Production of Poly-α-hydroxybutyrate (PHB) from Sago Starch by The Native Isolate Bacillus megaterium PSA10

https://doi.org/10.22146/ijbiotech.7804

Nur Arfa Yanti(1*), Langkah Sembiring(2), Sebastian Margino(3),

(1) 
(2) 
(3) 
(*) Corresponding Author

Abstract


A new bacterial strain that produces amylase and poly-a-hidroxybutyrate (PHB) using sago starch as carbon source was characterized and identified to be member of the Bacillus megaterium group based on phenotypic characteristics  and 16S rDNA gene sequences. Amylase activity was determined spectrophotometrically on the basis of substrate concentration reduction. PHB production was quantified with UV spectrophotometer. The polymer produced by B. megaterium PSA10 was identified by  Fourier Transform Infrared spectroscopy (FTIR). The result of the study showed that the amylase specific activity B. megaterium PSA10 was 593,61 DUN/mg protein and PHB production from sago starch was 52,28 % of cell dry weight (CDW). FTIR analysis of the polymer indicated that the strain B.megaterium PSA10 was a potent PHB producer.


Full Text:

PDF



DOI: https://doi.org/10.22146/ijbiotech.7804

Article Metrics

Abstract views : 98 | views : 52

Refbacks

  • There are currently no refbacks.


Copyright (c) 2015 Indonesian Journal of Biotechnology




Indonesian Journal of Biotechnology indexed by:

ISSN 0853-8654 (Print)
ISSN 2089-2241 (Online)

 

Creative Commons License

The Indonesian Journal of Biotechnology, its website, and the articles published therein are licensed under a Creative Commons Attribution-ShareAlike 4.0 International License. UGM logo © Universitas Gadjah Mada.


View Stats:

Details