2024-03-29T14:18:25Z
https://jurnal.ugm.ac.id/ijdr/oai
oai:jurnal.ugm.ac.id:article/65708
2021-05-06T03:46:34Z
ijdr:ART
The Effect of Diabetes Mellitus on the Thickness of Gingival Junctional Epithelium (Study in the Experiment of Caspase-3)
Eljawadi, Abdelaziz
Utoro, Totok
Purwanti, Nunuk
Streptozotocin; diabetes mellitus; junctional epithelium; expression of caspase-3
Diabetes mellitus (DM) is a metabolic disorder manifested by abnormally high levels of blood glucose, resulting in hyperglycemia that affects the oral cavity, leading to periodontitis. The junctional epithelium (JE) is the epithelial component of the dento-gingival unit that is in contact with the toothsurface. Apoptosis and proliferation of JE are essential to maintenance JE thickness. Apoptosis is programmed cell death that can be triggered by various signals and is characterized by well-defined morphologic changes and biochemical features. Caspase-3 is involved in the underlying mechanisms of apoptosis, and the activation of caspase-3 is considered to be the final step in many apoptosis pathways. Purpose of this study was to investigate the effect of DM on the expression of caspase-3 and the thickness of JE. Sixteen male Sprague-Dawley rats were used and divided equally into two groups: the diabetic group that injected intraperitoneal by streptozotocin (STZ) and negative control group. Measurements of blood glucose levels were analyzed before and at 2, 4 weeks after STZ injection. In addition, JE thickness and expression of caspase-3 were examined after 2 and 4 weeks. JE was stained by hematoxylin-eosin (H&E) staining for thickness measurement and the immunohistochemistry by using the anti-caspase-3 antibody for caspase-3 expression measurement and examined under light microscope. The results of the present study showed that a decrease of JE thickness and increase of caspase-3 expression were obtained while increasing the diabetic duration. Two ways Anova and Least Significant Difference (LSD) tests indicated a significant difference of JE thickness and caspase-3 expression between all groups except in diabetic group after 2 and 4 weeks. Also, caspase-3 expression in diabetic group after 2 and 4 weeks (P > 0.05) were not significantly different. It can be concluded that diabetes mellitus (DM) affected on the thickness and caspase-3 expression of JE. Furthermore, the results suggest that high expression of caspase-3 was associated with the diabetes-induced apoptotic cell-death resulting in reduction of JE thickness.
Fakultas Kedokteran Gigi, Universitas Gadjah Mada
2021-05-06
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://jurnal.ugm.ac.id/ijdr/article/view/65708
10.22146/theindjdentres.65708
The Indonesian Journal of Dental Research; Vol 1, No 1 (2012); 1-9
2442-3300
2087-8710
eng
https://jurnal.ugm.ac.id/ijdr/article/view/65708/31213
Copyright (c) 2021 The Indonesian Journal of Dental Research
oai:jurnal.ugm.ac.id:article/65711
2021-05-06T03:46:35Z
ijdr:ART
Influence of Bacterial Endotoxin on Mucosal Immune Response to Phosphorylcholine
Mulyatno, Sapta Adisuka
Kataoka, Kosuke
Fukui, Makoto
Campos, Tselmeg Baatarjav Rita Cristina Orihuela
Ito, Hiro-O
Bacterial lipopolysaccharide (LPS), a component of the outer membrane of gram-negative bacteriathat initiates inflammation by activation innate immune responses through Toll-like receptor 4(TLR4). However, the influence of LPS on the mucosal immune reactions remains to be addressed.This study was examined the effect of LPS in nasal vaccination model. BALB/c and C57BL/6 micewere nasally immunized with keyhole limpet hemocyanin (KLH) conjugated with hapten phosphorylcholine (PC) or trinitrophenol (TNP) with LPS as a mucosal adjuvant, in the presence orabsence of cholera toxin (CT). The antibody titers were measured in serum, saliva, and nasal washfluids by an enzyme-linked immunosorbent assay (ELISA) in IgM, IgG, and IgA isotype-specificmanner. The epitope-specific antibody production induced in blood and mucosal fluid was furtherenhanced by LPS for all isotypes examined. Besides, LPS, which has rarely been regarded as a mucosal adjuvant, was tested for its adjuvanticity by comparing the nasal immunization with PC-KLH plus LPS or with PC-KLH plus CT. LPS showed high adjuvanticity almost equal to CT. Possible differences of LPS from CT as a mucosal adjuvant remains to be elucidated.
