Assessment of genetic diversity among surian Toona sinensis Roem in progenies test using random amplified polymorphic DNA markers

Jayusman Jayusman; Muhammad Na’iem; Sapto Indrioko; Eko Bhakti Hardiyanto; ILG Nurcahyaningsih

doi:10.22146/ijbiotech.25798

Assessment of genetic diversity among surian Toona sinensis Roem in progenies test using random amplified polymorphic DNA markers

https://doi.org/10.22146/ijbiotech.25798

Jayusman Jayusman^(1*), Muhammad Na’iem⁽²⁾, Sapto Indrioko⁽³⁾, Eko Bhakti Hardiyanto⁽⁴⁾, ILG Nurcahyaningsih⁽⁵⁾

(1) Centre for Forest Biotechnology and Tree Improvement Research and Development, Jalan Palagan Tentara Pelajar Km. 15, Purwobinangun, Pakem, Sleman, Yogyakarta 55582, Indonesia
(2)
(3) Faculty of Forestry, Universitas Gadjah Mada, Jalan Agro No. 1, Bulaksumur, Yogyakarta 55281, Indonesia
(4) Faculty of Forestry, Universitas Gadjah Mada, Jalan Agro No. 1, Bulaksumur, Yogyakarta 55281, Indonesia
(5) Centre for Forest Biotechnology and Tree Improvement Research and Development, Jalan Palagan Tentara Pelajar Km. 15, Purwobinangun, Pakem, Sleman, Yogyakarta 55582, Indonesia
(*) Corresponding Author

Abstract

Surian Toona sinensis Roem is one of the most widely planted species in Indonesia. This study aimed to estimate the genetic diversity between a number of surian populations in a progeny test using RAPD markers, with the goal of proposing management strategies for a surian breeding program. Ninety-six individual trees from 8 populations of surian were chosen as samples for analysis. Eleven polymorphic primers (OP-B3, OP-B4, OP-B10, OP-H3, OP-Y6, OP-Y7, OP-Y8, OP-Y10, OP-Y11, OP-Y14, and OP-06) producing reproducible bands were analyzed for the 96 trees, with six trees per family sampled. Data were analyzed using GenAlEx 6.3, NTSYS 2.02. The observed percentage of polymorphic loci ranged from 18.2% to 50%. The mean level of genetic diversity among the surian populations was considered to be moderate (He 0.304). Cluster analysis grouped the genotypes into two main clusters, at similarity levels of 0.68 and 0.46. The first two axes of the PCoA explained 46.16% and 25.54% of the total variation, respectively. The grouping of samples into clusters and subclusters did not correspond with family and their distances, but the grouping was in line with the genetic distances of the samples.

Keywords

genetic diversity; progeny test; RAPD; Toona sinensis

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DOI: https://doi.org/10.22146/ijbiotech.25798