Low pH resistant lactic acid bacteria from cow rumen waste

Ahimsa Kandi Sariri; Kustantinah Kustantinah; Zaenal Bachruddin; Chusnul Hanim

doi:10.22146/ijbiotech.85251

Low pH resistant lactic acid bacteria from cow rumen waste

https://doi.org/10.22146/ijbiotech.85251

Ahimsa Kandi Sariri⁽¹⁾, Kustantinah Kustantinah⁽²⁾, Zaenal Bachruddin⁽³⁾, Chusnul Hanim^(4*)

(1) Animal Science Department, Universitas Veteran Bangun Nusantara, Jl. Sujono Humardani No. 1 Jombor, Sukoharjo, Jawa Tengah, Indonesia
(2) Faculty of Animal Science, Universitas Gadjah Mada, Jl. Fauna No. 03, Karang Gayam, Caturtunggal, Depok, Yogyakarta 55281, Indonesia
(3) Faculty of Animal Science, Universitas Gadjah Mada, Jl. Fauna No. 03, Karang Gayam, Caturtunggal, Depok, Yogyakarta 55281, Indonesia
(4) Faculty of Animal Science, Universitas Gadjah Mada, Jl. Fauna No. 03, Karang Gayam, Caturtunggal, Depok, Yogyakarta 55281, Indonesia
(*) Corresponding Author

Abstract

This study aims to obtain a new strain of lactic acid bacteria (LAB) with the ability to survive at low pH, resulting from isolation, selection, and identification from rumen waste. The research included four stages: isolation of bacteria from rumen waste, selection, and identification. The selection procedure involved growth of LAB in a low pH medium. Molecular identification procedure was conducted using the 16S rRNA gene sequence amplification method with universal primers 27F (AGAGTTTGATCCTGGCT CAG) and 1429R (TAGGGTTACCTTGTTACGACTT). The results of the isolation and identification were analyzed descriptively, revealing that five petri dishes (5a, 10a, 10b, 16a, and 18b) contained lactic acid bacteria which produced clear zones. Among these, isolate 18b was the only LAB strain that survived in a pH 3.5 medium. The results of the molecular identification using the 16s rRNA gene showed that isolate 18b belonged to Limosilactobacillus fermentum.

Keywords

Identification; Isolation; Lactic acid bacteria; Low pH

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DOI: https://doi.org/10.22146/ijbiotech.85251