2024-03-29T02:18:40Z
https://jurnal.ugm.ac.id/bik/oai
oai:jurnal.ugm.ac.id:article/2980
2019-11-12T01:28:45Z
bik:ART
The influence of stigma and depression on quality of life of leprosy patients
Agnes S. Siswati, Jenny MC Siagian, Carla R Marchira
Background: Stigma attached to leprosy-affected individuals is one of the strongest stigma of diseases. Depression is often occurred to many lepers. Stigma and depression are two factors which affect the quality of life of the lepers. Objective: To determine whether stigma and depression affect the quality of life of leprosy patients in Dermatovenereology outpatient clinic at RSUP Dr Sardjito Hospital. Methods: It was a quantitative and cross-sectional research. The quantitative analysis was conducted with chi-square and multiple regression methods. Result: There was no significant correlation between stigma and quality of life of leprosy patients. The factors which significantly correlated with quality of life were gender, income, and depression score (p < 0.051. Depression score significantly affected the quality of life (B 1.876; P < 0.05; CI 1.166-36.5661. Conclusion: Depression affected the quality of life more than deformity and demographic factors among leprosy patients in Dermatovenereology outpatient clinic at RSUP Dr Sardjito Yogyakarta. Key words: stigma - depression - lepro
Universitas Gadjah Mada
2015-11-18
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/2980
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 41, No 01 (2009)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 41, No 01 (2009)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/2980/pdf
Copyright (c) 2015 Jenny MC Siagian, Carla R Marchira Agnes S. Siswati
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/2982
2019-06-17T07:29:19Z
bik:ART
Standardization of Immunocytochemical method for the diagnosis of Dengue Viral Infection in Aedes aegypti Linn Mosquitoes (Diptera Culicidane)
Umniyati, Sitti Rahmah
methods for virus detection in the mosquito, such as the direct fluorescent-antibody test on head squashes. However, it has the disadvantages of being labor-intensive and requiring fluorescent microscope as well as cryofreezer. Newer methods involving enzyme conjugates such as peroxidase in conjunction with either polyclonal or monoclonal antibodies are greatly improved. With new methods of immunocytochemistry, it is now possible to detect dengue viral antigen in a variety of tissues. Objective: This study was aimed to standardize an immunocytochemical streptavidin-biotin-peroxidasecomplex assay for diagnosis of dengue infection in Aedes aegypti using monoclonal antibody DSSC7. Methods: The infected mosquitoes were held in small cylindrical cages covered with mosquito netting, and incubated at 27:t 1°C ami at relative humidity of 88:t 6 %. The specificity of the immunocytochemical procedure was validated by negative and positive controls showing that the antibody was bound to an appropriate structure. The sensitivity and specificity were also evaluated based on Herrmann's Formula. The presence of dengue antigen on head squash preparation was detected based on ISBPC assay using monoclonal antibody against dengue. The validity and reliability of the measurement were evaluated based on kappa values, according to Landis and Koch. Result: Positive result was detected as discrete brownish granular deposits throughout most visual fields of brain tissue. Dengue viral antigen was immunolocalized to the cytoplasm of brain cells. The immunocytochemical test under light microscope at magnification of 400x was 86.67% sensitive, 96.00% specific, and the kappa value is 0.64. Meanwhile the kappa value between two observers was 0.92, with sensitivity and specificity of 96% and 97% respectively at magnification of 1000x. Conclusion: The monoclonal antibody DSSC7 was sensitive, specific, valid, and reliable as primary antibody to detect dengue viral infection in Ae. aegypti head squash preparation based on immunocytochemical streptavidin-biotin-peroxidase-complex assay under light microscope. Key words: antigen - denguevirus - Aedes aegypti - immunocytochemistry - monoclonal antibody DSSC7
Universitas Gadjah Mada
2015-11-18
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/2982
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 41, No 01 (2009)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 41, No 01 (2009)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/2982/pdf
Copyright (c) 2015 Sitti Rahmah Umniyati
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/4082
2019-10-07T08:08:11Z
bik:ART
Risk factors of constipation in children
Dyah Kurniati, Dyah Kurniati
Background: Despite it is not considered as a dangerous situation in children older than 2 years old, but attention is important in young infant. Improper treatment in constipation may contributed to the raise of anxiety, emotionally disorder either for the children or its relatives.Objective: To determine risk factors related to constipation in children.Design and Methods: A case control study was conducted for all of children who were admitted to ambulatory polyclinic and inpatient clinic of Sardjito Hospital during January 2001 - December 2001. A questionnaire was obtained by the researcher or assistant by direct interview to parent or guardian.Results: We enrolled 86 children, consisted of 43 children in constipation group and the43 children as control. There was significant difference (p < 0.01) between two groups in the presence of the symp-toms (abdominal pain, pain during defecation, vomit, decrease of appetite and encopresis). More childrenin constipation group and significant difference (p<0.01) when reported having toilet training distur- bance, daily fluid intake, the variety of daily meals (vegetables, fruits) and sweetened foods. Relative risk of having constipation due to toilet training disturbance in constipations group is 0.14 (CI: 0.06 - 0.38).Conclusion: Toilet training disturbance, daily fluid intake, the variability of daily meals and sweetened foods are risk factors of constipation in childhood.Key words: children - constipation - clinical findings- risk factors
Universitas Gadjah Mada
2015-11-18
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/4082
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 35, No 4 (2003)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 35, No 4 (2003)
2356-3931
ind
https://jurnal.ugm.ac.id/bik/article/view/4082/3356
Copyright (c) 2015 Dyah Kurniati Dyah Kurniati
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/4083
2019-10-07T08:08:11Z
bik:ART
Death: clinical and forensic anthropological perspectives
Etty Indriati, Etty Indriati
All biological living beings inevitably die, and the ways to die vary although in essence death is a manifestation of the absence of Oxygen in the brain. After death, biological remains undertake proteolysis and decomposition. The aim of this article is to discuss clinical death, cerebral or medicolegal death, social death, phases of cerebral death, and biological process after death—which is important for forensic medicine and forensic anthropology. How long a person die, if the time elapsed is in minutes, hours, days and a week,-it is within the field of medical forensic and pathological forensic. If the time elapsed after death is in weeks, months, or years, it is within the field of forensic anthropology. The time elapsed from biological death to the examination of the death is called postmortem interval. Five case studies on postmortem interval are presented in this article. It can be concluded that knowledge of the biological process of death and the fate of biological remains after death are important for handling abnormal death both in forensic medicine and forensic anthropology.Key words: clinical death, cerebral death, postmortem interval, forensic medicine, forensic anthropology
Universitas Gadjah Mada
2015-11-18
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/4083
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 35, No 4 (2003)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 35, No 4 (2003)
2356-3931
ind
https://jurnal.ugm.ac.id/bik/article/view/4083/3361
Copyright (c) 2015 Etty Indriati Etty Indriati
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/4086
2019-10-07T08:08:11Z
bik:ART
Identification and test of active protein resemble Ribosomr-inactivating proteins (RIPs) on Kaemferia rotunda Linn
Wiryatun Lestariana, Wiryatun Lestariana
Background: Statistically data of US Mortality showed that percentage cause of the death of the cancer is in second place after the heart diseases. Indonesia, Java especially Daerah Istimewa Yogyakarta, the data of The Dr. Sardjito hospital showed that the patients of cancer was increasing in the last year. Indonesian, especially Javanese, uses white turmeric to prevent and to treat cancer. There are some varieties of turmeric available commercially such as Curcuma (Cl mangga Val & Jijp, C. zeodaria and Kaemferia (K) rotunda Linn. The studies showed that C. mangga contain proteins compound that resembles Ribosome-inactivating proteins (RIPs) which have activity to cleave supercoiled DNA. In vitro study indicated that the addition of crude extract of C. mangga on cancer cell-lines (B-LCL, EBV cells and Raji cell-lines) and normal lymphocytes, the percentage of cytotoxic effect on those cancer cell-lines were higher significantly than normal cells.Objective: The aim of the study was to know the presence of RIPs activity in K. rotunda Linn by the ability of the RIPs in cleaving the supercoiled DNA.Methods: The rhizomes of K. rotunda Linn that was in part dried at 40°C. Both wet and dried rhizomes are pounded and then extracted. The resulting crude extract was precipitated to obtain its protein fraction. The crude extract and protein with various concentrations were incubated with the supercoiled DNA and agarose gel electrophoresis was used to test its activity in cleaving the supercoiled DNA. The activity test was done by observing 3 criterions, viz. the thinning of the supercoiled DNA, the circular band thickening and the appearance of the linear band which were subsequently compared to the plasmid DNA without treatment.Result: The results showed that both crude extracts and proteins of both wet and dried samples were able to cleave supercoiled DNA into circular and linear form. The increasing concentrations of the crude extract and the protein resulted in increasing the activity which was indicated by the thickening of circular band and the appearance of the linear band .Conclusion: Kaemferia rotunda contains proteins compound that resemble Ribosome-inactivating proteins (RIPs).which have ability to cleave supercoiled DNA to be circular and linear DNA.Key words: ribosome-inactivating proteins (RIPs) - supercoiled DNA - circular DNA - linear DNA - Kaemferia rotunda Linn
Universitas Gadjah Mada
2015-11-18
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/4086
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 35, No 4 (2003)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 35, No 4 (2003)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/4086/pdf
Copyright (c) 2015 Wiryatun Lestariana Wiryatun Lestariana
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/4087
2019-10-07T08:12:11Z
bik:ART
The effect of 2% salicylic acid as a support of 10% benzoil peroxide gel for mild to moderate acne vulgaris.
Rahajeng Musy, Rahajeng Musy
Background: Cleansing is necessary before the application of topical therapy for acne vulgaris. However ordinary soap leads to dryness. Two percent salicylic acid has comedolytic, anti inflammation and moisturizing activity.Objective: The aim of this study was to assess the effectiveness of 2% salicylic acid soap as a support of 10% benzoil peroxide gel for mild to moderate acne vulgaris.Material and method: The design of this study was randomized, double blind, clinical trial. Fifty twofemales with mild to moderate acne vulgaris with the mean of the age was 18.9 ± 3.06 years old were divided into 2 groups. The first group was treated with 2% salicylic acid soap and the second group was treated with soap base, in addition to 10% benzoil peroxide gel for both groups. The clinical assessment was conducted by counting the acne lesion at 2, 4, 8, and 12 week. While the level of moisture and sebum of the skin was measured by corneometer and sebumeter of Courage – Khazaka at 4, 8, and 12 week. The difference between 2 groups was analyzed statistically by t-test, chi-square test and Mann-Whitney U test. The difference was considered to be significant if p < 0.05.Result: There was significant reduction of the open comedone in salicylic acid soap group
Universitas Gadjah Mada
2015-11-18
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/4087
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 35, No 4 (2003)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 35, No 4 (2003)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/4087/pdf
Copyright (c) 2015 Rahajeng Musy Rahajeng Musy
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/4088
2019-10-07T08:08:11Z
bik:ART
Profile of patients diagnosed as sepsis (ICD X: A41.9) in the Internal Medicine Ward Sardjito Hospital in 2002
Yanri Wijayanti Subroto, Yanri Wijayanti Subroto
Background: Sardjito Hospital as the top referral hospital for the Yogyakarta Special Province and the southern part of Central Java has a mortality rate of 56.83% of patients diagnosed as sepsis (ICD X A41.9). The overall hospital sepsis case in the year 2002 was 275, with 50.54% (139) of which was treated in the Internal Medicine ward. Out of the 139 cases, 44.60% was man and 55.40% was woman. Method: This study was focused on the identification of demographic and clinical factors of patients diagnosed as sepsis (ICD X A41.9) in Internal. Medicine ward Sardjito Hospital in 2002. Demographic data include sex, age, system of hospital admission (referral or self admition. Clinical data include the concomitant disease(s), causes of death, and type of cultured microorganism(s). The identification of these factors may be beneficial for designing an early warning system for fatal cases of patients with sepsisResults: Only 120 (86.33%) out of 139 medical records were eligible for further analysis. Seventy-one (59.17%) patients died in the hospital, whereas 49 (40.83%) were discharged from the hospital, but mostly were forced leave (only 6 patients were discharged in a good condition). The median ± SD age was 53.5 ± 15.99 years old with the youngest age was 17 and the oldest was 98. More than 50% of patients were above 50 years old. As many as 72 (60%) patients were admitted to the hospital referred by other hospitals (a few numbers were referred by private physicians), and only 48 (40%) patients were self-admitted. The average length of hospitalization was 6.96 ± 5.43 days, with the range of 1-25 days. The cause of death of these patients were mostly septic shock (61.97%), followed by respiratory failure (19.72%), intracranial process (14%), cardiac arrest (2.8%) and MOFS (1.4%). The underlying/concomitant diseases are:. gastrohepatology (mostly hepatic cirrhosis and hepatocellular carcinoma); hematology (acute leukemia, multiple myeloma), pulmonology (TB, COPD, pneumonia), oncology (Ca mammae, Ca cervix); endocrinology (DM type 2 with ulcus diabeticum), nephrology (chronic renal failure), cardiology (congestive heart failure), obsgyn (septic puerpuralis) and trop-med (urinary tract infection, malaria), etc.Conclusion: We report here research on medical record of patients diagnosed as sepsis (ICD X: A41.9) in Internal Medicine ward Dr. Sardjito Hospital in 2002. There were 139 cases with only 120 were eligible for analysis. Mortality reached 59.17% of cases with median of age 53.5 years and average length of hospitalization of 6.96 days. The most common cause of death was septic shock and gastrohepatology as the most common comorbid disease.Key words: sepsis-demographic-clinical presentation-cause of death
Universitas Gadjah Mada
2015-11-18
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/4088
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 35, No 4 (2003)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 35, No 4 (2003)
2356-3931
ind
https://jurnal.ugm.ac.id/bik/article/view/4088/3367
Copyright (c) 2015 Yanri Wijayanti Subroto Yanri Wijayanti Subroto
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/6377
2019-05-21T07:15:38Z
bik:ART
Ephaptic crosstalk in Painful Diabetic Neuropathy: an electrodiagnostic study.
Asmedi, Ahmad
Wibowo, Samekto
Meliala, Lucas
Painful diabetic neuropathy – NCS – ephaptic– Diabetes Mellitus
Painful Diabetic Neuropathy (PDN) is a common complication of diabetes mellitus(DM) which significantly causes pain and distress in patients. Release of factors fromdegenerating fibers activating adjacent fibers to produce ephaptic crosstalk have beenproposed as one of the pain mechanism in PDN. Here we aim to detect ephaptic crosstalkbetween small fibers and large fibers in PDN subjects by comparing the electrodiagnosticresult of patients with PDN and patients without PDN.This study used cohort prospective design. Patients with type 2 DM or impairedglucose tolerance (IGT) without PDN from several health facilities in Yogyakarta werefollowed for 12 months for the occurrence of PDN. Demographic, clinical, laboratory andelectrodiagnostic data from all patients were collected and analyzed.One hundred and forty-one subjects (58 men, 83 women) with an average age of 51years (range, 40–61 years), were enrolled in this study. After 48 weeks of observation,12 subjects were found to have PDN. The differences of distal latency between PDNand non-PDN group were significant when measured in median sensory nerve (4.47 ms±2.43 versus 3.39 ms ±1.79, p = 0.002), tibial motor nerve (6.96 ms ±3.07 versus5.90 ms ±2.17, p = 0.041), and sural sensory nerve (6.02 ms ±3.56 versus 3.55ms ±2.90, p <0.001). Among all parameters measured in this study, the H-reflex hadhigher abnormality persentage compared to other electrodiagnostic variable (H latency =30%, H amplitude = 71%, H/M Ratio = 88%, and H-M IPL = 15%).Our result shows that small fiber neuropathy in PDN can be detected by electrodiagnosticstudy which measures large fibers function. This indicates that ephaptic crosstalkbetween small fiber and large fiber happens in PDN.
Universitas Gadjah Mada
2018-04-10
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/6377
10.19106/JMedSci005002201806
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 2 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 2 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/6377/pdf
Copyright (c) 2018 Ahmad Asmedi, Samekto Wibowo, Lucas Meliala
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/6480
2019-11-14T07:32:11Z
bik:ART
Prognostic factors for heart valve surgery outcomes in Dr Sardjito General Hospital Yogyakarta, Indonesia
Priyatno, Wahyu
Supomo, .
Heart valve disease is a serious health probem due its significant effect on mortality.Surgical intervention plays an importan roleinthe management of moderate to the severe valvular heart disease. The evaluation of the patientoutcomes has been widely accepted as one of the important steps to improve the quality of patient care.Recognition of post operative complications might significantly affect the patient quality of life.Therefore, the identification of prognostic factors and morbidity of heart surgery patients can provide valuable insights on improving the quality of patient care.This study aimedto investigat the prognostic factors for heart valve surgery outcomes in Dr. Sardjito General Hospital, Yogyakarta, Indonesia. This study was conducted using data ofpatient’s medical records who underwent heart valve surgeryincluded morbidities and their prognostic factors.The data were analyzed using Chi-square test and logistic regression with significance level of 0.05. During 2010 to 2014, 82 patients underwent heart valve surgeries at the hospital. The kidney disorders and heart failure were significantly associated with the patient morbidity (p<0.05),while the gender, pulmonary disease, angina, and impaired ventricular function were not (p>0.05). Multivariate analysis showed that the heart failure is the most significant factor of morbidity. The risk of New York Heart Association (NYHA) class 3 patients were 7.18 times compared with NYHA class 2 patients. In conclusion, the heart failure is the most significant factor for the morbidityof heart valve surgery followed by the kidney disorders at the Dr. Sardjito General Hospital.
Universitas Gadjah Mada
2017-02-28
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/6480
10.19106/JMedSci005102201908
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 51, No 2 (2019); 159-163
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 51, No 2 (2019); 159-163
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/6480/pdf
Copyright (c) 2017 Wahyu Priyatno, . Supomo
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/11419
2019-08-05T07:20:12Z
bik:ART
The frequency of DISC1 Leu607Phe gene polymorphism in schizophrenia patients at Dr. Soetomo General Hospital Surabaya
Prabowo, gwenny Ichsan
Maramis, Margarita Maria
Yulianti, Erikavitri
Zulaikah, Afrina
Syulthoni, Zain Budi
Margono, Hendy Muagiri
Handajani, Retno
Schizophrenia; DISC1 Leu607Phe gene; polymorphism; PANSS; predisposition
Psychiatry
Schizophrenia is a common health problem in the world, including in Indonesia.Polymorphism of gene disrupted in schizophrenia 1 (DISC1) Leu607Phe is allegedly relatedto the predisposition to schizophrenia. However, studies on the relationship betweenpolymorphism of DISC1 Leu607Phe and schizophrenia in various etnics provided differentresults. The purpose of this study was to determine the frequency of DISC1 Leu607Phegenepolymorphism and its association with treatment response in patients with schizophreniaat Department of Psychiatry, Dr. Soetomo General Hospital Surabaya. In this study, thenumber of male patients with schizophrenia was more than that of the female patients.The mean age of male patients with schizophenia was lower than that of the femalepatients. Schizophrenia patients were primarily came from Javanese ethnic with positiveand negative symptom score (PANSS) lower in male patients than that in the femalepatients. In conclusion, no DISC1 gene polymorphism at codon 607 is observed inschizophrenia patients at Dr. Soetomo General Hospital Surabaya, but the G nucleotidevariation at the number 196.339 in intron regions is found instead.
Universitas Gadjah Mada
2018-03-26
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/11419
10.19106/JMedSci005001201802
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 1 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 1 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/11419/pdf
Copyright (c) 2018 gwenny Ichsan Prabowo, Margarita Maria Maramis, Erikavitri Yulianti, Afrina Zulaikah, Zain Budi Syulthoni, Hendy Muagiri Margono, Retno Handajani
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/11997
2018-12-20T03:00:34Z
bik:ART
Severity and treatment level of acute gastroenteritis with rotavirus in children under 5 years in INDONESIA
Gdara, Fatma Othman
At Thobari, Jarir
Soenarto, Yati
rotavirus - acute gastroentrities - treatment level - vesikari score - children
Rotavirus diarrhea causing gastroenteritis in children under five years is an importantissue that urgently needs to be addressed globally. Delay in management of rotavirusdiarrhea can be fatal. Diagnostic tool for detecting rotavirus is, therefore, needed.However, until now the gold standard diagnostic tools are expensive, often not availableand affordable in health care settings. The aim of the study was to compare the Vesikariclinical severity score of rotavirus-positive with rotavirus-negative in hospitalized childrenwith acute gastroenteritis. Furthermore, the difference of the level of treatment betweenrotavirus-positive with rotavirus-negative was also evaluated. This was a cross sectionalstudy that using secondary data from medical records of five general teaching hospital inIndonesia. Subjects were children aged <5 years with acute watery diarrhea admitted tothe hospital. Statistical analysis used was chi square test, U-Mann Whitney, and KruskalWallis. The results showed that the patient with rotavirus positive have higher dehydration(80.2%) compared to rotavirus negative (70%). The severity level of clinical feature washigher in diarrhea due to rotavirus positive than non rotavirus (11.47± 2.89 vs 10.41± 2.70; p<0.000). The level of treatment was higher in rotavirus positive. The majorityhad treatment plan C (47.7%) higher than plan B and A (45.6% and 30.9%; p<0.050).This was opposite with patient with rotavirus negative that majority had treatment inplan A (69.1%) higher than plan B and C (54.4% and 52.3%) (p<0.001). In conclusion,the severity of gastroentrities in children under 5 years using vesikari score are higher indiarrhea due to rotavirus positive than non rotavirus. The treatment level plan C is higherthan plan B and A in diarrhea due to rotavirus. This is opposite with non rotavirus majorityhave treatment in plan A higher than plan B and C.
Universitas Gadjah Mada
2018-04-04
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/11997
10.19106/JMedSci005001201812
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 1 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 1 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/11997/pdf
10.19106/10.19106/JMedSci005001201812
Copyright (c) 2018 Fatma Othman Gdara, Jarir At Thobari, Yati Soenarto
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/12055
2018-06-19T00:03:59Z
bik:ART
Comparing P-Selectin (CD62P) expression in patients receiving non-leukodepleted vs leukodepleted thrombocyte concentrates
Triyono, Teguh
Mulyono, Budi
Sutaryo, .
Sofro, Abdul Salam
thrombocyte conentrate – transfusion – CD62P expression - thrombocytopenia – adverse effect
Thrombocyte concentrate (TC) transfusion is an important supportive therapy in patients with thrombocytopenia. The risks in platelet transfusions may be related to the content of TC including the contaminant leukocytes. The aim of this study was to assess the risk of increased level of P-Selectin (CD62P) expression of non-leukodepleted TC transfusions.This was a quasi-experimental study. Subjects were children patients aged 1-18 years who received a non-leukodepleted or a leukodepleted TC transfusions. Comparison of the proportion of increased expression of CD62P in both groups expressed as relative risk. The subjects consisted of 51 patients who received non-leukodepleted and 52 patients who received leukodepleted TC transfusions. The risk of increased expression of CD62P in patients receiving non-leukodepleted TC transfusions were 2.38 (95%CI:1.60-3.53) times higher than those who received leukodepleted TC. Non-leukodepleted have significant higher risks of increased CD62P expression than leukodepleted TC transfusions.
Universitas Gadjah Mada
2017-07-19
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/12055
10.19106/JMedSci004903201704
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 49, No 3 (2017)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 49, No 3 (2017)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/12055/pdf_1
Copyright (c) 2017 Teguh Triyono, Budi Mulyono, . Sutaryo, Abdul Salam Sofro
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/12351
2019-10-31T02:47:45Z
bik:ART
Patient prognosis after relief of obstruction
Danarto, R
obstructive uropathy - prognostic factors - kidney recovery – ureteral stent – relief obstruction
ABSTRACTObstructive uropathy may lead to irreversible kidney damage. The insertion of ureteral stent is one of procedure to relief obstruction and prevent further kidney damaged, septicemia as well as urosepsis. A study to evaluate the predictive factor of urinary diversion in improving kidney function is needed. This study aimed to assess prognosis factors post-relief of obstruction in patients with uropathic obstruction focusing on kidney function recovery. This was a retrospective cohort study conducted at An-Nur Private Hospital in Yogyakarta Special Region, Indonesia using the medical record of patients with obstructive uropathy whom underwent DJ Stent insertion from a period of 2011 to 2015. The data of clinical assessment, laboratory and radiologic examination data were collected and analyzed using Chi square or student t test. A total 59 patients with obstructive uropathy aged around 50.9 years whom underwent DJ Stent were involved in this study. The results showed that release of obstruction with the installation of ureteral stents was significantly able to improve kidney function. The duration of obstruction, etiology, and hemoglobin (Hb) levels were associated with creatinine levels, whereas gender, urinary tract infection (UTI) and comorbid were not. In conclusion, prognosis factors of postrelief obstuction are duration of obstruction, etiology of obstruction and Hb levels before the release of obstruction
Universitas Gadjah Mada
2019-03-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/12351
10.19106/JMedScie/005004201803
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 4 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 4 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/12351/pdf
Copyright (c) 2019 R Danarto
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/12381
2019-10-31T02:47:45Z
bik:ART
Assessment of the normal anal position index (API) of Indonesian neonates
Suryana, Yudi
Makhmudi, Akhmad
anal position index - sex - gestational age – Indonesia - neonate
The existing anal position index (API) data appear to vary among the ethnic differences.Until now, however, the normal API has not been studied in Indonesia. This study aimedto determine the normal value of the API in Indonesian newborns This cross-sectionalstudy was conducted on 62 neonates (29 males and 33 females) without any malformationat Pediatric Surgery Division, Department of Surgery, Dr. Sardjito General Hospital,Yogyakarta during the period September to October 2012. The position of the anus wasnumerically defined by the API, which is the ratio of anus-fourchette distance in females and anus-scrotum distance in males to the distance between coccyx and fourchette/scrotum. To make correct measurements, transparent adhesive tape was used longitudinally on midperinum in a way that it covered the anus. Then fourchette/scrotum, anus center and the lower margin of coccyx were marked on it. Distances marked on each tape were then measured with the standard ruler. Relationship between API and other parameters were analyzed. The API values were 0.46±0.05 (95% CI: 0.44–0.48) for newborn males and 0.37±0.07 (95% CI: 0.35–0.39) for newborn females. The difference of API between males and females was significant (p = 0.000). API had no significant correlation with gestational age (p = 0.350) and birth weight (p = 0.650). Our data suggested the API, which is sex dependent, provides a reliable parameter for determining the position of the anus. API has no correlation with gestational age and birth weight. The determination of normal API in older infants is required to determine whether the API is affected by age.
