Resistance gene expression in selected Indonesian pigmented rice varieties against infection by Xanthomonas oryzae pv. oryzae

https://doi.org/10.22146/ijbiotech.70445

Nur Elia Nadhira(1), Ali Wafa(2), Wahyu Indra Duwi Fanata(3), Hardian Susilo Addy(4*)

(1) Postgraduate Program of Master of Biotechnology, University of Jember, Jl. Kalimantan Tegalboto No.37, East Java 68121, Indonesia; Division of Biology Molecule and Biotechnology, Center for Development of Advanced Sciences and Technology, University of Jember, Jl. Kalimantan Tegalboto No.37, East Java 68121, Indonesia
(2) Division of Biology Molecule and Biotechnology, Center for Development of Advanced Sciences and Technology, University of Jember, Jl. Kalimantan Tegalboto No.37, East Java 68121, Indonesia; Study Program of Plant Protection, Faculty of Agriculture, University of Jember, Jl. Kalimantan Tegalboto No.37, East Java 68121, Indonesia
(3) Postgraduate Program of Master of Biotechnology, University of Jember, Jl. Kalimantan Tegalboto No.37, East Java 68121, Indonesia; Division of Biology Molecule and Biotechnology, Center for Development of Advanced Sciences and Technology, University of Jember, Jl. Kalimantan Tegalboto No.37, East Java 68121, Indonesia
(4) Study Program of Plant Protection, Faculty of Agriculture, University of Jember, Jl. Kalimantan Tegalboto No.37, East Java 68121, Indonesia; Center for Development of Advanced Science and Technology (CDAST) University of Jember, Jl. Kalimantan Tegalboto No.37, East Java 68121, Indonesia
(*) Corresponding Author

Abstract


Rice (Oryza sativa L.) production is limited by bacterial leaf blight (BLB), caused by Xanthomonas oryzae pv. oryzae (Xoo). For decades, researchers have attempted to control this disease by growing plants with blight‐resistant Xa genes. Genetic resources often vary between rice varieties, and there is little information about the genetic resources of the pigmented rice varieties widely grown in Indonesia and their resistance genes against Xoo. The purpose of this study was to determine the expression of Xa genes in pigmented rice such as Inpari 24 and Cempo Merah (red‐pigmented) along with Hitam Bantul (black‐pigmented) and white rice varieties IR64 and Ciherang, and to evaluate their resistance to BLB. All varieties carried the Xa4, Xa10 and xa13 genes but varied in the Xa1, Xa7 and Xa21 genes. The rice varieties expressed some of these genes only after inoculation with Xoo. Disease assessment categorised the three different pigmented rice varieties as resistant (Ciherang, Cempo Merah and Hitam Bantul), while IR64 (white) and Inpari 24 (red) were moderately resistant. There was no specific pattern of Xa genes possession, quality of expression or resistance level to X. oryzae pv. oryzae. Therefore, when breeding plants, the selection of parental variety must be considered in terms of the possession and expression of Xa genes such as Xa10 as a molecular marker for resistance.


Keywords


Xanthomonas oryzae, Xa genes expression, bacterial leaf blight, pigmented rice

Full Text:

PDF


References

Arshad HMI, Hussain N, Ali S, Khan JA, Saleem K, Barbar MM. 2013. Behavior of Bipolaris oryzae at different temperatures, culture media, fungicides and rice germplasm for resistance. Pak. J. Phytopathol. 25(02):37–42.

Chen S, Lin XH, Xu CG, Zhang Q. 2000. Improvement of bacterial blight resistance of ’Minghui 63’, an elite restorer line of hybrid rice, by molecular marker­assisted selection. Crop Sci. 40(1):239–244. doi:10.2135/cropsci2000.401239x.

Chu Z, Fu B, Yang H, Xu C, Li Z, Sanchez A, Park YJ, Bennetzen JL, Zhang Q, Wang S. 2006a. Targeting xa13, a recessive gene for bacterial blight resistance in rice. Theor. Appl. Genet. 112(3):455–461. doi:10.1007/s00122­005­0145­6.

Chu Z, Yuan M, Yao J, Ge X, Yuan B, Xu C, Li X, Fu B, Li Z, Bennetzen JL, Zhang Q, Wang S. 2006b. Promoter mutations of an essential gene for pollen development result in disease resistance in rice. Genes Dev. 20(10):1250–1255. doi:10.1101/gad.1416306.

Chukwu SC, Rafii MY, Ramlee SI, Ismail SI, Oladosu Y, Okporie E, Onyishi G, Utobo E, Ekwu L, Swaray S, Jalloh M. 2019. Marker­assisted selection and gene pyramiding for resistance to bacterial leaf blight disease of rice (Oryza sativa L.). Biotechnol. Biotechnol. Equip. 33(1):440–455. doi:10.1080/13102818.2019.1584054.

