MicroRNA-21 as a biomarker for ovarian cancer detection


Aprilia Indra Kartika(1*), Siti Nur Chasanah(2), Akbar Satria Fitriawan(3), Dewi Sahfitri Tanjung(4), Addin Trirahmanto(5), Heru Pradjatmo(6), Teguh Aryandono(7), Sofia Mubarika Haryana(8)

(1) Faculty of Nursing and Health Sciences, Universitas Muhammadiyah Semarang, Semarang 50273
(2) Biomedical Sciences Graduate Program, Faculty of Medicine, Universitas Gadjah Mada, Jalan Farmako, Sekip Utara, Yogyakarta 55281
(3) Biomedical Sciences Graduate Program, Faculty of Medicine, Universitas Gadjah Mada, Jalan Farmako, Sekip Utara, Yogyakarta 55281
(4) Biotechnology Graduate Program, Graduate School of Universitas Gadjah Mada, Jalan Teknika Utara, Barek, Yogyakarta 55281
(5) Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Gadjah Mada, Jalan Farmako, Sekip Utara, Yogyakarta 55281
(6) Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Gadjah Mada, Jalan Farmako, Sekip Utara, Yogyakarta 55281
(7) Department of Surgery, Faculty of Medicine, Universitas Gadjah Mada, Jalan Farmako, Sekip Utara, Yogyakarta 55281
(8) Department of Histology and Cellular Biology, Faculty of Medicine, Universitas Gadjah Mada, Jalan Farmako, Sekip Utara, Yogyakarta 55281
(*) Corresponding Author


Ovarian cancer is a lethal disease. One of the problems faced by patients with ovarian cancer is the lack of symptoms in its early stages, which results in it only being detected when it is at an advanced stage. Therefore, there is an urgent need for biomarkers that can predict ovarian cancer precisely. The purpose of this study was to determine the expression of microRNA-21 as a predictive biomarker candidate in both early- and advanced-stage ovarian cancer. This was a cross-sectional study using the blood plasma of 21 healthy control subjects and 37 blood plasma samples from patients with ovarian cancer. Blood plasmas were collected, from which the RNA was isolated. Based on the RNA, the cDNA was synthesized and run through qPCR, the results of which were analyzed using the Livak method. The results showed an upregulation of microRNA-21 in the advanced stage by 2.14 fold compared with the early stage, and 6.13 fold compared with the healthy controls (p < 0.05). The upregulation of microRNA-21 in early-stage ovarian cancer was 2.86 fold compared with the healthy control subjects (p < 0.05). In addition, there was an increase in the expression of microRNA-21 in ovarian cancer by 4.14 fold compared with the healthy controls (p < 0.05). Based on these results, it can be concluded that the expression of microRNA 21 upregulated with the severity of the disease.


microRNA-21; minimally invasive; ovarian cancer; predictive biomarker

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Kartika et al.


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DOI: https://doi.org/10.22146/ijbiotech.35692

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