Universitas Gadjah Mada
2019-01-28
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/12381
10.19106/JMedScie/005004201807
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 4 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 4 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/12381/pdf
Copyright (c) 2019 Yudi Suryana, Akhmad Makhmudi
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/12396
2019-08-08T06:04:08Z
bik:ART
The effects of duration of mesenteric artery ligation to the ratio of TNF-α/IL-10 in a rat model of acute mesenteric ischemia (AMI)
Handaya, Adeodatus Yuda
Budipramana, Vicky S.
acute mesenteric ischemia – mesenteric artery - TNF-α/IL-10 ratio – biomarker – early diagnosis
The mortality rate of acute mesenteric ischemia (AMI) is high due to the delay in diagnosis.Determination of potent biomarker for early AMI is the key in reducing the mortality. As aproinflammatory cytokine, the level of TNF-α might be affected during the ischemia andreperfusion, with the prediction duration of 60-120 min. High TNF-α level may stimulatethe upregulation of IL-10 as an inhibitor of TNF-α. This provides a new opportunity forearly diagnosis of AMI by measuring the ratio between those two cytokines. The purposeof this study was to investigate the effect of duration of the mesenteric artery to theratio of TNF-α/IL-10 in a AMI rat model. This was an experimental study using Wistarrat. We performed mesenteric artery in 28 male rats to produce an AMI model, withligation duration of 0, 30, 60, 90, 120, 150, and 180 minutes. At the end of ligation,blood samples were taken for measurement of TNF-α and IL-10 level using ELISA. For themicroscopic examination of tissue necrosis, intestinal organ samples were taken and madeinto paraffin blocks and stained using Haematoxylin-Eosin. TNF-α increased in minute 120compared to other treatment groups (p<0.05). IL-10 increased in minute 180 comparedto control group (p<0.05). Microscopic examination showed that the duration of ligationaffects the structure and morphology of intestinal mucosa characterized by discolorationof organs along with increasing the ligation duration. Ligation of the superior mesentericartery was found to be significantly increased the TNF-α level and to be compensated byincreasing IL-10. It is assumed that when the IL-10 level, that has protective effect as aninhibitor, higher than TNF-α level as a proinflammatory cytokine on duration 150 minutes,it means no more inflammatory or cells is dead. Therefore TNF-α/IL-10 ratio can be usedas a biomarker candidate of prognosic factor management of AMI.
Universitas Gadjah Mada
2017-12-27
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/12396
10.19106/JMedSci004904201702
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 49, No 4 (2017); 165-174
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 49, No 4 (2017); 165-174
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/12396/pdf_1
Copyright (c) 2017 Adeodatus Yuda Handaya, Vicky S. Budipramana
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/12577
2019-05-21T07:23:54Z
bik:ART
Prognostic factors affecting the mortality of 2nd and 3rd degree burn injuries at a tertiary care center in Indonesia
Dachlan, Ishandono
Anam, Khoirul
burn injuries – mortality – prognostic factors – tertiary care center - Indonesia
About two million people suffer from burn injuries in the United States eachyear, with 100,000 hospitalized in the burn unit. Around 1000 patients sufferfrom severe burn injuries, with each year average of 300 deaths. Improvementsin the understanding of the prognostic factors affecting burn injuries over thepast decades have led to advances in medical and surgical treatment. However,comprehensive data on the factors affecting burn injuries in Indonesia havenot been available, yet. The aim of the study was to investigate the prognosticfactors affecting the mortality of 2nd and 3rd burn injuries patients in Dr. SardjitoGeneral Hospital, Yogyakarta. This was a cross-sectional study conducted withinthe period of 2007-2011 using secondary data from the Department of MedicalRecords. Chi-square and logistic regression analysis were used to evaluate thecorrelation between the prognostic factors and the mortality. A p value < 0.05(95% confidence interval) was considered to be significant. A significantlycorrelation between age, burn injuries percentage, arrival time, inhalation trauma,hemoglobin level, albumin level, creatinine level, hematocrit level and the patient’smortality was observed in this study (p<0.05). However, the cause of burn injuriesand leukocyte count had no correlation with the patient’s mortality (p>0.05).Furthermore, patients with albumin level < 3.5 mg/dL, burn injuries percentage>50%, inhalation trauma and hospitalized in 24 hours after the incident were at22.98, 7.65, 3.0 and 4.59 times higher risk of mortality, respectively (p<0.05).In conclusion, albumin level, burn injury percentage, inhalation trauma and time ofarrival are prognostic factors affecting the mortality of the burn injuries patients.
Universitas Gadjah Mada
2018-10-05
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/12577
10.19106/JMedSci005002201809
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 2 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 2 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/12577/pdf
Copyright (c) 2018 Ishandono Dachlan, Khoirul Anam
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/12578
2019-11-14T07:32:11Z
bik:ART
Comparison of wound healing of skin incision on albino rat (Rattus norwegicus) by treatment of electrical stimulations
Herdiawan, Rina Puspasari
Rezano, Andri
Vitriana, .
Ruslina, Irma
Pitaloka, Pritha
Achadiyani, .
skin, wound healing, electrical stimulation, TENS, HVPC, LIDC
Wound interferes with the equilibrium of skin functions. It disrupts a barrier function of the skin as external barrier of the internal organ from physical, chemical and biological environment. The wound can be easily treated but neglected wound can lead to several complications. Accelerate wound healing will prevent complications and reduce aesthetic problem in anti-aging treatment. Previous studies showed that physical modulation as electrical stimulation could enhance wound healing processes. This study purposed to compare three different modes of electrical stimulation on wound healing such as transcutaneous electrical nerve stimulation (TENS), high voltage pulse current (HVPC) and low-intensity direct current (LIDC). This in vivo study used incisional skin biopsy of albino rat (Rattus norvegicus). Qualitative and quantitative parameters were analyzed to compare three different electrical stimulations on the wound healing response on the epidermis, dermis, inflammation, and angiogenesis phase. The highest histological score on the epidermis and dermis was found on LIDC whereas the highest histological score on the inflammation and angiogenesis phase was found on HVPC. This result of this study may provide useful information for selecting additional treatment for wound healing.
Universitas Gadjah Mada
2019-08-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/12578
10.19106/JMedSci005102201901
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 51, No 2 (2019); 98-105
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 51, No 2 (2019); 98-105
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/12578/pdf
Copyright (c) 2019 Rina Puspasari Herdiawan, Andri Rezano, . Vitriana, Irma Ruslina, Pritha Pitaloka, . Achadiyani
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/12663
2019-05-21T07:11:46Z
bik:ART
Chronic wound mitomycin-c-induced animal models
Ariawan, Andreas
Wicaksana, Aditya
Fauzi, Aditya Rifqi
Seswandhana*, Rosadi
chronic wound; wound profile; mitomycin-c
ABSTRACTChronic wound is a problem often encountered, especially in areas that do not have adequate health facilities. Some of the factors that caused the injury are mechanical, chemical, electrical, or heat. Chronic inflammation and bacterial infections are the two major factors that affect the process of wound chronicity. Mitomycin-C (MMC) is widely used as an intravenous, oral, and topical anti-cancer drug. MMC that is applied topically to a wound can cause cross-linking and decrease or stop the process of DNA transcription so that the wound will not develop the healing phase because MMC works as an inhibitor of fibroblast and proliferation that can inhibit wound healing. This study aimed to create animal models of chronic wound using topical MMC compared to those who given exposure of NaCl without topical MMC. This study used eight female Wistar rats aged 70 to 90 days, weighed between 300 to 350 grams. Wounds were made in the hip area with a diameter of approximately two centimeters. The subjects were divided into two groups. The first group, we compressed the wounds using sterile gauze moistened with MMC 0.5 mg/ml for 5 minutes and rinsed with a saline solution as much as 10 ml6. The second group was compressed using sterile gauze moistened with saline for 5 minutes. On day 3, 5, and 15 we observed the wound profile that was consist of diameter (as an indicator of wound healing), necrosis, and the consistency. The wounds that were compressed using MMC showed brownish-black, dry, thick chronic wounds and took longer to heal compared to those with NaCl. Application of MMC topically on the wound after day 15 showed an inhibition of wound healing process. The animal models showed a slower decrease of wound diameter, fewer scar tissue formation, and development of necrotic tissue.
Universitas Gadjah Mada
2018-10-05
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/12663
10.19106/JMedSci005002201803
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 2 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 2 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/12663/pdf
Copyright (c) 2018 Andreas Ariawan, Aditya Wicaksana, Aditya Rifqi Fauzi, Rosadi Seswandhana*
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/12781
2019-05-21T07:30:39Z
bik:ART
Effect of rich resistant starch snack on MCP- 1 promoter methylation and triglycerides levels in type 2 diabetes mellitus patients
Fadlilah, Synta Haqqul
Sunarti, .
Farmawati, Arta
Type 2 diabetes mellitus, resistant starch, DNA methylation, MCP-1, triglyceride
Type 2 diabetes mellitus (T2DM) is closely related to inflammation. One of inflammation marker in T2DM is monocyte chemoattractant protein-1 (MCP-1). Previous research suggests that MCP-1 promoter methylation correlated significantly with plasma triglycerides levels (TG) in T2DM. Some studies state that TG levels in T2DM can be controlled through consumption of diets containing resistant starch. This study aimed to investigate the effect of high resistant starch snack on MCP-1 promoter methylation and triglycerides levels in type 2 diabetes mellitus. This study was a cross-over trial. A total of 19 T2DM subjects have been selected with the criteria of fasting blood glucose levels (GDP)>126 mg/dL, aged 40-60 years, and duration of DM at least 1 year. Subject consume snack as much as 32 g/day with the resistant starch content are 4.25 g for 4 weeks. Data collection and blood retrieval was doing before and after the intervention. The analysis of MCP-1 promoter methylation was performed by methylated specific-PCR (MS-PCR) using DNA samples extracted from mononuclear cells, whereas TG levels analysis performed by the enzymatic colorimetric method (DiaSys Kit) using blood plasma. Statistic analysis was performed by Fisher Exact test for methylation data, paired t-test and unpaired t test for TG levels, and Spearman correlation test for the correlation between variabels (p<0.05). Methylation result showed that frequency of “methylated” (52.6%) and “unmethylated” (47.4%) status before and after intervention were same, so there was no significant difference (p>0.05). Plasma triglyceride levels after intervention decreased, but not significant statistically. The correlation between MCP-1 promoter methylation and plasma triglyceride levels was significant. This study concludes that the giving of rich resistant starch snacks intervention does not affect MCP-1 promoter methylation changes significantly. This intervention can lead to decrease plasma triglyceride levels subjects, but not statistically significant.
Universitas Gadjah Mada
2018-12-26
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/12781
10.19106/JMedSci005002201813
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 2 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 2 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/12781/pdf
Copyright (c) 2018 Synta Haqqul Fadlilah, . Sunarti, Arta Farmawati
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/12807
2019-08-08T06:04:08Z
bik:ART
The effect of hemofilter, preoperative and intraoperative methylprednisolone on complications after open heart surgery
Supomo, .
Aryandono, Teguh
Soesatyo, Marsetyawan
Sudiharto, Paulus
hemofilter – methylprednisolone – complication - open heart surgery – intraoperative
Complications after open heart surgery may threaten patient’s survival rate. Theintraoperative methylprednisolone administration alone shows controversial resultson open heart surgery complications. Similarly, the intraoperative and preoperativemethylprednisolone administration as well as the use of hemofilter in open heart surgeryis still controversial. This study aimed to evaluate the effect of hemofilter, preoperativeand intraoperative methylprednisolone administration on complications following openheart surgery. This was a Prospective Randomized Open-Blinded Evaluation (PROBE)experimental study. Ninety-five patients who had open heart surgery in Dr. SardjitoGeneral Hospital, Yogyakarta, and Integrated Cardiac Care of Dr. Cipto MangunkusumoGeneral Hospital, Jakarta within the period of December 2011 to May 2012 wereinvolved in this study. The patients were divided into two groups i.e. group A, 48 patientsreceived methylprednisolone 15mg/kg intraoperatively, methylprednisolone 5mg/kgpreoperatively, and hemofilter, while group B, 47 patients received methylprednisolone15mg/kg intraoperatively alone. From the total 95 patients, we found 26 (27.4%)patients experienced complications i.e. 19 in group B (40.4%) and 7 in group A(14.6%). The differences of the complications were statistically significant (p<0.05;OR=3.97; 95%CI=1.476-10.71). Complications risk decreased by 63.9% in the groupA compared to the group B with the hazard ratio of 3.2. In conclusion, the application ofhemofilter, preoperative and intraoperative methylprednisolone might decrease the risk ofcomplications after open heart surgery.
Universitas Gadjah Mada
2017-12-27
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/12807
10.19106/JMedSci004904201704
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 49, No 4 (2017); 183-190
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 49, No 4 (2017); 183-190
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/12807/pdf
Copyright (c) 2017 . Supomo, Teguh Aryandono, Marsetyawan Soesatyo, Paulus Sudiharto
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/12945
2019-08-08T05:40:18Z
bik:ART
Outcomes of surgical management of intracerebral hemorrhagic stroke at a tertiary care center in Yogyakarta, Indonesia
Hartanto, Rahmat Andi
Jauhardin, Teuku
intracerebral hemorrhagic stroke – surgical treatment – conservative management – outcomes
Hemorrhagic stroke remains a significant cause of morbidity and mortality worldwide. However, the role of surgical treatment for hemorrhagic stroke remains controversial. Previous studies had proposed surgery can prevent herniation, lowering intracranial pressure (ICP) and reducing mass effect and cellular toxicity. Outcome in previous studies are conflicting. Studies concerning outcomes of surgical management of hemorrhagic stroke in Indonesia are limited. This study aimed to compare the outcome of hemorrhagic stroke treatment between surgical evacuation and conservative management in Dr. Sardjito General Hospital, Yogyakarta, Indonesia. Retrospective cohort study was conducted. Eighty spontaneous intracerebral hemorrhagic (ICH) stroke patients involved in this study during January 2014 to August 2015. Of whom 36 (45%) male and 44 (55%) female. There were 45 and 35 patients underwent surgical treatment and conservative management, respectively. Surgical treatment group survival was 74%, whereas the survival in the conservative group was 26%. There was a statistically significant difference between surgery group and conservative group (p
Universitas Gadjah Mada
2018-10-05
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/12945
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 3 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 3 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/12945/pdf
Copyright (c) 2018 Rahmat Andi Hartanto, Teuku Jauhardin
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13595
2019-10-31T02:47:45Z
bik:ART
The effect of human saliva compared to Aloe vera on wound healing of 2nd degree burn injury in animal models
Putro, Budi Cahyono
Dachlan, Ishandono
human saliva – Aloe vera – natrium chloride – 2nd degree burn injury - model
Burn injury is one of the common causes of injury that has relatively high morbidity and mortality. Several studies using herbal and traditional medicine from different countries have been documented in burn injury management. Human saliva that contains antibacterial, antifungal, antiviral and analgesic components as well as growth factors can induce re-epithelialization process in 2nd degree burn injury. Whereas, Aloe vera that influence a physiological moist condition was proven can induce re-epithelialization process lead to faster wound healing. This study aimed to compare topical application of human saliva and A. vera on wound healing process of 2nd degree burn injury. This was an experimental study using post-test only control group design using 27 white rats (Rattus novergicus) of Sprague Dawley strain divided into 3 groups with 9 rats in each group. Group I were applied 1 mL of human saliva, Group II were applied A. vera and Group III as control were applied NaCl. Change in body weight and macroscopic clinical assessment were observed every day for 14 days, whereas histological examination was observed on day 14. The data were presented as mean ± standard error of the mean (SEM) and analyzed using one-way analysis of variance (Anova). The result showed that the wound healing process at each treatment showed different level. The human saliva application tended to show faster wound healing process of 2nd degree burn injury compare with A. vera or NaCl (p
Universitas Gadjah Mada
2019-10-31
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13595
10.19106/JMedScie/005004201801
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 4 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 4 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13595/pdf
https://jurnal.ugm.ac.id/bik/article/downloadSuppFile/13595/720
Copyright (c) 2019 Budi Cahyono Putro, Ishandono Dachlan
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13614
2017-10-30T01:34:13Z
bik:ART
Breast reconstruction
Kurnia, Ahmad
breast cancer, mastectomy, oncoplasty, reconstruction
Breast reconstruction is such a unique procedure that might potentially be adjustedin line with time and all advances in technology and instruments (endoscopy,silicone implants, alloderm grafting). In addition, it adapts in accordance to better,more sophisticated knowledge of breast anatomy (vascularization, perforator, andinnervation), breast subunits, and systemic changes. Surgical techniques and thecurrently available list of materials might be utilized to reconstruct the breast insuch a way to yield cosmetic satisfaction. Combination and modification of thosetechniques might be adjusted to the patients’ requests without crossing over thepaths of the disease being corrected (cancer, benign tumor, infection, or otherbreast anomalies).Breast reconstruction’s prerequisite is the absence of cancer approximately 1cm from the edge of incision, adjusted by shape and size of breasts, and sizeof cancer. Several techniques might be applied to conserve and reconstruct thebreasts when the disease has been diagnosed. Oncoplasty applies all techniquesavailable to reaffirm the principles of oncology, by increasing the distance fromthe edges of cancer and proceeding with reconstruction by reduction/mastopexy(volume displacement), or adjacent/distant flaps (volume replacement). Thedisadvantage of BCS/BCT is the short distance from the edge of incision to thetumor due to the risk of post-operative breast deformity, especially when surgeryis followed by adjuvant radiation.
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13614
10.19106/JMedScieSup004804201601
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13614/pdf
https://jurnal.ugm.ac.id/bik/article/downloadSuppFile/13614/724
Copyright (c) 2017 Ahmad Kurnia
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13616
2017-10-30T07:16:24Z
bik:ART
The role of Clinical Geneticists in Hereditary Cancer Management and Research
Heijboer, Hanne Meijers
clinical genetics, germline, hereditary cancers, cancer predisposing genes
AbstractHereditary cancer refers to cancers caused by germline mutations in cancer predisposing genes. These mutations confer a significantly increased risk of cancer, are rare, and are in the majority of cases autosomal dominantly inherited. Since the eighties of last century more than 115 cancer predisposing genes have been identified. In many Western countries genetic testing of patients and families with clustering of cancers started early, and was often performed by clinical geneticists (MDs performed the counselling and pedigree analyses) and by molecular biologists (in laboratories within departments of clinical genetics). It turned out to be a long path to fully realize the promise of cancer predisposing genes. The clinical utility of many cancer genetic tests and subsequent risk reducing interventions has not yet been validated and pitfalls in e.g. misinterpretation of genetic variants showed up. However, without doubt genetic testing for mutations will eventually turn out as a strong tool to save lives from early cancer death and will become part of standard cancer care throughout the developed world. Apart from primary surgical prevention, major progress is to be expected in earlier diagnoses, tailored therapies, and possibly chemoprevention. Ideally researchers, clinical geneticists, molecular biologists, surgeons, oncologists, gynaecologists and other professionals will work together to reach this goal.
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13616
10.19106/JMedScieSup0048042016012
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13616/pdf
https://jurnal.ugm.ac.id/bik/article/downloadSuppFile/13616/725
Copyright (c) 2017 Hanne Meijers Heijboer
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13617
2017-10-30T07:11:33Z
bik:ART
HBOC in Europe
Heijboer, Hanne Meijers
BRCA1, BRCA2, HBOC, Europe, Founder mutations, linkage
AbstractEurope has contributed to the majority of high-impact papers on genes and risk alleles in breast cancer and ovarian cancer susceptibility. Consortia like the Breast Cancer Linkage Consortium, Breast Cancer Association Consortium, and the Consortium of Investigators on Modifying genes in BRCA1/2, started from the nineties of the last century on. Many highly motivated participants throughout Europe, the US, Australia and elsewhere contributed to the formation of huge datasets. Instrumental of the success of the consortia was also the leadership and knowledge of Dough Easton, UK, on the statistics of cancer genetics. The consortia papers have produced the data on which national guidelines in HBOC were formulated in West-Europe, the US and worldwide. Genetic testing for HBOC is in most Western-European countries accepted and funded. Two decades after the identification of BRCA1 and BRCA2, attitudes towards genetic testing for HBOC are changing and becoming more ‘common’ practice. This offers opportunities to organize cancer genetic care more efficiently and at lower costs while informed decision making and consent of the patients remain in place.
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13617
10.19106/JMedScieSup0048042016011
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13617/pdf
https://jurnal.ugm.ac.id/bik/article/downloadSuppFile/13617/726
Copyright (c) 2017 Hanne Meijers Heijboer
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13620
2017-11-01T02:20:13Z
bik:ART
Cytogenetic Analysis for Research and Services
Faradz, Sultana MH
cytogenetics, chromosome, syndrome, DSD, hematologic malignancy
AbstractThat the correct chromosome number in man is 46 was first recognized by Tjio and Levan in 1956. Perhaps few Indonesians know that Tjio was an Indonesian scientist studying in Sweden and then living in the US. Cytogenetic analyses are commonly performed to determine both structural and numerical chromosome aberration, whilst changes in chromosomes can lead to birth defects, syndromes, or even cancer. Several chromosomal aneuploidy syndromes were identified after the establishment of various chromosome banding techniques in late 1960’s. Specific cell culture media was found to express fragile site in the beginning of 1970’s and since then, inherited Fragile X Mental Retardation syndrome could be diagnosed. However, some female permutation cases have been often misdiagnosed. Further molecular analysis has resolved this problem by revealing more CGG repeats in the promoter region FMR1 gene, which is related to the expression of fragile site and the severity of the diseases.In Disorder of Sex Development (DSD), early gender assignment and reconstruction surgery has been challenged because of the dilemma of gender identity development in later life. Cytogenetic analysis for the first-line gender assignment is important in newborn with DSD. Proper diagnosis with hormonal and mutation analysis should be elucidated to avoid medical, psychological, and social aspect in adult life. The most frequent genetic cases in our clinical experiences have been Androgen Insensitivity Syndrome and Congenital Adrenal Hyperplasia. Female Complete Androgen Insensitivity Syndrome (CAIS) with main symptom primary amenorrhea without cytogenetic analysis has often been diagnosed as inguinal hernia because of testicle location and size.Diagnosis and treatment of several leukemias and lymphomas, as well as some solid tumors, depend on cytogenetic analyses to demonstrate consistent, specific chromosomal aberrations. Chromosome analysis in hematologic malignancy is indicated to support diagnosis, select therapy regimen, and elaborate prognosis. Specific chromosome translocations have been identified for hematologic malignancy. The breakpoints of several of these translocations have been cloned. Several loci of oncogene have been identified and sequenced. Molecular genetic analysis will replace cytogenetic analysis and shift the requirement for studying metaphase cells. Therefore, chromosome analysis in genetic disease and cancer should be attained with advanced molecular techniques, such as Fluorescence In Situ Hybridization (FISH) and microarray CGH analysis. Cytogenetic analysis is still useful and applicable in genetic disease diagnosis, sexual assignment, and hematologic malignancy in the laboratory with minimal equipments. Molecular analysis as a part of health care services in Indonesia has been limited in research centers in university setting; therefore, a comprehensive diagnosis with genetic analysis has often been improbable.
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13620
10.19106/JMedScieSup004804201631
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13620/pdf
https://jurnal.ugm.ac.id/bik/article/downloadSuppFile/13620/727
Copyright (c) 2017 Sultana MH Faradz
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13627
2017-10-30T06:21:49Z
bik:ART
Molecular Genetics, Genetic Testing, Novel Genome Sequencing Technologies
Pals, Gerard
molecular genetics, diagnostics, next generation sequencing
AbstractWith the advance of genomic technologies, we are now able to detect genetic variations in patients with high accuracy, whole genome scale and relatively cost-effective. This offers an opportunity for altering medical practice fundamentally as well as insurance policy. Although clinicians, scientists, and health policy makers still have to deal how to interpret and handle the results that sometimes come with ambiguity and uncertainty, recent advances especially in the western world have integrated genetic tests and molecular genetic analysis for clinical management of patients. In this session, we will discuss and review the range of methods currently used in clinical setting as well as potential emerging methods in clinical molecular genetic diagnostics. Advantages and disadvantages of each methods will be carefully discussed especially application in regions of the world that have more limited access for molecular genetic tests including next generation sequencing. Outline of implementation challenges for molecular genetic tests both in term of health economics and clinical management will also be discussed.
