THE CAPACITATION PATTERN OF BALI BULL SPERMS FILTRATEI) BY SEPHADEX G—200 USING DIFFERENT DILUTERS DURING FREEZING PROCESS

Abstract

The aim of this research was to study the capacitation pattern of Bali bull sperm filtrated by Sephadex G~2OO during freezing process using egg yolk Tris aminomethane and lO% bovine serum in egg yolk TCM 199 as diluters. The result of this research was expected to be basic information about sperm capacitation and then it can be used to select better diluters that prevent premature capacitation and acrosomal reaction of Bali bull sperm in filtrated products. The research used fresh Bali bull semen with 2+ of mass motility and 70% of individual motility. The method of this research was experiment with two experiments using different diluters. The variables measured were percentage of incapacitated, capacitated and acrosomal reaction sperm. The data analyzed by T~test (Paired Comparison). The result showed that filtrated products percentage of incapacitated sperm decreased (P<0,0l), but percentage of capacitated (P<0,0l) and acrosomal reaction sperm (P<0,05) increased on both diluters. During before freezing. percentage of incapacitated sperm decreased (P<0,0l), and then capacitated and acrosomal reaction sperm increased (P<0,05) on both diluters. In post thawing, percentage of incapacitated sperm decreased (P<0,01), but capacitated (P<0,05) and acrosomal reaction sperm increased (P<0,0l) on both diluters. The percentage of incapacitated, capacitated and acrosomal reaction spenn using egg yolk Tris aminomethane and l0% bovine serum in egg yolk TCM 199 diluters in filtrated products were 59,3Zi2,67%; 23,58i2,53%; l7,l1i3,lO% and 6l,38i1,70%; 22,32i3,89%; l6,30i3,09%. In before freezing products were 54,80i2,83%; 25,17il,68%; 20,03i2,30% and 5Z,39d:2,67%; 25,37:t:Z,6'7%; 22,24:t2,6l%. In post thawing were 49,99:e2,37%; 28,73i2,55%; 21,28il1,61% and 45,87-3,12%; 27,82i3,l 6%; 26.3 l:l;3,Z9%. It was concluded that 10% bovine serum in egg yolk TCM 199 diluter was more effective to prevent premature capacitated sperm in filtrated products, but egg yolk Tris aminomethane diluter was more effective to maintain sperm quality in before freezing and post thawing. It is suggested that necessary for further research to examine the real capacitated and acrosomal reaction sperm or membrane degenerative.