Fakultas Kedokteran Gigi, Universitas Gadjah Mada
2021-05-06
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://jurnal.ugm.ac.id/ijdr/article/view/65711
10.22146/theindjdentres.65711
The Indonesian Journal of Dental Research; Vol 1, No 1 (2012); 10-20
2442-3300
2087-8710
eng
https://jurnal.ugm.ac.id/ijdr/article/view/65711/31214
Copyright (c) 2021 The Indonesian Journal of Dental Research
oai:jurnal.ugm.ac.id:article/65713
2021-05-06T03:46:35Z
ijdr:ART
The Effect of Toothbrushing Duration on Nickel Chromium Alloy Wear
Barunawati, Sri Budi
Sunarintyas, Siti
Dharmastiti, Rini
Nickel chromium alloy; abrasion; wear volume; weight loss; surface roughness
Nickel-chromium alloy is a preferred material for fixed partial denture due to its low cost as well as good physical and mechanical properties. Tooth brushing using toothpaste produces abrasion on restoration, especially in a long period. This study aimed to observe the effect of toothbrushing duration on the wear of nickel-chromium alloy. Twenty four specimens of nickel-chromium alloy (Metal 4all, Ivoclar, USA) in 30X15X1mm3 dimension were treated using tooth brushing simulation machine (wear test machine, pin on plate unidirectional movement type) and toothpaste (modification of Balsam formula). The brushing durations were 30.9, 77.25, 123.6, and 154.5 hoursas the simulation of 2, 5, 8, and 10 years tooth brushing. Surface roughness and weight difference as abrasion indicator were measured and analyzed using one-way ANOVA followed by LSD test. Tooth brushing duration of 2, 5, 8, and 10 years increased nickel-chromium alloy surface roughness (Ra) by 0.16, 0.39, 0.43, and 0.56µm with weight loss of 8%, 15%, 23%, and 32 %, respectively. These differences were statistically significant (p <0.05). The result of LSD test showed a significant effect (p <0.05) between groups of toothbrushing duration. The increase of surface roughness affects the increase of wear volume of nickel-chromium alloy indicated by R = 0.11 for brushing duration of 2, 5, 8, and 10 years. The conclusion of this study was 10 years tooth brushing promoted wear on nickelchromium alloy, whichwas indicated by the increase in surface roughness and weight loss.
