LEVEL OF INTERLEUKIN-6 IN OBESE PEOPLE WITH AND WITHOUT INSULIN RESISTANCE

Nedya Safitri; Bambang Sigit Riyanto; H. Ahmad Husain Asdie

doi:10.22146/acta interna.3861

LEVEL OF INTERLEUKIN-6 IN OBESE PEOPLE WITH AND WITHOUT INSULIN RESISTANCE

https://doi.org/10.22146/acta%20interna.3861

Nedya Safitri^(1*), Bambang Sigit Riyanto⁽²⁾, H. Ahmad Husain Asdie⁽³⁾

(1)
(2)
(3)
(*) Corresponding Author

Abstract

ABSTRACT

Background. Obesity is one of the risk factor for type 2 diabetes mellitus characterized by insulin resistance, decrease insulin secretion and hyperglycemia. Chronic inflammation has been proposed to have an important role in the pathogenesis of obesity related insulin resistance. A number of studies have indicated that several humoral markers of inflammation are elevated in people with obesity and type 2 diabetes mellitus, because adipose tissue secretes a number of proinflammatory cytokines, including interleukin-6. The level of plasma IL-6 is increase in obese people.

Objective. To investigate the mean of difference in the level of IL-6 in obese people with and without insulin resistance.

Subjects and Method. The study design was cross-sectional. It was conducted in obese people with BMI ≥ 25 kg/m2. Insulin resistance were measured with HOMA-IR methode, calculated using the following formula: fasting serum glucose X fasting plasma insulin/22,5. Insulin resistance was defined when HOMA-IR > 2,77. Interlukin-6 was measured with Quantikine High Sensitivity human IL-6 ELISA. Difference of mean of IL-6 level was analyzed by student’s t-test for normal distribution and Mann- Whitney U-test if distribution was not normal.

Results. There were 56 subjects, 24 (42,9%) subjects with insulin resistance and 32 (57,1%) subjects without insulin resistance. Obese people with insulin resistance had higher mean level of IL-6 than obese people without insulin resistance, although the difference was not significant (20,05±8,59 vs 18,98±8,15 pg/ml; p=0,639; 95% CI -5,58-3,46).

Conclusion. There was no difference in the mean of IL-6 level in obese people with and without insulin resistance.

Keywords. IL-6, obese, insulin resistance

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References

Suastika, K. 2003. Mekanisme Molekuler Diabesitas Fokus pada Disfungsi sel β. Dalam: S. Adi, S. Murtiwi, A. Tjokroprawiro, Hendromartono, A. Sutjahjo, A. Pranoto (eds.). Naskah Lengkap National Obesity Symphosium II 2003. Surabaya. Perkeni. 9-16.

Saltiel, A.R., & Kahn, C.R. 2001. Insulin Signaling and The Regulation of Glucose and Lipid Metabolism. Nature, 414: 799-809.

Zimmet, B., Alberti, K.G., Shaw, J. 2001. Global and Societal Implications of The Diabetes Epidemic. Nature, 414: 782-7.

Reynolds, K., & He, J. 2005. Epidemiology of Metabolic Syndrome. Am J Med Sci, 330: 273 9.

Hotamisligil, G.S., Arner, P., Caro, J.F., Atkinson, R.L., Spiegelman, B.M. 1995. Increased Adipose Tissue Expression of Tumor Necrosis Factor-α in Human Obesity and Insulin Resistance. J Clin Invest, 95: 2409-15.

Vozarova, B., Weyer, C., Hanson, K., Tataranni, P.A., Bogardus, C., Pratley, R.E. 2001. Circulating Interleukin-6 in Relation to Adiposity, Insulin Action, and Insulin Secretion. Obesity Res, 9 (7): 414-7.

Mohamed-Ali, V., Flower, L., Sethi, J., Hotamisligil, G., Gray, R., Humphries, S.E., York, D.A., Pinkney, J. 2001. β-Adrenergic Regulation of IL-6 Release from Adipose Tissue: In Vivo and in Vitro Studies. J Clin Endocrinol Metab, 86: 5864- 9.

Adam, J.M.F. 2006. Obesitas, Pengertian dan Kriteria Diagnosis. Dalam: J.M.F. Adam (ed.). Obesitas dan Sindroma Metabolik, Bandung. 1-8.