Das G, Patra JK, Baek KH. 2017. Insight into MAS: A molecular tool for development of stress resistant and quality of rice through gene stacking. Front. Plant Sci. 8(June):1–9. doi:10.3389/fpls.2017.00985.

Fatimah, Priyatno T, Fadlillah S, Hermanto, Baroya M, Mahrup, Wawan, Sasongko D, Suryadi Y, Kadir T. 2014. Isolation and disease assessment of Xanthomonas oryzae pv. oryzae from Java Island and pathogenic assay on near isogenic lines with different resistant genes. J. Biologi Indones. 10(237­245):237– 245. doi:10.14203/jbi.v10i2.2104.

Gao L, Fang Z, Zhou J, Li L, Lu L, Li L, Li T, Chen L, Zhang W, Zhai W, Peng H. 2018. Transcriptional insights into the pyramided resistance to rice bacterial blight. Sci. Rep. 8(1):1–11. doi:10.1038/s41598­018­ 29899­1.

González JF, Degrassi G, Devescovi G, De Vleesschauwer D, Höfte M, Myers MP, Venturi V. 2012. A proteomic study of Xanthomonas oryzae pv. oryzae in rice xylem sap. J. Proteomics 75(18):5911–5919. doi:10.1016/j.jprot.2012.07.019.

Gu K, Sangha JS, Li Y, Yin Z. 2008. Highresolution genetic mapping of bacterial blight resistance gene Xa10. Theor. Appl. Genet. 116(2):155– 163. doi:10.1007/s00122­007­0655­5.

Hajira SK, Sundaram RM, Laha GS, Yugander A, Balachandran SM, Viraktamath BC, Sujatha K, Balachiranjeevi CH, Pranathi K, Anila M, Bhaskar S, Abhilash V, Mahadevaswamy HK, Kousik M, Kumar TD, Harika G, Rekha G. 2016. A single­tube, functional marker­based multiplex PCR assay for simultaneous detection of major bacterial blight resistance genes Xa21, xa13 and xa5 in rice. Rice Sci. 23(3):144–151. doi:10.1016/j.rsci.2015.11.004.

He Q, Li D, Zhu Y, Tan M, Zhang D, Lin X. 2006. Fine mapping of Xa2, a bacterial blight resistance gene irice. Mol. Breed. 17(1):1–6. doi:10.1007/s11032­-005­8698­2.

Hu K, Cao J, Zhang J, Xia F, Ke Y, Zhang H, Xie W, Liu H, Cui Y, Cao Y, Sun X, Xiao J, Li X, Zhang Q, Wang S. 2017. Improvement of multiple agronomic traits by a disease resistance gene via cell wall reinforcement. Nat. Plants 3:17009. doi:10.1038/nplants.2017.9.

Ke Y, Hui S, Yuan M. 2017. Xanthomonas oryzae pv. oryzae inoculation and growth rate on rice by leaf clipping method. Bio­Protocol 7(19):1–7. doi:10.21769/bioprotoc.2568.

Korinsak S, Darwell CT, Wanchana S, Praphaisal L, Korinsak S, Thunnom B, Patarapuwadol S, Toojinda T. 2021. Identification of bacterial blight resistance loci in rice (Oryza sativa L.) against diverse Xoo Thai strains by genome­wide association study. Plants 10(3):518. doi:10.3390/plants10030518.

Luo D, Huguet­Tapia JC, Raborn RT, White FF, Brendel VP, Yang B. 2021. The Xa7 resistance gene guards the rice susceptibility gene SWEET14 against exploitation by the bacterial blight pathogen. Plant Commun. 2(3):100164. doi:10.1016/j.xplc.2021.100164.

Mau YS, Markus JE, Shirly, Oematan S, Ndiwa AS, Handoko DD, Nasution A, Makbul K. 2017. Genetic diversity of red and black upland rice accessions from east nusa tenggara, Indonesia as revealed by agromorphological characters. Biodiversitas 18(1):197– 211. doi:10.13057/biodiv/d180127.

Maulana Z, Kuswinanti T, Sennang NR, Syaiful SA. 2014. Genetic diversity of locally rice germplasm from Tana Toraja And Enrekang based on RAPD (Random Amplified Polymorphism DNA) markers. Int. J. Sci. Technol. Res. 3(4):198–202. URL https://www.ijstr.org/final­print/apr2014/Genetic­Di versity­Of­Locally­Rice­Germplasm­From­TanaToraja­And­Enrekang­Based­On­Rapd­random­A mplified­Polymorphism­Dna­Markers.pdf.

Mukhtar Z, Hasnain S. 2017. Transgenic basmati rice transformed with the Xa21 gene shows resistance against bacterial leaf blight. Turk. J. Botany 41(1):1– 10. doi:10.3906/bot­1602­17.