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13627
10.19106/JMedScieSup004804201609
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13627/pdf
Copyright (c) 2017 Gerard Pals
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13628
2017-11-01T02:29:06Z
bik:ART
BRCA1 and BRCA2 Germline Mutations in Asian and European Populations
Hamann, Ute
BRCA1, BRCA2, founder mutations, hereditary breast cancer
Women who carry a pathogenic mutation in the breast cancer susceptibility genes BRCA1 or BRCA2 (BRCA) have markedly increased risks of developing breast and ovarian cancers during their lifetime. It has been estimated that their breast and ovarian cancer risks are in the range of 46-87% and 15-68%, respectively. Therefore it is of utmost clinical importance to identify BRCA mutation carriers in order to target unaffected women for prevention and/or close surveillance and to help affected women choose the best chemotherapy regimen.Genetic testing for BRCA germline mutations is expanding in clinical oncology centers worldwide. Given the high costs of complete BRCA gene screens, a lot of effort has been expended on deciding upon whom to test. Relevant issues involved in decision making include the prior probability of a woman having a BRCA mutation, which is a function of her age and her disease status, her ethnic group, and her family history of breast or ovarian cancer.The frequency and spectrum of mutations in these genes show considerable variation by ethnic groups and by geographic regions. Most studies have been conducted in European and North American populations, while studies in Asian, Hispanic, and African populations are fewer. In most populations, many BRCA mutations were identified, which were distributed all over the genes. However, in some populations, a relatively small number of specific BRCA mutations are recurrent and account for the majority of all mutations in that population. Many of the recurrent mutations are founder mutations, which were derived from a common ancestor. Founder mutations are present in Ashkenazi Jewish, European, and Islander (Faroe, Easter, and Pitcairn) populations. Such mutations have also been identified in patients from several Asian, South American, and African countries. Population-specific genetic risk assessment and genetic mutation screening have been facilitated at low costs. Given that mutations in the BRCA genes are distributed in populations throughout the world, it is important that the benefits of genetic testing and of targeted therapies be made available not only to women from developed countries in Europe and North America, but also to those from less developed countries in Asia, Africa and South America.
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13628
10.19106/JMedScieSup004804201635
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13628/pdf
Copyright (c) 2017 Ute Hamann
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13629
2017-11-01T01:58:06Z
bik:ART
Bacterionomics and vironomics in carcinogenesis
Sudarmono, Pratiwi
oncogenic bacterial, viral, oncogenesis, suppressor, oncogene
Virus and bacteria are microbes which are very common cause human infection. Most of the bacterial infection can be eradicated by antibiotics and infection symptoms disappear. But for virus infection, once infected, the virus will persistently stay in the host, even undergo not only a lytic cycle but also integrated into host genome. Nowadays, at least 6 virus type are consistently related to human cancer, such as EBV,HPV,HTLV,HBV,HCV,HKSV, and the new one Merkel Virus (MCV). Although not every infected people will get cancer, but around 20% of the whole cancer in human are caused by viral oncogene.Class one oncogenic bacterial is Helicobacter pylori. Infection with this bacteria can cause persistent gastro duodenal inflammation which cause some alteration in gastric cell growth into transformation. Expression of Cag gene and Vac gene and some expression of OMP protein usually link to gastric cancer.Molecular mechanisms of carcinogenesis for every virus which cause infection is a very complex , which include several processes caused by cell transformation. Besides, other host and environmental factors are also play a significant role in cancer development. Some scientist put a Hallmark analysis as a model to quickly summarize what pathobiology process will happen and what gene or protein caused the process. The Hallmark analysis comprise of several process which may happen simultaneously because some of the Hallmark is caused by the same protein. The Hallmark consists of various virus strategies in oncogenesis such as promoting angiogenesis, avoiding immune destruction, genome instability and mutation, deregulating cellular energetic, resisting cell death, sustaining proliferative signaling, evading growth suppressors, enabling cellular immortality, promoting inflammation and activation metastasis. For example, infection by HPV, will cause low grade dysplasia which can continue to invasive cervical cancer. After host cell transformation, in the long control region genes, E6 and E7 protein will cause several strategies in oncogenesis including resisting cell death and evading growth suppressors. HBV infection will end without any serious liver damage, but after cell transformation, almost all Hallmark strategies of viral oncogenesis are happening step by step in line with the severity of liver cell damage.As the onset of cancer development after infection can last years, there are an opportunity to design either vaccine or genetic therapy to minimalize further risk of cancer in human
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13629
10.19106/JMedScieSup004804201626
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13629/pdf
Copyright (c) 2017 Pratiwi Sudarmono
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13630
2017-11-01T02:01:04Z
bik:ART
Pharmacogenomics and Pharmacogenetics: Some Cases in Oncology
Yuliwulandari, Rika
pharmacogenomics, pharmacogenetics, germline, GWAS, next-generation sequencing
ABSTRACTPharmacogenomics and pharmacogenetics plays an important role in understanding how genetic variants influence drug efficacy and toxicity. In the case of cancer, both efficacy and toxicity of therapeutic agents determine the improvement of survival and quality of life in cancer patients. Effective treatment of Cancer, one of the most deadly diseases in the world, is importance for extending patient survival. Genetic variation influences the response of an individual to drug treatments that impact the efficacy and toxicity of the drugs. Understanding individual genetic variation is potential to make therapy safer and more effective by determining more appropriate drug selection and drug dosage for each individual patient. In the context of cancer, tumors may have specific disease-defining mutations, but a patient’s germline genetic variation will also affect drug response. Advance research technologies approach such as GWAS and next-generation sequencing technologies, statistical genetics analysis methods and clinical trial design have shown promise for discovery of genetic variants associated with drug response in cancer. Understanding the molecular characteristics of both the tumor and the patient, and establishing their relation with drug outcomes will be critical for the identification of predictive biomarkers and to provide the basis for individualized treatments. Since cancer is also frequent in Indonesia, pharmacogenomics study in oncology and its implementation in the clinical practice is important. Broad collaboration at national and international level could foster the application of personalized medicine in oncology.
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13630
10.19106/JMedScieSup004804201627
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13630/pdf
Copyright (c) 2017 Rika Yuliwulandari
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13632
2017-11-01T02:18:53Z
bik:ART
Long Non-Coding RNA (lncRNA) and MicroRNA ( miRNA) in Cancer Management
Haryana, Sofia Mubarika
miRNA, ncRNA, LncRNA, regulation, cancer management
AbstractThe discovery of microRNA, a small non coding RNA, has shed light to the dark matters (98%) of the genome. This finding resulted in a Nobel Prize awarded to Fire and Mello in 2006. miRNA a small non coding RNA which played a very important role in regulating protein expression through 3”UTR or other binding places to mRNA target. miRNA have been considered as negative regulators of protein coding gene expression that may impact in cell differentiation, proliferation, survival and all fundamental cellular processes, also implicated in carcinogenesis. miRNA can be grouped into tumor suppressor miRNA (miRSuppressor) and oncogenic miRNA (OncomiR). miRSuppressor regulates protein expression through targeting oncogenic mRNA, meanwhile OncomiR target mRNA Tumor Suppressor. Evidence indicates that deregulation in genetic and epigenetic may cause overexpression of oncomiR and loss of expression of Tumor Suppressor miR. In addition to that, in recent years, evidences showed that cell-to-cell communication conducted via exosome, which is released from every cell in physiological and pathological conditions andconsidered as fingerprints of cell and its status. This is a paramount biomarker discovery in cancer. In subsequent years, a lot of research further performed for the development of new cancer therapy. Our team GenomiR present our preliminary data on several miRNA in cancers aimed to develop minimal invasive biomarkers in cancer. Recently, the long non coding (lnc) RNA, another class of non-coding RNA have also attracted interest from many scientists in the world. lncRNA have emerged as an essential regulator in almost all aspect of biology included carcinogenesis. lncRNA considered as emerging key player in non-coding world.nCRNA (miRNA and lncRNA) in the context of cancer management will be discussed in this presentation
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13632
10.19106/JMedScieSup004804201630
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13632/pdf
Copyright (c) 2017 Sofia Mubarika Haryana
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13633
2017-10-30T08:27:03Z
bik:ART
Proposed Organization of Family Cancer Clinics in Indonesia
Setiaji, Kunta
familial cancer, hereditary cancer, genetic counseling, family cancer clinics
AbstractAround 10-15% of breast cancers are associated hereditary and/or familial predisposition. By definition familial breast occurs in two or more first degree relatives within a nuclear pedigree (first or second degree relatives). Hereditary and familial cancer displays different characteristics in the pathological features, clinical course, response to treatment, and outcomes. Therefore, specific consultation and treatment need to be addressed to patients with hereditary or familial predisposition for example the need for rigorous surveillance and preventive treatment including options for preventive surgery. Cancer clinical genetic service is not yet formally available in daily clinical practice in Indonesia. Surgeons usually become the first medical specialist to see cancer patients with familial predisposition, therefore they have to elaborate clinical cancer genetic service under Family Cancer Clinic (FCC). Clinical genetic service within FCC consists of several step-wise tasks including assessment of personal and family history of cancer, personalized cancer risk assessment, review of medical and family history, individual cancer screening and surveillance recommendations, genetic testing if necessary, discussion of benefits and limitations of genetic test, cancer risk reduction options and preventive strategies, and opportunity to participate in research as well as clinical trial. Nation-wide network for FCC is of importance to share knowledge and skill to perform cancer genetic service. Ability to perform genetic test including the interpretation in Indonesia has also been required.
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13633
10.19106/JMedScieSup0048042016016
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13633/pdf
Copyright (c) 2017 Kunta Setiaji
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13634
2017-11-01T02:16:40Z
bik:ART
BRCA1 and BRCA2: Lack of Certainty and Its Clinical Implications
Haryono, Samuel J
BRCA1, BRCA2, VUS, interpretation, clinical management
AbstractIn this time of amounting cancer incidence and mortality, genetic testing may be greatly valuable in deciding on appropriate clinical management. It is unfortunate that the test is not a practical interrogative problem. When positive, the patients can settle down with what to do next, whereas when negative, their family members may breathe out in relief from unnecessary intensive surveillance and prophylaxis, and then there is VUS (Variants of Uncertain Significance). How should a health provider deliver the elaboration and implication of VUS to a patient who had expended a lump sum of and had expected a level of certainty? Variants of UncertainSignificance have nowadays become a challenging spoiler in the setting of clinical management of cancer. How would one inform someone else on anything uninformative? BRCA1 and BRCA2 are two genes with high penetrance in breast cancer. Since their functions as tumor suppressor genes and DNA repair regulators, their mutation effects are only visible when there is Loss of Heterozygosity. Mutation of BRCA1/2 gene can be demonstrated within the tissue specimen and blood sample DNA; that would mean it has occupied all tissues and is inheritable. In the case of BRCA1 and BRCA2 mutations, 10-20% of all genetic test results will read VUS, In one previous study of sixteen Indonesian patients, 13 (81,25%) patients had VUS. There were variants that had not been found in other population. Trans-academically speaking, the reclassification of VUS in BRCA1/2 gene is not merely a challenge to clarify its clinical impacts, but also an obligation to accomplish our community contribution to science.There can be a set of factors to suggest that a VUS may be a deleterious mutation:Co-segregation: when the variant comes with multiple and multigenerational incidence of cancer, it is possible.Epidemiology: when a case control study demonstrates prevalence discrepancy, it is possible.Co-occurrence with deleterious mutation: when the variant is shared within the same gene in other individuals, it is possible.Evolutionary data: when the sequence is carried across species, it is possible.Amino acid substitution: when the substitute is structurally similar, it is impossible.Loss of heterozygosity: when there is loss of a wild type allele in tumor specimen, it is possible.Functional analysis: should an in vitro assay demonstrate a loss of protein function, it is probable.The ultimate solution is not yet available. However, there is clinical significance for families with VUSs, only if that can confidently be classified as the presence or absence of the associated disease. That condition may be achieved by increasing the availability of genetic testing so that there can be a larger, open-access repertoire of VUSs.
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13634
10.19106/JMedScieSup004804201629
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13634/pdf
Copyright (c) 2017 Samuel J Haryono
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13637
2017-10-30T06:13:38Z
bik:ART
Management of Hereditary Breast and Ovarian Cancer. The Asian Experience
Kwong, Ava
BRCA1/BRCA2, germline, HBOC, Asia BRCA Consortium, NGS
BRCA1/BRCA2 mutations are the most common high penetrant genes associated with an increased lifetime risk for hereditary breast and ovarian cancer (HBOC). Although genetic testing is standard of care in Western developed countries, there are still variations in availability of genetic testing and risk assessment for HBOC in Asia. Depending on the countries, there are variations in the clinical strategies and cancer management. The Asian BRCA Consortium has grouped together 14 Asian countries and reviewed genetic counselling/testing uptake rates and clinical management options in these countries. Moreover economic factors, healthcare and legal frameworks, and cultural issues affecting the genetic service availability in Asia were discussed. Mutation spectrum, and VUS rates and the increase use of NGS gene panel testing poses more decisional issues in the clinical management of Hereditary Breast cancer in Asia. These will be discussed.Keywords: BRCA1/BRCA2, germline, HBOC, Asia BRCA Consortium, NGS
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13637
10.19106/JMedScieSup004804201608
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13637/pdf
Copyright (c) 2017 Ava Kwong
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13638
2017-10-30T06:08:26Z
bik:ART
Management of hereditary breast cancer: Surgeon's perspective
Kwong, Ava
mutations, BRCA1. BRCA2. TP53, PTEN, NGS, multidisciplinary setting
Mutations due to hereditary related genes such as BRCA1, BRCA2, TP53 and PTEN confer greater risk of developing breast cancer and for BRCA mutations, also ovarian cancer. The risk assessment based on genetic testing allows options of high risk surveillance, prevention and may now also guide use of specific therapies for treatment such as targeted therapies and use of platinum base chemotherapy. The choice of management, once an individual has been found to carry the BRCA mutation may also vary. Moreover the availability of genetic testing, method of testing such as the transition into the use of Next Generation Sequencing techniques has also increased options of clinicians to the choice of testing.Breast Surgeons are most likely to be the first person who encounters the first presentation of a breast cancer patient. It is important for breast surgeons to be actively involved in the referrals of patients for genetic testing and subsequently planning of the management of such high risk individuals in a multidisciplinary setting. Basic principles of genetic testing and choice of management will be discussed in reference to the surgeon’s perspectives. Keywords: mutations, BRCA1. BRCA2. TP53, PTEN, NGS, multidisciplinary setting
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13638
10.19106/JMedScieSup004804201606
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13638/pdf
Copyright (c) 2017 Ava Kwong
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13640
2017-10-30T06:11:46Z
bik:ART
Oncoplastic breast surgery in Asia
Kwong, Ava
breast cancer; oncoplastic breast surgery; Asia
Oncoplastic breast conservation surgery has given a new light to the options of performing breast conserving surgery by combining oncological principles of breast cancer surgery with plastic surgery techniques. The ultimate oncoplastic achievement would be the conversion of what normally would be considered as an oncologic and/or cosmetic failure when using standard techniques of breast conserving surgery into both oncological and cosmetic success and to avoid mastectomy where possible.Most Techniques are fairly well established. However surgical techniques may also need to be adjusted based on the cohorts that the treatment is offered to. For example, for larger breasts, the technique is more forgiving. However for smaller breast, there is still a limitation of the cosmetic outcome when a high percentage of breast volume has been excised no matter how good the technique or the surgeon is.Various aspects of oncoplastic breast surgery technique will be discussed and its applicability to Asian Cohorts.
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13640
10.19106/JMedScieSup004804201607
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13640/pdf
Copyright (c) 2017 Ava Kwong
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13642
2017-11-01T02:27:27Z
bik:ART
Implementation of Medical Genetics in Medical Education Curriculum
Aryandono, Teguh
Primary care physicians should be aware that genomics has arrived at the doorstep of their practice. Genetic knowledge, skills, and attitudes are important to primary care physicians providing support and management to patients and families with (higher risks of) genetic conditions. At least one in ten patients seen in primary care has a disorder with a genetic component, including hereditary cancer.Primary care physicians must be able to advise patients on genetic and genomic manifestations of the associated diseases and disorders, to outline a primary pedigree to deliver the necessary information: the disease which the patients are at risk of, and to decide whether to refer the patients to genetic services.There is more and more evidence to the importance of clinical emphasis in the genetics classes, probably proceeding to a third-year refresher program in the longitudinal curriculum. An enhanced educational experience to improve genetics and genomics knowledge should be better structured in the imminent future.Keywords: primary care physicians, hereditary cancer, genetic curriculum, genetic services
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13642
10.19106/JMedScieSup004804201634
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13642/pdf
Copyright (c) 2017 Teguh Aryandono
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13644
2017-10-30T07:59:35Z
bik:ART
CLINICOPATHOLOGICAL FEATURES OF YOUNG AGE BREAST CANCER PATIENTS IN BALI
Sudarsa, I Wayan
young age breast cancer, clinicopathological features.
AbstractBreast cancer is, in general, a heterogeneous disease with diverse biological characteristics, histopathological types, subtypes and clinical behavior. Breast cancer in young age, although rare, is usually a unique and more aggressive cancer associated with poorer prognosis. The combination of young age and advanced stages of breast cancer would make this particular breast cancer more difficult to manage. Therefore, individually tailored therapies have been the subject of many studies for these patients. The purpose of this study was to determine clinicopathological features of young age breast cancer patients in Bali from 2014-2016. This was a descriptive study of young age (≤40 years) breast cancer patients in Bali from January 2014 until June 2016. The samples were taken from the Perhimpunan Ahli Bedah Onkologi Indonesia Cancer registry in Sanglah General Hospital Bali. There were 90 samples gathered from the cancer registry and 56 samples (62,2%) had complete clinicopathological records. Clinicopathological features included in this study were age group, histopathological type, primary tumor size, regional lymph node involvement, presence of distant metastasis, stage regrouping, tumor grade, hormonal receptor status, Her2 overexpression status, and breast cancer subtype. There were 56 young age breast cancer patients, with a mean age of 33,86±4,193 years old, the youngest patient was 22 years old, and the majority of the samples were in the 31-35 years age group (26 samples, 46,4%). Only 2 samples (3,6%) had special type carcinoma, both of them were invasive lobular carcinoma, the rest of the samples were invasive carcinoma of no special type. The 2 samples with invasive lobular carcinoma were in 31-35 years age group, had stage II (T2N0M0) and III (T4N1M0) breast cancer, both with grade II tumor and Luminal A subtype. The majority of primary tumor size was T4 (30 samples, 53,6%), nodal status was N1 (29 samples, 51,8%), and only 10 samples (17,9%) had distant metastasis. The majority of the stage regrouping was in stage III (35 samples, 62,5%) and had grade III tumor (33 samples, 58,9%). There were 10 samples (17,9%) with Luminal A subtype, 19 samples (33,9%) with Luminal B subtype, 16 samples (28,6%) with Her2 type subtype, and 11 samples (19,6%) with triple negative breast cancer subtype. We concluded that the majority of young age breast cancer patients in Bali were invasive carcinoma of no special type in advanced stage, with high grade tumor, and within Luminal B subtype.
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13644
10.19106/JMedScieSup0048042016014
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13644/pdf
Copyright (c) 2017 I Wayan Sudarsa
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13646
2017-11-01T01:21:10Z
bik:ART
One Step Nucleic Acid Amplification (OSNA) Study in Indonesia
Haryono, Samuel J
Sari, Lenny
Sugiharto, Sony
Datasena, I Gusti Bagus
Mulyarahardja, Raymond
Rudianto, .
OSNA, Sentinel Nodes, Breast Cancer, CK19
ABSTRACTSentinel lymph node (SLN) is defined as the first of a few selected lymphatic nodes, into which lymphatic fluid from a primary tumor drains. Streamlined processing of sentinel lymph nodes (SLN) for detection of lymph node metastasis involves the able command over methodical blocks of SLN identification, surgical removal of SLN and SLN analysis. One Step Nucleic Acid Amplification (OSNA) method, which relies on CK19 mRNA expression to detect intraoperatively lymph node metastases in breast cancer cases, emerged as a plausible alternative to the current gold standard that uses histopathological node analysis. Sixty selected axillary sentinel lymph nodes from thirty breast cancer patients. Sentinel lymph nodes were directly bi-halved after collection using customized lymph node cutting device (Sysmex), or scalpel. The first halves were subjected to histopathological examination and were stored in specimen containers containing fresh formaldehyde prior to processing. The adjacent halves were weighed to comply with the required mass by OSNA detection in the range of 50 – 600 mg and wrapped in clean foils for storage in -80°C prior to OSNA analysis. 60 SLNs were same diagnosis using both methods. 25 SLNs were negative and 25 SLNs were positive using both methods. 3 SLNs were positive on OSNA but negative on histology. Other 7 SLNs were negative on OSNA but positive on histology, and these 1 nodes contained only micrometastasis lesion. These results suggest that OSNA is a useful for detecting SLNs metastasis, but a copy number of CK19 might be an indepedent factor from prediction and prognosis of breast cancer.
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13646
10.19106/JMedScieSup004804201625
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13646/pdf
Copyright (c) 2017 Samuel J Haryono, Lenny Sari, Sony Sugiharto, I Gusti Bagus Datasena, Raymond Mulyarahardja, . Rudianto
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13647
2017-10-31T06:57:51Z
bik:ART
A Comprehensive Exploration of Java Man: Bio-Cultural Evolution from Homo erectus to Homo sapiens
Haryono, Samuel J
Utami, Sri
Rahayuningsih, Restu Ambar
ABSTRACTAn overlap of time period between Homo erectus and Homo sapiens has not been confirmed. In the history of man, there have been two missing links: one between man and ape, and one between progressive Homo erectus and archaic Homo sapiens. Specimen dating on Java Man has been discrepant among research groups, and the use of molecular biology in ancient specimens has been a novelty. This study intends to use fossilised specimens, to harvest DNA to be sequenced for ribosomal DNA analysis for comparative phylogeny among ancient and modern man and other hominids. Dental calculus will be analysed to identify starch, carbohydrate, and protein to illustrate paleo dietary pattern. Soil samples will be examined for pollen and phytoliths to elaborate on ancient ecosystem. Blood samples will be procured from indigenous people along the riverflow region of Bengawan Solo to analyse modern human DNA. We hope that we may reconstruct the evolution pathway, construct the phylogenetic tree between ancient and modern hominids, and discover the uniqueness of Homo sapiens sapiens.Keywords: Java Man, Ribosomal DNA, Hominid Phylogenetic,
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13647
10.19106/JMedScieSup0048042016024
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13647/pdf
Copyright (c) 2017 Samuel J Haryono, Sri Utami, Restu Ambar Rahayuningsih
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13648
2017-10-31T06:50:19Z
bik:ART
High Resolution Melting (HRM) Analysis for Genetic Changes in BRCA1/2 gene
Haryono, Samuel J
Datasena, I Gusti Bagus
Hariadi, Ariananda
Mulyarahardj, Raymond
Conventional mutation analysis requires a separation step and include single-strand conformational polymorphism (SSCP) analysis, denaturing gradient gel electrophoresis, heteroduplex analysis, denaturing HPLC, and temperature gradient capillary electrophoresis These methods require separation of PCR products on a gel or other matrix, often take hours to perform, and increase the risk of contamination in future reactions because PCR products are exposed to the environment. High Resolution Melting (HRM) can simplify the mutation scanning analysis in BRCA 1/2 gene. DNA from affected patients and family members were amplified with Real-Time PCR reaction and followed by Sanger Sequencing to reconfirm the mutation status if mutation obtained by HRM Method. HRM Method was able to show distinction in differential curves of mutated BRCA 2 gene c.4600T>C, with codon modification of CAT>TAT, when compared to wildtype. To determine point mutation in a sample, this method requires two groups of experimental standards and standard curves. The first standard produced by using samples without mutation (wildtype/negative control) and the second standard produced by using samples with mutation (positive control), that have been confirmed with Sanger Sequencing. The sequencing analysis of the affected patient and the family members showed that a mutation occurred (BRCA2 c.4600T>C) and was segregated in the family history. This mutation caused amino acid alteration in BRCA2 protein (p.H1458Y). HRM Method is an excellent tool to analyze genetic modification of BRCA1/2 genes, especially to investigate co-segregation of mutated genes among family members of affected patient. This method can provide more sensitive results to determine mutation in patient, before using Sanger Sequencing analysis.Keyword: BRCA, HRM, Gene Mutation
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13648
10.19106/JMedScieSup004804201623
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13648/pdf
Copyright (c) 2017 Samuel J Haryono, I Gusti Bagus Datasena, Ariananda Hariadi, Raymond Mulyarahardj
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13649
2017-10-31T06:32:10Z
bik:ART
Variation of the breast cancer susceptibility marker, rs4245739, is associated with differential miRNA binding and MDM4 expression
Anwar, Sumadi Lukman
Carlos-Roman, Angel
Wulaningsih, Wahyu
Watkins, Johnathan
A polymorphism, rs4245739, has been associated with susceptibility of several cancers including ER-negative breast cancer. Rs4245739 is located at the 3’UTR of MDM4 gene, an oncogene that negatively regulates p53. The polymorphism has been associated with binding changes of miR-191. We studied, the influence of SNP rs4245739 to the binding of microRNAs, expression of microRNAs and MDM4. Using FindTar software, we detected potential microRNAs affected by the SNP-flanking sequence. We then used RNA sequencing data from ER-negative breast cancer to compare expression of miR-184, miR-191, miR-193a, miR-378, and MDM4 in different genotypes. Comparison of ER-negative patients with and without expression of miR-191 as well as profile microRNAs (miR-184, miR-191, miR-193a and miR-378 altogether) can differentiate expression of MDM4between different alleles. In addition, the number of lymphatic nodes affected in the individuals was also found to be significantly reduced in the risk group obtained by the miRNA profile method. We show our methods especially miRNA profile approach, are able to obtain new molecular and clinical features related to the rs4245739 SNP, a variant located in the 3’UTR of MDM4 gene and known to appear in different types of cancer. Keywords: ER negative breast cancer, rs4245739, microRNA, MDM4, p53
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13649
10.19106/JMedScieSup004804201621
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13649/pdf
Copyright (c) 2017 Sumadi Lukman Anwar, Angel Carlos-Roman, Wahyu Wulaningsih, Johnathan Watkins
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13650
2017-10-31T06:16:36Z
bik:ART
The Expression of microRNA-155 and mRNA Hypoxia Inducible Factor Alpha (HIF1A) in the early and advanced stages of ovarian cancer patients blood plasma
Chasanah, S.N.
