Obtaining of transgenic potato (Solanum tuberosum L.) cultivar IPB CP3 containing LYZ‐C gene resistant to bacterial wilt disease

Pasmawati Pasmawati; Aris Tjahjoleksono; Suharsono Suharsono

doi:10.22146/ijbiotech.61682

Obtaining of transgenic potato (Solanum tuberosum L.) cultivar IPB CP3 containing LYZ‐C gene resistant to bacterial wilt disease

https://doi.org/10.22146/ijbiotech.61682

Pasmawati Pasmawati⁽¹⁾, Aris Tjahjoleksono⁽²⁾, Suharsono Suharsono^(3*)

(1) Graduate School of Biotechnology, IPB University, Bogor 16680; Research Center for Bioresources and Biotechnology, IPB University, Bogor 16680
(2) Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University, Bogor 16680
(3) Graduate School of Biotechnology, IPB University, Bogor 16680; Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University, Bogor 16680; Research Center for Bioresources and Biotechnology, IPB University, Bogor 16680
(*) Corresponding Author

Abstract

Bacterial wilt caused by Ralstonia solanacearum is one of the most important bacterial diseases in potato production. This study aimed to obtain the transgenic potato (Solanum tuberosum L.) cultivar IPB CP3, containing LYZ‐C gene encoding for lysozyme type C, resistant to bacterial disease caused by R. solanacearum. Genetic transformation using Agrobacterium tumefaciens LBA4404 to 124 internode explants resulted in the transformation efficiency of about 47.58% with a regeneration efficiency of approximately 30.51%. Gene integration analysis showed that 16 clones were confirmed as transgenic clones containing the LYZ‐C gene. Analysis of resistance to R. solanacearum of three transgenic clones showed that all three transgenic clones were more resistant than a non‐transgenic one. This result showed that the LYZ‐C gene integrated in the genome of transgenic potato increased the resistance of potato plants to R. solanacearum. We obtained two transgenic clones considered resistant to bacterial wilt disease.

Keywords

Bacterial wilt; genetic transformation; lysozyme; potato; transgenic

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DOI: https://doi.org/10.22146/ijbiotech.61682