The Effect of Calcium Hydroxide on Fibroblast Cells Viability

Vincentia Adya Paramitta(1*), Tetiana Haniastuti Heni(2), Susilowati Susilowati(3)

(*) Corresponding Author


Calcium hydroxide [Ca(OH)2] is widely used as medicament in dental pulp and root canal therapy. Previous studies demonstrate the ability of calcium hydroxide to induce necrosis in dental pulp tissue. However, the mechanism of tissue destruction remains unknown.  The aim of this study was to investigate fibroblast cell viability in response to calcium hydroxide exposure. In this study, Vero fibroblast cell line was treated with various concentrations of calcium hydroxide for 24 hours.  Cell viability was measured by using MTT assay. Our results showed significant decrease in cell viability after exposed with calcium hydroxide at concentration 62.5 and 125 µg/ml. The result indicated that calcium hydroxide induced cell death in Vero cell line in a dosedependent manner. This study suggests that fibroblast cell death may involved in the mechanism of pulp tissue necrosis caused by calcium hydroxide


Calcium hydroxide; cell viability

Full Text:



Okiji T. 2002. Pulp as a connective tissue.In: Hargreavers, Goodi HE, Ed. Seltzer and Bender’s Dental Pulp. Chicago: Quintessence Publishing Co.Inc.: 95-122.

Ferreira ANS, Silveira L, Genovese WJ, de Araújo VC, Frigo L, de Masquita RA, Guedes E. 2006. Effect of Gas laser on reactional dentinogenesis induction in human teeth. Photomed Laser Surg


Harty FJ, Ogston R. 1993. Kamus Kedokteran Gigi. Penerbit Buku Kedokteran EGC.

Stanley H. 2002. Calcium hydroxide and vital pulp therapy. In: Hargreavers, Goodi HE, Ed. Seltzer and Bender’s Dental Pulp. Chicago: Quin- tessence Publishing Co.Inc.: 309-24.

Desai S, Chandler N. 2009. Calcium hydroxide based root canal sealers: A Review. J Endodont 35:475-8.

Kopel HM. 1997. The pulp capping procedure in primary teeth “revisited”. ASDC J Dent Child 64: 327-33.

Markovic D, Zivojinovic V, Vucetic M. 2005. Eval- uation of three pulpotomy medicaments in pri- mary teeth. Eur J Paediatr Dent 6: 133-8.

Huth K C, Paschos E, Hajek-Al-Khatar N, Holl- weck R, Crispin A, Hickel R, Folwaczny M. 2005. Effectiveness of 4 pulpotomy techniques – Ran- domized controlled trial. J Dent Res 84:1144-8.

Zurn D, Seale NS. 2008. Light-cured calcium hydroxide vs formocresol in human primary molar pulpotomies: A randomized controlled trial. Pediatr Dent 30: 34-41.

Torabinejad M, Chivian N. 1999. Clinical appli- cations of mineral trioxide aggregate. J Endo- dont 25: 197-205.

Bramante CM, Luna-Cruz LM, Sipert CR, Ber- nadineli N, Garcia RB, de Moraes IG, de Vas- concelos BC. 2008. Alveolar mucosa necrosis induced by utilization of calcium hydroxide as root canal dressing. Int Dent J 58: 81-5.

Piva E, Tarquỉnio SBC, Edmarco FF, Silva AF, de Araứjo VC. 2006. Immunohistochemical expression of fibronectin and tenascin after direct pulp capping with calcium hydroxide. Oral

Surg Oral Med Oral Pathol Oral Radiol Endod 102: e66-e71.

Schmalz G, Schweikl H, Hiller KA. 2000. Release of prostaglandin E2, IL-6 and IL-8 from human oral epithelial culture models after exposure to compounds of dental materials. Eur J Oral Sci

: 442-8.

Pawińska M, Skrzydlewska E. 2003. Release of hydroxyl ions from calcium hydroxide prepara- tions used in endodontic treatment.Rocz Akad Med Bialymst 48: 145-9.

Atthanassiadis B, Abbott PV,Walsh LJ. 2000. The use of calcium hydroxide, antibiotics and biocides as antimicrobial medicaments in endo- dontics. Aust Dent J 52: S64-S78.

Tamburic SD, Vuleta GM, Ognjanovic JM. 1998. “In vitro release of calciumand hydroxyl ions from two types of calcium hydroxide prepara- tions,” Int Endodon J 26:125–30.

Estrela C, Pẻcora JD, Silva RS. 1988. pH analyze of vehicles and calcium hydroxide paste. Braz Endodon J 3: 41-7.

Majima HJ, Oberley TD, Furukawa K, Mattso MP, Yen H, Szweda LI, St.Clair DK. 1998. Prevention of mitochondrial injury by manganese super- oxide dismutase reveals a primary mechanism for alkaline-induced cell death. J Biol Chem 123: 8217-24.

Cobb JP, Hotchkiss RS, Karl IE, Buchman TG. 1996. Mechanisms of cell injury and death. Br J Anaesth 77: 3-10.

Wu H Wen L, Shi J. 2001. Effect of calcium hydroxide on human pulp cell intracellular calcium ion in vitro. Zhonghua Kou Qiang Yi Xue Za Zhi 36: 128-9.

Berridge MJ. 1993. Inositol trisphosphate and calcium signaling. Nature 361: 315–25


Article Metrics

Abstract views : 1316 | views : 1179


  • There are currently no refbacks.





website statistics


View My Stats