Fitriawan, A.S.
Pukan, F.K.
Kartika, A.I.
Oktriani, R,
Trirahmanto, A.
Pradjatmo, H.
Aryandono, T.
Haryana, S.M.
Ovarian cancer has the problem turned out to be the greatest and almost half the death rate of mortality throughout gynecological malignancy. Ovarian cancer is found in the female reproductive organs and the second most common cancer after cervical cancer. The process of carcinogenesis of ovarian cancer occurs at the molecular level, regulated by microRNA. At in silico research that has been done, it is known that microRNA-155 (miR-155) targeted mRNA HIF1A which is the regulator genes in hypoxia conditions. HIF1A involved in various cancer hallmarks, where some of them have roles in Warburg effect and also as genetical transcription factors in angiogenesis. Regulation of miR-155 and mRNA HIF1A believed to be involved in the process of ovarian cancer progression and thus potentially as minimally invasive biomarker for prognosis. The aim of this study is to determine whether there are differences in the expression of miR-155 and mRNA HIF1A in plasma ovarian cancer patients at the early stage compared with the advanced stage.The samples using blood plasma from ovarian cancer patients RSUP Dr. Sardjito with 32 ovarian cancer patients early stages and 20 ovarian cancer patients advanced stages. Total RNA was isolated from blood plasma samples of ovarian cancer patients. cDNA synthesis from total RNA was performed to obtain cDNA. The expression of miR-155 and HIF1A were calculated using qPCR. qPCR results were analyzed using Biorad CFX Manager Software. The analysis showed that the expression of miR-155 were 2,18 times lower (p-value = 0,018*) in the plasma of advanced stage ovarian cancer compared with early stage, the differences were statistically significant (p value≤ 0,05). Whereas the mRNA expression HIF1A were 2,46 times higher (p-value = 0,039*) in the plasma of advanced stage ovarian cancer compared with early stage, the differences were statistically significant (p value≤ 0,05). This study has proved that miR-155 expression is downregulated and followed by upregulation of mRNA expression HIF1A at an advanced stage ovarian cancer compared with early stage. Keywords: Plasma, stage ovarian cancer, microRNA-155, mRNA HIF1A
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13650
10.19106/JMedScieSup004804201620
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13650/pdf
Copyright (c) 2017 S.N. Chasanah, A.S. Fitriawan, F.K. Pukan, A.I. Kartika, R, Oktriani, A. Trirahmanto, H. Pradjatmo, T. Aryandono, S.M. Haryana
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13652
2017-11-01T02:03:53Z
bik:ART
Mitochondrial Genetics and Cancer
Malik, Safarina G.
The first modern human, the Mitochondrial Eve, was traced back to Africa about 200,000 years ago, based on the variation in the mitochondrial DNA (mtDNA). An eruption of a super volcano, Mount Toba, in Sumatra 70,000 years ago may have led to a 'nuclear winter', followed by a 1,000-year ice age. This cold snap would have made life difficult; genetic evidence indicated a sharp reduction in population size around this time, reaching approximately 10,000 individuals. Once the climate started to improve, our ancestors recovered from this near-extinction event. The population expanded, and some courageous explorers ventured beyond Africa. Around 50,000 years ago some of these brave ancestors had successfully crossed the globe to South East Asia and Australia. Some of them settled in the Indonesian archipelago, forming the first settlement of prehistoric Indonesia. The second migration happened around 10,000 years ago, where a group of hunter-gatherers followed the now-submerged river systems that once ran from mainland Asia between the modern islands of Sumatera, Java, and Borneo. Then, around 4,000 years ago the third group of ancestors arrived. This agricultural community brought along their culture of pottery, plant cultivation, and animal domestication, co-inciding with the vast spread of Austronesian languages. Therefore, it is likely that the Indonesian archipelago hosts a wide range of linguistic, ethnic and genetic diversity.1 Nowadays, the modern Indonesia is home to around 700 ethnic populations, each with distinct cultural and linguistic characteristics, representing vast genome diversity.Our ancestors’ decision to embark on a sea travel and take on its related lifestyle has influenced the development of susceptibility and resistance to various diseases observed today. During the prolonged travel, our ancestors were subjected to changes in global climate and geographic dynamic, which strongly influenced and shaped the genetic background of modern humans, including the mtDNA genome. Mitochondria, a well-adapted endosymbiotic intracellular organelles, became efficient for energy production through-out the course of evolution. They are critical for survival and proliferation of living organisms under aerobic conditions and produce ATP through oxidative phosphorylation (OXPHOS). Adaptation to new environments that favor beneficial traits may have caused genetic risk differences that influence the crucial function of the mitochondria, consequently affecting many function in the cell.2 The altered function of the mitochondria might act as an important factor for disease susceptibility across many human populations, i.e. mtDNA variation that grouped together forming a certain type/group (the mtDNA haplogroup) was reported to modulate cancer susceptibility3-5 and resistance6 in Chinese population.Cancer cells are characterized in general by a decrease of mitochondrial respiration and OXPHOS, a consequence of disruptive mtDNA mutations commonly found in cancer cells, and thus one could say that the growth of cancer cells is directly limited by energetics.7 In order to survive, cancer cells must modify their mitochondrial physiology to optimize energy production to their changing environments. There are two types of advantageous mtDNA mutation in cancer cells: mutations that impair OXPHOS and serve to stimulate neoplastic transformation, and those that facilitate cancer cell adaption to changing bioenergetics environments.8 These mtDNA mutations would eventually lead to an enhanced generation of reactive oxygen species (ROS), which can act both as mutagens and cellular mitogens, and contribute directly to cancer progression.7 Therefore, it can be concluded that mitochondrial alterations are critical for cancer initiation, promotion, and metastasis (Fig 1). Figure 1. Integrated mitochondrial paradigm to explain genetic and phenotypic complexities of metabolic and degenerative disease, aging, and cancer.Top three arrows: factors that have impact on mitochondrial OXPHOS robustness, risk for developing disease symptoms. Central oval arrows: pathophysiological basis of disease processes and the basis of disease progression. Lower five arrows: summarized disease categories and phenotypic outcomes of disturbed mitochondrial energy transformation. Bottom arrow: effect of the problematic accumulation of somatic mtDNA mutations resulting in delayed onset and a progressive course of diseases and aging. Right arrow: clinical problems that can result from reduced energy production in the most energetic tissues: the brain, heart, muscle, and kidney. Left arrow: indicates the metabolic effects of mitochondrial dysfunction, which result in the perturbation of the body’s energy balance. Lower right arrow: mitochondrial alterations are critical for cancer initiation, promotion, and metastasis. Lower left arrow: the hypothesized inflammatory and autoimmune responses that may result from chronic introduction of mitochondria’s bacteria-like DNA and N-formylmethionine proteins into the bloodstream.9
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13652
10.19106/JMedScieSup004804201628
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13652/pdf
Copyright (c) 2017 Safarina G. Malik
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13653
2017-11-01T02:30:34Z
bik:ART
ROLE OF DNA METHYLATION AS A DIAGNOSTIC BIOMARKER OF SPORADIC BREAST CANCER
Harahap, Wirsma Arif
breast cancer, epigenetic, DNA methylation, BRCA1, diagnostic marker
The initiation and progression of breast cancer have been recognized for many years to be secondary to the accumulation of genetic mutations which lead to aberrant cellular function. Genetic mutations, either inherited or sporadic, may result in the activation of oncogenes and the inactivation of tumor suppressor genes. The more recent discovery that reversible alterations in histone proteins and deoxyribonucleic acid (DNA) can also lead to tumorigenesis has introduced a novel term to the field of cancer research: epigenetics. Epigenetics refers to the study of heritable changes in gene regulation that do not involve a change in the DNA sequence. The most often studied in epigenetics of breast cancer is DNA methylation. That a promoter methylation result in transcription blockade supports the notion that cellular inhibition takes place. Compared to normal tissues, hypermethylation occurs from double to triple in cancerous ones. DNA methylation plays a crucial role in oncogenesis and is one of the hallmarks of cancer.Detection of aberrantly methylated CpG islands in promoter region of several genes in DNA sample derived from nipple aspirates, serum, or cancer tissue associated with down regulation of expression or loss of function of these genes has been associated with early stages of breast cancer, where hypermethylation of CpG island points to poorer prognosis in breast cancer. DNA methylation has been identified as signature for TNBC. Methylation of BRCA1 gene is frequently demonstrated in young, estrogen receptor-negative breast cancer patients. Methylation of specific genes is known to differ across race and socioeconomic status. BRCA1 methylation in premenopausal women with sporadic breast cancer in West Sumatra region has been higher than in Western women.DNA methylation may be used to enhance current breast cancer classification. There is such a distinction between methylation and gene expression profiles of breast cancer that not all methylation profiles fit within the same molecular subtype. Specific gene methylation profiles are identified for basal-like, luminal A and HER2-overexpressing breast cancers. A number of studies have analyzed the methylation status of BRCA1, a key player in TNBC. One study demonstrated that BRCA1 promoter was methylated in TNBC. It was discovered that the sensitivity of TNBC cell lines to PARP inhibitors was increased when BRCA1 was methylated. Concurrently, BRCA1 methylation quantity was higher in patients with complete response than in those who are non-responders of neoadjuvant chemotherapy.Epigenetics is now the cutting edge of cancer research. Advances in this field will have major implications in diagnosis, prevention, treatment of cancer, and formulation of new epigenetically targeted cancer drugs.
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13653
10.19106/JMedScieSup004804201636
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13653/pdf
Copyright (c) 2017 Wirsma Arif Harahap
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13654
2017-10-30T02:59:45Z
bik:ART
The Experience of Breast Reconstructive Microsurgery
Brahma, Bayu
Haryono, Samuel J
breast cancer; microsurgical reconstruction; oncoplastic
Autologous techniques in oncoplastic breast surgery may result in graft donor site morbidity. Microsurgery has become a new surgical modality for breast reconstruction; it is a less invasive procedure. In recent experience, we have applied microsurgical technique in oncoplastic breast procedures to minimize morbidity.We reviewed the charts of breast cancer/tumor patients with microsurgical reconstruction.From February 2013 to July 2016, we performed 36 perforator flaps for breast reconstruction. The mean age of the patients was 44.4±6.7 years old, with the median tumor size of 3.7 (1.5-20) cm. No special type of carcinoma (NST) was accounted in 25 (69.4%) cases. Oncoplastic breast conserving surgery (OPS) was the procedure of choice in 17 (47.2%) cases and mastectomy was followed by free flap in 19 (52.8%) patients. In OPS, we used various perforator flaps to cover the defect. Thoracodorsal artery perforator flap (TDAP) was the most common technique used in 8 (22.2%) cases, then lateral intercostal artery (LICAP) flap in 6 (16.7%) cases, anterior intercostal artery (AICAP) flap in 1 (2.8%) cases, and superficial epigastric artery (SEAP) flap in 2 (5.6%) cases. Deep inferior artery perforator (DIEP) free flap was the reconstruction option after mastectomy. During follow-up with the mean time of 12.7±11.4 months, there were 1 local recurrence, 2 regional and systemic metastases, and 1 death due to cerebrovascular disease. There were no flap loss after pedicle perforator reconstruction but total flap necrosis occurred in 5 patients with DIEP free flap. In one patient, we successfully salvaged the flap that had venous congestion. There was no seroma at donor site and no limitation in abdominal wall function after DIEP reconstruction.In our experience, microsurgical reconstruction in breast surgery has been a safe procedure and has less donor site morbidity. Flap failure rate may be improved by refining microsurgical technique
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13654
10.19106/JMedScieSup004804201603
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13654/pdf
Copyright (c) 2017 Bayu Brahma, Samuel J Haryono
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13655
2017-10-30T07:35:13Z
bik:ART
Oncoplasty: How to start and experience from Yogyakarta
Hardiyanto, Herjuna
oncoplasty, breast, thyroid, reconstruction
AbstractReconstruction surgery after tumor removal is increasingly essential because of changes in patient expectations and demand. With the advance of early screening and detection, our ability to detect early stage cancer is significantly improved. Reconstruction is mostly suitable in patients with early stage cancer. In addition, there is growing ability that that immediate reconstruction in particular selected patients can be performed in a single time operation combining oncological and aesthetic procedures with considerably excellent result. With the implementation of full coverage insurance by BPJS, most oncological surgery including in breast cancer will be performed by general surgeon in regency hospital. Reconstruction in selected patients usually is performed as delayed procedure by plastic surgeon. With the advance in surgical oncology training, breast surgery should be performed by surgical oncologist with specific oncoplasty training that can offer immediate reconstruction with both extensive removal tumor and reconstruction. Therefore, training in oncoplasty is very important for surgical oncologist. In Yogyakarta, we performed reconstruction after surgery of breast tumor, thyroid cancer, radical neck dissection and facial basal cell carcinoma with relatively excellent results. Further training and innovation are required to improve this early stage oncoplasty practice in Yogyakarta.Keywords :
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13655
10.19106/JMedScieSup0048042016013
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13655/pdf
Copyright (c) 2017 Herjuna Hardiyanto
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13656
2017-11-01T02:23:37Z
bik:ART
DNA extraction, Polymerase Chain Reaction, and Sequencing : Workshop in Clinical Genetics
Anwar, Sumadi Lukman
AbstractDNA extraction, Polymerase Chain Reaction (PCR), and Sequencing are basic methods in molecular biology and genetics. Those there are routinely performed as basic methods in genetic research and currently also for diagnostic lab especially for pathology and human genetics. With the advance in the genetics and clinical service for cancer management, mutation analysis is very important not only for diagnosis but also for prediction of therapeutic response. Detection of KRAS, BRAF, EGFR, and c-KIT mutations is presently performed in almost every molecular pathology lab as part of daily clinical service in cancer management. In this workshop we will discuss tips and tricks for those three basic lab methods. How to improve amount and purity of DNA extraction from blood and tissues, how to avoid DNA degradation during the procedure and storage, how to perform PCR, factors and substance that inhibit polymerases during PCR, how to design effective primer pairs, and how basic theory for sequencing, and interpretation of sequencing will be discussed. Although it has been widely discussed, this workshop is especially important for clinicians who previous do not have hands-on laboratory experience. In addition, number of labs with ability to perform and serve basic genetic and molecular analysis are still limited in Indonesia. With this workshop, we expect to improve knowledge and skill in DNA extraction, PCR, and Sequencing.Keywords : DNA, PCR, sequencing
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13656
10.19106/JMedScieSup004804201633
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13656/pdf
Copyright (c) 2017 Sumadi Lukman Anwar
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13657
2017-10-30T02:44:21Z
bik:ART
Expression of Circulating microRNA-141 and mRNA of PTEN (Phosphatase and Tensin Homolog) in Blood Plasma of Ovarian Tumor and Epithelial Ovarian Cancer Patient
Fitriawan, AS.
Chasanah, SN
Pakun, FK
Kartika, AI
Oktriani, R.
Trirahmanto, A.
Prajatmo, H.
Ghozali, A.
Aryandono, T.
Harjana, SM.
Epithelial ovarian cancer, circulating microRNA, miR-141, PTEN mRNA
Epithelial Ovarian Cancer (EOC) is the most lethal gynecological malignancies among woman. The majority of this disease is diagnosed at the advanced stage due to lack of specific symptoms and effective screening methods. Therefore, an adequate biomarker for early detection is needed and may improve patient survival. microRNA is a small non-coding RNA that regulates gene expression in post-transcriptional level. Several studies have shown the ability to detect microRNA in blood circulation so microRNA may be used as a minimally invasive biomarker for EOC. microRNA-141 (miR-141) plays a major role in EOC by regulating expression of several tumor suppressor gene. Previous study have confirmed that miR-141 regulated PTEN gene directly by interacting with 3’UTR sequence of PTEN mRNA, and upregulation of miR-141 caused downregulation of PTEN expression in vivo. PTEN is important tumor suppressor gene that its inactivation found in various human cancer. PTEN is protein with lipid phosphatase activity that negatively regulate PI3K-AKT signaling pathway, thus playing a important role in various cellular process such as proliferation, growth, cell survival, EMT, cell motility, and angiogenesis. When various studies found that PTEN mRNA and protein expression is significantly downregulated in EOC tissue, little is known about the expression of PTEN mRNA in blood circulation of EOC patient, especially in Yogyakarta population.The aims of this study is to measure and to investigate the correlation of miR-141 and mRNA PTEN expression in plasma of ovarian tumor patient and EOC patient.This study used cross-sectional design. 25 blood plasma of ovarian tumor and 25 blood plasma of EOC were collected. Total RNA was isolated and reverse transcribed to obtain cDNA. The expression of miR-141 and mRNA PTEN were measured by quantitative real-time polymerase chain reaction assay (qPCR). The 2^(-∆∆cq) method was used to calculate relative quantification of miR-141 and mRNA PTEN using miR-16 as reference gene for microRNA and beta-actin mRNA as reference genes for PTEN mRNA. Expression of miR-141 is significantly elevated in blood plasma of epithelial ovarian cancer patient compared to the ovarian tumor (p=0,001, fold change=7,59). Expression of PTEN mRNA significantly downregulated in blood plasma of epithelial ovarian cancer patient compared to the ovarian tumor (p=0,001, fold change=11,63). There was a significant negative correlation between miR-141 expression and mRNA PTEN expression in blood plasma of epithelial ovarian cancer patient (p=0,033; r=-0,428).miR-141 and mRNA PTEN differentially expressed in blood plasma of ovarian tumor and epithelial ovarian cancer patient. There was a negative correlation between miR-141 and mRNA PTEN expression in blood plasma of epithelial ovarian cancer patient. Keywords: Epithelial ovarian cancer, circulating microRNA, miR-141, PTEN mRNA
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13657
10.19106/JMedScieSup004804201602
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13657/pdf
Copyright (c) 2017 AS. Fitriawan, SN Chasanah, FK Pakun, AI Kartika, R. Oktriani, A. Trirahmanto, H. Prajatmo, A. Ghozali, T. Aryandono, SM. Harjana
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13658
2017-10-30T03:28:52Z
bik:ART
THE EXPRESSION OF Hsa-miR-21-5p AS MINIMAL INVASIVE MARKER TO ADJUVANT CHEMOTHERAPY IN BREAST CANCER PATIENTS
Tanjung, Dewi Safitri
Fitria, MS.
Kartika, AI.
Rakhmina, D.
Anwar, SL.
Oktriani, R.
Irianiwati, .
Harjana, SM.
Aryandono, T.
breast cancer; adjuvant chemotherapy; miR-21-5p; minimal invasive marker
AbstractBreast cancer remains the leading cause of death among women, and there is a need to develop minimally invasive marker. In our previous study based on clinicopathologic in pre-chemotherapy patients showed miR-21 was upregulated 1.32 times higher at advanced stage compared with early stage. Therefore the matched patients for post-chemotherapy samples were used. The aim of this research is to examine the expression of miR-21 as potential marker to adjuvant chemotherapy in breast cancer patients. The samples were taken by using cross sectional method with total 39 blood plasma samples from breast cancer patients in adjuvant chemotherapy and 12 healthy control samples. Plasma was obtained from blood samples and then RNA isolated were performed. Total RNA was reverse transcribed using cDNA synthesis. The expression of miR-21 was then analyzed using specific primer for miR-21 and miR-16 as the reference gene. Livak Method was used to calculate the expression level in each group. The result showed that there is significant downregulated expression of miR-21 in postchemotherapy 2.61 fold compared with pre-chemotherapy (p<0.05). The expression of miR-21 upregulated 2.2 folds (p<0.05) in pre-chemotherapy compared with healthy control, while in post-chemotherapy compared with healthy control, the expression of miR-21 was 0.8 fold (p<0.05). In conclusion, Hsa-miR-21-5p can be used as marker for adjuvant chemotherapy response in breast cancer because there is significant different expression between prechemotherapy, post-chemotherapy and healthy control. The continuation research in the near future for detecting the expression of tumor suppressor protein regulated by miR-21 is needed.Keywords: breast cancer, adjuvant chemotherapy, miR-21, minimal invasive marker
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13658
10.19106/JMedScieSup004804201605
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13658/pdf
Copyright (c) 2017 Dewi Safitri Tanjung, MS. Fitria, AI. Kartika, D. Rakhmina, SL. Anwar, R. Oktriani, . Irianiwati, SM. Harjana, T. Aryandono
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13678
2017-10-31T03:03:04Z
bik:ART
The Expression of hsa-miR-155-5p in Plasma Samples Of Breast Cancer Before And After Chemotherapy
Fitria, Meutia Srikandi
Haryana, Sofia Mubarika
Anwar, Sumadi Lukman
Aryandono, Teguh
Tanjung, Dewi Sahfitri
Kartika, Aprilia Indra
Oktriani, Risky
Irianianiwati, .
Sari, Dwi Nur Indah
Breast cancer has emerged as the most common cancer-related mortality among women worldwide. Therefore, early cancer detection using biomarkers such as microRNA is needed. One of microRNAs that has an important role in breast cancer development is miR-155. Hsa-miR-155-5p is an oncomir that is commonly dysregulated in breast cancer. This study aims to determine the expression of hsa-miR-155-5p in breast cancer patient’s plasma before and after chemotherapy. We collected 64 samples from breast cancer patients admitted to Dr. Sardjito Hospital in Yogyakarta. RNA from plasma was extracted using RNA Isolation Kit miRCURY-Biofluid. cDNA synthesis was performed using cDNA Synthesis kit II and quantification of miR-155-5p using ExiLent SYBR Green master mix (Exiqon). qRT-PCR results were then analyzed with Livak's method and compared (before and after chemotherapy) with t-test. Expression of miR-155-5p in the breast cancer patients’ plasma after chemotherapy was significantly increased (10.59 times) when compared to before chemotherapy (p = 0.001). We concluded that there was upregulated expression of miR-155-5p after chemotherapy than before chemotherapy. There has not been a known, relevant pathway between hsa-miR-155-5p and chemotherapy regimens nor resistance to chemotherapy. Keywords: Breast cancer, plasma, hsa-miR-155-5p, oncomiR, chemotherapy.
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13678
10.19106/JMedScieSup0048042016018
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13678/pdf
Copyright (c) 2017 Meutia Srikandi Fitria, Sofia Mubarika Haryana, Sumadi Lukman Anwar, Teguh Aryandono, Dewi Sahfitri Tanjung, Aprilia Indra Kartika, Risky Oktriani, . Irianianiwati, Dwi Nur Indah Sari
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13680
2017-10-31T05:56:45Z
bik:ART
miR-21, miR-29c, and miR-155 as Biomarker to Develop Minimal Invasive Diagnostic in Hepatocellular Carcinoma Patient
Lestari, P
Qoriansas, N
Tanjung, D.S
Fitria, M.S.
Kartika, A.I.
Wardana, T.
Ratnasari, N.
Harjana, S.M.
Aryandono, T.
Background: Hepatocellular carcinoma (HCC) is one of the most prevalent malignancies and the third leading cause of cancer-related deaths worldwide. Incidence and mortality of HCC increase annually because of its poor prognosis. The most common cause of this condition is its characteristics with high metastasis and high recurrence after surgical treatment. So, it is become important to find new serological biomarkers for early stage detection of HCC. Plasma microRNA are being actively investigated as minimal invasive biomarkers in human cancers, including HCC.Objective: The aim of this study is to investigate the level expression of miR-21, miR-29c, and miR-155 as novel serological biomarkers for hepatocellular carcinoma.Methods: This study is quasi experimental and remain as preliminary study. Collecting for more samples is currently underway. HCC patient blood samples obtained from RSUP dr. Sardjito Yogyakarta based on specific inclusion and exclusion criteria. Blood samples were collected from 8 patients and 8 healthy controls. The collected blood samples were treated as follows: plasma isolation, RNA total isolation, cDNA synthesis, quantification by qRT-PCR, data analysis with Biorad CFX ManagerTM Softwere to determine Cq, followed by the calculation of expression levels using Livax Methods.Result: The result showed that miR-21 and miR-155 were upregulated 1.68 fold and 2.38 fold respectively compared with healthy control, while miR-29c was downregulated 3,45 fold compared with healthy control.Conclusion: Based on the result of preliminary study, it can be concluded that miR-21 act as oncomiR, while miR-29c and miR-155 act as tumor supressor miR in HCC. The three microRNAs can be detected in HCC and can be used as minimal invasive biomarkers to detect HCC.Keywords: HCC, miR-21, miR-29c, miR-155, minimal invasive, biomarker
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13680
10.19106/JMedScieSup0048042016019
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13680/pdf
Copyright (c) 2017 P Lestari, N Qoriansas, D.S Tanjung, M.S. Fitria, A.I. Kartika, T. Wardana, N. Ratnasari, S.M. Harjana, T. Aryandono
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13687
2017-11-01T02:22:27Z
bik:ART
DNA methylation and expression of Homeobox gene family as diagnostic and prognostic markers in human hepatocellular carcinoma
Anwar, Sumadi Lukman
Lehmann, Ulrich
homeobox, DNA methylation, expression, hepatocellular carcinoma, survival
ABSTRACTHomeobox genes consist of a family of evolutionarily conserved genes that play important roles in morphogenesis, embryogenesis, and cell fate determination. Re-expression of embryogenic genes has been associated with carcinogenesis of human cancers. Aberrant expression of homeobox genes has been increasingly found to modulate diverse processes such as cell proliferation, cell death, metastasis, angiogenesis and DNA repair. We studied DNA methylation and expression of homeobox gene family in human hepatocellular carcinoma (HCC), the fifth most common cancer and the third leading cause of cancer mortality worldwide. We performed microarray for comprehensive DNA methylation and gene expression using primary HCC samples and healthy liver tissues. Confirmation using pyrosequencing and RT-PCR was then performed. Clustering both unsupervised and supervised methods using Qlucore software was then performed. Enrichment of homeobox genes both for DNA methylation and gene expression could differentiate HCC and the healthy liver tissues. Profile of homeobox gene methylation could further predict clinical outcome. Inverse correlation between DNA methylation and gene expression was shown (HOXA9, Spearman r=-0.49, p=0.002). Gain of DNA methylation in HOXA9, HOXA13, and MEOX1 correlated with shorter HCC survival (log-rank Mantel-Cox test p=0.02, with median survival 50 and 490 weeks, respectively). We demonstrated potential roles of DNA methylation and gene expression profiles of Homeobox gene family as diagnostic and prognostic marker in patients with HCC.
