Genetic Determination and Clonal Relationships of Staphylococcus aureus Isolated from Dairy Cows in Baturraden, Central Java, Indonesia
https://doi.org/10.22146/ijbiotech.9302 Fatkhanuddin Aziz(1), Siti Isrina Oktavia Salasia(2*), Mitra Slipranata(3)
(1) Graduate School of Biotechnology
(2) Department of Clinical Pathology, Faculty of Veterinary Medicine, Gadjah Mada University, Indonesia
(3) Department of Clinical Pathology, Faculty of Veterinary Medicine, Gadjah Mada University, Indonesia
(*) Corresponding Author
Abstract
Cases of mastitis in cows at Baturraden are leading to signifi cant and ongoing problems due to reduced
production and lower milk quality. This study was designed to identify which of selected virulence determinant
genes of S. aureus are involved in the Baturraden infection, and to determine the clonal relationship among
these isolates. Seventeen isolates were identifi ed as S. aureus based on their biochemical properties and species
specifi city for 23S rRNA and nuc genes. S. aureus isolates were genotypically characterized for the selected
virulence determinants: coa, clfA, fnbA, fnbB, cap5, spa IgG and spa X- region genes. Clonal relationship analysis
among isolates was carried out using AFLP and results compared with previously confi rmed relationships
between selected S. aureus isolated from other regions. The results show that eight isolates contain all the
genes, but six isolates lack fnbB and two isolates lack cap5 genes. AFLP analysis showed that all isolates of S.
aureus originating from cows in Baturraden belong to one cluster. This study provides additional knowledge
about S. aureus infection in Baturraden cows, including the number of virulence determinant genes that may
play a role in pathogenicity.
production and lower milk quality. This study was designed to identify which of selected virulence determinant
genes of S. aureus are involved in the Baturraden infection, and to determine the clonal relationship among
these isolates. Seventeen isolates were identifi ed as S. aureus based on their biochemical properties and species
specifi city for 23S rRNA and nuc genes. S. aureus isolates were genotypically characterized for the selected
virulence determinants: coa, clfA, fnbA, fnbB, cap5, spa IgG and spa X- region genes. Clonal relationship analysis
among isolates was carried out using AFLP and results compared with previously confi rmed relationships
between selected S. aureus isolated from other regions. The results show that eight isolates contain all the
genes, but six isolates lack fnbB and two isolates lack cap5 genes. AFLP analysis showed that all isolates of S.
aureus originating from cows in Baturraden belong to one cluster. This study provides additional knowledge
about S. aureus infection in Baturraden cows, including the number of virulence determinant genes that may
play a role in pathogenicity.
Keywords
Staphylococcus aureus; virulence determinants; dairy cows; PCR; AFLP
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PDF DOI: https://doi.org/10.22146/ijbiotech.9302
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Copyright (c) 2016 Fatkhanuddin Aziz, Siti Isrina Oktavia Salasia, Mitra Slipranata
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