Free amino acids profile of honey produced by the Indonesian stingless bee: Tetragonula laeviceps



Agussalim Agussalim(1), Ali Agus(2*), Nurliyani Nurliyani(3), Nafiatul Umami(4)

(1) Faculty of Animal Science Universitas Gadjah Mada
(2) Faculty of Animal Science Universitas Gadjah Mada
(3) Faculty of Animal Science Universitas Gadjah Mada
(4) Faculty of Animal Science Universitas Gadjah Mada
(*) Corresponding Author

Abstract


The objective of this study was to determine free amino acids profile of honey
produced by the Indonesian stingless bee: Tetragonula laeviceps. Honey was obtained from
meliponiculture result in the Ngrandu Katongan Village Nglipar Gunungkidul. The free amino
acids was determined by LC-MS/MS method and the data was analyzed by descriptive
analysis. The results showed that free amino acids content from honey Tetragonula laeviceps
were arginine 591.83, histidine 561.93, lysine 882.03, phenylalanine 232.74, isoleucine 12.34,
leucine 73.55, tyrosine 9.24, methionine 0.29, valine 20.39, proline 60.56, glutamic acid
119.82, aspartic acid 77.31, threonine 45.72, serine 168.65, alanine 62.46, and glycine 60.26
mg/kg while cysteine not detected. It can be concluded that the free amino acids content of
Tetragonula laeviceps honey has 16 free amino acids with large quantity were lysine, arginine,
histidine, phenylalanine, serine and glutamic acid.


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