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13687
10.19106/JMedScieSup004804201632
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13687/pdf
Copyright (c) 2017 Sumadi Lukman Anwar, Ulrich Lehmann
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13688
2017-10-30T08:23:16Z
bik:ART
The Expression of Homo Sapiens microRNA-21 (Hsa-miR-21-5p) and mRNA Reversion Inducing Cysteine Rich Protein with Kazal Motifs (RECK) in Plasma of Epithelial Ovarian Cancer
Kartika, Aprilia Indra
Chasanah, SN
Fitriawan, AS
Tanjung, DS
Ftria, MS
Pakun, FK
Oktriani, R
Trirahmanto, A
Prajatmo, H
Aryandono, T
Haryana, SM
Hsa-miR-21-5p, EOC, plasma microRNA, mRNA RECK, microRNA expression in cancer and healthy
ABSTRACT Epithelial Ovarian Cancer (EOC) is malignant cancer that caused death for most women in Indonesia. The emergence of EOC showed no specific symptoms in its early stages; that makes the screening mostly occur when patients are in advanced stage. Treatment of EOC at an advanced stage will be more challenging with poor prognosis. Therefore, minimally invasive biomarkers are needed to diagnose at the early stage. microRNA is one of the potential biomarkers which not only expressed inside the cell but also secreted outside the cell with exosome protection. This protection makes microRNA stable. Moreover, several studies have shown the ability to detect microRNA in the blood sample. microRNA-21 (miR-21) is oncomiR which targeted tumor suppressor mRNA RECK based on in silico analysis.The first aim is to determine the expression of miR-21 in plasma samples of EOC patients compared with healthy controls. The second aim is to investigate the expression correlation between miR-21 and RECK mRNA.Blood samples were collected from 30 patients and 30 healthy controls. Plasma was then obtained from centrifugated blood samples. The total RNA was isolated and reverse transcribed to produce cDNAs. cDNAs were then quantified using qPCR using specific primer for miR-21 and RECK mRNA. The expression analysis was done relative expression method by Livak. The expression of miR-21 was calculated using the miR-16 expression as the reference gene. Also, Beta-actin was used as reference gene for RECK mRNA calculation. The correlation between the expression of miR-21 and mRNA RECK was analyzed using the Spearman rho correlation analysis.In this study showed the expression of miR-21 in patients with EOC increased 4.7579-fold compared with healthy controls (p <0.05). On the other hand, the miR-21 target, RECK mRNA, decreased 4,2 times with fold change 0.237665 on plasma EOC patients compared with healthy controls (p <0.05). The statistical calculation of the expression of miR-21 and mRNA RECK was inversely proportional to mRNA RECK with a strong correlationThis study has been able to prove that the expression of miR-21 is up-regulated in EOC patients and confirmed by the down-regulation of RECK expression. The next research challenge is to make anti-miR-21 to suppress the expression of miR-21 in EOC, which can be analyzed the effect of miR-21 in the development of EOC.
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13688
10.19106/JMedScieSup0048042016015
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13688/pdf
Copyright (c) 2017 Aprilia Indra Kartika, SN Chasanah, AS Fitriawan, DS Tanjung, MS Ftria, FK Pakun, R Oktriani, A Trirahmanto, H Prajatmo, T Aryandono, SM Haryana
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13690
2017-10-30T03:19:49Z
bik:ART
Optogenetics, A Futuristic Panacea in Genetics
Anurogo, Dito
Ikrar, Taruna
optogenetics; opsins; neuropsychocardioncology; SSFO; DREADD; TCU
AbstractOptogenetics is the constellation of optics, genetics and bioengineering which unites genetic engineering with optics to notice and manage the function of genetically targeted groups of cells with light, often in the intact animal, via light-sensitive microbial membrane proteins (opsins). Light-sensitive genes specifically including the genetically targeted light-gated channels channelrhodopsin-2 (ChR2) and halorhodopsin (NpHR) result in intracellular ion flow during optical illumination. Afterward, the neurons encounter a series of changes resulting from membrane depolarization or hyperpolarization. Although the rooted origins of optogenetics is from neuroscience, it can be potentially applied in neuropsychocardioncology (neurology, psychiatry, oncology, and cardiology).This critical review will explicate a comprehensive summary of the roles of optogenetics in the field of neuropsychocardioncology.Optogenetics can be potentially developed as neuroprosthetics and direct NpHR in the management of spastic movement disorders. Optogenetics can control of larynx muscle contraction in vivo, using both transgenic ChR2 expressing mice and viral transduction of muscle.In epilepsy, the efficacy of optogenetics is proved. Pyramidal cells in the cortex were transduced with halorhodopsin, and photoinhibition of the neurons decreased electrical seizure activity. Optogenetic and DREADD technologies are in their early stages, particularly with respect to PD research or therapy.In autism and schizophrenia, behavioral deficits may arise from elevation in the cellular balance of excitation/inhibition (E/I balance) within neuronal microcircuits. This hypothesis was tested by optogenetically elevating the E/I balance in the medial-prefrontal cortex using a step-function opsin (SSFO), together with red-shifted opsins (C1V1). Increased excitation in excitatory pyramidal neurons, lead to social-cognitive dysfunctioning which are similar to those seen in autism. Cortical gamma oscillations are an indicator of enhanced information processing, which is highly affected in schizophrenic patients.Using optogenetic technology, researchers divulge the characterization of phosphatidylinositol 3-kinase (PI3K) in Rac1-dependent lamellipodial motility in PC-3 prostate cancer cells. PI3K, acting downstream of Rac1, has an important role in the initiation of lamellipodial extension, which underlies prostate cancer cell invasion and metastasis. As in Parkinson, human cells can be engineered to deliver the excitatory (hM3Dq receptor) and/or the inhibitory (hM4Di receptor) form so that cellular activity may be turned up or down.The optogenetic TCU (tandem-cell-unit) strategy can be valuable in appraising tissue graft integration and cell delivery in the myocardium during cardiac tissue repair procedures. Low-energy pacing strategies can be srutinized by optogenetic investigations. Specifically, optical stimulation can be aimed for strategic structures of the conduction system. Optogenetic studies have already contributed to a better understanding of the neural circuits affected in many disorders. A conceptual and mutual understanding of multidisciplinary approaches and collaboration will enable researchers, clinicians, stakeholders, government develop and apply optogenetics in comprehensive medical services and health care.Keywords: optogenetics, opsins, neuropsychocardioncology, SSFO, DREADD, TCU.
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13690
10.19106/JMedScieSup004804201604
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13690/pdf
Copyright (c) 2017 Dito Anurogo, Taruna Ikrar
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/13691
2017-10-31T05:48:58Z
bik:ART
Hormonal Contraceptive Use as Risk Factor for Breast Cancer in Young Javanese Women
Kusuma, Luna Fitria
Yarso, Kristanto Yuli
Previous study from 351 Indonesian women shown that they had breast cancers at younger age compared with western. In this study we investigate role of hormonal contraceptive as risk factor for Indonesian Javanese young breast cancer cases. However, the presence different life style between ethnic alter their risk as causal factors across populations. Diagnostic and prognostic study findings, including breast cancer prediction rules, must therefore be validated in Asian women. We undertook case-control study to determine population-based distributions of breast cancer among young Javanese people, one of the largest populations in Indonesia (Southeast Asia). A total of 500 women diagnosed with breast cancer participated in this study, divided in to two group young (less 40 years old) and mature breast cancer. Data for hormonal contraceptive, clinico-pathological characteristics and other risk factors were collected. We found that young Javanese women who use hormonal contraceptive for more than 10 years had a 4,67 fold increased risk of being diagnosed with breast cancer in young age (p<0,01). We didn’t found any differences between this two groups in menarche and parity. Interestingly for Javanese women who breast feeding more than 18 months increase 1,74 fold increased risk of being diagnosed with breast cancer in young age (p<0,01).
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/13691
10.19106/JMedScieSup0048042016017
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/13691/pdf
Copyright (c) 2017 Luna Fitria Kusuma, Kristanto Yuli Yarso
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/15621
2019-08-05T06:59:10Z
bik:ART
Surgical outcome of scoliosis in Marfan syndrome: a case series report
Irianto, Komang Agung
Wardana, Gestana R
scoliosis - Marfan syndrome – quality of life - questionnaire SF-36 – Cobb angle
original article
Scoliosis in marfan syndrome (MFS) manifests on 60% patients. Moreover, the scoliosisnoticeable in earlier age is more progressive, refracted, and rigid compared to idiopathicadult scoliosis. The surgical correction provides notorious higher perioperative risk,whereas conservative treatment using brace is not effective to prevent progressivityof the scoliosis. In this a case report, we reported the surgical outcome of MFS scoliaispatients with MFS who operated using posterior fusion instrumentation by mean of thequality of life SF-36 questioner. This was a retrospective case series involving five MFSscoliosis patients who underwent posterior fusion instrumentation with initial Cobb angleof 87.417.57o and initial kyphotic angle of 32.8 ± 14.52o. Clinical, radiological andquality of life of the patients based on SF-36 questionnaire were evaluated within 6-36months follow up. Post-operative showed the Cobb angle become 46.2 ± 16.3o and thekyphotic angle become 21.6 ± 9.94o. No intraoperative or post-operative complicationswere observed. After 6-36 months follow up, the Cobb angle became 45.2 ± 17.48oand the kyphotic angle became 21.6 ± 9.94o. In addition, all patients had physical andmental health scored similar to 2 years post-surgery scoliosis scoring according to SF-36 orthopedic scoring guidelines. I conclusion, the surgical outcome of posterior fusioninstrumentation in MFS scoliosis showed good correction of Cobb angle and Kyphoticangle. The quality of life of the patients based on physical and mental health questionnaireis satisfactory.
Universitas Gadjah Mada
2018-04-04
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/15621
10.19106/JMedSci005002201814
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 2 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 2 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/15621/pdf
Copyright (c) 2018 komang agung irianto, Gestana R Wardana
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/15937
2019-08-05T07:20:12Z
bik:ART
Prolonged Kidney Ischemia-Reperfusion Injury Associates with Inflammation, Vascular Remodelling, and Myofibroblast Formation
Arfian*, Nur
Ats-tsani, Hilma Kholida
Sayekti, Pratiwi Indah
Lakabela, Dwina Agrila
Amelia, Amelia
Febriyanto, Toni
Antonio, Hana Rutyana Putri
Wibisono, Dian Prasetyo
Sari, Dwi Cahyani Ratna
ischemia; reperfusion injury; kidney; inflammation; vascular remodelling; myofibroblast
Prolonged kidney ischemia-reperfusion injury (IRI) is the important risk factor for leading to chronic kidney disease (CKD). Persistent hypoxia and inflammation are considered as the main pathogenesis of chronic injury, followed by myofibroblast expansion and fibrosis process. Tubular injury, cell proliferation, and vasoconstriction, as acute compensatory responses, are restored in chronic phase. The aim of the study was to investigate the relation between inflammation, vascular remodeling, and myofibroblast formation as response to ischemia injury after prolonged kidney ischemia-reperfusion (I/R). Fifteen male Swiss mice aged 3-4 months were used as kidney I/R injury model after bilateral pedicle renal clamping. Rats were divided into 3 groups with five rats in each group i.e. control group (sham operation/SO), acute I/R model (IR1), and chronic I/R model (IR12). PAS staining was used for scoring tubular injury. Fibrosis was assessed using sirius red and a-SMA immunostaining for myofibroblast expansion. PCNA and CD68 immunostaining were used for identifying cell proliferation and macrophage infiltration. RT-PCR was conducted for assessing MCP-1, HIF-1a, and ppET-1 expression, which were quantified using ImageJ software. Data were analyzed using one way ANOVA and Kruskal-Wallis test with significance level of p<0.05. Significantly increase of tubular injury score (p<0.001) and PCNA positive cell (p<0.001) in IR1 group compared to SO were observed, otherwise HIF-1a of IR12 enhanced (p<0.05). Macrophage cell count (p<0.01) and MCP-1 expression (p<0.05), were significantly increase in IR1 and IR12 injury, compared to SO. Wall thickness of arteries was significantly increase (p<0.05) as well as decrease of vascular lumen area (p<0.05), followed by enhancement of ppET-1 expression (p<0.01) in IR1 group and restored significantly (p<0.05) in IR12 group. Fibrosis fraction-area and myofibroblast expansion were significantly increase gradually from IR1 to IR12 injury (p<0.01). In conclusion, prolonged kidney I/R injury induces the sustainability of hypoxia and inflammatory response, which promotes myofibroblast formation, and decrease the response of vascular remodelling.
Universitas Gadjah Mada
PUPT (Penelitian Unggulan Perguruan Tinggi)
2018-03-26
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/15937
10.19106/JMedSci005001201801
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 1 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 1 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/15937/1
Copyright (c) 2018 Nur Arfian*, Hilma Kholida Ats-tsani, Pratiwi Indah Sayekti, Dwina Agrila Lakabela, Amelia Amelia, Toni Febriyanto, Hana Rutyana Putri Antonio, Dian Prasetyo Wibisono, Dwi Cahyani Ratna Sari
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/16647
2018-11-16T02:51:10Z
bik:ART
Sepsis risk factor in mount Merapi eruption victims with 2nd or 3rd degree of burn injury
Zega, Yamoguna
Dachlan, Ishandono
human saliva – aloe vera – natrium chloride – 2nd degree burn injury
Sepsis is one of the fatal complications of burns. It is the most common cause of deathfrom burns. Burn sepsis usually occurs after a burn injury develops into infection. Thisstudy was conducted to determine correlation between age, burn area, inhalation trauma,enteral nutrition start time, escarotomy time, and albumin level of sepsis in patientswith 2nd or 3rd degree burn injury. This was a cross sectional study during May to June2012. The subjects in this study were 39 victims of mount Merapi eruption in 2010 whosuffered 2nd or 3rd degree of burns injury. The result showed the correlation betweenwidespread burns, inhalation trauma, time of escarotomy, and albumin levels with sepsis(p < 0.05). In conclusion, the risk factor for sepsis in patients with 2nd or 3rd degree ofburns injury are the area of burns (> 50%), inhalation trauma, time of escarotomy (> 72hours), and albumin levels (<3.5g/dL).
Universitas Gadjah Mada
2018-03-26
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/16647
10.19106/JMedSci005001201810
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 1 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 1 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/16647/pdf
Copyright (c) 2018 Yamoguna Zega, Ishandono Dachlan
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/16719
2018-07-23T03:30:51Z
bik:ART
Effect of exercise on lipid peroxidation in student soccer players
Puspaningtyas, Desty Ervira
Afriani, Yuni
Mahfida, Silvi Lailatul
Kushartanti, Wara
Farmawati, Arta
soccer – exercise - O2max - lipid peroxidation – malondialdehyde
student soccer players
Training is conducted to improve physiological functions that can support improvementof cardio-respiratory function (O2max). However, intensive training can lead to oxidativestress, which can contribute to health problems. The purpose of this study was to evaluatethe effect of training on serum lipid peroxidation levels in student soccer players. Thestudy was pre-experimental study with a one-shot case design conducted in April 2014.Twelve student soccer players from UGM who chosen by purposive sampling and metthe inclusion and exclusion criteria were involved in the study. Each subject receivedtraining in the form of O2max measurements using the yo-yo intermittent recovery test.Plasma malondialdehyde (MDA) levels were measured using the thiobarbituric acidmethod 30 min after O2max measurement. Pearson correlation was used to analyzethe correlation between O2max and plasma MDA levels. The mean age of subject was19.25±1.06 years old. Subjects had normal nutritional status (body mass index 20.99± 1.65) with mean body weight of 58.13±3.76 kg and mean height of 166.2±3.40cm. The mean O2max score was 49.56±0.61 mL/kg/min. The mean plasma MDA levelwas 4.32±2.09 μmol/L. There was no significant correlation between O2max and plasmaMDA levels (p=0.7717). In conclusion, training does not negatively impact oxidativestress conditions in student soccer players.
Universitas Gadjah Mada
2018-03-26
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/16719
10.19106/JMedSci005001201811
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 1 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 1 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/16719/pdf
https://jurnal.ugm.ac.id/bik/article/downloadSuppFile/16719/871
Copyright (c) 2018 Desty Ervira Puspaningtyas, Yuni Afriani, Silvi Lailatul Mahfida, Wara Kushartanti, Arta Farmawati
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/16920
2019-10-31T02:47:45Z
bik:ART
Differentiation of body fat composition between skinfold caliper (SKF) and bioelectrical impedance analysis (BIA) methods among professors
Zhe Yiin, Nicole Ngo
Lubis, Leonardo
Luftimas, Dimas Erlangga
bioelectrical impedance analysis - body fat percentage – professors - skinfold caliper – body mass index
Body fat composition reflected by body fat percentage (BF%) is one of the important components in disease risk evaluation. Among the methods available to measure BF%, skinfold calipers (SKF) and bioelectrical impedance analysis (BIA) are the most commonly used. The study was conducted to evaluate the difference in body fat composition measurement between SKF and BIA methods among professors. This study analyzed secondary data from the health evaluation of Universitas Padjadjaran (UNPAD) professors. This study involved 72 professors (50 male, 22 female) after fitted into the inclusion and exclusion criteria. BF% was measured among UNPAD professors using SKF and BIA. After obtaining agreement by Bland-Altman Plot, the data were analyzed by muliple paired-t test according to gender, physical activity level and body mass index (BMI) categories. The study showed no significant difference in BF% between SKF and BIA in overall population, between gender and physical activity level (p>0.05). For BMI, the only category that showed significant difference in BF% between the 2 methods was obese I group (p=0.001). In conclusion, there is no significant difference in BF% between SKF and BIA methods according to gender, physical activity levels and BMI categories except for obese I group.
Universitas Gadjah Mada
2019-01-28
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/16920
10.19106/JMedScie/005004201809
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 4 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 4 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/16920/pdf
https://jurnal.ugm.ac.id/bik/article/downloadSuppFile/16920/897
https://jurnal.ugm.ac.id/bik/article/downloadSuppFile/16920/898
Copyright (c) 2019 Nicole Ngo Zhe Yiin, Leonardo Lubis, Dimas Erlangga Luftimas
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/16949
2019-05-21T07:13:11Z
bik:ART
Correlation between haemoglobin concentration and oxygen saturation (SpO2) in elderly professors
Suherlim*, Della Aprilliana
Permana, Hikmat
Lubis, Leonardo
hemoglobin - oxygen saturation – professors – elderly - immunity
The aging process is recognized by the decreases of the body functions which is caused by molecular and cellular damages. The population which is mostly affected by this aging process is elderly. In elderly, it is most likely that tissue oxigenation decreases due to the reduction of haemoglobin level and oxygen saturation (SpO2).This study aimed to evaluate the correlation between the hemoglobin concentration and SpO2 in the elderly professors in Universitas Padjadjaran (UNPAD), Bandung. This was cross sectional study using secondary data from medical records of the elderly professors. One hundred and eight data of the professors (84 males and 24 females) who met the inclusion and exclusion criteria were used in this study.Spearman’s Rank correlation test was used to analyze the data of total subjects and male professors group, while Pearson correlation test was used to analyze the data of female professors group. Correlation test between hemoglobin concentration and SpO2 resulted in an insignificant results in male professors (r = 0.028; p = 0.801), female professors (r = 0.132; p = 0.538), and total subjects (r = 0.036; p = 0.712). In conclusion, there is no correlation between hemoglobin concentration and SpO2 in the elderly professors of UNPAD, Bandung.
Universitas Gadjah Mada
Leonardo Lubis, Universitas Padjajaran, Department of Anatomy, Physiology, and Cell Biology
2018-10-05
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/16949
10.19106/JMedSci005002201804
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 2 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 2 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/16949/pdf
Copyright (c) 2018 Della Aprilliana Suherlim*, Hikmat Permana, Leonardo Lubis
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/17066
2019-08-05T07:20:12Z
bik:ART
Correlation between CD4 cell counts with mucocutaneous manifestations: study of HIV patients in Dr. Sardjito General Hospital, Yogyakarta
Pudjiati, Satiti Retno
Dewi, Nadia Akita
Palupi, Sekar Sari Arum
CD4 cell count; mucocutaneous manifestations; HIV; opportunistic infection; fungal infection
Mucocutaneous disorders often seen in HIV patients with varying morbidity. The HIVprogression is characterized by the declining of CD4 cell counts and emergence ofmucocutaneous manifestations. The aim of the study was to evaluate the relationshipbetween CD4 cell counts with mucocutaneous manifestations in HIV patients. This wasa cross-sectional study based on medical records at Dr. Sardjito General Hospital duringthe period January 2011-December 2015. Data of patient’s age, sex, risk factors oftransmission, most mucocutaneous manifestations and CD4 cell count were gathered. Thecorrelation between CD4 cell counts with muscocutaneous manifestations were analyzedusing chi-square test. A total 928 patients were involved in the study. More than half ofthe patients were male (65.4%) and mostly, the patients aged 20-29 years (38.69%).The main risk factors for HIV transmission were unsafe sex (75%). The highest CD4 cellcounts was 1094 cells/mm3 and the lowest was 1 cell/mm3. We found 306 cases ofmucocutaneous manifestations. The most mucocutaneous manifestations was a fungalinfection (40.4%) with the highest infection type was oral candidiasis(33.8%); then noninfection(28%) with the highest type was drug eruption(35.9%); and tumors(0.5%) thatwas only Kaposi sarcoma cases. We also found sexually transmitted infections (STIs)(18.85%) with the highest cases was condyloma acuminata (49.3%). Statistical analysisshowed a significantly relationship between CD4 cell counts with a fungal infection(p <0.0001; OR= 3.8; 95% CI: 2.29 - 6.30), viral infection (p =0.0031; OR= 0.4;95% CI: 0.24-0.74) and parasitic infection (p=0.043; OR= 0.2; 95% CI: 0.06-0.61).In conclusion, alteration in CD4 cell counts affects opportunistic infections occurencein HIV patients. Lower CD4 cell counts (<200 cells/mm3) increases the risk of fungalinfection as much as 3.8 times. Higher CD4 cell counts (>200 cells/mm3) increases therisk of viral infection by about 2.5 times and parasitic infections as much as 5 times.
Universitas Gadjah Mada
2018-03-26
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/17066
10.19106/JMedSci005001201805
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 1 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 1 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/17066/pdf
Copyright (c) 2018 Satiti Retno Pudjiati, Nadia Akita Dewi, Sekar Sari Arum Palupi
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/17118
2019-05-21T07:10:18Z
bik:ART
The effects of ethanolic extract of Phaleria macrocarpa (Scheff.) Boerl leaf on macrophage phagocytic activity in diabetic rat model
Lestari, Ira Cinta
Ghufron, Muhammad
Herwiyanti, Sri
Sumiwi, Yustina Andwi Ari
diabetes mellitus; peritoneal macrophage; phagocytosis; M1 macrophage; M2 macrophage
Diabetic patients suffer inflammation and immune deficiency as a consequence of the decrease in macrophage phagocytic activity, thus making them vulnerable to infection. The ability of Ethanolic Extract of Phaleria macrocarpa Leaf (EEPML) to increase macrophage phagocytic activity has also a potential in the diabetic case. EEPML also has anti-inflammatory effect. In this study the EEPML potential to increase peritoneal macrophage phagocytic activity and change M1 and M2 macrophage percentage in diabetic rat model is investigated. This was a quasi experimental study with post test only control group design. Fourty five male Sprague Dawley rats within the age of 8 weeks were classified into normal control group, diabetic control group with solvent, diabetic with 7mg/200g, 14mg/200g, and 28mg/200g of EEPML peroral administration, once a day. The diabetic rat model was made with streptozotocin and nicotinamide injection. The rats were terminated in 3rd, 14th and 25th day of extract administration. Peritoneal fluid was isolated then cultured for macrophage phagocytic activity assay with latex beads. M1 and M2 macrophage percentage was analyzed using flow cytometry with anti CD40 and CD206 antibody. Result of statistical analysis show that active macrophage and phagocytic index mean of EEPML rat groups on day 3, 14 and 25 was significantly higher than the control group. The mean of M1 macrophage percentage of EEPML rat groups was significantly higher than control on day 3 and 14, and lower on day 25, while mean of M2 macrophage percentage didn’t show any significant difference within groups. Conclusion of this study is administration of EEPML increases peritoneal macrophage phagocytic activity on day 3, 14 and 25. This is also increases M1 macrophage percentage on day 14, decrease M1 macrophage percentage on day 25, and doesn’t change peritoneal M2 macrophage percentage in diabetic rat model.
