Production and Extraction Of Antibacterial Bacteriocin from Pediococcus sp. NWD 015

https://doi.org/10.22146/ijbiotech.7566

N. Nofisulastri(1), Zaenal Bachruddin(2), Eni Harmayani(3*)

(1) 
(2) 
(3) 
(*) Corresponding Author

Abstract


objectives were to study the growth pattern of Pediococcus sp. NWD 015 and bacteriocin activity, extraction
and characterization of bacteriocin, and to determine the effect of storage time and temperature on bacteriocin
activity. Results showed that the bacteriocin activity increased during growth and reached the highest activity during
stationary phase. The maximum bacteriocin production reached after incubation of the cell for 12 h at 37oC in TGE
broth and decreased after 96 h incubation. Extraction with adsorbtion-desorbtion method could increased a specific
activity of bacteriocin. Bacteriocin from Pediococcus sp. NWD 015 is inactivated by Proteinase-K; however it is still
active by heat treatment at 121oC for 15 min and over pH 2 – 11. Bacteriocin of Pediococcus sp. NWD 015 was effective
againts Enterococcus faecalis, Staphylococcus aureus, Eschericia coli, Listeria monocytogenes but not against Salmonella
thypimurium. The molecular weight of bacteriocin is 4.95 kDa.
Keywords : Bacteriocins, Pediococcus sp NWD 015.

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DOI: https://doi.org/10.22146/ijbiotech.7566

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