Fakultas Kedokteran Gigi, Universitas Gadjah Mada
2021-05-06
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://jurnal.ugm.ac.id/ijdr/article/view/65713
10.22146/theindjdentres.65713
The Indonesian Journal of Dental Research; Vol 1, No 1 (2012); 21-28
2442-3300
2087-8710
eng
https://jurnal.ugm.ac.id/ijdr/article/view/65713/31215
Copyright (c) 2021 The Indonesian Journal of Dental Research
oai:jurnal.ugm.ac.id:article/65714
2021-05-06T03:46:36Z
ijdr:ART
Lithium Disilicate Glass-Ceramic Surface Appearance after Acid Surface Treatment
Alrefae, Sana Mohammed
Sunarintyas, Siti
Widowati, Widowati
Hydrofluoric acid; Surface treatment; Concentration; Surface roughness; Lithium disilicate glass-ceramic
Dental ceramics are widely used and studied in dentistry because they are durable, aesthetically appealing and provide excellent biocompatibility. All glass-ceramic surfaces must be etched using hydrofluoric acid (HF) to increase surface roughness determined by roughness average (Ra) before cementation to a tooth surface. This research aimed to analyze the effect of hydrofluoric acid surface treatment concentration on the surface roughness of lithium disilicate glass ceramic. A total of fifteen discs of lithium disilicate glass ceramic were prepared (10mm in diameter and 1mm in thickness). Specimens were divided into 3 groups (n=5). Group A (control) was no treatment, group B was etched by 5% HF for 2 min, and group C was etched by 9.5% HF for 2 min. The etched surfaces were observed by Scanning Electron Microscope (SEM). The measurement of the Ra of the lithium disilicate glass ceramic was determined with surface roughness tester machine. The results showed that the means of Ra (μm) were 0.096±0.009μm, 0.608±0.054μm, and 0.892±0.101μm in group A, B, and C, respectively. The one-way ANOVA showed there was an effect of hydrofluoric acid surface treatment concentration on the surface roughness of the lithium disilicate glass ceramic. The post hoc test showed there was a difference of Ra (μm) among the experimental study groups (p<0.05). In conclusion, the concentration of hydrofluoric acid influences Ra of lithium disilicate glass ceramic.
Fakultas Kedokteran Gigi, Universitas Gadjah Mada
2021-05-06
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://jurnal.ugm.ac.id/ijdr/article/view/65714
10.22146/theindjdentres.65714
The Indonesian Journal of Dental Research; Vol 1, No 1 (2012); 29-35
2442-3300
2087-8710
eng
https://jurnal.ugm.ac.id/ijdr/article/view/65714/31216
Copyright (c) 2021 The Indonesian Journal of Dental Research
oai:jurnal.ugm.ac.id:article/65715
2021-05-06T03:46:36Z
ijdr:ART
Identification of Veillonella spp. on Tongue Plaque and Saliva Using Real-Time PCR
Dhaniarti, Karina
Deandra, Fathia Agzarine
Djais, Ariadna A
Bachtiar, Boy M
Veillonella spp.; real-time PCR; tongue plaque; saliva
Veillonella spp., Gram-negative obligate anaerobic cocci bacteria, amounts to 3% in the oral cavity, relies on the fermentation of lactate as a carbon and energy source for growth. The bacteria are considered anti-cariogenic as they metabolize lactic acid into propionic acid which increases oral environment’s pH and reduces demineralization rate of tooth structure. Identification of Veillonella spp. using traditional methods is difficult due to the lack of conventional phenotypic and biochemical tests. Thus, the biomolecular methods are suitable for the specific detection and identification of Veillonella spp. One of the biomolecular methods that can be used is real-time Polymerase Chain Reaction (PCR), which the results can be qualitative and quantitative. This study aimed to identify Veillonella spp. in tongue plaque’s and saliva’s samples using Real -time PCR. The DNA of Veillonella spp. derived from 36 samples, 18 samples of tongue plaque and 18 samples of saliva, were extracted using a freeze-thaw method and then quantified by real-time PCR using forward primer 5’-CCG TGA TGG GAT GGA AAC TGC-3’ and reverse primer 5’-CCT TCG CCA CTG GTG TTC TTC-3’. Veillonella spp. in 18 samples of tongue plaque was 3,06 x 107 CFU/ml and in 18 saliva samples was 1,51 x 105 CFU/ml. It was concluded real-time PCR can detect Veillonella spp. from all tongue plaque’s and saliva’s samples.
Fakultas Kedokteran Gigi, Universitas Gadjah Mada
2021-05-06
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://jurnal.ugm.ac.id/ijdr/article/view/65715
10.22146/theindjdentres.65715
The Indonesian Journal of Dental Research; Vol 1, No 1 (2012); 36-43
2442-3300
2087-8710
eng
https://jurnal.ugm.ac.id/ijdr/article/view/65715/31217
Copyright (c) 2021 The Indonesian Journal of Dental Research