Production of starter culture to produce biomass of probiotic indigenous lactic acid bacteria



Novi Akhirini(1), Sri Harimurti(2*), Oxsy Puji Astutiningrum(3), Bambang Ariyadi(4), Wihandoyo Wihandoyo(5)

(1) Faculty of Animal Science UGM
(2) Faculty of Animal Science UGM
(3) Faculty of Animal Science UGM
(4) Faculty of Animal Science UGM
(5) Faculty of Animal Science UGM
(*) Corresponding Author

Abstract


The purpose of this study was to compare the mixture of three strains probiotic lactic
acid bacteria (LAB) that would be produced into strarter cultures. The bacteria consisted of:
Lactobacillus murinus Ar-3, Streptococcus thermophilus Kp-2, and Pediococcus acidilactici
Kd-6. The study was conducted by observing the growth of LAB in the glucose yeast peptone
medium for 24 hours. The variables observed were the generation time of three strains of LAB.
Data from the research result analyzed using regression test to see the relationship between
incubation time and optical density value of each bacterium. The result showed that the
generation time of each strain was Lactobacillus. murinus Ar-3 was 3.25 ± 0.006 hours,
Streptococcus thermophilus Kp-2 3.68 ± 0.1 hours, and Pediococcus. acidilactici Kd6 3.74 ±
0.04 hours. The results of the regression analysis showed that the comparison of L. murinus Ar-
3, S. thermophilus Kp-2, P. acidilactici Kd6 in starter culture production was found to be 1,00:
1,18: 1,16 v / v of each strain probiotics. It could be concluded that the generation time of three
strains of probiotic bacteria is different.


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