Virulent Factors of Sorbitol-Negative Escherichia coli Isolated from Quail

Khusnan Khusnan(1*), Wahyu Prihtiyantoro(2)

(1) Akademi Peternakan Brahmaputra Yogyakarta
(2) Akademi Peternakan Brahmaputra Yogyakarta
(*) Corresponding Author


Colibacillosis in poultry is a disease caused by Avian Pathogenic Escherichia coli (APEC) which can affect a significant economic loss in the poultry industry. APEC strains in this study were characterized based on the hemolysin and hemagglutinin production, as well as detection of four essential virulent genes, including the genes of increased serum survival (iss), capsule antigen (kps), temperature sensitive hemagglutinin (tsh) and enterohemolysin (hly) gene. In this study, all 21 Escherichia coli isolates from quails were negative in the MacConkey Sorbitol test, 38.1% of isolates showed hemolytic and 52.4% hemagglutinin activities. Genotypically, 61.9% of isolates had iss gene, kps gene for 9.5%, hly gene for 9.5%, and 4.8% of isolates had the tsh gene. These isolates are pathogenic strains and potentially acts as zoonotic agents.
Keywords: Escherichia coli, pathogen, quail, sorbitol-negative

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