Phenotypic And Genotypic F2th and F3th Performances Candidate of The New Breed Ongole Croosed Agrinak Cattle

Abstract

Increasing of domestic beef production, can be done by forming a new breed of beef cattle that are low-quality feed resistant. The aim of this research to know penotypic and genotypic performance (since birth/calf until become cow/bull) of Ongole Croosed (OC) cattle, as parents who will produce candidates of the new breed OC Agrinak cattle that are low-quality feed resistant. The research during two generations (7 years) conducted at experimental stall in Beef Cattle Research Station, used 201 heads OC female and 28 heads OC male as its F2^th, also 328 heads OC female and 29 head OC male as its F3^th. Ration contain low quality nutrient (crude protein 8-9%, crude fiber 22 to 26%, organic matter 85-87%, total digestible nutrients 53 to 55%). Penotipic observation was done by weighing and measuring of cattle, while genotypic observation was done by polymerase chain reaction (PCR) and Sequencing analysis. Parameters observed: weight and hump high at birth, weaning, 12 and 24 months and at first mating ages; also sequence of nucleotide acid growth gene GH891. Phenotypic data is processed and presented descriptively, while genotypic data used Bio edit program. The results showed: weight and hump high at birth, weaning, 12 and 24 months and at first mating ages between F2^th and F3^th were: 24.8±3.4 kg with 68.1±5.6 cm and 25.2±3.4 kg with 71.0±3.8 cm; 113.5±21.6 kg with 100.4±29.2 cm and 126.4±30.7 kg with 104.2±6.1 cm; 140.8±33.0 kg and 105.1±7.3 cm with 157.1±28.1 kg and 107.4±6.3 cm; 206.6±46.7 kg and 117.4±5.1 cm with 227.4±36.3 kg and 128.5±7.8 cm; 252.3±51.2 kg and 130.6±2.7 cm with 274.8±31.9 kg and 131.6±3.8 cm respectively. While SNP results showed there are four major types of nucleotide acid sequences, i.e. 68.3% ACGTCGG; 10.0% ACGATCG; 6.7% ATGCCGG and 6.7% ATGTCGG from population. It was concluded that F3^th performance of new breed OC Agrinak cattle was better than its F2^th, and has four major sequences of SNP in its HG891 gene.