ASSOCIATION BETWEEN GENETIC MARKERS AND GROWTH TRAITS IN MERINO AND INDONESIAN THIN TAIL (ITT) BACKCROSS SHEEP



E. T Margawati(1*), K. J. Fullardz(2), Indriawati Indriawati(3), W. Raadsmaz(4)

(1) Research Centre for Biotechnology, Indonesian Institute of Sciences, Jl. Raya Bogor K]\/I 46, Cibinong, INDONESIA
(2) Centre for Advanced Technologies in Animal Genetics and Reproduction, Faculty of Veterinary Sciences, University of Sydney, AUSTRALIA
(3) Research Centre for Biotechnology, Indonesian Institute of Sciences, Jl. Raya Bogor K]\/I 46, Cibinong, INDONESIA
(4) Centre for Advanced Technologies in Animal Genetics and Reproduction, Faculty of Veterinary Sciences, University of Sydney, AUSTRALIA
(*) Corresponding Author

Abstract


The association between markers and quantitative measurements was investigated
to detect quantitative trait loci (QTL) for growth traits in a population of four reference families of paternal half-sib backcross Merino and Indonesian Thin Tail (ITT). A total of 137 microsatellite markers were used in genome-wide scan covering 26 autosomal chromosomes. Performance data was analyzed for all backcross sheep included birth weight, 90-day and 180-day adjusted body weights as growth traits. Statistical and genetic analysis was conducted online using QTL Express. The study indicated the existence of 5 putative QTL located on ovine chromosomes (OAR) of 2, 5, 7, 18 and 23 (birth weight on OAR5, 90-day weight on OAR 2, 7 and l8 and 180-day weight on OAR 18 and 23). Indicated QTL were for birth weight on OAR 5, for 90-day weight on OAR 2, 7 and 18, and for 180-day weight on OAR 18 and 23. The QTL on chromosomes 5, 7 and 18 were the most significant (P5001) based on chromosome-wide permutation testing. The peak F ratio for the QTL region on chromosome 7 was located at 8cM flanked by BM3033 and RNS5/BRN whilst the peak location for QTL on OAR 18 was located at 96cM and flanked by CSSMO18 and TMR1/AKT1 markers. Potential gene identification was carried out through NCBI website, suggesting strong positional candidate genes for growth traits on chromosome 18.


Keywords


Microsatellite Marker; Growth Trait; QTL; Sheep.

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