Anti-metastatic effect of curcumin analog pentagamaboronon-0-fructose (PGB-0-F) against 4T1 breast cancer cells

https://doi.org/10.22146/ijbiotech.36431

Yogi Ertanto(1), Rohmad Yudi Utomo(2), Riris Istighfari Jenie(3), Ratna Asmah Susidarti(4), Edy Meiyanto(5*)

(1) Master Student of Biotechnology Program, Graduate School, Universitas Gadjah Mada, Jalan Teknika Utara, Yogyakarta 255281, Indonesia ;Army Medical Center, Cililitan, East Jakarta, DKI Jakarta, Indonesia
(2) Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Jalan Sekip Utara, Yogyakata 55281, Indonesia
(3) Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Jalan Sekip Utara, Yogyakata 55281, Indonesia; Departement of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Jalan Sekip Utara, Yogyakata 55281, Indonesia
(4) Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Jalan Sekip Utara, Yogyakata 55281, Indonesia; Departement of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Jalan Sekip Utara, Yogyakata 55281 Indonesia
(5) Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Jalan Sekip Utara, Yogyakata 55281, Indonesia; Departement of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Jalan Sekip Utara, Yogyakata 55281, Indonesia
(*) Corresponding Author

Abstract


Development of chemotherapeutic agent and boron carrying pharmaceutical based on triple negative breast cancer is important due to its metastatic progression. Metastases are often more dangerous than the primary tumor and they are responsible for 90% of all cancer deaths.  The purpose of this study is to explore the anti-metastatic activities of the PGB-0 complex with fructose (PGB-0-F) against 4T1 breast cancer cells. Scratch wound healing assay was performed to determine migration inhibition ability of PGB-0-F, while MMP-9 expression were analysed by gelatin zymography.  The testing of anti-migration activity showed that PGB-0-F inhibited  in 4T1 cells, whereas in gelatin zymography assay showed the suppression of MMP-9 expression. PGB-0-F inhibited closure on 4T1 metastatic breast cancer cells line compared to control. PGB-0-F decreased MMP-9 expression level compared to control. Based these results, PGB-0-F has a potency to be developed as chemotherapeutic agent especially as anti-metastatic agent


Keywords


curcumin analogue, PGB-0-F, 4T1 Cells, migration, MMP-9



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DOI: https://doi.org/10.22146/ijbiotech.36431

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