Hair Pig Content Identification from Paint Brush using Porcine Detection Kit for Halal Verification

Abstract

Paint brush could be made of any material including pig hair. Some of the brush might be used in cake and bakery home industry. Therefore it is important to identify whether brushes that are sell in market area are made of a small part of hair pig. This research aimed to identify protein content in two kind of brush with L and T trade mark. The brush was cut into small part and then weighed 0.2 gram, then dissolved into 21 mL SDS 2% solution for 30 minutes. The sample than was added 21 mL PBS in pH 7.2 and then be incubation at temperature 61 oC for 12 hours long. Sample than homogenized using magnetic stirrer for an hour at room temperature. The amount of 42 ml of sample added by 42 ml H2SO4 10%. The sample was heated in 40 oC water bath for an hour and was shaked regularly every 1 minutes. Finally sample was added 10 ml of NH4HCO3 10%. The result show that two sample were positively content of protein. To confirm the result, the samples also tested by porcinedetection kit.