Application of Molecular Biology for Identification of Virus Resistance Gene in Melon

https://doi.org/10.22146/ijbiotech.15265

Budi Setiadi Daryono(1*)

(1) Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia
(*) Corresponding Author

Abstract


Source of resistance to an Indonesia isolate of Cucumber mosaic virus (CMV-B2) in melon cultivar
Yamatouri has been reported. Moreover, Creb-2, a locus that confers resistance to CMV-B2 in Yamatouri has
been determined as a single dominant gene. To elucidate the resistance mechanism conferred by Creb-2 in
more detail, it is necessary to clone the Creb-2 gene and determine its molecular structure. One approach is
by amplification and cloning of melon resistance gene analogs (MRGAs) based on degenerated PCR primers
designed from conserved amino acids in the NBS-LRR motifs (P-loop, Kinase-2, and the GLPL) and Toll/
Interleukin-1 receptor-like region (TIR). This study was aimed to identify and characterize the resistance gene
analogs from Cucumis melo L. cv. Yamatouri by employing polymerase chain reactions (PCR) as a molecular
biology tools with degenerate primers based on conserved motifs of cloned R genes. The application of molecular
biology such as DNA isolation, degenerate primers and PCR condition, cloning, sequencing, linkage analysis
and mapping of resistance gene analogs to Creb-2 gene in melon will be widely discussed in this paper

Keywords


resistance gene analogs; Cucumber mosaic virus; Cucumis melo L.

Full Text:

PDF



DOI: https://doi.org/10.22146/ijbiotech.15265

Article Metrics

Abstract views : 478 | views : 338

Refbacks

  • There are currently no refbacks.


Copyright (c) 2016 Budi Setiadi Daryono

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Past issues



The Indonesian Journal of Biotechnology (print ISSN 0853-8654; online ISSN 2089-2241) is published by the Research Center for Biotechnology in collaboration with the Graduate School of Universitas Gadjah Mada. The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License, and attributable to Siti Nurleily Marliana and Joaquim Baeta. Built on the Public Knowledge Project's OJS 2.4.8.1 and designed by Joaquim Baeta. Web
Analytics View website statistics.