Immobilization of Glucose Oxidase on Modified-Carbon-Paste-Electrodes for Microfuel Cell
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| 1. | Title | Title of document | Immobilization of Glucose Oxidase on Modified-Carbon-Paste-Electrodes for Microfuel Cell |
| 2. | Creator | Author's name, affiliation, country | Laksmi Ambarsari; Department of Biochemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Kampus IPB Dramaga, Bogor 16680, West Java; Indonesia |
| 2. | Creator | Author's name, affiliation, country | Inda Setyawati; Department of Biochemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Kampus IPB Dramaga, Bogor 16680, West Java; Indonesia |
| 2. | Creator | Author's name, affiliation, country | Rini Kurniasih; Department of Biochemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Kampus IPB Dramaga, Bogor 16680, West Java; Indonesia |
| 2. | Creator | Author's name, affiliation, country | Popi Asri Kurniatin; Department of Biochemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Kampus IPB Dramaga, Bogor 16680, West Java; Indonesia |
| 2. | Creator | Author's name, affiliation, country | Akhiruddin Maddu; Department of Physics, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Kampus IPB Dramaga, Bogor 16680, West Java; Indonesia |
| 3. | Subject | Discipline(s) | |
| 3. | Subject | Keyword(s) | biosensor; carbon-paste-electrode; glucose oxidase; glutaraldehyde; nano-fiber polyaniline |
| 4. | Description | Abstract | Glucose oxidase (GOx) is being developed for many applications such as an implantable fuel cell, due to its attractive property of operating under physiological conditions. This study reports the functional immobilization of glucose oxidase onto polyaniline-nanofiber-modified-carbon-paste-electrodes (GOx/MCPE) as bioanodes in fuel cell applications. In particular, GOx is immobilized onto the electrode surface via a linker molecule (glutaraldehyde). Polyaniline, synthesized by the interfacial polymerization method, produces a morphological form of nanofibers (100-120 nm) which have good conductivity. The performance of the polyaniline-modified-carbon-paste-electrode (MCPE) was better than the carbon- paste-electrode (CPE) alone. The optimal pH and temperature of the GOx/MCPE were 4.5 (in 100 mM acetate buffer) and 65 °C, respectively. The GOx/MCPE exhibit high catalytic performances (activation energy 16.4 kJ mol-1), have a high affinity for glucose (Km value 37.79 µM) and can have a maximum current (Imax) of 3.95 mA. The sensitivity of the bioelectrode also was high at 57.79 mA mM-1 cm-2. |
| 5. | Publisher | Organizing agency, location | Universitas Gadjah Mada |
| 6. | Contributor | Sponsor(s) | |
| 7. | Date | (YYYY-MM-DD) | 2018-03-15 |
| 8. | Type | Status & genre | Peer-reviewed Article |
| 8. | Type | Type | |
| 9. | Format | File format | Full Text PDF |
| 10. | Identifier | Uniform Resource Identifier | https://jurnal.ugm.ac.id/ijc/article/view/21183 |
| 10. | Identifier | Digital Object Identifier (DOI) | https://doi.org/10.22146/ijc.21183 |
| 11. | Source | Title; vol., no. (year) | Indonesian Journal of Chemistry; Vol 16, No 1 (2016) |
| 12. | Language | English=en | en |
| 13. | Relation | Supp. Files | |
| 14. | Coverage | Geo-spatial location, chronological period, research sample (gender, age, etc.) | |
| 15. | Rights | Copyright and permissions |
Copyright (c) 2016 Indonesian Journal of Chemistry This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. |