Effect of Doe Blood Serum Supplementation to Buck Semen on the Head to Head Agglutination Test



Hassan Ishag Harren(1*), Mohamed Abd Elmoneim Salih(2), Abdel Aziz Makkawi(3), Hatim Idris(4)

(1) Faculty of Agriculture, Department of Animal Production, Omdurman Islamic University, Khartoum - Sudan P.O Box 382
(2) Faculty of Agriculture, Department of Animal Production, Omdurman Islamic University, Khartoum - Sudan P.O Box 382
(3) Faculty of Agriculture Studies- Department of Animal Production, Sudan University Of Science and Technology, Khartoum North, Shambat P.O Box: 407
(4) Faculty of Agriculture, Department of Animal Production, Omdurman Islamic University, Khartoum - Sudan P.O Box 382
(*) Corresponding Author

Abstract


The aims of this study was to determine the effect of adding caprine blood serum on the extended semen quality of tow exotic breeds and one local Sudanese breed namely Nubian (local), Saaneen and Shami (exotic). This experiment was conducted in two farms, the rst in Khartoum Center for Improvement Goat Breed (Ministry of Animals Wealth). The second farm in Animal Production Research Centre farm for small ruminants belonging to the Ministry of Animal Wealth. Semen and blood samples from 27 bucks and 27 doe were collected by use (AV). The goat blood serum was added to skimmed milk and egg yolk semen extenders, serum composed 10% of each extender. The effects of the goat blood serum, together with skim milk and egg yolk extenders for the stored semen were evaluated in vitro at temperatures of 5°C. The results demonstrated that the characteristics of 10% serum treated semen extended in egg yolk or skim milk and stored at 5°C was signi cant (P>0.05) effects on all studied traits compared with skim milk or yolk egg extended semen. The sex associated with skim milk and egg yolk in the three breeds on the motility, acrosome integrity and agglutination. Hormonal effect also observed in this study re ected sex in uence on this phenomenon since demonstrating the signi cant effect of estrogen comparing to progesterone. The effect of blood serum and the conservation period on the stored semen are also quite clear in this study. A signi cant (P>0.05) difference showed the Nubian semen maintained better quality during 7 days of conservation compared to Saaneen and Shami semen. 


Keywords


goat breeds, doe blood serum, buck semen, and (HHA) test.

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