Identification Single Nucleotide Polimorphism of Melanocortin 4 Receptor Gene in Madura Cattle

Abstract

Signaling by the Melanocortin 4 Receptor (MC4R) is important for mediation the effect of leptin on food intake, homeostasis energy which effects to economic traits such as body weight, EBV and carcass weight. MC4R gene plays a key role in the hypotalamic control of food intake and energy balance. The aim of this study was to identify Single Nucleotide Polimorphism (SNP) of Melanocortin 4 Receptor (MC4R) gene in Madura cattle. Twenty seven Madura cattle were used in this study. Primers were designed based on alignment 11 Genbank in both Bos taurus, Bos indicus and Bos grunniens. The forward primer: 5’-GTCGGGCGTCTTGTTCATC-3’and reverse primer: 5’-GCTTGTGTTTAGCATCGCGT-3’ were used to amplify 493 bp of PCR product. Single nucleotide polymorphism was detected by means of DNA sequencing. As a result, this study detected two SNPs of MC4R in exon region (g. 1108 C>T and g. SNP 1133 C>G). In case of g. 1133 C>G was missense mutation and 1108 C>T was silent mutation. SNP g. 1133 C>G was changed of amino acid valin (V) to leucine (L). Based on restriction enzyme mapping, HpyCH4IV can recognize the SNP in region 1133 C/G. The HpyCH4IV enzyme may be used for digesting of the targeted gene using PCR-RFLP method. Next, the SNPs may be used as a marker to be associated with growth traits and feed intake in Madura Cattle in the future study.