Universitas Gadjah Mada
2018-06-28
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/17118
10.19106/JMedSci005002201802
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 2 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 2 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/17118/pdf
Copyright (c) 2018 Ira Cinta Lestari, Muhammad Ghufron, Sri Herwiyanti, Yustina Andwi Ari Sumiwi
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/17194
2019-08-08T05:40:18Z
bik:ART
The expression of COX-2 and iNOS in ethanol and aspirin induced gastric ulcer rat models
usman, sherly
Paramita, Dewi Kartikawati
Sumiwi, Yustina Andwi Ari
gastric mucosal damage - gastric ulcer - aspirin - ethanol - COX-2 - iNOS
medical sciences
Aspirin or ethanol induced gastric ulcer rat models are the most frequently used in studies.Aspirin and ethanol induced gastric ulcers through different pathways involving COX-2 andiNOS. The aim of this study was to examine the expression of COX-2 and iNOS in gastriculcer rat model induced by ethanol and aspirin. Twenty-one Sprague Dawley rats weredivided into 7 groups i.e. control group (CA), ethanol 1st day (ED1), ethanol 3rd day (ED3),ethanol 5th day (ED5), aspirin 4th day (AD4), aspirin 6th day (AD6), and aspirin 8th day (AD8).Oral administration of aspirin was at 200mg/kgBW and the 100% ethanol at 1mL/200gBW.Macroscopic and microscopic observations were done to examine the gastric mucosaldamage, COX-2 and iNOS expressions. Severe gastric ulcers were observed in ED1and AD4 groups and mild gastric mucosal damage was observed in ED3, ED5, AD6 andAD8 groups. Microscopically, light erosion was shown by the CA and AD8 groups. Erosionwas also shown by ED3, ED5, and AD6 groups. The most severe damage with ulcers andheavier bleeding were shown by the ED1 and AD4 groups. Weak COX-2 expression wasfound in the CA, while the highest COX-2 expression was found in the ED1. The iNOSexpression in the ethanol groups was still increasing until the 5th day (ED5). In the aspiringroups, it reached the peak on the 3rd day (AD6), and already declined on the 5th day (AD8).In conclusion, the damage process of ethanol induced gastric ulcer occurred faster thanthat by aspirin. The highest COX-2 expression in the ethanol and aspirin groups wereshown at the onset begin. iNOS expression in ethanol induced ulcer groups still increaseduntil the 5th day, while in the aspirin induced ulcer groups already declined in the 5th day.
Universitas Gadjah Mada
2019-02-11
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/17194
10.19106/JMedScie/005003201807
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 3 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 3 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/17194/pdf
Copyright (c) 2019 sherly usman, Dewi Kartikawati Paramita, Yustina Andwi Ari Sumiwi
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/17575
2019-08-08T05:40:18Z
bik:ART
Profile of traumatic brain injury (TBI) in relation with maxillofacial and thoracic injury Dr. Hasan Sadikin General Hospital, Bandung
Pathmanathan, Padmini Laxmi
Alpharian, Gibran Tristan
Sutiono, Agung Budi
traumatic brain injury -maxillofacial - thoracic injury – accident – Glasgow Coma Scale
Traumatic brain injury (TBI) has a relation with concomitant injuries, which are mostlymaxillofacial and thoracic injury. This study aimed to know which injury was common inTBI patients and the difference in the severity of TBI when patients were associated withone or both types of injuries. This was a descriptive retrospective study. The data used wasmedical records from Department of Neurosurgery, Dr. Hasan Sadikin General Hospital,Bandung, Indonesia from the period of August 2015 to July 2016. Total sampling methodwas used in this study. The variables were patients with TBI, maxillofacial injury, thoracicinjury, accident related mechanism and non-accident related mechanism and the GlasgowComa Score (GCS). The total cases for this study were 47 patients. The highest case wasmales with 37 cases and 10 for females. Accident related trauma had 23 cases whereasnon accident related trauma had 24 cases. The total cases of maxillofacial injury were 32,thoracic were 6 cases whereby for both injuries were 9 cases. Patients with mild TBI were28 cases, moderate TBI were 13 cases, and severe TBI had 6 cases in total. The rate ofTBI was higher in single injury which was the maxillofacial injury. However, the thoracicand both injuries combined had higher severity of TBI compared to maxillofacial injury.
Universitas Gadjah Mada
2018-10-05
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/17575
10.19106/JMedScie/005003201808
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 3 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 3 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/17575/pdf
https://jurnal.ugm.ac.id/bik/article/downloadSuppFile/17575/1021
https://jurnal.ugm.ac.id/bik/article/downloadSuppFile/17575/1023
Copyright (c) 2018 Padmini Laxmi Pathmanathan, Gibran Tristan Alpharian, Agung Budi Sutiono
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/17610
2019-03-11T04:22:12Z
bik:ART
Relationship Between Pfmdr1 Gene Polymorphisms with Therapeutic Respons of Artesunate-Amodiaquine in Uncomplicated Falciparum Malaria Patients in Puskesmas Hanura, Pesawaran Regency, Lampung
Carolia, Novita
Mustofa, .
Kristin, Erna
ABSTRACTBackground: The failure of artesunate-amodiaquine therapy in chloroquine resistant patients have been reported in several areas in Indonesia. One of the unexpected resistance mechanisms is associated with pfmdr1 gene polymorphisms. This study determine the frequency of pfmdr-1 polymorphisms and examine the relationship of pfmdr1 gene polymorphism with therapeutic responses to artesunate-amodiaquine therapy in uncomplicated falciparum malaria patients in Puskesmas Hanura, Pesawaran Regency, Lampung Methods: This study was conducted in accordance with the WHO protocol for anti-malarial drug efficacy test in uncomplicated falciparum malaria. Combination therapy used artesunate-amodiaquine and primaquine. Therapeutical response and Parasite Clearance Time was observed during 28 days of observation. Diagnosis polymorphism by PCR-RFLP technique used the patient's blood (EDTA and filter paper) were taken a tH0.Results: There were 63(85.1%) patients of Adequate Clinical and Parasitological Response, Early Treatment Failure were 5 (6.8%) people, Late Clinical And Parasitological Failure 2 (2.7 %) people, and Late Parasitological Failure 4 (5.4%) people. Seven (13.5%) patients were found with N86Y pfmdr1 gene polymorphism with mixed type (mutant type and wild-type) in which 1 (9.1%) people had failed therapy and 6 (14.6%) people were cured. There was no association between pfmdr1 gene polymorphism with artesunate-amodiaquine failed therapy (RR: 0.64, 95% CI0.97-4.27, p: 1.000). Parasite Clearance Time on pfmdr1 polymorphism group was 2.14 ± 1.21 days and 1.79 ± 1.06 days in group without pfmdr1 polymorphisms. There was no significant difference between both groups (p =0.434).Conclusions:The frequency of pfmdr1 N86Y polymorphism in Puskesmas Hanura, Pesawaran Regency, Lampung was 13,5%. There was no significant difference between the rate of treatment failure patients with pfmdr1 N86Y polimorphism compared with patients without pfmdr1 N86Y polymorphism. There was no significant difference between Parasite Clearance Time patients with pfmdr1 N86Y polymorphism compared with patients without pfmdr1 N86Y polymorphism.Keywords: falciparum malaria, artesunat-amodiakuin, polymorphism, pfmdr1
Universitas Gadjah Mada
2018-10-05
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
https://jurnal.ugm.ac.id/bik/article/view/17610
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 4 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 4 (2018)
2356-3931
en
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/17672
2019-08-05T07:20:12Z
bik:ART
The influence of acetylation status of tuberculosis patients on the isoniazid serum concentrations and sputum conversion after intensive phase therapy
Anggraini, Dwi Indria
Kristin, Erna
Dwiprahasto, iwan
acetylation status; isoniazid level; success of therapy; conversion sputum; intensive phase treatment; tuberculosis
Isoniazid (INH), one of the major antituberculosis drugs, is metabolized by acetylation. Previously study proved the significant differences of serum INH concentration between subject with fast and slow acetylation status. However, the correlation of acetylation status with treatment outcome after fixed-dose combination antituberculosis therapy (FDC-ATT) was not explained. The aim of this study was to evaluate the influence of acetylation status on the treatment outcome and the serum INH concentrations in the adult tuberculosis patients underwent FDC-ATT. A cross sectional study was carried out on 31 tuberculosis patients. Acetylation status was measured by spectrophotometer and serum INH concentration was measured by high performance liquid chromatography (HPLC). Sputum conversion assay was conducted by Ziehl Nelsen method. t-Test, chi square, Mann-Whitney, and Fisherman were used to analyze the data. The proportion of the fast acetylator was 61.3%, whereas the slow acetylator was 38.7%. The proportion of success and failure sputum conversion were 83.9% and 16.1%, respectively. The mean serum INH concentration in the fast acetylator groups (1.52 ± 0.15 μg/mL) was significantly lower than that in the slow acetylator groups (3.84 ± 0.35 μg/mL). The failure conversion risk of the fast acetylator group was about two folds higher than the slow acetylator group, although it was not significantly different (RR=2.53; 95% CI=0.32-20.00; p>0.05). Moreover, the mean serum INH concentration in success (2.46 ± 0.31 μg/mL) and failure (1.89 ± 0.20 μg/mL) sputum conversion was not significantly different (p>0.05). In conclusion, the acetylation status does not influence the sputum conversion in adult tuberculosis patients after FDC-ATT although the serum INH concentration on slow acetylation status is higher than that fast acetylation status.
Universitas Gadjah Mada
2018-03-26
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/17672
10.19106/JMedSci005001201807
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 1 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 1 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/17672/pdf
Copyright (c) 2018 Dwi Indria Anggraini, Erna Kristin, iwan Dwiprahasto
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/17683
2019-05-20T04:15:52Z
bik:ART
Ethanolic extract of the Centella asiatica (L.) Urb. leaf decreases cerebellar brain-derived neurotrophic factor (BDNF) levels in rats after chronic stress
Sari, Dwi Cahyani Ratna
Juananda, Desby
Ar-Rochmah, Mawaddah
Romi, Muhammad Mansyur
Arfian, Nur
BDNF - Centella asiatica (L.) Urb. - cerebellum - chronic stress - prevention
Chronic stress produces glucocorticoid-induced neurotoxicity that may lead to alterations of the brain-derived neurotrophic factor (BDNF) concentration in the brain. Cerebellum is known to be severely affected by glucocorticoids-associated oxidative damage. Centella asiatica (L.) Urb. may protect neurons from oxidative damage. This study aimed to investigate the effect of ethanolic extract of C. asiatica (L.) Urb. leaf on the rat cerebellar BDNF levels following stress. Twenty young-adult male Sprague Dawley rats were randomly assigned into four experimental groups. The stress control group received aquadest, and the other groups were treated with different doses of the C. asiatica (L.) Urb. extract i.e 150 (CeA150), 300 (CeA300) and 600 (CeA600) mg/kg body weight/day orally, respectively and followed by chronic footshock stress for 28 days. Upon completion of the experimental period, all animals were sacrificed and the cerebellar was isolated. The BDNF levels from the cerebellar tissue lysate was measured using ELISA. The mean BDNF levels of the cerebellar tissue in the stress control, CeA150, CeA300 and CeA600 groups were 1217.10±301.40; 771.46±241.45; 757.05±268.29; and 627.00±246.02 pg/mL, respectively. Post-hoc analysis showed a significant difference between the control and treatment groups (p< 0.05). In conclusion, the ethanolic extracts of the C. asiatica (L.) Urb. leaf decrease the cerebellar BDNF levels in rats after chronic stress.
Universitas Gadjah Mada
2018-04-10
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/17683
10.19106/JMedSci005002201801
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 2 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 2 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/17683/pdf
Copyright (c) 2018 Dwi Cahyani Ratna Sari, Desby Juananda, Mawaddah Ar-Rochmah, Muhammad Mansyur Romi, Nur Arfian
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/17777
2019-08-08T06:04:08Z
bik:ART
Mechanism of cytotoxic activity of chalcone derivatives against K562 leukemia cell lines
Novilla, Arina
Mustofa, .
Astuti, Indwiani
Jumina, .
Suwito, Hery
anticancer - chalcone derivatives – leukemia – K562 cell line - PI3K/Akt signaling
Two chalcone derivatives i.e. (E)-1-(4-aminophenyl)-3-(2,3dimethoxyphenyl)-prop-2-en-1-one (Compound-1), and (E)-1-(4-aminophenyl)-3-phenylprop-2-en-1-one) (Compound-2),has been proven to have potential cytotoxic activity. The aim of this study was toevaluate the effect of these compounds on PI3K/Akt signalling pathway in K562 celllines. After incubation with the tested compounds, AKT, caspase-3, STAT3 and cyclinD1 concentrations were measured using ELISA. Furthermore, cell cycle was analysedusing flowcytometry. Imatinib and isotretinoin were used as positive control, whereascell culture without treatment was used as negative control. The AKT concentration aftertreatment with Compound-1 and -2 was significantly lower than that control, imatiniband isotretinoin (p<0.05). The apoptotic indices after treatment with Compound-1 and-2 were significantly higher than control, however they were lower than imatinib andisotretinoin (p<0.05). The caspase-3 concentration after treatment with Compound-1 at5 and 10 μg/mL and Compound-2 at 10 μg/mL was significantly higher than that controland imatinib, however it was lower than isotretinoin (p<0.05). The STAT3 concentrationafter treatment with Compound-1 and -2 was significantly lower than that control andisotretinoin at 50 μg/mL (p<0.05) and similar with imatinib (p>0.05). The cyclin D1concentration after treatment with Compound-1 and -2 was significantly lower than thatcontrol, imatinib and isotretinoin (p<0.05). In addition, Compound-1 and -2 arrested G0/G1 and G2/M phase in K562 cell lines, with comparable results to imatinib and isotretinoin.In conclusion, the mechanism of cytotoxic activity of Compound-1 and -2 are through thePI3K/Akt signalling pathway inhibition, apoptosis induction by upregulation of apoptoticmarkers, and inhibition of cell cycle progression by regulating cell cycle-related factors.
Universitas Gadjah Mada
2017-12-27
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/17777
10.19106/JMedSci004904201701
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 49, No 4 (2017); 153-164
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 49, No 4 (2017); 153-164
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/17777/pdf_1
https://jurnal.ugm.ac.id/bik/article/downloadSuppFile/17777/1061
Copyright (c) 2017 Arina Novilla, . Mustofa, Indwiani Astuti, . Jumina, Hery Suwito
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/17835
2020-06-01T06:13:40Z
bik:ART
Sinonasal or nasopharyngeal undifferentiated Carcinoma?: diagnostic pitfall and the role of Epstein-Barrvirus (EBV) and human papillomavirus(HPV) examination
Widayati, Wahyu Tri
Dwianingsih, Ery Kus
Ardianto, Bustanul
Heriyanto, Didik Setyo
Indrasari, Sagung Rai
Herdini, Camelia
Irianiwati, .
undifferentiated carcinoma; sinonasal carcinoma; nasopharyngeal carcinoma; Epstein-Barr virus; human papilloma virus
Undifferentiated carcinoma of the head and neck is frequently observed in nasopharynx, however it may also occur in oropharynx, salivary gland and sinonasal. Overlapping lesions in those regionscreate difficulty in determining the origin of the tumor. Thus, it causes diagnostic pitfall not only for pathologists, but also for clinicians. A 40 yearold man, presented with nasal obstruction, epistaxis, diplopia, and headache for a yearand showed nasal cavitysinistra and nasopharynx masses on CT-scan. Lymph node enlargement was not detected. First biopsywas performed and histopathologically diagnosed as nasopharyngeal undifferentiated carcinoma(NPC), extended into nasal cavity. Chemo-radiation protocol for NPC was conducted, and showing uncomplete response. Second biopsy was done, and reviewed with the first biopsy result. Thetumourwas arranged insolid, syncytial and trabecular pattern, with vesicular nuclei, prominent nucleoli, and lack of lymphoplasmacytic infiltrat. Immunohistochemistry (IHC) analysis of p16, EBNA1 and LMP1 were negative. PCR analysis of HPV-18 was positive, while EBV detection showed negative result. General association of EBV with NPC suggests that the presence of latent EBV infection can serve as a positive marker for NPC. Therefore, in this case, the EBV negativity and strong HPV association led to diagnosis of SNUC. The distinction of sinonasal undifferentiated carcinoma (SNUC) or from NPC was important for appropriate management and therapy.
Universitas Gadjah Mada
2020-03-25
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/17835
10.19106/JMedSci005202202008
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 52, No 2 (2020)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 52, No 2 (2020)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/17835/pdf
Copyright (c) 2020 Wahyu Tri Widayati, Ery Kus Dwianingsih, Bustanul Ardianto, Didik Setyo Heriyanto, Sagung Rai Indrasari, Camelia Herdini, . Irianiwati
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/18083
2019-08-08T05:40:18Z
bik:ART
The effects of furosemide on kidney damage in acute kidney injury rat models
Afifah, Afifah
Ngatidjan, .
Arfian, Nur
acute kidney injury - ischemic-reperfusion – furosemide – creatinine – kidney tubular injury
The most frequent cause of acute kidney injury (AKI) is ischemia reperfusion injuriesthat causes inflammation. Furosemide is still used in AKI’s therapy. The advantages anddisadvantages of furosemide in AKI remain controversial. The aim of the study was toinvestigate the effect of furosemide on kidney damage in AKI rat models. Twenty-fivemale (2-3 months old) Sprague-Dawley rats were divided into 5 groups; sham operation(SO, n=5), ischemic-reperfusion (IR, n=5), IR+furosemide 3.6 mg/kgBW (IR+F1,n=5), IR+furosemide 7.2 mg/kgBW (IR+F2, n=5), and IR+furosemide 14.4 mg/kgBW(IR+F3, n=5). Abdominal surgery was performed under ketamine anesthesia to produceischemic reperfusion (IR) by mean of renal artery clamping for 45 min. Urine output,serum creatinine level, tubular injury score, and TLR4 gene expression were examinedto investigate kidney damage. Periodic acid-schiff (PAS) staining was measured toexamine kidney tubular injury. Data were analyzed using One-Way ANOVA and Kruskal-Wallis test with significance level of p<0.05. AKI rat models which were given 3.6 and7.2 mg/kgBW of furosemide (0.014±0.001 mL/min; and 0.012±0.007) showed higher(p>0.05) creatinine clearance compared to IR (0.009±0.003) while administration of 14.4mg/kgBW furosemide (0.009±0.004) denoted equal creatinine clearance to IR (p>0,05).Kidney tubular injury score of 3.6 mg/kgBW furosemide (2.89±0.13) was lower (p>0.05)than IR (3.26±0.19) whereas 7.2 mg/kgBW and 14.4 mg/kgBW furosemide (3.55±0.26;3.83±0.19) were higher (p<0.05) than IR. Administration of 3.6 mg/kgBW furosemide(0.99±0.08) indicated lower (p<0.05) TLR4 gene expression than IR (1.20±0.08) whilst7.2 mg/kgBW furosemide (1.23±0.13) was not-significantly higher (p>0.05) and 14.4 mg/kgBW furosemide (1.63±0.12) was significantly higher (p<0.05) than IR. In conclusion,administration of 3.6 mg/kgBW furosemide reduces kidney damage in AKI rat modelswhile higher dosages (7.2 mg/kgBW and 14.4 mg/kgBW) increase kidney damage.
Universitas Gadjah Mada
2018-11-09
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/18083
10.19106/JMedScie/005003201801
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 3 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 3 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/18083/pdf
Copyright (c) 2018 Afifah Afifah, . Ngatidjan, Nur Arfian
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/18096
2019-11-14T07:32:11Z
bik:ART
An evaluation study of enzyme-linked immunosorbent assay (ELISA) using recombinant GRA1 protein for detection of igg antibodies againts Toxoplasma gondii infections
Muflikhah, Nina Difla
Artama, Wayan Tunas
Toxoplasmosis, recombinant protein, ELISA, diagnostic tools
Reliable laboratory testing is important to detect Toxoplasma gondii infection and focuses on improving the low cost and easy to use the diagnostic instrument. Enzyme-linked immunosorbent assay (ELISA) method can be used to determine a large number of samples within a short period of time which based on antibody or antigen detection. We tested the sensitivity and specificity of GRA1 protein of as antigen using the ELISA method to toxoplasmosis diagnosis and compared it with commercial ELISA kit. Seventy sera samples were collected and tested using indirect ELISA, commercial ELISA kit and GRA1 protein-coated as antigen. The results showed 48 and 51 samples had positive IgG antibody using ELISA-GRA1 and commercial ELISA kit, respectively. The GRA1 sensitivity and specificity on ELISA were 100% and 86.36%, respectively. Whereas positive predictive value (PPV) was 94.11%. This result indicated that the recombinant GRA1 protein is a highly immunogenic protein in human toxoplasmosis and marker for toxoplasmosis screening.
Universitas Gadjah Mada
2019-07-02
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/18096
10.19106/JMedSci005102201904
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 51, No 2 (2019); 128-133
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 51, No 2 (2019); 128-133
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/18096/pdf
Copyright (c) 2019 Nina Difla Muflikhah, Wayan Tunas Artama
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/18860
2018-06-19T00:09:34Z
bik:ART
Effect of Syzigium cumini (pulp) extract on malondyaldehyde and blood pressure in chronic restraint stress rat
Suryajayanti, Mega Febia
Dewi, Anggi Laksmita
Nurrahma, Bira Arumndari
Putri, Ayu Dwi Silvia
Khairia, Zunamilla
Farmawati, Arta
ABSTRACTIntroduction. Chronic stress disturbs the equilibrium of oxidant-antioxidant redox in the human body which accelerates cellular aging. This condition promotes earlier onset of age-related diseases. Human body needs exogenous antioxidant to fight stress. Syzigium cumini has high antioxidant activity because it’s rich in anthocyanin. It has potential to become natural antioxidant source thus it needs to be developed.Aim. This study aims to investigate the effect of Syzigium cumini (Pulp) extract on the stress biomarkers which are Malondialdehyde and blood pressure in rat induced by restraint stress.Method. Chronic stress phase is conducted with male Wistar rats (n=20) which were divided into 4 groups (normal control, negative control, and two treatment groups with the ethanol extract of Syzygium cumini (Pulp) at 20 mg/200 gram and 40 mg/200 gram body weight). Restraint stress was carried out by placing the animal in narrow tube for 30 minutes daily (7 days). Blood pressure was evaluated before and after the treatment while Malondialdehyde was evaluated after the treatment. The One-way ANOVA was used to analyze the parameters.Results. Based on chronic stress phase, treatment groups show significant result in preventing the increase in blood pressure (P < 0,001). The increased levels of Malondialdehyde were prevented significantly in all treatment groups (P < 0,001)Conclusion. In chronic stress phase, Syzygium cumini (Pulp) extract can become an antihypertensive agent. Syzygium cumini (Pulp) extract shows an effect in decreasing lipid peroxidation products. This result shows that the Syzygium cumini (Pulp) extract has potent antioxidant function.Keywords. Syzigium cumini, restraint stress, malondialdehyde, MDA, blood pressure
Universitas Gadjah Mada
2017-03-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/18860
10.19106/JMedSci004903201703
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 49, No 3 (2017)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 49, No 3 (2017)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/18860/pdf
Copyright (c) 2017 Mega Febia Suryajayanti, Anggi Laksmita Dewi, Bira Arumndari Nurrahma, Ayu Dwi Silvia Putri, Zunamilla Khairia, Arta Farmawati
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/18863
2019-08-08T06:04:08Z
bik:ART
R-Peaks Detection Method for Classifying Arrhythmia Disorder
Nugraha, Anggit Ferdita
Pramudita, Brahmantya Aji
Setiawan, Noor Akhmad
Nugroho, Hanung Adi
electrocardiography - R-peaks detection - QRS complex - arrhythmia disorder - Pan-Tompkins method
Electrocardiography (ECG) is a non-invasive technique that is used to diagnose heartabnormalities. ECG records all heart activities and represent them using bio electricsignals. Arrhythmia is one of the cardiac disorder that can be detected using ECG.Arrhythmia need to be detected early because of an early symptom of heart diseaseas deadly as coronary heart disease and heart failure. Arrhythmia described using thedifference between the R-peaks based on QRS complex. Therefore, R-peaks detection willbe an important factor that can be used to classify arrhythmia disease. One of the widelyused methods to detect R-peaks is Pan-Tompkins method. Pan-Tompkins method used athreshold value approach to get all location of R-peaks point from the ECG signals. Thisstudy proposed a development based on Pan-Tompkins method by change the thresholdvalue using normalize technique and moving windows approach to get all location ofR-peaks point from the ECG signals. This study uses MIT-BIH arrhythmia dataset. Thismethod can show the R-peaks detection with 99.83% sensitivity and 0.40% total errorrate detection. Hence, this method has potential to be used for classifying arrhythmiadisorder based on the R-peaks point.
