Purification and Characterization of Streptomyces sp. IK Chitinase

https://doi.org/10.22146/ijbiotech.7820

Sebastian Margino(1*), Agustinus Joko Nugroho(2), Widya Asmara(3)

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(*) Corresponding Author

Abstract


Streptomyces sp. IK isolated from compost inoculants, could produce extra cellular chitinase in a medium containing 0.2% (w/v) colloidal chitin, fermented for 96 hours at 30oC. The enzyme was purified by a combination of ammonium sulphate precipitation and DEAE-Cellulose anion-exchange chromatography. On SDS-polyacrylamide gel electrophoresis analysis, the purified enzyme showed a mass of 71 kDa. Chitinase was optimally active at pH of 6.7 and at 37oC. Km value and Vmax of the protein for colloidal chitin were 2.92 mg/ml and 4.26 ìg/h, respectively.

Key words : chitinase, Streptomyces, purification, characterization

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DOI: https://doi.org/10.22146/ijbiotech.7820

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