Isolation and caracterization of ficin enzyme from Ficus septica Burm F stem latex

Sri Wahyuni; R. Susanti; Retno Sri Iswari

doi:10.22146/ijbiotech.24200

Isolation and caracterization of ficin enzyme from Ficus septica Burm F stem latex

https://doi.org/10.22146/ijbiotech.24200

Sri Wahyuni⁽¹⁾, R. Susanti^(2*), Retno Sri Iswari⁽³⁾

(1) Biology Department of Mathematic and Science Faculty, Universitas Negeri Semarang, Semarang, Indonesia
(2) Biology Department of Mathematic and Science Faculty, Universitas Negeri Semarang, Semarang, Indonesia
(3) Biology Department of Mathematic and Science Faculty, Universitas Negeri Semarang, Semarang, Indonesia
(*) Corresponding Author

Abstract

This research aims to isolate and characterize the fcin enzyme from Ficus septica stem latex. Ficin from Ficus septica stem latex was isolated using column chromatography. Then enzyme activity was tested at different temperature (40oC, 50oC, 60oC, 70oC) and pH (6.0, 7.0, 8.0) levels. Ficin enzyme activity of joint treatment with variations in temperature and pH was analyzed using two-way ANOVA with a factorial pattern followed by Least Signifcant Difference (LSD) test. The results showed that temperature treatment signifcantly affects enzyme activity. However, the treatment of pH and the interaction between temperature and pH did not signifcantly affect the fcin enzyme activity. There was no signifcant difference in fcin activity at the incubation temperatures of 40oC and 50oC, as well as 60oC and 70oC. However, comparing the incubation temperatures of 40oC and 50oC with treatment 60°C and 70°C showed a signifcant difference in fcin enzyme activity. In the treatment of incubation at pH 6, 7 and 8 for fcin enzyme activity showed no signifcant difference. We concluded that the Ficus septica plant latex contained fcin enzyme with an optimum temperature of 60°C and optimum pH of 6, 7, and 8.

Keywords

Ficus septica Burm F; ficin enzyme; temperature; pH

Full Text:

PDF

References

Gagaoua, M., Boucherba, N.,Darenfed, A.B.,Ziane, F.,Rabah, S.N.,Hafd, K. and Boudechicha, H.R. 2014. Three-phase partitioning as an effcient method for the purifcation and recovery of fcin from mediterranean fg (Ficuscarica L. Latex). Separation and Purifcation Technology 132: 461-467.

Hames, B.D. and Hooper, N.M. 2000. Biochemistry: the instant notes. Hongkong: Springer Verlag.

Hasibuan, P.R.M., Alviyulita, M. and Hanum, F. 2014. Pengaruh penambahan natrium klorida (NaCl) dan waktu perendaman buffer fosfat terhadap perolehan crude papain dari daun pepaya (Carica papaya L.).Jurnal Teknik Kimia 3: 3. 39-44.

Herdyastuti, N. 2006. Isolasi dan karakterisasi ekstrak kasar enzim bromelin dari Batang nanas (Ananascomusus L.merr).Berk. Penelitian Hayati. 12: 75-77.

Iswanto, K.N., Sudarminto and Saparianti, E. 2009. Karakteristik aktivitas proteolitik enzim papain kasar (kajian zat pengaktif dan suhu pengeringan). [Laporan Penelitian]. Malang: Universitas Brawijaya Malang.

Layne, E. 1957. Spectrophotometric and turbidimetric methods for measuring proteins. Methods in Enzimology 3: 447-455.

Masfufatun. 2009.Isolasi dan karakterisasi enzim selulase. http://fk.uwks.ac.id/ archive/jurnal/vol. [23 April 2015]. Ngili, Y. 2010. Biokimia dasar. Bandung: Rekayasa Sains.

Perello, M., Arribere, M.C.,Caffni, N.O. and Priolo, N.S. 2000. Proteolytic enzimes from the latex of FicusPumila L. (Moraceae). Acta Farm Bonaerense 19 (4): 257-262.

Pourmorad, F.,Honary, S., Azadbakht, M., Asgarirad, H. and Golmohammadzadeh, G. 2011. Separation of proteolytic components of Iran ficus carica latex by column chromatography and electrophoresis and latex anticorn activity. International Journal of Pharmaceutical Sciences Review and Reasearch 1(7): 83-85.

Safriani, N., Basyamfar, R.A., and Bachtiar. 2011. Pengaruh konsentrasi ekstrak kasar enzim fsin (crude fcin) dan lama fermentasi terhadap rendemen dan kualitas minyak kelapa. Jurnal Teknologi dan Pertanian Indonesia 3(3): 12-19.

Sajuthi, D., Suparto, I., Yanti and Praira, W. 2010. Purifkasi dan pencirian enzim protease fbrinolitik dari ekstrak jamur merang. Makara Sains 14(2): 145-150.

Sebayang, F. 2006. Imobilisasi enzim papain dari getah pepaya dengan alginat. Jurnal Komunikasi Penelitian 18(2): 34-38.

Siota, L.F. and Villa, T.G. 2011. Native and biotechnology engineered plant proteases with industrial aplications. Food Biopress Tecnol. 4: 1066-1088.

Soeka, Y.S., Rahayu, S.H., Setianingrum, N. and Naiola, E. 2011. Kemampuan bacillus licheniformis dalam memproduksi enzim protease yang bersifat alkalin dan termofilik. Media Litbang Kesehatan
21(2): 89-95.

Walti, A. 1938. Crystalin fcin. J. Am. Chem. Soc. 40: 493.

Wardani, A.K. and Nindita, L.O. 2012. Purifkasi dan karakterisasi protease dari bakteri hasil isolasi dari Whey tahu. Jurnal Teknologi Pertanian 13(3): 149-156.

Witono, Y., Aulanni’am, Subagio, A. and Wijanarko, S.B. 2006. Pemurnian parsial enzim protease dari getah tanaman biduri (Calotropisgigantea) menggunakan ammonium sulphat. Jurnal Teknologi
Pertanian 7(1): 20-26.

DOI: https://doi.org/10.22146/ijbiotech.24200