Introduction. In: Nates S., F., editor. Aquafeed Formulation. Academic Press. Oxford, UK. pp xiii-xxii.

National Research Council (NRC). 1993. Nutrient requirement of fish. National Academic Press. Washington DC, USA. 273 pp.

National Research Council (NRC). 2011. Nutrient requirement of fish and shrimp. National Academy Press, Washington DC, USA. 376 pp.

Novriadi, R. 2017. A meta-analysis approach toward fish meal replacement with fermented soybean meal: effects on fish growth performance and feed conversion ratio. Asian Fisheries Science 30: 227-244.

Ogello, E.O., E.M. Kembenya, C.M. Githukia, C.N. Aera, J.M. Munguti & C.S. Nyamweya. 2017. Substitution of fish meal with sunflower seed meal in diets for Nile tilapia (Oreochromis niloticus L.) reared in earthen ponds. Journal of Applied Aquaculture 29 (1): 1-19.

Pamungkas, W. 2012. Koefisien kecernaan fraksi serat bungkil kelapa sawit yang dihidrolisis dengan ezim asal cairan rumen domba sebagai pakan benih ikan patin siam (Pangasius hypopyhalmus). Jurnal Riset Akuakultur 7 (3): 437-445.

Pamungkas, W., D. Jusadi & N.B.P. Utomo. 2011. Uji kecernaan bungkil kelapa sawit yang dihidrolisis dengan enzim cairan rumen domba sebagai pakan benih ikan patin Siam (Pangasius hypopthalmus). Prosiding FITA 2011: 795-800.

Pangentasari, D., M. Setiawati, N.B.P. Utomo & M.T.D. Sunarno. 2018. Komposisi dan nilai kecernaan nutrient tepung daun tarum (Indigofera zollingeriana) yang difermentasi dengan cairan rumen domba pada benih ikan jelawat (Leptobarbus hoevenii (Bleeker, 1851). Jurnal Akuakultur Indonesia 18 (2): 165-173.

Putra, A.N., Jaenudin, F. Sofia, Mustahal, M. Syamsunarno, D. Hermawan & M. Herjayanto, . 2019b. The utilization of vegetable waste silage as feed ingredient in diets for tilapia, Oreochromis niloticus. Proceeding, The 1st International Conference on Agriculture and Rural Development (ICARD).  Serang, 8-9 August 2019. IOP Conference Series: Earth and Environmental Science, Volume 383.

Putra, A.N., W.N. Ningsih, F.S. Nurani, Mustahal & F.R. Indariyanto. 2018. Evaluasi fermentasi daun kelor (Moringa oleifera) sebagai bahan baku pakan ikan nila (Oreochromis niloticus). Jurnal Perikanan dan Kelautan 8 (2): 104-113.

Putra, A.N., A.C. Pradana, D. Novriansyah & Mustahal. 2019a. Effect of dietary fermented lamtoro (Leucaena leucocephala) leaves flour in feed on digestibility and hematological parameter of catfish (Clarias sp.) Jurnal Rekayasa dan teknologi budidaya perairan VIII (1): 951-963.

Samkelisiwe, N.H. & A.G.M. Ngonidzashe. 2014. Replacing fishmeal with kikuyu grass and moringa leaves: Effects on growth, protein digestibility, histological and haematological parameters in Clarias gariepinus. Turkish Journal of Fisheries and Aquatic Sciences 14: 795-806.

Setyoko, H & B. Utami. 2016. Isolasi dan karakterisasi enzim selulase cairan rumen sapi untuk hidrolisis biomassa. Prosiding Seminar Nasional XIII Pendidikan Biologi. Surakarta, 6 Oktober 2016. Universitas Sebelas Maret, 13 (1): 863-867.

Shahzad, M.M., S.M. Hussain, A. Javid & M. Hussain. 2018. Role of phytase supplementation in improving growth parameters and mineral digestibility of Catla catla fingerlings fed moringa by-products based test diet. Turkish Journal of Fisheries and Aquatic Sciences 18: 557-566.

Slawski, H., F. Nagel, K.  Wysujack, D.T. Balke, P. Franz & Schulz. 2013. Total fish meall replacement with canola protein isolate in diets fed to rainbow trout (Oncorhynchus mykiss W.). Aquaculture Nutrition 19 (4): 535-542.

Suprayudi, M.A., S. Alim, I.A. Fauzi, J. Ekasari, M. Setiawati, M.Z. Junior & A.G. Tacon. 2017. Evaluation of hydrolysed rubber seed meal as a dietary protein source for nile tilapia Oreochromis niloticus L. Aquaculture Research 48 (7): 3801-3808.

Suprayudi, M.A., W. Dimahesa, D. Jusadi, M. Setiawati & J. Ekasari. 2011. Suplementasi crude enzim cairan rumen domba pada pakan berbasis nabati dalam memacu pertumbuhan ikan nila (Oreochromis niloticus). Jurnal Iktiologi Indonesia 11 (2): 177-183.

Suprayudi, M.A., W.S. Irawan & N.B.P. Utomo. 2014. Evaluasi tepung bungkil biji karet difermentasi cairan rumen domba pada pakan ikan patin. Jurnal Akuakultur Indonesia 13 (2): 146-151.

Takeuchi, T. 1988. Laboratory work-chemical evaluation of dietary nutrients. In: Watanabe, editor. Fish Nutrition and Mariculture. Kanagawa International Fisheries Training. Japan International Cooperation Agency (JICA), Japan. pp.179-233.

Tan, Q., D. Song, X. Chen, S. Xie & X. Shu. 2018. Replacing fish meal with vegetable protein sources in feed for juvenile red swamp crayfish, Procambarus clarkia: Effects of amino acids supplementation on growth and feed utilization. Aquaculture Nutrition 24 (2): 858-864.

Watanabe, T. 1988. Fish nutrition and mariculture. Departement of Aquatic Bioscience. Tokyo University of Fisheries. JICA, p.79-82.

Weiss, M., A. Rebelein & M.J. Slater. 2020. Lupin Kernel Meal as Fishmeal Replacement in Formulated Feeds for the Whiteleg Shrimp (Litopenaeus vannamei). Aquaculture Nutrition 26 (3): 752-762.

Williams, A.G. & S.E. Withers. 1992. Changes in the rumen microbial population and its activities during the refaunation period after the reintroduction of ciliate protozoa into the rumen of defunated sheep. Canadian J. Microbiology 39: 61-69.

Yusuf, D., M.A. Suprayudi & D. Jusadi. 2016. Peningkatan kualitas pakan ikan nila berbahan tepung bungkil biji karet melalui suplementasi asam amino. Jurnal Akuakultur Indonesia 15 (1): 63-69.

Zhang, C., S. Rahimmejad, Y-R. Wang, K. Lu, K. Song, L. Wang & K. Mai. 2018. Substituting fish meal with soybean meal in diets for Japanese seabass (Lateolabrax japonicas): Effects on growth, digestive enzymes activity, gut histology, and expression of gut inflammatory and transported genes. Aquaculture 483: 172-182.

Zuraida, D. Jusadi & N.B.P. Utomo. 2012. Efektivitas penggunaan enzim cairan rumen domba terhadap penurunan serat kasar dan peningkatan kecernaan bungkil kelapa sebagai pakan ikan nila merah Oreochromis sp. Jurnal Akuakultur Rawa Indonesia 1 (2): 117-126



DOI: https://doi.org/10.22146/jfs.57468

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