The effect of ethanolic extract of black and white rice bran (Oryza sativa L.) on cancer cells

https://doi.org/10.22146/ijbiotech.26814

Rizal Maarif Rukmana, Nyoman Puniawati Soesilo, Rumiyati Rumiyati, Rarastoeti Pratiwi,

(1) Departement of Biology, Faculty of Biology, Universitas Gadjah Mada, Jl.Teknika Selatan, Sekip Utara,Yogyakarta 55281, Indonesia
(2) Faculty of Biology, Universitas Gadjah Mada, Jl.Teknika Selatan, Sekip Utara,Yogyakarta 55281, Indonesia
(3) Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara Yogyakarta 55281, Indonesia
(4) Faculty of Biology, Universitas Gadjah Mada, Jl.Teknika Selatan, Sekip Utara,Yogyakarta 55281, Indonesia

Abstract


Indonesia has a wide range of rice cultivars and pigments. This rice can be used as a source of phytochemical compounds for cancer prevention. This research aims to analyze the cytotoxic activities of the ethanolic extract of black rice bran of 4 local cultivars i.e. ‘Cempo Ireng’, ‘Woja Laka’, ‘Toraja’ and ‘IR­64’ (white rice) on  cancer cells and to determine the compounds groups of those extracts. First step, rice bran was extracted with ethanol. This extract was applied to Raji (a human Burkitt Lymphoma cancer), HepG2 (a human liver cancer), and Vero (a nonhuman cell line) cells in order to measure the cytotoxic activities by using MTT assay. To determine descriptively the compounds groups of phenolics, flavonoids, terpenoids, steroids, and alkaloids the thin layer chromatography method was performed. The IC50 value was analyzed quantitatively by using probit analysis. Results showed that the IC50 values of ethanolic extract of rice bran ‘Woja Laka’, ‘Toraja’, ‘Cempo Ireng’ and ‘IR 64’ on HepG2 cells were 857.23±99.19; 1,896.55±83,8; 1,494.47±87.81 and 727.89±145,97 µg/ml respectively. The IC50 on Raji cells were 816.61±85.31; 1,079.93±28.31; 1,627.82; ±119.82, and 769.33±61.43 µg/ml respectively. The IC50 on Vero cells were 1,295.2±37; 1,232.07±165.51; 1,874.14±169.56, and 724.4±122.79 µg/ml respectively. The ethanolic extracts of rice bran from four cultivars contain phenolics, flavonoids, terpenoids, and steroids. However, alkaloids could not be detected. The variety of rice cultivars indicates the variation of cytotoxic activities on cancer cells. The ethanolic extracts of rice bran from those four rice cultivars contain similar kinds of organic compounds groups but vary in the Rf values.


Keywords


cancer cells; compound group; cytotoxicity; ethanolic extract and rice bran

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References

Amalia, A. R. 2015. Effect of methanolic and water extract fractions of black rice bran (Oryza sativa l.) ‘Cempo Ireng’ on apoptotic induction and cell cycle profile of T47D and WiDr cell lines. Tesis. Fakultas Biologi. UGM: Yogyakarta.

Banjerdpongchai, R., Wudtiwai. B. and Sringarm, K. 2013. The cytotoxic effect of purple rice extracts on human cancer cells related to their active compounds. Research. Gate. Conference Paper. ASEAN Food Conference.

Chaudhary, R.C. 2003. Specialty rices of the world : Effect of WTIO and IPR on its production trend and marketing. J. Food. Agric. Env., 1 (2), 34 -41.

Chen, M, H., and Bergman, C.J. 2005. A rapid procedure for analysing rice bran tocopherol, tocotrienol and g-oryzanol contents. J. Food. Comp. Anal., 18, 139–151.

Forster, G.M., Raina, K., Kumar, A., Kumar, S., Agarwal, R., Chen. M., Bauer. J. E., McClung, A. M., and Ryan, E. P. 2013. Rice Varietal Differences in Bioactive Bran Components for Inhibition of Colorectal Cancer Cell Growth. J. Food. Chem.,141,1545-1552.

Gujjeti, R, P., and Mamidala, E. 2013. Phytochemical Screening and Thin Layer Chromatographic Studies Of Aerva Lanata Root Extract. Inter. J. Innov. Res. Sci. Eng. Tech.,2, 5725-5730.

Hanahan, D., and Weinberg, R.A. 2011. The Hallmarks of Cancer, Cell 100, 57-70. Hui, C., Bin, Y., Xiaoping, Y., Long, Y., Chunye, C. and Mantian. 2010. Anticancer Activities of an Anthocyanin-Rich Extract From Black Rice Against Breast Cancer Cells In Vitro and In Vivo. J. Nut. Cancer., 62 (8), 1128-1136.

Kanawapee, J., Sanitchon, J., Srihaban, P., and Theerakulpisut, P. 2011. Genetic diversity analysis of rice cultivars (Oryza sativa L.) differing in salinity tolerance based on RAPD and SSR markers Elec. J. Biotech., 14, 0717-3458.

Meiyanto, E., Jenie, R.I., Rahmi, F., and Septisetyani, E.P. 2005. Aktivitas Antikanker Minyak Buah Merah terhadap Sel Kanker Plasma Darah, Sel Kanker Payudara, dan Sel Kanker Leher Rahim. Final Report. Gadjah Mada University.

Bernard T. Wahyu Wiryanta. Meyer, B. N., Ferrigni, N, R., Putnam, J, E., Jacobsen, L, B., Nichols, D, E., McLauglin, J.L. 1982. Brine Shrimp: A Convinient General Bioassay for Active Plant Constituent. Planta Med., 45, 31-34.

Moko, E. M., Purnomo, H., Kusnadi, J. and Ijong, F. G. 2014. Phytochemical content and antioxidant properties of colored and non colored varieties of rice bran from Minahasa, North Sulawesi, Indonesia. Inter. Food. Res. J., 21 (3), 1053-1059.

Phetpornpaisan, P., Tippayawat, P., Jay, M., Sutthanut, K. 2014. A local Thai Cultivar Glutinous Black Rice Bran: A Source of Functional Compounds in immunomodulation, Cell Viability and Collagen synthesis, and Matrix metalloproteinase-2 and -9 Inhibition. J. Funct. Food.,7, 650-661.

Pranatami, D. A. 2016. Cytotocix activity and apoptotic induction of ethanol extract and fractions of black rice bran ‘wojolaka’ against colon cancer cell (WiDr). Tesis. Fakultas Biologi. UGM: Yogyakarta.

Settharaksa, S., Madaka, F., Sueree, L., Chankana, N., Chakree, K., Charoenchai, L. 2008. Cytotoxic activity and Antioxidant Potentials of Cold Pressed Rice Bran Oil. Inter. J. Pharm. Tech. Res., Vol.6, No.2, 686-691.

Sutharut, J., and Sudarat, J. 2012. Total anthocyanin content and antioxidant activity of germinated colored rice. Inter. Food. Res. J., 19(1), 215-221.



DOI: https://doi.org/10.22146/ijbiotech.26814

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