High Resolution Microsatellite Marker Analysis of Some Rice Landraces Using Metaphor Agarose Gel Electrophoresis

https://doi.org/10.22146/ijbiotech.15269

K. Kristamtini(1*), T. Taryono(2), Panjisakti Basunanda(3), Rudi Hari Murti(4)

(1) Assesment Institute of Agriculture Technology, Yogyakarta, Indonesia
(2) Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia
(3) Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia
(4) Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia
(*) Corresponding Author

Abstract


Microsatellite markers or simple sequences repeats are DNA - based molecular techniques that are
used to see the different among accessions and inbred lines. There are three methods to analysis the results of
the polymerase chain reaction of microsatellite markers namely polyacrylamide gel electrophoresis (PAGE),
capillary electroforesis, and Metaphor Agarose Gel Electroforesis (MAGE), and the Use of MAGE assessed
more easily and economically the polymorphic pattern of DNA markers. This study aimed to obtain fast,
effective and efficient in term of easy and cheap technique to identify microsatellite markers of some black
rice cultivars and F
2 populations from crosses between black with white rice. The results showed that MAGE
successfully separated clearly SSRs alleles with different sizes of less than 25 bp .

Keywords


microsatellite markers; Metaphor Agarose Gel Electrophoresis (MAGE); and rice cultivar

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DOI: https://doi.org/10.22146/ijbiotech.15269

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