Biological Value of Papain Synthetic in Layer Ration at Various Protein Contents

Abstract

Two ways of utilizing papain synthetic in layer rations, supplemented directly and
inoculated to soybean oil meal (SBOM-hydrolyzate) before being mixed in the ration, have been studied. Three hundred 24-week old layers were randomly
allocated to 15 dietary treatments to measure the effect of ration containing 17, 15, and 14% CP and two levels of papain (0.075 and 0.15%) either directly supplemented in ration or in form of SBOM-hydrolyzate, derived from inoculating SBOM with 0.5 and 1.0% papain before being mixed in the rations. All dietary treatments factorially (3x5) were plotted in Completely Randomized Design and
replicated to 5. Accumulated data for 4 x 28 days of egg production performances were statistically analyzed with analysis of variance, and followed by DMRT to compared the different effect among single dietary treatments. Results indicated that directly supplemented papain or SBOM-hydrolyzate in the rations produced significantly (P<0.0l) higher and more efficient as compare to dietary control.
However, both parameters statistically were not affected by the levels of papain. The significant interaction effect (P<0.05) between levels of protein and papain treatments were observed on feed intake, hen day production, and egg weight. It was, generally concluded that supplementation papain in the ration produced better perfomiance as compared either with dietary control or diet containing hydrolyzate of SBOM, specially for low protein ( 14%) layer diet.