Universitas Gadjah Mada
2017-12-27
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/18863
10.19106/JMedSci004904201705
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 49, No 4 (2017); 191-199
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 49, No 4 (2017); 191-199
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/18863/pdf
Copyright (c) 2017 Anggit Ferdita Nugraha, Brahmantya Aji Pramudita, Noor Akhmad Setiawan, Hanung Adi Nugroho
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/18869
2019-11-26T06:35:56Z
bik:ART
The effect of long-term high-fat diet in ovariectomized Wistar rat (Rattus norvegicus)study on lipid profile, endothelial nitric oxide synthase(eNOS) dan endhotelin-1 (ET-1) serum
Sudarsono, Tantri Analisawati
Agustiningsih, Denny
Sunarti, S
high - fat diet - ovacriectomy - lipid profile - endothelial nitric oxide synthase (eNOS) -endothelin-1 (ET-1)
Accumulation of cholesterol in the blood will cause stiffness in arteries and trigger the formation of atherosclerotic lesions. Estrogen has a role as an antioxidant that can prevent the low density lipoprotein(LDL) oxidation. In menopause with high-fat diet, the decrease of estrogen levels will trigger cholesterol accumulation in the blood lead to endothelial dysfunction mediated by endotelin-1 (ET-1) and nitric oxide synthase (eNOS). This study aimed to investigate the effect of long-term high-fat diet on the lipid profile, serum eNOS and ET-1levels on ovariectomized rat. It was experimental using 28 female Wistar rat divided into 4 groups. Group 1 was ovariectomized mice and given a standard diet (OVX-SD), Group 2 was ovariectomized mice and given a high-fat diet (OVX-HFD), Group 3 was not ovariectomized mice and given a standard diet(SHAM-SD) group, and Group 4 was not ovariectomized mice and given a high-fat diet (SHAM-HFD).Lipid profile of blood samples was measured pre- and post-treatment, whereas serum eNOS and ET-1 levels were measured post-treatment using ELISA method. No significantly difference of lipid profileon OVX-HFD group compared to that OVX-SD was observed. The serum eNOSlevel on OVX-HFD(702.11±68.73 pg/mL) was significantly lower than that OVX-SD (857.18±118.08 pg/mL) (p<0.05). However, there was no significantly different of serum ET-1 levelbetween OVX-HFD group (299.14±146.61 pg/mL) compared to that OVX-SD (194.25±102.96 pg/mL) (p>0.05). In conclusion, the serum eNOS levelon ovariectomized rat with long-term high-fat diet is lower than that on ovariectomized rat with standard diet.
Universitas Gadjah Mada
2019-08-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/18869
10.19106/JMedSci005103201902
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 51, No 3 (2019); 195-202
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 51, No 3 (2019); 195-202
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/18869/pdf
Copyright (c) 2019 Tantri Analisawati Sudarsono, Denny Agustiningsih, S Sunarti
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/18877
2019-08-08T05:40:18Z
bik:ART
Comparison of corneal endothelial cell count and intraocular pressure in pure-dispersive and dispersive-cohesive viscoelastic protection in phacoemulsification surgery
Nugroho, Agung
Yudono, R Haryo
phacoemulsification - corneal endothelial cell - intraocular pressure – viscoelastic – pure-dispersive - dispersive-cohesive
There are so many aspects should be regarded when use viscoelastic device during phacoemulsification surgery. The advantages and disadvantages of pure-dispersive viscoelastic and dispersive-cohesive viscoelastic always require more our attention to use it conveniently. The purpose of the study was to compare between pure-dispersive viscoelastic versus dispersive-cohesive viscoelastic in phacoemulsification surgery in that of corneal endothelial cell count and intraocular pressure (IOP) change. This was a cross-sectional study involving 41 eligible patients who underwent phacoemulsification surgery by single operator. Data including characteristics of cataract patients, corneal endothelial cell count and IOP were taken before and after surgery. Data of characteristics of cataract patients were reported descriptively and compared using Anova and t-test. The mean change in corneal endothelial cell count on pure-dispersive viscoelastic group (71.99±71.20 cells/mm²) was lower than that on the dispersive-cohesive viscoelastic group (117.62±78.29 cells/mm²). However, it was not significantly different. The mean change in IOP on pure-dispersive viscoelastic group (0.75±1.626 mmHg) was significantly lower than that on dispersive-cohesive viscoelastic group (1.90±0.995 mmHg) (p=0.000). In conclusion, the increase of IOP in dispersive-cohesive viscoelastic group is higher than that on pure-dispersive viscoelastic group. However, there is no significant difference of the mean change in corneal endothelial cell on the both groups.
Universitas Gadjah Mada
2019-01-28
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/18877
10.19106/JMedScie/0050032018013
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 3 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 3 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/18877/pdf
Copyright (c) 2019 Agung Nugroho, R Haryo Yudono
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/18950
2018-07-23T03:30:51Z
bik:ART
Cinnamomum burmannii improves insulin serum level in the normal obese subjects : preliminary study
Hendarto, Hari
Sari, Flori R
Adhyanto, Chris
Cinnamomum burmannii – obesity – insulin - body mass index - lipid profile
Obesity is characterized with excessive accumulation of the body fat which occurs whenthe energy intake exceeds the expenditure. It is routinely associated with insulin resistanceand hyperinsulinemia. Additionally, suppressing insulin level protects female mice fromweight gaining. Cinnamon [Cinnamomum burmannii (Ness) Bl. Cortex] suppresseshyperinsulinemia condition in the type 2 diabetic rat suggesting the possible beneficial itsrole in the obesity. We aimed to investigate the effect of Cinnamon extract in the normalobese subjects. In this preliminary cross-over clinical trial, 24 normal obese subjectswere recruited and divided randomly into two groups i.e. treatment and placebo. Twograms of the cinnamon extract were given twice daily for 56 days in the treatment group.Normal obese subjects given placebo were allocated as the placebo group. After thetreatment, each of the group ran a one month run-in period, then the groups were crossoveredfor the next 56 days. Body mass index (BMI), insulin serum level, cholesterol andtriglyceride plasma levels were measured at the beginning and at the end of the study.No diet restriction nor exercise intervention was given during the study. At the end of thestudy, BMI in the treatment group (58%) were slightly reduced when compared to theplacebo group (33%), however, it was not significantly different (p>0.05). Moreover,significantly reduction in the insulin serum level was observed in 63% subject in thetreatment group compared to 33% subject in the placebo group (p < 0.05). Additionally,there were no significant differences of cholesterol and triglyceride plasma level observedin the both group. In conclusion, cinnamon extract may give beneficial role in the normalobese subjects by suppressing the serum insulin level. Further studies are required toelucidate the specific role of cinnamon in preventing weight gain.
Universitas Gadjah Mada
2018-03-26
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/18950
10.19106/JMedSci005001201808
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 1 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 1 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/18950/pdf
Copyright (c) 2018 Hari Hendarto, Flori R Sari, Chris Adhyanto
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/18951
2018-04-09T08:47:56Z
bik:ART
The Influence of Long-term Diabetes Mellitus on Pain Response in Mice: In Vivo Models of Painful Diabetic Neuropathy (PDN)
Fajrin, Fifteen Aprila
Susilowati, R
Nurrochmad, A
Nugroho, AE
ABSTRACTPainful diabetic neuropathy (PDN) is a complication of long-term Diabetes Mellitus (DM) characterized by hyperalgesia and allodynia. In streptozotocin (STZ)-induced diabetic mice, higher dose of STZ and lengthen hiperglycemic condition results in better model of PDN. However, higher dose of STZ tend to induce mortality. Evaluate the doses of STZ that caused PDN with less mortality rate and the timing of pain behavior development in mice model of PDN. Balb/c mice were divided into non-diabetic and STZ-induced diabetic group. The doses of STZ were started from 180 mg/kg i.p. Serum glucose levels were measured 7 days after induction. Mice with glucose levels ≥ 200 mg/dl were considered as diabetic. Pain behaviour was determined by four method i.e. hot plate, tail flick test, von Frey fillament and Randall Selitto,measured on week-0 (baseline), 1, 2, 3, 4 and 5. Data were presented as mean±SEM. The mean differences between weeks were evaluated by One-Way ANOVA and the mean differences between two groups by independent t-test. STZ doses 180 mg/kg, 150 mg/kg and 120 mg/kg caused 100% death and STZ 90 mg/kg failed to induce diabetic condition. STZ 110 mg/kg resulted in 0% mortality while it induced diabetes in 100% mice. Latency time toward thermal stimulus decreased to 5.8 s at 1st week after the mice become diabetes (p<0.05) and it was continued decrease until 4th week. The same result was also showed in tail flick test and Randal Selitto. The pain sensitivity determined by von Frey filament decreased to 1.37 g at week 1 (p<0.05) and continued decrease until 5th week. Optimum dose of STZ to induce PDN was 110 mg/kg. Pain behaviour of diabetic group was observed at 1st week after diabetic and continued until 4th week.Keywords: PDN, hot plate, tail flick test, von Frey fillament, Randall Selitto
Universitas Gadjah Mada
2017-03-03
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/18951
10.19106/JMedSci004903201701
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 49, No 3 (2017)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 49, No 3 (2017)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/18951/pdf
Copyright (c) 2017 Fifteen Aprila Fajrin, R Susilowati, A Nurrochmad, AE Nugroho
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/18954
2019-08-08T05:40:18Z
bik:ART
Accuracy of albumin creatinine ratio in comparison with albumine excretion rate for diagnosis diabetic nephropathy in type 2 diabetes mellitus
Fatrinawati, .
Windarwati, .
Sianipar, Osman
diabetic nephropathy - albumin creatinine ratio (ACR) - albumin excretion rate (AER) – sensitivity - diagnostic test
Diabetic nephropathy (DN) is one of complications in diabetic patients manifested bymicroalbuminuria with minimal level of 30 mg/24 hour which is measured at least 2 timesin the period of 3 to 6 months. Microalbuminuria can be measured either albumin excretion rate (AER) or albumin creatinine ratio (ACR). Measurement of ACR is an alternative parameter recommended by WHO in 2011 to diagnose diabetic nephropathy since it is more convenient, fast and not requires special preparation. The purpose of this study was to investigate accuracy of ACR to diagnose DN in type 2 diabetes mellitus (T2DM) patients.This was a diagnostic test study involving 80 T2DM patients. In this study ACR value equal or more than 30 mg/g was independently and blindly compared with AER as the gold standard. The data were analyzed using 2x2 tables in order to calculate sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV). Other data were analyzed using statistic descriptive. Eighty T2DM patients consisting of 38males and 42 females participated in this study. They had suffered from T2DM on average9.5 years, and the average of ACR value was 55.5 mg/g. Total result of true positive andtrue negative was 77. Three result were false negative but none of false positive result.The ACR value equal or more than 30 mg/g had sensitivity, specificity, PPV, and NPV of95.9%, 100%, 100%, and 66.7% respectively. In conclusion, the ACR value equal or morethan 30 mg/g derived from morning urine sample can be used to diagnose DN in T2DMpatients.
Universitas Gadjah Mada
2017-03-03
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/18954
10.19106/JMedScie/005003201806
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 3 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 3 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/18954/pdf
Copyright (c) 2017 . Fatrinawati, . Windarwati, Osman Sianipar
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/18955
2018-06-25T02:52:01Z
bik:ART
Prevalence of metabolic syndrome and its components based on International Diabetes Federation (IDF) definition in Yogyakarta Special Region, Indonesia
Jian, Choo Hao
Dewi, Fatwa Sari Tetra
Herningtyas, Elizabeth Henny
Prevalence of Metabolic Syndrome, central obesity, dyslipidemia, hypertension, Yogyakarta-Indonesia, IDF criteria
ABSTRACTMetabolic syndrome (MetS) is a group of risk factors which increase morbidity and mortality for cardiovascular disease and diabetes. The prevalence of MetS has been on the rise. No previous study has described the prevalence of MetS in Yogyakarta Special Region and its components. The study aim was to determine the prevalence of MetS and it’s components in Yogyakarta Special Region, Indonesia. A total of 766 male and female subjects aged ≥40 were analyzed in this retrospective study based on secondary data from the Indonesian Family Life Survey batch 4 (IFLS 4). MetS was defined by International Diabetes Federation (IDF) criteria with ethnicity-specific values for waist circumference. Prevalence of MetS and characteristic of each component of MetS were expressed as mean or %. The difference of the MetS components was evaluated by t-test and chi-square. Prevalence of MetS in Yogyakarta Special Region was 13.19%. The most common of MetS component was hypertension (60.44%), followed by dyslipidemia (56.27%), central obesity (32.38%), pro-inflammatory state (15.71%) and insulin resistance (0.78%). There was a higher prevalence of MetS in females compared to males (15.88 vs 10.19%), pre-elderly compared to the elderly (13.90 vs 12.19%), Javanese compared to other ethnicities (13.23 vs 10.00%), and urban compared to rural populations (15.06 vs 8.37%). In conclusion,the prevalence of MetS in Yogyakarta Special Region is 13.19% with hypertension and dyslipidemia as the most common component.
Universitas Gadjah Mada
2017-03-03
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/18955
10.19106/JMedSci004903201705
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 49, No 3 (2017)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 49, No 3 (2017)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/18955/pdf
Copyright (c) 2017 Choo Hao Jian, Fatwa Sari Tetra Dewi, Elizabeth Henny Herningtyas
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/18959
2019-11-14T07:32:11Z
bik:ART
Prevalence ratio of free fatty acid in obese group with non-alcoholic fatty liver disease
Handayani, Rosmeri
Purnamaningsih, Siti Muchayat
Sukorini, Usi
NAFLD, FFA, prevalence ratio, obese, women
Non-alcoholic fatty liver disease (NAFLD) is liver disorders characterized by macrovesicular fatty liver, fibrosis, cirrhosis that not associated with alcohol consumption. The prevalence of NAFLD has risen with a pandemic of obesity. The increase of free fatty acid (FFA) oxidation will induce endoplasmic reticulum stress that cause mitochondrial dysfunction and lead to increase reactive oxygen species (ROS) production causing apoptosis of liver cells. The aim of study was to determine the prevalence of FFA in the obese group. This was an observational analytical study with cross-sectional design to determine the prevalence ratio of FFA in the obese group with NAFLD compared to the group without NAFLD. Obese women who fulfill the inclusion and exclusion criteria were involved in this study. Five mL venous blood sample was collected for the measurement of lipid profile, liver enzyme and FFA. Fatty liver was evaluated using abdominal USG. The Chi-square test was used to analyze different proportions of FFA between the both groups. Sixty four subjects were participated in this study and classified into obese with NAFLD (39 subjects) and obese without NAFLD (25 subjects). The prevalence ratio of FFA with cutoff value ≥2.66 nmol/mL in the obese group with NAFLD was 4.3 times higher than those without NAFLD (95% IC: 3.5 – 42.3; p<0.001).
Universitas Gadjah Mada
2017-03-06
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/18959
10.19106/JMedSci005102201906
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 51, No 2 (2019); 145-151
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 51, No 2 (2019); 145-151
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/18959/pdf
Copyright (c) 2017 Rosmeri Handayani, Siti Muchayat Purnamaningsih, Usi Sukorini
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/19080
2019-10-31T02:47:45Z
bik:ART
SCN1A exon 26 variants in epilepsy and migraine patients
Lestari, Nova Dian
Mutiawati, Endang
Sadewa, Ahmad Hamim
Sjahrir, Hasan
Syahrul, .
Emril, Dessy Rakhmawati
Harapan, .
epilepsy - gene variation - migraine - mutation - SCN1A
Epilepsy and migraine are common neurological diseases in many populations. Mutation of the voltage gated natrium channel Nav1.1 (SCN1A) are important causes of different genetic epilepsies and can also cause familial hemiplegic migraine (FHM-III). This study aimed to identify SCN1A gene variation in patients with epilepsy and common migraine. Gene variation analysis of exon 26 of the SCN1A gene was carried out in 33 patients with epilepsy, 33 patients with migraine and 30 control individuals from Neurology Polyclinic at Dr. Zainoel Abidin General Hospital, Banda Aceh. The PCR and direct sequencing methods were performed in this study. SCN1A gene variations were identified in two epilepsy patients. These gene variations located at exon 26 were four silent mutations in patient E27 at position A4440T (Leu1480Leu), T4443C (Leu1481Leu), A5046G (Leu1682Leu) and C5121T (Asp1707Asp). One silent mutation in patient E30 at position G5505A (Glu1835Glu). None of these gene variations were identified in controls and patients with common migraine in this study. This study has identified 5 genetic variations of SCN1A in patients with epilepsy but not in common migraine. The mechanism and relationship between these variants and epilepsy need to be clarified.
Universitas Gadjah Mada
2017-03-06
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/19080
10.19106/JMedScie/005004201806
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 4 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 4 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/19080/pdf
Copyright (c) 2017 Nova Dian Lestari, Endang Mutiawati, Ahmad Hamim Sadewa, Hasan Sjahrir, . Syahrul, Dessy Rakhmawati Emril, . Harapan
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/19081
2019-11-27T01:26:21Z
bik:ART
The accuracy of fine needle aspiration biopsy to diagnose breast neoplasm
Felisha, Hifdza Faza
Rinonce, Hanggoro Tri
Anwar, Sumadi Lukman
Dwianingsih, Ery Kus
FNAB; breast lump; sensitivity; specificity; diagnosis
Breast lump is a very common complaint among women, especially during the reproductive year. Fine needle aspiration biopsy (FNAB) is a less invasive procedure. It is usually performed as an initial diagnosis prior to the operative procedure. The accuracy of the FNAB in Indonesia needs to be elaborated. The study aimed to evaluate the sensitivity and specificity of FNAB in diagnosing breast neoplasm. This is a retrospective study with cross sectional design, involving 145 patients with breast lump who underwent FNAB and histopathology examination in Dr. Sardjito General Hospital, Yogyakarta, from 2012 to 2014. Data analysis showed that female to male ratio was 23. 2:1 commonly occurred at 41-50 years old. Forty-one cases (28.28%) diagnosed as a benign lesion with fibrocystic changes as the most frequentcase (11.19%). The malignant case was 104 cases (71.72%) with ductal carcinoma as the highest case (51.49%). FNAB achieved a sensitivity of 85.58%, a specificity of 100% and a total accuracy of 89.66% in determining the benign or malignant breast lump. The accuracy, sensitivity and specificity of FNAB in diagnosing ductal carcinoma were 83.58%, 85.51% and 81.54%, respectively. The accuracy, sensitivity and specificity of FNAB to diagnose fibrocystic changes lesion were 85.82%, 26.67% and 93.28%, respectively. FNAB can be used as an alternative diagnostic tool to diagnose breast neoplasm. It provides rapid, cheaper, effective, valuable, and less invasive procedure in diagnosis of breast lump.
Universitas Gadjah Mada
2017-03-06
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/19081
10.19106/JMedSci005103201907
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 51, No 3 (2019); 237-245
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 51, No 3 (2019); 237-245
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/19081/pdf
Copyright (c) 2017 Hifdza Faza Felisha, Hanggoro Tri Rinonce, Sumadi Lukman Anwar, Ery Kus Dwianingsih
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/19160
2017-10-31T06:40:32Z
bik:ART
Over- and down-expression mir-29c and mir-21 after chemotherapy and radio-therapy in nasopharyngeal carcinomas and the down-regulating proteins encoding eipstein barr virus and c-Myc.
wardana, Tirta
Herawati, Cita
Oktriani, Risky
Lukman Anwar, Sumadi
Astuti, Indwiani
Aryandono, Teguh
Haryana, Sofia Mubarika
Nasopharyngeal carcinoma (NPC) is the type of cancer related to multiple risk factors, including infection by Epstein Barr Virus (EBV). Standard treatment of NPC involves radiotherapy and chemotherapy in local and advanced tumors, while metastatic cases are treated with systemic chemotherapy. However, there is limited data on the causes of tumor recurrence, resistance, and progression. Moreover, the initial symptoms of NPC were often neglected until later enlarged, thus making it difficult to manage. MicroRNA (miRNA) is short molecule with 18-24 nucleotides and functions as protein-expression regulator protein in post-transcription. This study was aimed to determine miRNA expression and its relationship with the incidence of NPC. miR-21 and miR-29c were known to be involved in the development of NPC and resistance. A total of 51 plasma samples and 17 tissue samples were collected from Dharmais Hospital. The samples were taken from 17 untreated patients, 17 treated patients, and 17 healthy participants as control. We examined miRNA, protein of protein EBV (EBNA), and c-Myc expression using immunohistochemistry and quantitative polymerase chain reaction (qPCR). Our study revealed an increased expression of miR-21 and decreased expression of miR-29c in patients with NPC. There was also a correlation between the regulation of expression of miR-21 and c-Myc in the treated group of patients, and decreased expression in patients with complete response (CR) (4.13 ± 3.65: 2.74 ± 3.23; p <0.1). The parameters tend to increase in patients with partial response (PR) (3.00 ± 5, 86 compared to 8.77 ± 8.43; p <0.5), while no significant difference in expression of miR-29c in patients with CR and PR was detected. We concluded that miRNA might be detected in the plasma of NPC patients, and miR-21 might become a useful biomarker to determine therapeutic outcome in NPC patients.Keywords: nasopharyngeal cancer; miRNA; biomarker
Universitas Gadjah Mada
2017-02-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/19160
10.19106/JMedScieSup004804201622
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 48, No 4 (2016): SUPPLEMENT
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/19160/pdf
Copyright (c) 2017 Tirta wardana, Cita Herawati, Risky Oktriani, Sumadi Lukman Anwar, Indwiani Astuti, Teguh Aryandono, Sofia Mubarika Haryana
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/19204
2019-05-21T07:25:54Z
bik:ART
Systematic Review: Pre-Stroke Use of Angiotensin Receptor Blockers and Stroke Outcomes
Dewi, Ivana Purnama
Kusmanto, Virandra B.
Dewi, Kristin Purnama
Pinzon, Rizaldy
ARB, pre-stroke, benefit, prognosis
Background: Hypertension is the major risk factor and the most important modifiable risk factor for stroke. Angiotensin Receptor Blockers (ARB) are widely used in patient at high risk of cardiocerebrovascular events. The objective of this literature review was to determine the efficacy of pre-stroke use of ARB on stroke outcomes.Methods: Major medical databases (PubMed, MEDLINE, Clinical Key, Cochrane Library, EMBASE) were systematically searched using keyword: “hypertension”, “ARB”, “stroke”, and “outcome”. The search were limited to clinical trials published within the last 10 years, written in English, with full-text availability. We used GRADE Working Group to measure the quality of evidence.Results: Four clinical studies, three retrospective studies and one nationwide population-based cohort study met our inclusion criteria with total of 102.644 patients for analysis. The scientific quality of the studies varied from poor (1 study), moderate (1 study), and high quality (2 studies). Generally, the subjects of the studies were acute ischemic stroke patients. Three studies showed pre-stroke use of ARB were significantly associated with better stroke outcomes. Only one study found different result whereas pre-stroke use of ARB did not appear to affect stroke outcomes. Outcome of the studies was explored according to morbidity (severity and functional status upon discharge) and mortality (30-days mortality or in-hospital mortality). Several limitations were present, including non-random treatment assignment, retrospective study design, and lack of data for longitudinal medication exposure in observational studies.Conclusions: This systematic review shows evidence that there is possible benefit of pre-stroke ARB treatment in relation to better ischemic stroke outcomes. However, further studies with better research method quality are still needed. The efficacy of ARB treatment in relation to other type of stroke outcomes also needs to be furtherly examined.Keywords: ARB, pre-stroke, benefit, prognosis Makalah ini dipresentasikan dalam Poster Session 11th Scientific Meeting of Indonesian Society of Hypertension, 24-26 February 2017
Universitas Gadjah Mada
Faculty of Medicine, Duta Wacana Christian University
2018-12-26
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/19204
10.19106/JMedSci005002201815
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 2 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 2 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/19204/pdf
Copyright (c) 2018 Ivana Purnama Dewi, Virandra B. Kusmanto, Kristin Purnama Dewi, Rizaldy Pinzon
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/19380
2019-11-14T07:32:11Z
bik:ART
Solitary dermal cylindroma: a rare case report
Widayati, Wahyu Tri
Irianiwati, .
dermal cylindroma – solitary – PAS - p63 - CD1a
Dermal cylindromas are rare benign skin appendageal tumors that has two clinical presentation, solitary and multiple form. The diagnosis is unusual.However, it must be considered in clinical practice.A 51-year-old woman hadpainless nodule on right arm. Skin examinatipn revealedtwo fragmented nodules, 1x0.5x0.5cm, tan to white, and rubbery. Histopathological findings revealed a well demarcated and an unencapsulated epithelial dermal tumor, composed of numerous oval and polygonal nests molded into a "jig-saw" or "mosaic" appearance at low power. The nests of cells were composed of basaloid cells with scant cytoplasm and hyperchromatic nuclei and paler cells at the center. Nests of epithelial cells were surrounded and penetrated by a thickened band of basement membrane material that was PAS-positive. Immunohistochemistry (IHC) staining with p63 showed positive staining on basaloid cells. Langerhans dendritic cells in the surrounding nests stained positive with CD1a. Solitary dermal cylindroma occurred sporadically in patients without family history of cutaneous cylindromas.Combination of morphologic, histochemystry and immunohistochemystry staining were needed for accurate diagnosis.