Waspadji, S. 2006. Sindrom Resistensi Insulin dan Prediabetes: Apa yang Harus Dikerjakan. Dalam: Sumaryono, S. Setiati, R. Gustaviani, L. Sukrisman, K.N. Sari, A. Lydia, I.K. Wijaya, P.W. Laksmi (eds.). Pertemuan Ilmiah Tahunan Ilmu Penyakit Dalam 2006. Jakarta. FK-UI. 156-62.

Kern, P.A., Ranganathan, S., Li, C., Ranganathan, G. 2001. Adipose Tissue Tumor Necrosis Factor and Interleukin-6 Expression in Human Obesity and Insulin Resistance. Am J Physiol Endocrinol Metab, 280: E745-E51.

Cefalu, W.T. 2001. Insulin Resistance: Cellular and Clinical Concepts. EBM, 266: 13-26.

Bonora, E., Targher, G., Alberiche, M., Bonadonna, R.C. 2000. Homeostasis Model Assessment Closely Mirror the Glucosa Clamp Technique in the Assessment of Insulin Sensitivity. Diabetes Care, 23 (1): 57-63.

Pedersen, B.K., Steensberg, A., Schjerling, P. 2001. Muscle-derived Interleukin-6: Possible Biological Effects. J Physio, 536 (2): 329-37.

Petersen, A.W., & Pedersen, B.K. 2005. The Anti-Inflammatory Effect of Exercise. J Appl Physio, 98: 1154-64. 23

Royblat, L., Rachinsky, M., Fisher, A., Greemberg, L., Shapira, Y., Douvdevani, A., Gelman, S. 2000. Raised Interleukin-6 Level in Obese Patients. Obesity Res, 8: 673-5.

Real, J.M.F., & Ricart, W. 2003. Insulin Resistance and Chronic Cardiovascular Inflammatory Syndrome. Endocrine Review, 24: 278-301.

Yang, X. Jansson, P., Pelme, F., Laakso, M., Smith, U. 2005. Effect of the Interleukin -6 (-174) G/C Promoter polymorphism on Adiponectin and Insulin Sensitivity. Obesity Res, 13: 813-7.

Real, J.M.F., Broch, M., Vendrell, J., Richart, C., Ricart, W. 2000. Interleukin-6 Gene polymorphism and Lipid Abnormalities in Healthy Subjects. J Clin Endocrinol Metab, 85: 1334-9.

Kiecolt-Glaser, J.K., Preacher, K.J., MacCallum, R.C., Atkinson, C., Malarkey, W.B., Glaser, R. 2003. Chronic Stress and Age-related Increases in the Proinflammatory Cytokine IL-6. PNAS, 15: 9090-5.

Pasic, J., Levy, W.C., Sullivan, M.D. 2003. Cytokines in Depression and Heart Failure. Psychosom Med, 65: 181-93.

Kahl, K.G., Bester, M., Greggersen, W., Rudolf, S., Dibbelt, L., Stockelhuber, K.M., Gehl, H., Sipos, V., Hohagen, F., Scheiger, U. 2005. Viseral Fat Deposition and Insulin Sensitivity in Depressed Women With and Without Comorbid Borderline Personality Disorder. Psychosom Med, 64: 407-12.

Hirosumi, J., Tuncman, G., Chang, L., Gorgun, C.Z., Uysal, K.T., Maeda, K., Karin, M., Hotamisligil, G.S. 2002. A Central Role for JNK in Obesity and Insulin Resistance. Nature, 420: 333-6.

Shoelson, S.E., Lee, J., Goldfine, A.B. 2006. Inflammation and Insulin resistance. J Clin Invest., 116: 1793-801.

McFarlane, S.I., Banerji, M., Sowers, J.R. 2001. Insulin Resistance and Cardiovascular Disease. J Clin Endocrinol Metab, 86 (2): 713-8.

Kershaw, E.E., & Flier, J.S. 2004. Adipose Tissue as an Endocrine Organ. J Clin Endocrinol Metab, 89 (6): 2548-56.

DOI: https://doi.org/10.22146/acta%20interna.3861