Neelam K, Mahajan R, Gupta V, Bhatia D, Gill BK, Komal R, Lore JS, Mangat GS, Singh K. 2020. Highresolution genetic mapping of a novel bacterial blight resistance gene xa­45(t) identified from Oryza glaberrima and transferred to Oryza sativa. Theor. Appl. Genet. 133(3):689–705. doi:10.1007/s00122­019­ 03501­2.

Porter BW, Chittoor JM, Yano M, Sasaki T, White FF. 2003. Development and mapping of markers linked to the rice bacterial blight resistance gene Xa7. Crop Sci. 43(4):1484–1492. doi:10.2135/cropsci2003.1484.

Rasmiyana, Addy HS, Narulita E. 2019. Detection of genes resistant to bacterial leaf blight in rice cultivars from Situbondo and jember, Indoensia. J. Hama dan Penyakit Tumbuh. Trop. 19(2):127–134. doi:10.23960/j.hptt.219127­134.

Rejeki D, Addy HS, Narulita E. 2021. Partial characterization of bacteriophages from Indonesia and its potency as biocontrol of Xanthomonas oryzae pv. oryzae. Int. J. Agric. Biol. 25(1):75–80. doi:10.17957/IJAB/15.1640.

Ronald P, Song WY, Wang GL, Chen LL, Kim HS, Pi LY, Holsten T, Wang B, Williams C, Zhai WX, Zhu LH, Fauquet C. 2008. The rice disease resistance gene, Xa21 , encodes a receptor kinaselike protein. Rice Sci. III(January):247–253. doi:10.1142/9789812814289_0021.

Sahu A, Das A, Saikia K, Barah P. 2020. Temperature differentially modulates the transcriptome response in Oryza sativa to Xanthomonas oryzae pv. oryzae infection. Genomics 112(6):4842–4852. doi:10.1016/j.ygeno.2020.08.028.

Sambrook J, Russell DW. 2001. Molecular cloning: a laboratory manual (3­volume set). New York: Cold Spring Harbor Laboratory Press. Sombunjitt S, Sriwongchai T, Kuleung C, Hongtrakul V. 2017. Searching for and analysis of bacterial blight resistance genes from Thailand rice germplasm. Agric. Nat. Resour. 51(5):365–375. doi:10.1016/j.anres.2017.11.001.

Tekete C, Cunnac S, Doucouré H, Dembele M, Keita I, Sarra S, Dagno K, Koita O, Verdier V. 2020. Characterization of new races of Xanthomonas oryzae pv. oryzae in Mali informs resistance gene deployment. Phytopathology 110(2):267–277. doi:10.1094/PHYTO­02­19­0070­R.

Wang C, Chen S, Feng A, Su J, Wang W, Feng J, Chen B, Zhang M, Yang J, Zeng L, Zhu X. 2021. Xa7, a small orphan gene harboring promoter trap for AvrXa7, leads to the durable resistance to Xanthomonas oryzae pv. oryzae. Rice 14(1):1–16. doi:10.1186/s12284­ 021­00490­z.

Wang GL, Song WY, Ruan DL, Sideris S, Ronald PC. 1996. The cloned gene, Xa21, confers resistance to multiple Xanthomonas oryzae pv. oryzae isolates in transgenic plants. Mol. Plant­Microbe Interact. 9(9):850–855. doi:10.1094/MPMI­9­0850.

Wang J, Tian D, Gu K, Yang X, Wang L, Zeng X, Yin Z. 2017. Induction of Xa10­like genes in rice cultivar nipponbare confers disease resistance to rice bacterial blight. Mol. Plant­Microbe Interact. 30(6):466–477. doi:10.1094/MPMI­11­16­0229­R.

Yoshimura S, Yamanouchi U, Katayose Y, Toki S, Wang ZX, Kono I, Kurata N, Yano M, Iwata N, Sasaki T. 1998. Expression of Xa1, a bacterial blightresistance gene in rice, is induced by bacterial inpculation. Proc. Natl. Acad. Sci. U. S. A. 95(4):1663– 1668. doi:10.1073/pnas.95.4.1663.

Zeng X, Tian D, Gu K, Zhou Z, Yang X, Luo Y, White FF, Yin Z. 2015. Genetic engineering of the Xa10 promoter for broad­spectrum and durable resistance to Xanthomonas oryzae pv. oryzae. Plant Biotechnol. J. 13:993–1001. doi:10.1111/pbi.12342.



DOI: https://doi.org/10.22146/ijbiotech.70445

Article Metrics

Abstract views : 1724 | views : 1505

Refbacks

  • There are currently no refbacks.


Copyright (c) 2022 The Author(s)

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.