Universitas Gadjah Mada
2019-07-02
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/19380
10.19106/JMedSci005102201910
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 51, No 2 (2019); 181-187
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 51, No 2 (2019); 181-187
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/19380/pdf
Copyright (c) 2019 Wahyu Tri Widayati, . Irianiwati
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/22626
2019-08-05T07:20:12Z
bik:ART
Comparison of Bcl-xL protein expression in placental trophoblast cells between pregnancy complicated by severe preeclampsia and normotensive pregnancy
Hadiati, Diah Rumekti
Palupi, Arsi
Hakimi, Mohammad
Haryana, Sofia Mubarika
trophoblast; severe preeclampsia; Bcl-xL protein; apoptosis
Preeclampsia is one of the main causes of maternal and perinatal mortality and morbidity.The pathogenesis of preeclampsia remains unclear until now. It is believed thatregulation of apoptosis in trophoblast cells plays an important role in the pathophysiologyof preeclampsia. Failure of spiral arteries remodeling will eventually lead to placentalhypoxia lead to excessive trophoblast apoptosis. The molecular mechanism of apoptosisis very complicated involving many signaling molecules included Bcl-2 proteins. The Bcl-2 protein group consists of proapoptosis proteins (Bax) and apoptosis inhibitor proteins(Bcl-2 and Bcl-xL). The aimed of this stuty was to compare the expression of Bcl-xLprotein in placental trophoblast cells of pregnancy complicated by severe preeclampsiawith that normotensive pregnancy. This study was an observational study with crosssectional design involving 43 pregnancy patients with severe preeclampsia and 38normotensive pregnancy who treated in Dr. Sardjito General Hospital, Yogyakarta fromOctober 2011 until March 2012. Placenta samples were obtained from all subjects forBcl-xL protein expression analysis using immunohistochemistry technique. Data wereanalyzed using independent t-test, chi-square test, and logistic regression. A p value<0.05 was considered significant. Significant difference in Bcl-xL protein expressionin trophoblast cells of pregnancy complicated by severe preeclampsia (1.29 ± 0.12)compared to that normotensive pregnancy (1.71 ± 0.14) was reported (p = 0.00). Inaddition, logistic regression test showed that diagnosis of severe preeclampsia had astatistically significant role in Bcl-xL protein expression (p= 0.000). In conclusion, theexpression of Bcl-xL protein is lower in pregnancy complicated by severe preeclampsiacompared to normotensive pregnancy.
Universitas Gadjah Mada
2018-03-26
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/22626
10.19106/JMedSci005001201804
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 1 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 1 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/22626/pdf
Copyright (c) 2018 Diah Rumekti Hadiati, Arsi Palupi, Mohammad Hakimi, Sofia Mubarika Haryana
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/23884
2019-08-08T05:40:18Z
bik:ART
Precocious puberty in McCune-Albright syndrome: a case report
Lestari, Rianti Puji
Sutomo, Retno
Julia, Madarina
McCune-Albright syndrome - precocious puberty – tamoxifen – genetic disorders - gonadotropins
McCune-Albright syndrome (MAS) is a rare disease characterized by a triad of fibrousdysplasia, cafe-au-lait spots and peripheral precocious puberty. We reported a 5-year-8-month old girl with MAS who has been followed-up for 2 years and 8 months. Shewas referred to pediatric endocrinology clinic in our hospital for vaginal bleeding at ageof 2 years 11 months. She had peripheral precocious puberty, i.e. increased estrogenlevel associated with very low gonadotropins, and cafe-au-lait spots on her face and wasdiagnosed as MAS. The patient was treated with estrogen receptor blocker (tamoxifen).She had no menses during the 2 years and 8 months of tamoxifen treatment. Her growthrate and bone maturation were also in normal ranges. However, at the end of tamoxifentreatment she had an episode of vaginal bleeding so that we had to change to othertreatment modalities.
Universitas Gadjah Mada
2018-12-26
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/23884
10.19106/JMedScie/0050032018014
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 3 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 3 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/23884/pdf
Copyright (c) 2018 Rianti Puji Lestari, Retno Sutomo, Madarina Julia
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/24710
2019-08-08T06:04:08Z
bik:ART
Development of motor learning implementation for ischemic stroke: finding expert consensus
Rahayu, Umi Budi
Wibowo, Samekto
Setyopranoto, Ismail
model development - application of motor learning - ischemic stroke - expert consensus - neurorestoration
The main purpose of this study was to investigate motor learning implementation forischemic stroke from experts on the field of motor learning in stroke patients includingthe neurologist, medical rehabilitation specialists and physiotherapists. To collect thedata and answer the research questions, statements were made on the basis of thestudy of literature and the grains exploration of the statements in the questionnaire usingthe Delphi Method. Formulation development model was based iteration or judgmentof experts. Validation assessment statement grain tested by the Content Validity Ratio(CVR) and Content Validity Index (CVI) was used to analyze the data. The finding clearup that CVR value of each item statement was 1 and the value of CVI also 1. There were6 indicators in a 26-point declaration on the implementation of the development model ofmotor learning intervention for ischemic stroke. Six indicators included basic theories thatsupport the importance of intervention motor learning, motor learning stages, principlesof motor learning, dosage, timing of and kinds of motor learning interventions that can beprovided as well as application development intervention model motor learning, allowinggiven for ischemic stroke. In conclusion, based on the content validity of the results ofthe consensus expert judgments are six indicators of the importance of motor learningapplication for ischemic stroke.
Universitas Gadjah Mada
2017-12-27
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/24710
10.19106/JMedSci004904201706
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 49, No 4 (2017); 200-216
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 49, No 4 (2017); 200-216
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/24710/pdf
Copyright (c) 2017 Umi Budi Rahayu, Samekto Wibowo, Ismail Setyopranoto
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/24920
2019-08-05T07:20:12Z
bik:ART
Insecticide resistance and mechanisms of aedes aegypti (Diptera: Culicidae) in Yogyakarta, Indonesia
Mulyaningsih, Budi
Umniyati, Sitti Rahmah
Satoto, Tri Baskoro Tunggul
Diptyanusa, Ajib
Nugrahaningsih, Dwi Aris Agung
Selian, Yahiddin
Organophosphate; pyrethroid; biological assay; biochemical assay; molecular assay
For several decades, applications of organophosphates and pyrethroids insecticides have been extensively used to control Aedes aegypti as the primary dengue vector. Hence it is important to study dengue vector resistance status and its mechanisms in relation to long term use of insecticides. This study aimed to determine the resistance status and to characterize mechanisms of Ae. aegypti to organophosphates and pyrethroids using biological, biochemical and molecular assays. Larvae and puppae of Ae. aegypti were collected in the field of Plosokuning, Minomartani, Sleman, Yogyakarta, Indonesia. The biological assay was carried out using CDC Bottle Bioassay to test the resistant status to malation and cypermetrin. The biochemical assay was conducted using microplate assay with substrate α-naphthyl acetate to test the presence of esterase elevated activity, and the molecular assay was done using PCR with AaSCF7 and AaSCR7 primer to detect of point mutation at 1534 site, that located in the area of segment 6 of domain III. The biological assay showed Ae. aegypti suggests the possibility of resistance to malathion (mortality 82%) that needs to be confirmed further and already resistant to cypermetrin (mortality 76%). The biochemical assay of Ae. aegypti showed the presence of non-specific esterase elevated activity. The PCR method showed specific DNA bands were formed with the size of 748bp, and with sequencing showed there has been F1354C point mutation of voltage gated sodium chanel gene in the area of segment 6 of domain III. Long term use of insecticides did not successfully eliminate the targeted dengue vector, because Ae. aegypti mosquitoes were resistant to both insecticides. The results demonstrate the importance of designing better health policies regarding insecticide usage
Universitas Gadjah Mada
2018-03-26
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/24920
10.19106/JMedSci005001201803
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 1 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 1 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/24920/pdf
Copyright (c) 2018 Budi Mulyaningsih, Sitti Rahmah Umniyati, Tri Baskoro Tunggul Satoto, Ajib Diptyanusa, Dwi Aris Agung Nugrahaningsih, Yahiddin Selian
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/25465
2020-06-01T06:13:40Z
bik:ART
The prognostic value of lymph nodes mRNA CXCL12 expression in the breast cancer
Safitri, Idha
Widodo, Irianiwati
Heriyanto, Didik Setyo
breast carcinoma; CXCL-12 mRNA expression; lymph nodes; metastasis; prognostic factor;
Prognosis of breast carcinoma is influenced by age, tumor size, histological grade and type, lymph node status, as well as metastasic status. Chemokine receptor CXCR-4 with its ligand, CXCL-12, may play an important role in metastasis of breast carcinoma. However, the role of CXCL-12 mRNA as a prognostic factor and a therapeutic target of human breast cancer remains controversial. This study aimed to investigate the level of CXCL-12 mRNA expression in lymph nodes of patients with invasive ductal breast carcinoma and the difference within the prognostic factors. Axillary lymph nodes obtained from 50 cases of invasive ductal breast carcinoma, were divided into two groups, with and without lymph node metastasis. Each group consisted of 25 cases. Total RNA was extracted from formalin-fixed paraffin-embedded. The CXCL-12 mRNA expression was examined using qRT-PCR method. The mean differences between the two groups were analyzed using Mann-Whitney test. The differences between CXCL-12 mRNA expression and each prognostic factor ware analyzed using Mann-Whitney comparison test. CXCL-12 mRNA expression was significantly higher in the lymph node of patients with metastasis of breast carcinoma compared to the non-metastasis cases (p<0.01). There were significant differences between CXCL-12 mRNA expression with poorly histological grade (p=0.003), bigger primary tumor size (p=0.005) and age of ≥45 y.o (p=0.012) in the metastatic group, but there were no significant differences between both age of <45 and ≥45 y.o.This study suggests that the higher CXCL-12 mRNA expression level are associated with bigger tumor size and poor differentiation in breast cancer patient with lymph nodes metastasis
Universitas Gadjah Mada
2019-10-04
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/25465
10.19106/JMedSci005202202002
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 52, No 2 (2020)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 52, No 2 (2020)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/25465/pdf
Copyright (c) 2019 idha safitri, Irianiwati Widodo, Didik Setyo Heriyanto
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/25763
2019-08-08T05:40:18Z
bik:ART
Factors influencing plateletpheresis donations in Yogyakarta, Indonesia
Wicaksana, Aditya
Triyono, Teguh
Intansari, Umi
platelet – plateletpheresis – pre-donation – post-donation - platelet count- product
Despite its life saving potential, regrettably, blood transfusion has yet to be optimally applied in Indonesia. Funding difficulties and both public and professional lack of knowledge hinders its progress. More lives can be saved by using a safer, more proper, and specialized blood transfusion procedure. Plateletpheresis, one method to obtain platelet products, requires a different donation procedure than that of whole blood-derived platelet. High quality plateletpheresis product donation will positively impact the transfusion efficacy and platelet recovery of the recipient, improving patient’s clinical state. This study analyzes factors from both the donor and donation procedure that influence the quality of plateletpheresis product. The study analyzes data of plateletpheresis donations from blood transfusion service and plateletpheresis transfusions from medical records at Dr. Sardjito General Hospital, Yogyakarta, Indonesia, within the period of August 2012 to January 2013 using cross sectional design. Forty-four plateletpheresis donations were obtained during the study. All donors were male with the following mean values; age 31.9 ± 9.9 years, weight 70.2 ± 10.2 kg, body mass index (BMI) 24.7 ± 3.2 kg/m2, hematocrit 44 ± 3.2 %, and procedure time 84.2 ± 19.2 min. The median value of platelet yield was 3.2x1011 (2.1x1011 – 4x1011). The median value of pre-donation platelet count was 248.5x103/μL (204x103/μL – 391x103/μL) and the mean value of product volume was 275 ± 22.9 mL. The results showed that pre-donation platelet count (r = 0.329; p < 0.05) and product volume (r = 0.661; p < 0.05) positively correlated to the yield of platetetpheresis products. However, the yield of plateletpheresis products was not correlated to the post-transfusion platelet count (r = 0.327; p > 0.05). In conclusion, pre-donation platelet count and product volume of plateletpheresis influence the yield of plateletpheresis. However, the yield is not correlated to the post-donation platelet count. Thus, other clinical factors should be considered
Universitas Gadjah Mada
2018-10-05
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/25763
10.19106/JMedScie/005003201809
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 3 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 3 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/25763/pdf
Copyright (c) 2018 Aditya Wicaksana, Teguh Triyono, Umi Intansari
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/25959
2019-08-08T05:40:18Z
bik:ART
Peppermint oil prevented oxidative stress in experimental animal – induced acute single bout of eccentric exercise (ASBEE): study on blood catalase and hydrogen peroxide (H2O2) and glucose transporter-4 (GLUT-4) expression on the muscle cells
Aryanti, Dewi
Agustiningsih, Denny
Wahyuningsih, Mae Sri Hartati
peppermint oil - acute single bout of eccentric exercise - glucose transporter-4 - catalase - hydrogen peroxide
Peppermint oil is one of the essential oils with antioxidant activity that can reduce levels of reactive oxygen species (ROS). An acute single bout of eccentric exercise (ASBEE) is an acute exercise activity that can lead to increased ROS and cause skeletal muscle injury. This study aimed to assess the effect of peppermint oil in experimental animals induced with ASBEE with the purpose to measure catalase, hydrogen peroxide (H2O2) blood and glucose transporter-4 (GLUT-4) expression of skeletal muscle cells. A total of 30 Wistar rats (Rattus norvegicus) aged 20-24 weeks, weighing 160-350 g were divided into six groups i.e. T1 (n =5), T2 (n =4) and T3 (n =5) given peppermint oil orally at different dose of 0.25, 0.5 and 1.0 g/kg, respectively, one hour before inducing with ASBEE; C0 (n=5) not given peppermint oil and not induced with ASBEE; CA (n=5) not given peppermint oil and induced with ASBEE and CE (n=5) given vitamin E 400 mg/kg one h before induced with ASBEE. ASBEE induction was done by downhill running on a rat treadmill -50 with a load index of 70% VO2 max for 30 min. Twenty four h after induction of ASBEE, blood samples and muscle tissue were taken for examination of catalase, H2O2 and GLUT-4 expression. The results showed increased levels of blood catalase and decreased blood H2O2 levels in groups T1, T2, T3, and CE. The opposite occurred in the group CA. The GLUT-4 expression did not show any significant difference between groups. It was concluded that peppermint oil can improve the condition of oxidative stress caused by ASBEE.
Universitas Gadjah Mada
2018-10-05
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/25959
10.19106/JMedScie/005003201802
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 3 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 3 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/25959/pdf
Copyright (c) 2018 Dewi Aryanti, Denny Agustiningsih, Mae Sri Hartati Wahyuningsih
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/26048
2019-08-05T07:20:12Z
bik:ART
The SLCO1B1*15 haplotype associated with lower clinical outcome in Indonesian tuberculosis patients
Ang, Sunarto
Nugroho, Akhmad Kharis
Sadewa, Ahmad Hamim
Hakim, Lukman
Mustofa, .
SLCO1B1*15 haplotype; rifampin; tuberculosis; clinical outcome
Rifampin is one of first-line drugs for the treatment of tuberculosis. In Indonesia nearly alltuberculosis patients show lower rifampin plasma concentrations possibly due to genetics.Rifampin is a substrate of the organic anion-transporting polypeptide 1B1 (OATP 1B1)encoded by the solute carrier organic anion transporter family member 1B1 (SLCO1B1).This study aimed to identify haplotype polymorphisms of tuberculosis drug transporterswith an impact on clinical outcome in tuberculosis patients. Thirty-six patients from AbdulWahab Sjahranie General Hospital, Samarinda, East Kalimantan were involved in thestudy. Buffy coat from patient blood samples were tested for SLCO1B1 and SLCO1B3polymorphisms by RFLP and ARMS PCR, whereas the clinical outcome was examinedbased on the sputum conversion. The frequency of patients with SLCO1B1*15 haplotypewas 63.9%. The SLCO1B1*15 haplotype was associated with susceptibility to failureof clinical outcome (p=0.005; RR=4.52; 95% CI: 1.22-16.64). The OATP1B1*15haplotype revealed that the failure of clinical outcome was markedly increased comparedto the three other haplotypes. These results suggest that the SLCO1B1*15 haplotypeis an important predisposing factor for lower clinical outcome. Our data indicate thatindividualized treatment should be considered for Indonesian tuberculosis patients basedon genetics characteristics of patients.
Universitas Gadjah Mada
2018-03-26
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/26048
10.19106/JMedSci005001201806
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 1 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 1 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/26048/pdf
Copyright (c) 2018 Sunarto Ang, Akhmad Kharis Nugroho, Ahmad Hamim Sadewa, Lukman Hakim, . Mustofa
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/26241
2019-08-08T05:40:18Z
bik:ART
The role of Malassezia sp, sebum level and Trans Epidermal Water Loss (TEWL) toward the dandruff severity between hijab and non hijab wearing subjects
Etnawati, Kristiana
Siswati, Agnes Sri
Pudjiati, Satiti Retno
Susetiati, Devi Artami
Adiwinarni, Dwi Retno
Purbananto, Affendi
dandruff severity - Malassezia sp - sebum - TEWL - hijab
Dandruff is a common symptom in adolescence, which possibly due to increasing ofMalassezia sp. colonization, sebum level as well as skin hydration. Wearing hijab ispredicted to increase the humidity and sebum level lead to increase the number ofMalassezia sp. and dandruff severity. A case-control study was conducted on 19 femalewith dandruff who wear hijab and 19 female with dandruff who do not wear hijab, agebetween 18 and 27 years old. The dandruff severity was defined clinically using ascoring system (0-100), only subjects with minimum score of 28 will be included in thisstudy. The sebum and trans epidermal water loss (TEWL) levels were measured withSebumeter and Tewameter from Courage Khazaka. The Malassezia sp. was examinedusing microscopic examination of the squama and culture in the Saboraud medium.There were no significant differences found between hijab and non-hijab groups fordandruff severity. No difference found between two groups for sebum level, Malasseziasp. number microscopically, and Malassezia sp. colonization. Only TEWL level in hijabgroup that was found to be higher than non-hijab group. Wearing hijab does not increasethe dandruff severity, sebum level and colonization number of Malassezia sp. Wearinghijab is found to increase the TEWL level.
Universitas Gadjah Mada
2018-11-09
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/26241
10.19106/JMedScie/0050032018011
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 3 (2018)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 50, No 3 (2018)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/26241/pdf
Copyright (c) 2018 Kristiana Etnawati, Agnes Sri Siswati, Satiti Retno Pudjiati, Devi Artami Susetiati, Dwi Retno Adiwinarni, Affendi Purbananto
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/26422
2021-01-20T07:41:54Z
bik:ART
Incidental bladder wall alteration as a surgical complication risk factor in Indonesian benign prostate hyperplasia patient
Krisna, Daniel Mahendra
Maulana, Akhada
Kresnoadi, Erwin
benign prostate hyperplasia; bladder wall alteration; complication; comorbid; trans urethra resection of prostate;
Bladder obstruction due to benign prostatic hyperplasia (BPH) causes bladder walls alteration such as trabecular and diverticula. Although it is asymptomatic, however it affects the surgery complication. The aim of this study was to evaluate the correlation between bladder wall alteration and post-surgery complications in BPH patients. It was a descriptive observational study with cross-sectional design involving BPH patients who underwent histopathological examination and surgical intervention in the Bhayangkara Hospital Mataram from January 2010-December 2014. Data of the patients including age, place of birth, intraoperative finding, comorbid condition, and post-surgery complication were obtained from the medical record. The relationship between variables was analyzed using Pearson correlation test and linear regression test.A total 114 subjects were involved in this study with the most common age was in the range 60-69 years (43.0%). Seventy-fivesubjects (74.3%) had trans urethra resection of prostate (TURP). Trabecula (28.7%), diverticula (4.0%), and bladder stone (10.9%) were found when the surgical intervention performed. The most common post-surgical complication was chip or clot retention (56.4%). Significantly relationship betweenintraoperative finding with post-surgical complication was observed (r= 0.265; R2 = 0.07; p<0.05). In conclusion, there is correlation between bladder wall alteration and post-surgicalcomplication in BPH patients. Early bladder wall alteration screening is suggested in BPH patients before surgical intervention performed.
Universitas Gadjah Mada
2020-10-06
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/26422
10.19106/JMedSci005204202005
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 52, No 4 (2020)
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 52, No 4 (2020)
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/26422/pdf
Copyright (c) 2020 Daniel Mahendra Krisna, Akhada Maulana, Erwin Kresnoadi
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/26601
2019-11-14T07:31:53Z
bik:ART
Critical appraisal of neuropathic pain guidelines in Asia
Pinzon, Rizaldy Taslim
Sanyasi, Rosa De Lima Renita
systematic review, neuropathic pain, guidelines, Asia
Neuropathic pain (NP) is type of chronic pain that is common and often difficult to treat. Clinicians may be guided by a number of published guidelines and algorithms for the management of neuropathic pain. It is important for every clinician to know the quality of guidelines. The availability of current guideline in Asian countries is not well understood. Critical appraisal of NP guidelines in Asia has not performed, yet. The aim of this study was to appraise the quality of pharmacological treatment from neuropathic pain guidelines in Asia. Systematic searches were conducted by using combination of keywords i.e NP, Asia, and guideline. Guidelines evaluation was using appraisal of guidelines for research & evaluation II (AGREE II) instrument. The result interpreted as (i) a strongly recommended for use in practice if most domains scored above 50%; (ii) recommended for use with some modification if most domains scored between 30% to 50%; or (iii) not recommended for use in practice if most domains scored below 30%. After matched to inclusion and exclusion criteria there were 5 guidelines left: a guideline from Philippines, China, South Korea, Malaysia and Middle East. All of guidelines are recommended for use with some modification. The guidelines mention that first line treatment for NP are tricyclic antidepressants, selective norepinephrine reuptake inhibitor (SNRI) and alpha 2-delta ligand calcium channel blocker (CCB). Most of the evidences come from peripheral NP. There were limited evidences for the treatment of central neuropathic pain (central post stroke pain and pain after spinal cord injury). All the existing guideline mention, that the first line treatment for neuropathic are the tricyclic antidepressants, SNRI and alpha 2-delta ligand CCB.
Universitas Gadjah Mada
2019-04-11
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/26601
10.19106/JMedSci005101201909
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 51, No 1 (2019); 73-81
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 51, No 1 (2019); 73-81
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/26601/pdf
Copyright (c) 2019 Rizaldy Taslim Pinzon, Rosa De Lima Renita Sanyasi
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/27679
2019-11-27T00:49:27Z
bik:ART
The effect of L-arginine therapy on hepatocyte cells damage in mice (Mus musculus) model of preeclampsia
Soetrisno, S
Arinekso, Bambang
Sulistyowati, Sri
L-arginine; hepatocytes cell; preeclampsia; mouse model; animal model
Preeclampsia is one of the main contributors on morbidity and mortality of mother and fetus. Imbalance of body antioxidants and oxidants has a role on endothelial destruction causing endothelial dysfunction which leads to preeclampsia. L-arginine administration is indicated to be able to fix the remodelling process of hepatocytes which was damaged on preeclampsia. The aim of this study was to investigate the effect of L-arginine on hepatocyte cells damage on mouse (Mus musculus) model of preeclampsia. Experimental study was conducted in the Experimental Animal Warehouse and Biomedical Laboratory, Faculty of Veterinary Medicine, Airlangga University, Surabaya. A total of 30 pregnant mice were divided into three groups with 10 mice in each group i.e. normal, preeclampsia model and preeclampsia model with L-arginine therapy pregnant mice. Preparates were taken from dissected mice liver, with prior paraffin block and were then stained with hematoxylin-eosin. Results were analyzed semi quantitatively with Manja Roenigk hepar histology scoring method. Data were analyzed using Kruskal-Wallis and Man-Whitney test. Mean of hepar histopathology score of preeclampsia model with L-arginine therapy (40.80±1.17/µm2) was significantly lower compared with preeclampsia model pregnant mice (58.80±4.23/µm2) (p<0.05), however it was significantly higher compared with normal pregnant mice (18.40±2.41/µm2) (p<0.05). It was indicated that the L-arginine therapy on preeclampsia mouse model can reduce the score of hepar histopathology. In conclusion, L-arginine administration can reduce the hepatocyte cells damage of mouse due to preeclampsia.
Universitas Gadjah Mada
2019-09-24
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/27679
10.19106/JMedSci005104201905
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 51, No 4 (2019); 316-324
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 51, No 4 (2019); 316-324
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/27679/pdf
Copyright (c) 2019 S Soetrisno, Bambang Arinekso, Sri Sulistyowati
http://creativecommons.org/licenses/by-nc/4.0
oai:jurnal.ugm.ac.id:article/27787
2019-11-26T06:35:56Z
bik:ART
Effect of tagitinin C isolated from Tithonia diversifoli (Hemsley) A Gray on migration activity and TGF-β1 levels on keloid fibroblast
Santi, Elvira
Wahyuningsih, Mae Sri Hartati
Budiyanto, Arief
Keloid fibroblast; Proliferation; Tagitinin C; TGF-β1 Level
Keloid is the formation of excessive scar tissue characterized by fibroblast hiperproliferations and collagen deposits that are similar with cancer cells. Tagitinin C is proven can inhibit proliferation and deposition of keloids collagen fibroblast. However, the mechanism of action of tagitinin C in migration activities and TGF-β1 levels of keloid fibroblasts has not been proved, yet. This study aimed to investigate the effects of tagitinin C isolated from Tithonia diversifoli (Hemsley) on migration activity and TGF-β1 expression of keloid fibroblast. This was quasi experimental study with post test only controlled group design using keloid fibroblasts isolated from keloid patients. The migration activity were performed by scratch assay and TGF-β1 levels were measured using an ELISA kits. Isolate tagitinin C was more active inhibit fibroblast keloid migration compare to the control groups (p<0.05) after 48 h incubation. TGF-β1 levels after incubation with isolate tagitinin C was lower then control group (p<0.05). In conclusion, isolate tagitinin C can inhibit migration and reduce TGF-β1 levels on keloid fibroblast
Universitas Gadjah Mada
2019-08-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://jurnal.ugm.ac.id/bik/article/view/27787
10.19106/JMedSci005103201901
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 51, No 3 (2019); 188-194
Journal of the Medical Sciences (Berkala Ilmu Kedokteran); Vol 51, No 3 (2019); 188-194
2356-3931
eng
https://jurnal.ugm.ac.id/bik/article/view/27787/pdf
Copyright (c) 2019 Elvira Santi, Mae Sri Hartati Wahyuningsih, Arief Budiyanto
http://creativecommons.org/licenses/by-nc/4.0
4a52ffdaf5caaa5951d5b2b86cf57839